Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses
Title : Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses Corporate Authors(s) : ... Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses. ... Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses ... "Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses" vol. 21, no. 2, 2008. Export RIS ...
Interim Guidance for Interpretation of Zika Virus Antibody Test Results | MMWR
CDC provides recommendations for interpreting Zika test results. ... CDC provides recommendations for interpreting Zika test results ... Negative for Zika virus AND negative for dengue virus. Not indicated. No evidence of recent Zika virus or dengue virus ... plaque reduction neutralization test.. *For persons with suspected Zika virus disease, Zika virus real-time reverse ... No evidence of Zika virus or dengue virus infection. Positive for Zika virus AND negative for dengue virus. Not yet performed. ...
Pleurodynia: Background, Pathophysiology, Etiology
Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses. Viral Immunol. 2008 Jun. 21(2): ... An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am J Epidemiol. 1966 ... Coxsackie B virus infections in New Zealand patients with cardiac and non-cardiac diseases. J Med Virol. 1983. 11(2):131-7. [ ... First report of a Chinese strain of coxsackie B3 virus infection in a newborn in Germany in 2011: a case report. J Med Case Rep ...
2015-16 Zika virus epidemic - Wikipedia
Plaque-reduction neutralization tests (PRINT) can be performed and may be specific. The Zika virus can be identified by RT-PCR ... "Revised diagnostic testing for Zika, chikungunya, and dengue viruses in US Public Health Laboratories" (PDF). Division of ... RT-PCR testing of serum and tissue samples can be used to detect the presence of the Zika virus. However, the RT-PCR test with ... The CDC authorized emergency use of a Zika Virus RNA Qualitative test on April 28 to detect Zika virus in the blood of patients ...
Evidence of Locally Acquired Dengue Virus Infection, Arizona
This report details the identification of the first locally acquired human dengue virus infection in Maricopa County, Arizona. ... CDC Arboviral Diseases Branch confirmed DENV-3 by plaque reduction neutralization testing. The person with the positive test ... On November 7, 2022, dengue virus (DENV), which is not endemic in the continental United States,[1] was identified in a ... First Pill for Dengue Shows Promise in Human Challenge Trial * Dengue Will Take Off in Southern Europe, US, Africa This ...
ArboCat Virus: Trubanaman (TRUV)
... dengue virus, types 1-4 and Bebaru. Neutralization tests showed no relation to any of the viruses previously isolated in ... Virus Sections. Virus Name/Prototype. Original Source. Method of Isolation. Virus Properties. Antigenic Relationship. Biologic ... Section V - Antigenic Relationship and Lack of Relationship to Other Viruses. No hemagglutinin has been prepared from MRM3630, ... showed a relationship to Mapputta virus (MRM186) but not to the following viruses isolated in Australia - Sindbis, Getah, Ross ...
Dengue and Dengue Hemorrhagic Fever
Louis encephalitis viruses.(35) Complement fixation and neutralization antibody tests are more specific than hemagglutination ... Dengue and dengue hemorrhagic fever (DHF) result from infection by any of four serotypes of dengue viruses. Transmission occurs ... In a review of 131 patients from whom dengue virus was isolated at the Centers for Disease Control, Dengue Branch, 96% of the ... Why dengue hemorrhagic fever in Cuba? * Individual risk factors for dengue hemorrhagic fever/dengue shock syndrome. Trans R Soc ...
Epidemiology of Dengue, Chikungunya, and Zika Virus Disease in U.S. States and Territories, 2017 in: The American Journal of...
We describe the epidemiology of dengue, chikungunya, and noncongenital Zika virus disease cases reported from U.S. states and ... and Zika virus disease (n = 620, 93%) cases were reported from Puerto Rico. Temporally, the highest number of Zika virus ... Dengue and chikungunya cases followed a more seasonal pattern, with higher case numbers from July through September. Travelers ... the United States and residents of areas with active virus transmission should be informed of both the ongoing risk from dengue ...
Call Transcript: February 25, 2016|Clinicians Outreach and Outreach Communication (COCA)
... testing of growth and placental tissue and cord tissue for Zika virus RNA; and testing of cord serum for Zika and dengue virus ... Plaque Reduction Neutralization Test or PRNT can be performed to measure virus specific neutralizing antibodies to Zika virus ... A positive Zika virus test is defined as either Zika virus RNA detected on RT-PCR or Zika virus IgM positive with confirmatory ... If Zika virus RNA is not detected and symptoms have been present for four or more days, serum may be tested for Zika and dengue ...
WHO EMRO | Zika virus: no cases in the Eastern Mediterranean Region but concerns remain | Volume 22, issue 5 | EMHJ volume 22,...
