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*  Patente US5538871 - In situ polymerase chain reaction - Google Patentes
... a process of in vitro enzymatic amplification of specific nucleic acid sequences within the cells where they originate, can be ... The cells may be in aqueous suspension or may be part of a histochemical section or cytochemical smear. Preferably the cells ... Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US4965188 *. 17 Jun 1987. 23 Oct 1990. Cetus ... Cells of the stable human cervical cancer cell line, SiHa (ATCC HTB 35), containing one integrated copy of human papilloma ...
http://www.google.es/patents/US5538871
*  A BAC transgenic analysis of the Mrf4/Myf5 locus reveals interdigitated elements that control activation and maintenance of...
Spotlight on Parkinson's and stem cells. Malin Parmar shines the spotlight on the current status and future prosepcts of stem ... We show that a clone containing Myf5 and 140 kb of upstream sequences is sufficient to recapitulate the known expression ... REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity ... ImaEdge - a platform for quantitative analysis of the spatiotemporal dynamics of cortical proteins during cell polarization. ...
http://dev.biologists.org/content/128/10/1857
*  Patent US5200313 - Nucleic acid hybridization assay employing detectable anti-hybrid antibodies - Google Patents
Cell. 33:865]. The probe encompasses about two-thirds of the 23s RNA sequence from the 3'-hydroxyl and is cloned into an M13 ... A DNA probe is cloned into the vector which is then propagated in a bacterial host. Multiple RNA copies of the cloned DNA probe ... Mice are immunized with DNA.RNA hybrid as described in Example 1 and spleen cells are fused with SP 2/0-Ag14 myeloma cells ( ... to aid in the diagnosis of genetic disorders such as sickle cell anemia and thalassemia, and to detect cancerous cells. A ...
http://www.google.ca/patents/US5200313
*  Bacterial artificial chromosome - Wikipedia
These constructs are referred to as "infectious clones", as transfection of the BAC construct into host cells is sufficient to ... for antibiotic resistance; some BACs also have lacZ at the cloning site for blue/white selection.. T7 & Sp6. phage promoters ... The bacterial artificial chromosome's usual insert size is 150-350 kbp.[4] A similar cloning vector called a PAC has also been ... for partitioning F plasmid DNA to daughter cells during division and ensures stable maintenance of the BAC.. A selectable ...
https://en.wikipedia.org/wiki/Bacterial_artificial_chromosome
*  Frontiers | Screening of an E. coli O157:H7 Bacterial Artificial Chromosome Library by Comparative Genomic Hybridization to...
... one involved three rounds of adherence assays to bovine primary rectal epithelial cells while the other competed the clones ... The library contains 1152 clones averaging 150 kbp. To verify the library, clones containing a complete locus of enterocyte ... one involved three rounds of adherence assays to bovine primary rectal epithelial cells while the other competed the clones ... However, conjugation of one of the BAC clones into a strain containing a partial LEE deletion restored type III secretion. 384 ...
https://www.frontiersin.org/articles/10.3389/fmicb.2011.00168/full
*  DNA restriction enzymes facts, information, pictures | Encyclopedia.com articles about DNA restriction enzymes
Molecular Cell Biology, 4th ed. New York: W. H. Freeman, 2000.. Old, R. W., and S. B. Primrose. Principles of Gene Manipulation ... Thus, the fifth restriction enzyme from E. coli is called EcoRV (pronounced e, ko, r five). Besides cloning , restriction ... The Cell: A Molecular Approach. Washington, DC: ASM Press, 1997.. Kreuzer, Helen, and Adrianne Massey. Recombinant DNA and ... They are used to digest the DNA from the experimental organism, in order to prepare the DNA for cloning. Then a bacterial ...
https://www.encyclopedia.com/science-and-technology/biology-and-genetics/genetics-and-genetic-engineering/dna-restriction
*  Microbiology Society Journals | Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces...
... clone of HVT was constructed. DNA from two independent BAC clones, upon transfection into chicken embryo fibroblasts, produced ... Viruses derived from the BAC clones were stable during in vitro passage, but showed differences in in vitro growth kinetics ... In spite of the difference in in vitro growth, viruses from both clones induced 100 % protection against infection by the ... Furthermore, the availability of the BAC clones enables use of HVT as a vector for expressing foreign genes. ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81498-0
*  'clone cells' Protocols and Video...
