Biodegradable dendritic positron-emitting nanoprobes for the noninvasive imaging of angiogenesis | PNAS
Cell-based assays of the 125I-labeled dendritic nanoprobes using αvβ3-positive cells showed a 6-fold increase in αvβ3 receptor- ... Cell uptake studies. (A) Percentage total cell-associated fraction, cell-internalized fraction, and surface-bound fraction for ... β3-negative cells showed no enhancement of cell uptake over time. In vivo biodistribution studies of 76Br-labeled dendritic ... excess of unlabeled targeted dendritic nanoprobe) and in αvβ3-negative M21L cells, consistent with receptor-mediated cell ...
sphingosine 1-phosphate | PNAS
S1PR1-mediated IFNAR1 degradation modulates plasmacytoid dendritic cell interferon-α autoamplification John R. Teijaro, Sean ...
The V2 loop of HIV gp120 delivers costimulatory signals to CD4+ T cells through Integrin α4β7 and promotes cellular activation...
Specialization of mucosal follicular dendritic cells revealed by mucosal addressin-cell adhesion molecule-1 display. J. Immunol ... Integrin α4β7, is a cell-surface receptor that facilitates CD4+ T cell homing to PPs, MLNs, and LP (7⇓-9). Memory CD4+ T cells ... Human Blood Samples and Primary Cell Preparation.. CD4+ T cells were obtained by negative selection (,95% purity) (Stem Cell ... It is also expressed on follicular dendritic cells (FDCs) in MLNs (18). MAdCAM binding to α4β7 on CD4+ T cells delivers a ...
A functional mammalian display screen identifies rare antibodies that stimulate NK cell-mediated cytotoxicity | PNAS
Cd137 stimulation enhances cetuximab-induced natural killer: Dendritic cell priming of antitumor T-cell immunity in patients ... Like T cells, natural killer (NK) cells are cytotoxic effector cells that play a key role in the antitumor response. To advance ... NK cells have the innate ability to identify and kill target cells. Antibodies that bind to NK cell surface proteins are ... antibodies to redirect NK cell-mediated cytotoxicity toward CD20+ B cell lymphoma cells and HER2+ breast cancer cells. While ...
Edaravone alleviates Alzheimer's disease-type pathologies and cognitive deficits | PNAS
In human neuroblastoma SH-SY5Y cells, Edaravone dose-dependently protected neurons from cell death (Fig. S2 A and B) and ... 5H) groups, and the number of dendritic spines detected by Golgi staining in the hippocampus was also significantly increased ... S2 D and E), cell death (Fig. S2 F and G), and ROS production (Fig. S2H) triggered by Aβ. These results suggest that Edaravone ... I-K) Western blots and quantitative analysis for sAPPα (I), sAPPβ (I), CTFβ (J), CTFα (J), and BACE1 (K) in SH-SY5Y-APP695 cell ...