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*  Microbiology Society Journals | A Persistent and Inapparent Infection of L Cells with the Virus of Lymphocytic Choriomeningitis
Most clones from single cells carried lymphocytic choriomeningitis-specific antigen in every cell. A few clones were evidently ... After prolonged cultivation the carried virus was found to be incomplete; its ability to spread from cell to cell was greatly ... Serial cultivation of infected cells in a medium free of antibodies was easily accomplished. Initially, retardation of cell ... Some cultures derived from single cells consisted of a mixed population of infected and antigen-free cells. Homologous ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-5-1-63
*  Microbiology Society Journals | Generation of cytotoxic T-cell lines using overlapping pentadecapeptides derived from conserved...
The higher rate of infection in recipients of T cell-depleted grafts and in those undergoing T cell-targeted treatment during ... By focusing the response on the conserved sequences of hexon, the cell lines are likely to recognize most of the serotypes ... Of eight lines that were characterized extensively, seven included both CD4+ and CD8+ T cells and each recognized between two ... We have developed an approach to expand adenovirus-specific T cells using a pool of overlapping pentadecapeptides derived from ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.019471-0
*  Microbiology Society Journals | Vaccination with whole inactivated virus vaccine affects the induction of heterosubtypic...
The reduction in protective immunity correlated with changes in the immunodominance patterns of the CD8+ T-cell responses ... the magnitude of the CD8+ T-cell response to both peptides was similar on secondary infection with A/H5N1 influenza virus. In ... Taylor, P. M. & Askonas, B. A. ( 1986 ). Influenza nucleoprotein-specific cytotoxic T-cell clones are protective in vivo. ... NKT cells, or γδ T cells. J Immunol 166, 7437-7445.[CrossRef] ... Passive transfer of immune spleen cells to mice with influenza ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.020784-0
*  Microbiology Society Journals | A functional CD8+ cell assay reveals individual variation in CD8+ cell antiviral efficacy and...
It was demonstrated that CD8+ cells reduced the lifespan of infected CD4+ cells to 1 day, considerably shorter than the 30 day ... it was shown that HTLV-I-infected individuals vary considerably in the rate at which their CD8+ cells clear infected cells, and ... Experiments to ascertain the impact of CD8+ cells on viral burden based on CD8+ cell frequency or specificity alone give ... This novel approach demonstrates that CD8+ cells are a major determinant of HTLV-I proviral load. This assay is applicable to ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80766-0
*  Microbiology Society Journals | Evidence of Muller's ratchet in herpes simplex virus type 1
Two out of a total of 23 clones did not survive to the last transfer in 143 TK- cells. DNA from three genomic regions (DNA ... Four plaque-transferred populations lost neurovirulence for mice, as compared with the corresponding initial clones. LD50 ... values obtained with the populations subjected to serial bottlenecks were 4- to 67-fold higher than for their parental clones. ... Genetic and biological variation of herpes simplex virus type 1 (HSV-1) has been studied by subjecting 23 biological clones of ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.044685-0
*  Microbiology Society Journals | Functional interaction of Oct transcription factors with the family of repeats in Epstein-Barr...
This may have direct implications for B cell-specific regulation of Cp. ... could drive transcription of a heterologous thymidine kinase promoter linked to the FR in both B cells and epithelial cells. Cp ... A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells. Nature 336, 544 ... could drive transcription of a heterologous thymidine kinase promoter linked to the FR in both B cells and epithelial cells. Cp ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80620-0
*  Microbiology Society Journals | Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces...
... clone of HVT was constructed. DNA from two independent BAC clones, upon transfection into chicken embryo fibroblasts, produced ... Viruses derived from the BAC clones were stable during in vitro passage, but showed differences in in vitro growth kinetics ... In spite of the difference in in vitro growth, viruses from both clones induced 100 % protection against infection by the ... Furthermore, the availability of the BAC clones enables use of HVT as a vector for expressing foreign genes. ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81498-0
*  Microbiology Society Journals | Hepatitis C virus NS5A: tales of a promiscuous protein
Early studies into the effects of NS5A on cell physiology relied on expression of NS5A either alone or in the context of other ... The effects of NS5A on interferon signalling, and the regulation of cell growth and apoptosis are highlighted, demonstrating ... NS5A is a pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. ... from an infectious molecular clone of the HCV-N strain of hepatitis C virus replicate efficiently in cultured Huh7 cells. J ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80204-0
*  Microbiology Society Journals | Hepatitis C virus p7 protein is localized in the endoplasmic reticulum when it is encoded by a...