... dengue, yellow fever, West Nile viruses). The plaque-reduction neutralization test (PRNT) for virus-specific neutralizing ... Zika virus: following the path of dengue and chikungunya? Lancet. 2015 Jul 18;386(9990):243-4. PMID:26194519 ... Preventing or limiting the transmission of dengue, chikungunya and Zika viruses is completely dependent on the control of ... Maladie à virus Zika : malgré labsence de cas dans la Région de Méditerranée orientale les craintes persistent. RÉSUMÉ À la ...
Vital Signs: Update on Zika Virus-Associated Birth Defects and Evaluation of All U.S. Infants with Congenital Zika Virus...
CDC report highlights birth defect risks of Zika virus during pregnancy. ... CDC report highlights birth defect risks of Zika virus during pregnancy. ... AND Zika virus plaque reduction neutralization test [PRNT] titer ≥10, regardless of dengue virus PRNT value; or negative Zika ... virus IgM, AND positive or equivocal dengue virus IgM, AND Zika virus PRNT titer ≥10, regardless of dengue virus PRNT titer). ...
Call Transcript: February 25, 2016|Clinicians Outreach and Outreach Communication (COCA)
... testing of growth and placental tissue and cord tissue for Zika virus RNA; and testing of cord serum for Zika and dengue virus ... Plaque Reduction Neutralization Test or PRNT can be performed to measure virus specific neutralizing antibodies to Zika virus ... A positive Zika virus test is defined as either Zika virus RNA detected on RT-PCR or Zika virus IgM positive with confirmatory ... If Zika virus RNA is not detected and symptoms have been present for four or more days, serum may be tested for Zika and dengue ...
Zika Virus Spreads to New Areas - Region of the Americas, May 2015-January 2016 | MMWR
Transmission of Zika virus has been reported in 20 countries in the Americas. ... Transmission of Zika virus has been reported in 20 countries in the Americas. ... dengue and yellow fever viruses). Virus-specific cross-neutralization testing can be used to discriminate between cross- ... There is no commercially available test for Zika virus. Zika virus testing is performed in the United States at CDC and four ...
IJERPH | Free Full-Text | A Review of Vaccine Approaches for West Nile Virus
... according to the West Nile virus disease cases and deaths reported to the CDC by year and clinical presentation, 1999-2012, by ... the establishment and recent spread of lineage II WNV virus strains into Western Europe and the presence of neurovirulent and ... The West Nile virus (WNC) first appeared in North America in 1999. The North American lineages of WNV were characterized by the ... Similar vaccines have also been generated and tested for all four Dengue virus serotypes [168,169,170,171,172,173]. The ...
Update: Interim Guidelines for Health Care Providers Caring for Pregnant Women and Women of Reproductive Age with Possible Zika...
CDC provides recommendations for preventing transmission of Zika virus among health care workers treating pregnant women. ... CDC provides recommendations for preventing transmission of Zika virus among health care workers treating pregnant women. ... Arboviral Diseases and Dengue Branches. Updated diagnostic testing for Zika, chikungunya, and dengue viruses in US Public ... Plaque-reduction neutralization testing (PRNT) can be performed to measure virus-specific neutralizing antibodies to Zika virus ...
Zika - PDPH Health Information Portal
Plaque-reduction neutralization tests (PRNT) that help differentiate flavivirus infections (e.g., Zika, dengue, West Nile, ... Zika Testing Update Advisory Oct 22, 2018. Zika Virus Update: Epidemiology, Laboratory Testing, and Reporting Advisory Aug 11, ... dengue and chikungunya). If IgM testing for dengue and chikungunya is indicated, commercial laboratory testing should be ... While the PADOH laboratory can conduct PCR testing for dengue and chikungunya, they do not offer dengue and chikungunya IgM ...
Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes |...
... with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). This was ... Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (m.o.i. of 2) had dengue-3 ... In cultures simultaneously infected with two dengue virus types, one virus type was excluded from replication in most cells. A ... Control cultures infected with dengue-3 at the same multiplicity contained dengue-3 antigen in greater than 90% of the cells. ...
Low seroprevalence rates of Zika virus in Kuala Lumpur, Malaysia - UM Research Repository
dengue virus; Malaysia; neutralization test; NS1 blockade-of-binding ELISA; seroprevalence; Zika virus. ... Reactive samples were confirmed using neutralization assays against ZIKV and the four dengue virus (DENV) serotypes. A sample ... Background: Zika virus (ZIKV) is believed to be endemic in Southeast Asia. However, there have been few Zika cases reported to ... Methods: To determine Zika virus (ZIKV) seroprevalence in Kuala Lumpur, Malaysia, 1085 serum samples from 2012, 2014-2015 and ...
Email Updates: November 21 2016|Clinicians Outreach and Communication Activity (COCA)
Clinical Evaluation and Testing. UPDATED: Guidance for U.S. Laboratories Testing for Zika Virus Infection The guidance was ... Situational Update for Clinicians about Severe Monkeypox Virus Infections *What Clinicians Need to Know about Dengue in the ... The updated guidance also specifies that plaque reduction neutralization testing (PRNT) confirmation is currently not routinely ... Testing for Zika Virus. Contact your state or local health department to facilitate testing. ...