... clone cells' include 'Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human ... Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes ... Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & ... Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus. Xuelian Wang1 ...
https://www.jove.com/keyword/clone+cells
*  Clone cell phone number phone Jobs, Employment | Freelancer
Search for jobs related to Clone cell phone number phone or hire on the world's largest freelancing marketplace with 15m+ jobs ... Other jobs related to clone cell phone number phone. clone phone number , tell network cell phone number , cell phone number ... cell phone number grabber , cell phone number verifacation , cell phone number verification , clone cell phone number , phone ... view indian cell phone number , upload via cell phone number , cell phone number reverse lookup ukraine , blackberry app clone ...
https://www.freelancer.com/job-search/clone-cell-phone-number-phone/490/
*  jira] [Created] (HBASE-15721) Optimization in cloning cells into MSLAB
... cell.getValueLength()); pos = appendKeyTo(cell, output, pos); pos = CellUtil.copyValueTo(cell, output, pos); if ((cell. ... Before cells added to memstore CSLM, there is a clone of cell after copying it to MSLAB chunk area. This is done not in an ... final Cell cell, final byte[] output, final int offset) { int pos = offset; pos = Bytes.putInt(output, pos, keyLength(cell)); ... Anoop Sam John created HBASE-15721: -------------------------------------- Summary: Optimization in cloning cells into MSLAB ...
http://mail-archives.apache.org/mod_mbox/hbase-dev/201604.mbox/%3CJIRA.12962920.1461744353000.44204.1461744372893@Atlassian.JIRA%3E
*  Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586 | Cancer Research
Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586. Toby G. Rossman, Lawrence Prutkin and Victor R. Wheatley ... The cells are grown in a modified CMRL-1415 medium supplemented with 10% fetal calf serum. Clones tend to fall into two classes ... Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used ... Preliminary studies on the control of differentiation in Class 1 clones suggest that a minimum cell density is required before ...
http://cancerres.aacrjournals.org/content/37/2/557
*  Human embryo stem cells cloning breakthrough - NHS
Human embryonic stem cells created from adult tissue for first time,' The Guardian reports, while the Daily Mail's front page ... leads with the somewhat fanciful warning that new research raises the 'spectre of cloned babies'… ... When these stem cells were tested, researchers found that the cells were able to develop into other types of cells in a manner ... The embryonic stem cells were able to develop into several different types of cells, including heart cells. They were also ...
https://www.nhs.uk/news/genetics-and-stem-cells/human-embryo-stem-cells-cloning-breakthrough/
*  Melanoma Patient Tumor Free in T-Cell Clone Study
Cloned tumor-fighting immune cells cured one of 11 patients with metastatic melanoma in a dramatic demonstration of the power ... Melanoma Patient Tumor Free in T-Cell Clone Study. Immune Therapy for Late-Stage Melanoma: No More Cancer in 1 of 11 Patients ... Successful treatment depended on isolating and cloning just the right kind of cancer-fighting immune cell. This happened in ... late-stage melanoma is cancer free 3.5 years after experimental treatment with clones of his own immune cells. ...
https://www.webmd.com/melanoma-skin-cancer/news/20120305/melanoma-patient-tumor-free-in-t-cell-clone-study
*  Wanted - T cell clones, rheumatoid arthritis
... Mr. D.N.C. Hunter dhunter at hgmp.mrc.ac.uk Tue Sep 9 09:24:57 EST 1997 *Previous ... I don't have much experience in T cell cloning and I am led to believe that it is very time consuming and not always successful ... Ideally I would like to use human T cell clones derived from subjects with rheumatoid arthritis. ... I am presently looking at cytokine mediated differential gene expression in T cells. ...
http://www.bio.net/bionet/mm/immuno/1997-September/010957.html
*  Human embryo stem cells cloning breakthrough | NHS Choices | Nursing Times
When these stem cells were tested, researchers found that the cells were able to develop into other types of cells in a manner ... The embryonic stem cells were able to develop into several different types of cells, including heart cells. They were also ... These are then fused with human cells - in this case skin cells - and the fused cell begins behaving in a similar way to an ... This was a laboratory study that aimed to produce embryonic stem cells from adult skin cells. Embryonic stem cells are unique ...
https://www.nursingtimes.net/home/behind-the-headlines/human-embryo-stem-cells-cloning-breakthrough/5058821.article
*  Developmental fate of a distinct class of chick myoblasts after transplantation of cloned cells into quail embryos | Development
After four days of growth, cells of the transplant-containing quail legs were grown as clones. Chick clones were located by ... Developmental fate of a distinct class of chick myoblasts after transplantation of cloned cells into quail embryos ... Developmental fate of a distinct class of chick myoblasts after transplantation of cloned cells into quail embryos ... Developmental fate of a distinct class of chick myoblasts after transplantation of cloned cells into quail embryos ...
http://dev.biologists.org/content/58/1/119
*  CiNii 論文 - Role of Pseudomonas aeruginosa Culture Filtrates in the Association, Invasion, and...
