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*  Localized hepatic lobular regeneration by central-vein-associated lineage-restricted progenitors | PNAS
Clones ranged from single cells to 408 (max). (C) Representative image across three channels (GFP, RFP, and CFP) showing large ... C) Approximate total clone number, measured by the clone density (number of clones per square millimeter) multiplied by total ... D) e15, P0, and adult mice differ in the number of clones associated with vasculature. (E) Large clones in both regenerating ... Representative image of RFP and CFP tetrachimeric clones from the regenerating area is shown. (B) Clone sizes of embryonic day ...
http://www.pnas.org/content/early/2017/03/21/1621361114/tab-figures-data
*  Stachel-independent modulation of GPR56/ADGRG1 signaling by synthetic ligands directed to its extracellular region | PNAS
Cloning and Purification of GPR56 Extracellular Domains from Insect Cells.. The following constructs were prepared and cloned ... Briefly, HEK293T cells were seeded in 96-well plates (10,000 cells in 0.1 mL DMEM + 10% FBS per well). After 12-18 h, cells ... A) Whole-cell lysate of HEK293T cells transfected with indicated mouse GPR56 constructs. (B) Cultured HEK293T cells previously ... Cells were lysed in the same total volume as the media in which they were cultured. Media and whole-cell lysate were subject to ...
http://www.pnas.org/content/114/38/10095
*  TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis | PNAS
Nine of the transgenic cell colonies were used as donor cells to produce cloned transgenic cattle. A total of 1,580 ... Although we detected an off-target mutation in one of 19 F-A locus gene-targeted cell clones (SI Appendix, Fig. S6 and Tables ... 2009) A targeted neuroglial reporter line generated by homologous recombination in human embryonic stem cells. Stem Cells 27(8 ... We created 13 transgenic cattle using M-S locus-targeted heterozygous colonies as donor cells. Heterozygous colonies of cells ...
https://www.pnas.org/content/112/13/E1530
*  Artificial cloning of domestic animals | PNAS
2003) Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle. Theriogenology ... 2013) Effect of calf death loss on cloned cattle herd derived from somatic cell nuclear transfer: Clones with congenital ... In cell biology, it is the propagation of a progenitor cell to obtain a population of genetically identical cells whereas, in ... Cultured somatic cells from a mare were used as nuclear donors in SCNT. Two of the resulting cloned embryos were then ...
https://www.pnas.org/content/112/29/8874?ijkey=049f3b1c3f32b40524c620b348237f176f822286&keytype2=tf_ipsecsha
*  Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres | PNAS
... the gray cell represents the most immature and the green cell is the most mature clonogenic cell. Three clone-forming cells are ... Next, we assumed that there is a limited number of neural clone-forming cell types, and each type of clone-forming cell is ... Stem cells tend to have longer cell cycles than progenitor cells (28), and the selective killing of more rapidly dividing cells ... clones were small. Culturing cells with EGF and FGF2 produce both types of clones (29). Given the fact that large clones of ...
https://www.pnas.org/content/99/22/14506?ijkey=f86eb9a52be15a576c2b04f382d6f1f73699b91e&keytype2=tf_ipsecsha
*  The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection | PNAS
1978) Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res 38:3751-3757. ... IFN Treatment of Cultured Cells.. CHME3 cells were seeded (2 × 105) in 6-well plates. The next day, cells were treated with ... Cells and Viruses.. Astrocytes, 1321N1 (23), microglia cells, CHME3 (24), and neurons, SH-SY5Y (22) have been previously ... Infection of Cultured Cells and Transduction Assays.. Target cells (1 × 105) were infected as described previously (14). HIV-1- ...
https://www.pnas.org/content/106/33/14040
*  The HLA-DQ2 gene dose effect in celiac disease is directly related to the magnitude and breadth of gluten-specific T cell...
1, and an overview for all T cell clones is given in Table 1. All gluten peptides stimulated the T cell clones in the presence ... Gene dose effect in stimulation of gluten-specific T cell clones. Stimulation of two gluten-specific T cell clones with the ... Next, T cell proliferation assays were performed with T cell clones specific for the gluten peptides presented by HLA-DQ2.5- or ... T cell proliferation experiments with the HLA-DQ2-transduced B cells were performed with 104 gluten-specific T cells stimulated ...
http://www.pnas.org/content/100/21/12390?ijkey=5b935b2102d925c44d4f10f8f7428349f829ece7&keytype2=tf_ipsecsha
*  Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts | PNAS
2003) Cloning animals by somatic cell nuclear transfer-Biological factors. Reprod Biol Endocrinol 1:98. ... inner cell mass. Under specific culture conditions embryonic stem cells (ESCs) can be captured and expanded from the inner cell ... 2007) Whole-genome analysis of histone H3 lysine 4 and lysine 27 methylation in human embryonic stem cells. Cell Stem Cell 1: ... 2014) The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification. ...