Following a few passages of the transfected cells, the recombinant genome with the HA tag reverted to wild-type and the entire ... showed that intracellular virus-like particles formed in the cells transfected with the wild-type, but not the recombinant, ... The tagged viral polyprotein was expressed transiently in the cells after transfection with the recombinant RNA transcripts. ... Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989 ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82049-0
*  Microbiology Society Journals | Construction and application of chimeric virus-like particles of tick-borne encephalitis virus...
The VLPs were able to enter only those cells that were derived from the natural vectors for the respective viruses. TBE virus ... but could neither replicate nor produce viral proteins in mosquito cells. These findings indicate the importance of specific ... Furthermore, the infectivities of the TBE virus- and JE virus-enveloped VLPs for the ISE6 tick cell line and C6/36 mosquito ... replicon RNA packaged in VLPs produced TBE virus non-structural proteins in tick cells, ...
https://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82824-0
*  Microbiology Society Journals | Supervirulent pseudorecombination and asymmetric synergism between genomic components of two...
Srivastava, K. M., Hallan, V., Raizada, R. K., Chandra, G., Singh, B. P. & Sane, P. V. ( 1995 ). Molecular cloning of Indian ... Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells. Proc Natl ... Stanley, J., Townsend, R. & Curson, S. J. ( 1985 ). Pseudorecombinants between cloned DNAs of two isolates of cassava latent ... Pseudorecombination between infectious cloned DNA components of tomato mottle and bean dwarf mosaic geminiviruses. J Gen Virol ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82873-0
*  Microbiology Society Journals | Cell-to-cell movement of potato virus X involves distinct functions of the coat protein
... which includes a number of overlapping functional domains important for cell-to-cell transport, provides an activity ... that the potyvirus CPs and both the major and minor CPs of beet yellows closterovirus could complement cell-to-cell movement of ... Sambrook, J., Fritsch, E. F. & Maniatis, T. A. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold ... Cell-to-cell and phloem-mediated transport of potato virus X. The role of virions. Plant Cell 10, 495-510.[CrossRef] [Google ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-2-449
*  Microbiology Society Journals | Simian varicella virus inhibits the interferon gamma signalling pathway
SVV infection did not downregulate IFNγ receptor α and β chain expression on the cell surface. Instead, STAT1, Janus tyrosine ... kinases 1 (JAK1) and JAK2 protein levels were significantly decreased in SVV-infected cells. Collectively, these results ... was blocked in SVV-infected cells, which did not involve cellular and viral phosphatases. ... Cloning the simian varicella virus genome in E. coli as an infectious bacterial artificial chromosome. Arch Virol 2011;156: 739 ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000925
*  Microbiology Society Journals | Avian virulence and thermostable replication of the North American strain of West Nile virus
... cells cultured at temperatures that were tolerated by the cell line. Unlike the NY99 virus, marked decreases in KEN-3829 viral ... in DEF cells incubated at 44 °C relative to levels of intracellular virus-specific RNA measured at 37 °C. In contrast, ... Viruses rescued from infectious cDNA clones of both the NY99 and KEN-3829 strains demonstrated virulence comparable to that of ... Shi, P.-Y., Tilgner, M., Lo, M. K., Kent, K. A. & Bernard, K. A. ( 2002 ). Infectious cDNA clone of the epidemic West Nile ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82299-0
*  Microbiology Society Journals | Defining of MAbs-neutralizing sites on the surface glycoproteins Gn and Gc of a hantavirus...
Rubinstein ND, Mayrose I, Halperin D, Yekutieli D, Gershoni JM et al. Computational characterization of B-cell epitopes. Mol ... Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and ... Mining for the antibody-antigen interacting associations that predict the B cell epitopes. BMC Struct Biol 2010;10 Suppl 1:S6 [ ... Structural analysis of B-cell epitopes in antibody:protein complexes. Mol Immunol 2013;53:24-34 [CrossRef][PubMed] ...
https://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001202
*  Microbiology Society Journals | Direct repeats in the 3′ untranslated regions of mosquito-borne flaviviruses: possible...
Blackwell, J. L. & Brinton, M. A. ( 1995 ). BHK cell proteins that bind to the 3′ stem-loop structure of the West Nile virus ... Pletnev, A. G. ( 2001 ). Infectious cDNA clone of attenuated Langat tick-borne flavivirus (strain E5) and a 3′ deletion mutant ... Gritsun, T. S. & Gould, E. A. ( 2006a ). The 3′ untranslated regions of Kamiti River virus and Cell fusing agent virus ... A stable full-length yellow fever virus cDNA clone and the role of conserved RNA elements in flavivirus replication. J Gen ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82235-0
*  Microbiology Society Journals | Chimeric pneumovirus nucleocapsid (N) proteins allow identification of amino acids essential...