... and Cytotoxicity Against Cloned Cells from Murine Corneal Epithelium and KB Cells * * ISHINO Gou ... The effects of short-term contact lens wear on adherence of Pseudomonas aeruginosa to human corneal cells HARDING AS ... Relationship between cytotoxicity and corneal epithelial cell invasion by clinical isolates of Pseudomonas aeruginosa FLEISZIG ... Transformation of Mouse Peritoneal Macrophages and Bone Marrow Cells by Simian Virus 40 Takayama Hisao ...
https://ci.nii.ac.jp/naid/10013263688
*  First Embryonic Stem Cells Cloned From A Man's Skin | WUNC
... scientists in Scotland took the nuclear DNA from the cell of an adult sheep and put it into another sheep's egg cell that had ... And with a bit of coaxing, these cells could be turned into nerve cells, pancreas cells, liver cells, heart cells, just about ... But he says this does mean we could be getting closer to being able to go beyond cloned cell lines to cloning an entire human ... HARRIS: And used the DNA from those cells in this cloning process. They injected it into 77 human egg cells and managed to ...
http://wunc.org/post/first-embryonic-stem-cells-cloned-mans-skin
*  Pancreatic islet-specific T-cell clones from nonobese diabetic mice | PNAS
Comparison of a T Cell Clone and of T Cells from a TCR Transgenic Mouse: TCR Transgenic T Cells Specific for Self-Antigen Are ... We have produced a panel of islet-specific T-cell clones from nonobese diabetic (NOD) mice. These clones proliferate and make ... Pancreatic islet-specific T-cell clones from nonobese diabetic mice. K Haskins, M Portas, B Bergman, K Lafferty, and B Bradley ... Most of the clones respond to islet-cell antigen from different mouse strains but only in the presence of antigen-presenting ...
https://www.pnas.org/content/86/20/8000?ijkey=793d9d204ef1c2384b2d2d3436756f057092c9cf&keytype2=tf_ipsecsha
*  Stem Cells Cloned from Patient with Diabetes - MPR
In this cloning process, known as nuclear transfer, a donated ovum with all DNA removed had the woman's adult cells inserted ... Patients with diabetes may one day benefit from stem cell therapy, as research published in the journal Nature describes the ... This could lead to diabetic patients being able to produce their own cells that make insulin to replace their non-functioning ... "clone" cells .... READ FULL ARTICLE From Time ... That's a question that stem cell researchers - and policy ...
http://www.empr.com/medical-news/stem-cells-cloned-from-patient-with-diabetes/article/344475/
*  Enzyme accelerates malignant stem cell cloning in chronic myeloid leukemia
... has identified a key enzyme in the reprogramming process that promotes malignant stem cell cloning and the growth of chronic ... Enzyme accelerates malignant stem cell cloning in chronic myeloid leukemia. Wednesday, January 2, 2013. This shows chronic ... Genetic switch makes fat cells burn energy rather than store it. We now know how to turn fat cells into ones that burn calories ... has identified a key enzyme in the reprogramming process that promotes malignant stem cell cloning and the growth of chronic ...
http://www.labspaces.net/126086/Enzyme_accelerates_malignant_stem_cell_cloning_in_chronic_myeloid_leukemia
*  Clone (cell biology) - Wikipedia
Clone (B-cell biology) Cloning List of animals that have been cloned Polyclonal antibodies Polyclonal response "Clone ... so they are technically a single clone of cells. However, during course of cell division, one of the cells can get mutated ... bone marrow cells. Most other cells cannot divide indefinitely as after a few cycles of cell division the cells stop expressing ... A clone is a group of identical cells that share a common ancestry, meaning they are derived from the same cell. Clonality ...
https://en.wikipedia.org/wiki/Clone_(cell_biology)
*  Stem cells & cloning - Andrew Bartlett
Cloning Bill passes Senate - just. 7 November, 2006. / 81 Comments Despite a general expectation that the stem cell legislation ... Category: Stem cells & cloning. Pell and Parliamentary Privilege. 9 June, 2007. / 16 Comments ... Stem Cell fest gathers pace. 9 October, 2006. / 68 Comments This fortnight is the final sitting period before the stem cell ... Stem Cell Inquiry reports. 30 October, 2006. / 35 Comments The report of the Senate Committee examining the stem cell ...
http://andrewbartlett.com/category/the-senate/stem-cells/
*  Unexpectedly uneven: posttransplant skeletal distribution of hematopoietic stem cell clones | JEM
Unexpectedly uneven: posttransplant skeletal distribution of hematopoietic stem cell clones. Elliott J. Hagedorn, Leonard I. ... Unexpectedly uneven: posttransplant skeletal distribution of hematopoietic stem cell clones. Elliott J. Hagedorn, Leonard I. ... Other clones were more indiscriminant.. Using viral barcoding to monitor transplanted mouse HSC clones, Verovskaya et al. ... and colleagues suggests that the skeletal distribution of transplanted hematopoietic stem cell (HSC) clones is surprisingly ...
http://jem.rupress.org/content/211/3/384.2
*  Recent Articles | Cloning, Cell & Molecular Biology And Developmental Biology | The Scientist Magazine®
tags: cloning x cell & molecular biology x developmental biology x immunology x ... Single-cell genome analyses reveal the amount of mutations a human brain cell will collect from its fetal beginnings until ... Immune-like cells in the central nervous system are now recognized as key participants in the creation and maintenance of ... The discovery reveals the role of a growth factor and endothelial cells in thymus repair, and could have implications for ...
https://www.the-scientist.com/?articles.list/categoryNo/2625/category/The-Scientist/tagNo/435,4,5,12/tags/cloning,cell--amp--molecular-biology,developmental-biology,immunology/