https://www.pnas.org/content/115/9/2090
*  Staphylococcus aureus targets the purine salvage pathway to kill phagocytes | PNAS
Sequencing results for mutated exon 2 alleles cloned from ADK−/− cells and mutated exon 4 alleles cloned from DCK−/− cells ( ... Cell grafts for epilepsy treatment. Human induced pluripotent stem cell-derived medial ganglionic eminence cells alleviate ... intoxication in U937 cells. (D) Analysis of cell viability for GeCKO library-transduced U937 cells intoxicated with 5 μM dAdo ... we incubated U937 cells with [3H]dAdo and measured uptake via scintillation analysis of cell lysates. U937 cells imported ...
https://www.pnas.org/content/115/26/6846
*  Fitness effects of advantageous mutations in evolving Escherichia coli populations | PNAS
Ten parallel cultures were started from a single cell carrying a cloned microsatellite. The cultures were propagated for about ... Cell grafts for epilepsy treatment. Human induced pluripotent stem cell-derived medial ganglionic eminence cells alleviate ... Bacterial density at transfer was ≈5 × 108 cells per ml. Cell numbers were obtained by titration. Samples from each population ... where N0 is the number of cells during transfer and N12h is the number of cells after 12 h of growth. Population genetic theory ...
https://www.pnas.org/content/98/3/1113
*  aging | PNAS
Glimpse of natural selection of long-lived T-cell clones in healthy life Baojun Zhang, Qingzhu Jia, Cheryl Bock, Gang Chen, ...
http://www.pnas.org/keyword/aging?page=4
*  Phantom tones and suppressive masking by active nonlinear oscillation of the hair-cell bundle | PNAS
2010) Coupling a sensory hair-cell bundle to cyber clones enhances nonlinear amplification. Proc Natl Acad Sci USA 107:8079- ... Two forms of motility by mechanosensory hair cells have been implicated in the active process (18). The hair cell can power ... Same cell and stimulus series as in Fig. 2. (B) Level of the cubic difference products as a function of the frequency ratio f2/ ... C) For n = 11 cells with f2/f1 = 1.08 - 1.15, the arithmetic-mean level of the two cubic difference products was divided by ...
http://www.pnas.org/content/109/21/E1344
*  Fitness effects of advantageous mutations in evolving Escherichia coli populations | PNAS
Ten parallel cultures were started from a single cell carrying a cloned microsatellite. The cultures were propagated for about ... Bacterial density at transfer was ≈5 × 108 cells per ml. Cell numbers were obtained by titration. Samples from each population ... where N0 is the number of cells during transfer and N12h is the number of cells after 12 h of growth. Population genetic theory ... the analyzed population carried more than a single cell type. Recently, it has been shown that more than a single cell lineage ...
http://www.pnas.org/content/98/3/1113
*  Derivation of naïve human embryonic stem cells | PNAS
Naïve hESCs Double More Rapidly and Clone More Efficiently Than Primed hESCs.. We measured both cloning efficiency and ... typified by mEpiSCs and human ES cells (hESCs). The challenge in naïve cell maintenance has been protecting cells from ... 2007) New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 448(7150):196-199. ... Naïve cells depend upon the distal OCT4 enhancer, whereas primed cells use the proximal enhancer (5, 6). We detected a similar ...
http://www.pnas.org/content/111/12/4484
*  Frizzled6 controls hair patterning in mice | PNAS
Embryonic stem cell clones carrying the homologously recombined allele were injected into C57BL6 blastocysts. Animal breeding ... Cell grafts for epilepsy treatment. Human induced pluripotent stem cell-derived medial ganglionic eminence cells alleviate ... 4E ). These observations suggest that even a small number of Fz6(+/+) cells adjacent to or interspersed with Fz6(-/-) cells can ... Linearized DNA was electroporated into 129 embryonic stem cells and the cells were grown in medium containing G418 and ...
https://www.pnas.org/content/101/25/9277
*  Submegabase copy number variations arise during cerebral cortical neurogenesis as revealed by single-cell whole-genome...
Our use of fetal mouse brain cells limited to a mean of ∼20 cells per brain likely missed low-frequency clones that were ... A) A B cell identified by deletions in Ig light and heavy chain regions. (B) A T cell identified by deletions in the T cell ... 2014) Single-cell, genome-wide sequencing identifies clonal somatic copy-number variation in the human brain. Cell Rep 8:1280- ... 2013) Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells. Nat Biotechnol 31 ...
http://www.pnas.org/content/115/42/10804
*  Bbs2-null mice have neurosensory deficits, a defect in social dominance, and retinopathy associated with mislocalization of...