Cytoplasmic inclusions of respiratory syncytial virus-infected cells: formation of inclusion bodies in transfected cells that ... Yu, Q., Hardy, R. W. & Wertz, G. W. ( 1995 ). Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, and L ... Meric, C., Spehner, D. & Mazarin, V. ( 1994 ). Respiratory syncytial virus nucleocapsid protein (N) expressed in insect cells ... Bovine respiratory syncytial virus nucleocapsid protein expressed in insect cells specifically interacts with the ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19370-0
*  Microbiology Society Journals | Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom...
We cloned and sequenced their RNA genomes. The sizes of the three genomic RNAs of OuMV, the type member of the genus, were 2814 ... Lee, J.-Y. & Lucas, W. J. ( 2001 ). Phosphorylation of viral movement proteins - regulation of cell-to-cell trafficking. Trends ... We cloned and sequenced their RNA genomes. The sizes of the three genomic RNAs of OuMV, the type member of the genus, were 2814 ... Höfgen, R. & Willmitzer, L. ( 1988 ). Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16, 9877 ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.013086-0
*  Microbiology Society Journals | Two isoforms of the protein kinase pUL97 of human cytomegalovirus are differentially regulated...
Kawaguchi, Y., Matsumura, T., Roizman, B. & Hirai, K. ( 1999 ). Cellular elongation factor 1δ is modified in cells infected ... Cloning and sequencing of a highly productive, endotheliotropic virus strain derived from human cytomegalovirus TB40/E. J Gen ... Kosugi, S., Hasebe, M., Tomita, M. & Yanagawa, H. ( 2009 ). Systematic identification of yeast cell cycle-dependent ... Phosphorylation of the RNA polymerase II carboxyl-terminal domain in human cytomegalovirus-infected cells and in vitro by the ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.026799-0
*  Microbiology Society Journals | Molecular characterization of M1146, an American isolate of Ljungan virus (LV) reveals the...
... primary 2A/2B cleavage in clone-derived precursors. Virology 190, 754-762.[CrossRef] ... three diverse picornaviruses are related to each other and to the H-rev107 family of proteins involved in the control of cell ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18792-0
*  Microbiology Society Journals | Marmosets (Callithrix jacchus) as a non-human primate model for evaluation of candidate dengue...
49. Angelo MA, Grifoni A, O'Rourke PH, Sidney J, Paul S et al. Human CD4+ T cell responses to an attenuated tetravalent dengue ... 5. Kinney RM, Butrapet S, Chang GJ, Tsuchiya KR, Roehrig JT et al. Construction of infectious cDNA clones for dengue 2 virus: ... Analysis of the cross-talk of Epstein-Barr virus-infected B cells with T cells in the marmoset. Clin Transl Immunology 2017;6: ... T-cell immunity to infection with dengue virus in humans. Front Immunol 2014;5: 93 [CrossRef] [PubMed] ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000913
*  Microbiology Society Journals | Comprehensive linker-scanning mutagenesis of the hepatitis C virus E1 and E2 envelope...
Helle F. , Dubuisson J. ( 2008 ). Hepatitis C virus entry into host cells. Cell Mol Life Sci 65, 100-112. [CrossRef] [PubMed] ... Yanagi M. , Purcell R. H. , Emerson S. U. , Bukh J. ( 1997 ). Transcripts from a single full-length cDNA clone of hepatitis C ... using a cell-cell fusion assay. J Virol 80, 1817-1825. [CrossRef] [PubMed] ... Burlone M. E. , Budkowska A. ( 2009 ). Hepatitis C virus cell entry: role of lipoproteins and cellular receptors. J Gen Virol ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.034314-0
*  Microbiology Society Journals | Isolation and full-length sequence analysis of Armigeres subalbatus totivirus, the first...
AsTV was shown to grow in both mosquito and mammalian cells, suggesting that it is an arbovirus that can infect mammals. ... AsTV was shown to grow in both mosquito and mammalian cells, suggesting that it is an arbovirus that can infect mammals. ... primary 2A/2B cleavage in clone-derived precursors. Virology 190, 754-762.. ... Genomic RNA of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.024794-0
*  Microbiology Society Journals | Genetic and antigenic diversity among noroviruses
... but expression of recombinant VP1 in insect cells results in the formation of virus-like particles (VLPs). In order to ... Human NoV strains cannot be propagated in cell-culture systems, ... Molecular cloning, expression, and antigenicity of Seto virus ... Human NoV strains cannot be propagated in cell-culture systems, but expression of recombinant VP1 in insect cells results in ... Jiang, X., Graham, D. Y., Wang, K. N. & Estes, M. K. ( 1990 ). Norwalk virus genome cloning and characterization. Science 250, ...
http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81532-0