The Immunogenicity and Safety of CYD-Tetravalent Dengue Vaccine (CYD-TDV) in Children and Adolescents: A Systematic Review -...
Neutralization Tests 21% * Dengue Virus 19% * Indonesia 19% * Dengue 18% * Randomized Controlled Trials 10% ... Dive into the research topics of The Immunogenicity and Safety of CYD-Tetravalent Dengue Vaccine (CYD-TDV) in Children and ... The Immunogenicity and Safety of CYD-Tetravalent Dengue Vaccine (CYD-TDV) in Children and Adolescents: A Systematic Review. ...
Value Set Concept Details
Epidemiological and ecological characteristics of past dengue virus infection in Santa Clara, Peru<...
... for DEN virus IgG antibody. Antibody identity was verified as DEN by plaque reduction neutralization test. Data on individuals ... for DEN virus IgG antibody. Antibody identity was verified as DEN by plaque reduction neutralization test. Data on individuals ... for DEN virus IgG antibody. Antibody identity was verified as DEN by plaque reduction neutralization test. Data on individuals ... for DEN virus IgG antibody. Antibody identity was verified as DEN by plaque reduction neutralization test. Data on individuals ...
Pleurodynia Differential Diagnoses
Guidelines for Plaque-Reduction Neutralization Testing of Human Antibodies to Dengue Viruses. Viral Immunol. 2008 Jun. 21 (2): ... An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am J Epidemiol. 1966 ... Coxsackie B virus infections in New Zealand patients with cardiac and non-cardiac diseases. J Med Virol. 1983. 11(2):131-7. [ ... First report of a Chinese strain of coxsackie B3 virus infection in a newborn in Germany in 2011: a case report. J Med Case Rep ...
GtR
Refinement of plaque reduction neutralisation test (PRNT) for Zika virus Refined methodology to speed up read-out from PRNT ... The study demonstrated cross-reactivity in ELISA testing between Zika and Dengue virus. The study and subsequent publications ... We aim to refine and validate more specific serological tests (Plaque Reduction Neutralisation. Test [PRNT] and Non-structural ... Tested, improved and optimize a new assay using a portable PCR machine for Zika virus Diagnosis using body fluid samples. ...
Viruses | Free Full-Text | Development, Characterization, and Application of Two Reporter-Expressing Recombinant Zika Viruses
As compared to the parental virus, the two reporter-expressing ZIKVs grew to lower titers with slower growth kinetics and ... is an enveloped virus with an ~10.8 kb plus-strand RNA genome that can cause neurological disease. To facilitate the ... they displayed a genome-wide viral protein expression profile identical to that of the parental virus, except for two ... Zika virus (ZIKV), a mosquito-borne transplacentally transmissible flavivirus, ...
CDC Science Clips
... namely dengue, Zika and West Nile viruses. RESULT: Of the total samples tested, 10 (0.61%) were confirmed to be IgG positive ... Any serum sample testing positive by ELISA was confirmed by plaque reduction neutralization test (PRNT). In addition, ... Nine (0.5%) samples were antibody positive for dengue virus, 15(0.9%) forWest Nile virus and 7 (0.4%) for Zika virus by PRNT. ... Viral load coverage (testing of eligible PLHIV) and viral suppression were calculated at select time points. Qualitative data ...
RJPPD - Zika Virus: A Comprehensive Review
... is an arthropod-borne virus (arbovirus) belonging to the Flavivirus genus and the Flaviviridae family. Aedes mosquito species ... Guidelines for plaque-reduction neutralization testing of human antibodies to dengue viruses. Viral Immunol 2008; 123-132. 25. ... Texas lifestyle limits transmission of dengue virus. Emerg Infect Dis 2003; 9:86-89. 34. Heintze C, Velasco Garrido M, Kroeger ... Virus and antibody dynamics in acute West Nile virus infection. J Infect Dis 2008; 984-99 24. Roehrig JT, Hombach J, Barrett AD ...
Pesquisa | Portal Regional da BVS
Reporter virus particle neutralization assays after depletion of anti-DENV-2 nAbs demonstrated that the nAb response to DENV-1 ... Vacinas contra Dengue , Vírus da Dengue , Dengue , Adolescente , Adulto , Anticorpos Neutralizantes , Anticorpos Antivirais , ... RESULTS: Microneutralization tests using postvaccination serum showed comparable neutralization against diverse DENV-1-4 ... Vacinas contra Dengue , Vírus da Dengue , Dengue , Humanos , Anticorpos Neutralizantes , Vacinas Combinadas , Anticorpos ...