... cells (129 × 1/SvJ3 129S1/Sv). G418-resistant clones were screened by PCR to identify Bbs2-targeted ES cell lines. Two ES cell ... Cell grafts for epilepsy treatment. Human induced pluripotent stem cell-derived medial ganglionic eminence cells alleviate ... 1), transfected the construct into ES cells, and identified five homologous recombinants by genotyping 1,056 ES cell lines (0.5 ... Dysfunction of Heterotrimeric Kinesin-2 in Rod Photoreceptor Cells and the Role of Opsin Mislocalization in Rapid Cell Death ...
https://www.pnas.org/content/101/47/16588
*  The genetics of geometry | PNAS
For vertebrate limb bud development, cells from clones can become intermixed with neighboring cells. However, even here cell ... This situation applies to plants where cell walls normally prevent cells from sliding relative to each other (5). (ii) Cell ... Thus, cells in the region labeled (1,1) at a later time need not be derived from cells of region (1,1) at an earlier time. ... 6, the concentration of tally molecule on the inner side of the cell membrane is raised at the top of the cell, where morphogen ...
https://www.pnas.org/content/101/14/4728
*  Table of Contents - December 23, 2003, 100 (26) | PNAS
Induction of colitis by a CD4+ T cell clone specific for a bacterial epitope Marika C. Kullberg, John F. Andersen, Peter L. ... Cell Biology. *. You have access. TGF-β-induced RhoA and p160ROCK activation is involved in the inhibition of Cdc25A with ... Antigen-driven effector CD8 T cell function regulated by T-bet Brandon M. Sullivan, Amy Juedes, Susanne J. Szabo, Matthias von ... Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death Wen-Hua Zhang, Xin Wang, ...
http://www.pnas.org/content/100/26/
*  Differential ability of B cells specific for external vs. internal influenza virus proteins to respond to help from influenza...
... enabling the B cells to interact with TH clones specific for any virion protein. B cells recognizing internal viral components ... internal influenza virus proteins to respond to help from influenza virus-specific T-cell clones in vivo. P A Scherle and W ... When a helper T-cell (TH) clone specific for the hemagglutinin, neuraminidase, matrix protein, or nucleoprotein of influenza ... internal influenza virus proteins to respond to help from influenza virus-specific T-cell clones in vivo ...
https://www.pnas.org/content/85/12/4446?ijkey=6be66e5213657f97bdc3a824bc20ac2a5e7cab2e&keytype2=tf_ipsecsha
*  TIR-only protein RBA1 recognizes a pathogen effector to regulate cell death in Arabidopsis | PNAS
Gateway-compatible Entry clones and Destination clones were generated by BP and LR cloning (Invitrogen) or by direct synthesis ... 2013) The TIKI/TraB/PrgY family: A common protease fold for cell signaling from bacteria to metazoa? Dev Cell 25(3):225-227. ... 2011) Programmed cell death in the plant immune system. Cell Death Differ 18(8):1247-1256. ... coli BL21(DE3) cells. Cells were grown in 1 L Terrific Broth (ampicillin 100 μg/mL, chloramphenicol 33 μg/mL) at 37 °C to an OD ...
http://www.pnas.org/content/114/10/E2053
*  clag9: A cytoadherence gene in Plasmodium falciparum essential for binding of parasitized erythrocytes to CD36 | PNAS
Clone B8, for example, can adhere to melanoma cells, although it is KAHRP-negative and knob-negative (12). Recently, a targeted ... The majority of the clones obtained were of the integrated genotype as determined by PCR analysis; two clones (11E and B1) were ... Ligands on the surface of parasitized red cells can bind to a number of endothelial cell receptors, including CD36 (1), ... Attempts to increase the level of binding of clone 11E to melanoma cells by up-selection four successive times failed and the ...
https://www.pnas.org/content/97/8/4029
*  Adaptive benefits from small mutation supplies in an antibiotic resistance enzyme | PNAS
Digested amplicons were then cloned into vector pACSE3 and transformed into competent DH5αE cells. We started the experiment by ... the selected clone was directly used as template for a new round of evolution; for SL lines, we continued with the clone from ... because sequencing 20 further clones from this population identified the selected mutation in only one case and the clone ... This lower initial cell density of SL lines allowed for more generations of selection, which could have affected the efficiency ...
https://www.pnas.org/content/114/48/12773?ijkey=bd70bd4b54dc2f10f64ea2e0599f89e2ad7499c5&keytype2=tf_ipsecsha
*  Adaptive benefits from small mutation supplies in an antibiotic resistance enzyme | PNAS
Digested amplicons were then cloned into vector pACSE3 and transformed into competent DH5αE cells. We started the experiment by ... Nondestructive sampling of cell contents. Nick Melosh describes a method for sampling RNA and proteins from cells using ... the selected clone was directly used as template for a new round of evolution; for SL lines, we continued with the clone from ... because sequencing 20 further clones from this population identified the selected mutation in only one case and the clone ...
http://www.pnas.org/content/114/48/12773
*  Somatic mtDNA mutations cause aging phenotypes without affecting reactive oxygen species production | PNAS
All cell lines were continuously cultured without cloning, and immortalized MEF cell lines were obtained. ... We also incubated cells with 10 mM H2O2 for 1 h (Fig. 2C ) and observed no difference in the fraction of apoptotic cells, ... which accumulates in necrotic cells. This approach allows assessment of cell viability by FACS analysis because live cells do ... Cells were permeabilized by using 1.1 μl of 5 mg/ml digitonin per 1 × 106 cells. Maximum uncoupled respiration rate was ...
http://www.pnas.org/content/102/50/17993