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*  Frontiers | Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells | Immunology
HLA A*0201-restricted PPI-specific and HLA B*2705-restricted VIPR1-specific T-cell clones generated using the optimised ... specific T-cells but recent evidence shows that normal use of these reagents can miss fully functional T-cells that bear T-cell ... This issue is particularly pronounced for anti-cancer and autoimmune T-cells as self-reactive T-cell populations are enriched ... This issue is particularly pronounced for anti-cancer and autoimmune T-cells as self-reactive T-cell populations are enriched ...
https://www.frontiersin.org/articles/10.3389/fimmu.2018.01378/full
*  Frontiers | Autoreactive T Cells in Human Smokers is Predictive of Clinical Outcome | Immunology
We developed a dual cytokine enzyme-linked immunocell spot assay, the γ-6 Spot, using CD4 T cell IFN-γ and IL-6 responses and ... We developed a dual cytokine enzyme-linked immunocell spot assay, the γ-6 Spot, using CD4 T cell IFN-γ and IL-6 responses and ... Here we examined the utility of detecting autoreactive T cells as a screening tool for emphysema in an at-risk population of ... After adjusting for potential confounders, the presence of autoreactive T cells was predictive of a decrease in 6MWD over two ...
https://www.frontiersin.org/articles/10.3389/fimmu.2012.00267/full
*  Frontiers | Screening of an E. coli O157:H7 Bacterial Artificial Chromosome Library by Comparative Genomic Hybridization to...
... one involved three rounds of adherence assays to bovine primary rectal epithelial cells while the other competed the clones ... The library contains 1152 clones averaging 150 kbp. To verify the library, clones containing a complete locus of enterocyte ... one involved three rounds of adherence assays to bovine primary rectal epithelial cells while the other competed the clones ... However, conjugation of one of the BAC clones into a strain containing a partial LEE deletion restored type III secretion. 384 ...
https://www.frontiersin.org/articles/10.3389/fmicb.2011.00168/full
*  Frontiers | Characterization of a Suppressive Cis-acting Element in the Epstein-Barr Virus LMP1 Promoter | Microbiology
... the mutant exhibited increased LMP1 expression and EBV-mediated immortalization of B cells. However, single mutations of either ... the mutant exhibited increased LMP1 expression and EBV-mediated immortalization of B cells. However, single mutations of either ... and is essential for immortalization of B cells by the virus. Previous studies suggested that several transcription factors, ... and is essential for immortalization of B cells by the virus. Previous studies suggested that several transcription factors, ...
https://www.frontiersin.org/articles/10.3389/fmicb.2017.02302/full
*  Frontiers | The Different Functional Distribution of "Not Effector" T Cells (Treg/Tnull) in Colorectal Cancer | Immunology
... and the major players in tumoral immunity are effector T cells. Anyway, cancer cells develop strategies of immunoevasion ... Therefore, the T cell subsets that mature during the stages of tumor growth, differently contribute to disease progression and/ ... Therefore, the T cell subsets that mature during the stages of tumor growth, differently contribute to disease progression and/ ... We found that percentage of infiltrating effector T cells decreased in cancer tissue than in healthy mucosa and that the tumor ...
https://www.frontiersin.org/articles/10.3389/fimmu.2017.01900/full
*  Frontiers | CD4 Donor Lymphocyte Infusion Can Cause Conversion of Chimerism Without GVHD by Inducing Immune Responses Targeting...
Skin fibroblasts forced to express HLA class II, were recognized by only two MiHA specific CD4 T-cell clones. Of the 7 clones ... Skin fibroblasts forced to express HLA class II, were recognized by only two MiHA specific CD4 T-cell clones. Of the 7 clones ... Of the alloreactive T-cell clones, 96% were CD4 positive, illustrating the dominant role of CD4 T-cells in the immune responses ... Of the alloreactive T-cell clones, 96% were CD4 positive, illustrating the dominant role of CD4 T-cells in the immune responses ...
https://www.frontiersin.org/articles/10.3389/fimmu.2018.03016/full
*  Frontiers | Deletion of TGF-β1 Increases Bacterial Clearance by Cytotoxic T Cells in a Tuberculosis Granuloma Model | Immunology
We identify TGF-β1 as a major inhibitor of cytotoxic T-cell effector function in granulomas. Deletion of TGF-β1 from the system ... We also identify a novel dichotomous regulation of cytotoxic T cells and macrophages by TGF-β1 and IL-10, respectively. These ... We also identify a novel dichotomous regulation of cytotoxic T cells and macrophages by TGF-β1 and IL-10, respectively. These ... We identify TGF-β1 as a major inhibitor of cytotoxic T-cell effector function in granulomas. Deletion of TGF-β1 from the system ...
https://www.frontiersin.org/articles/10.3389/fimmu.2017.01843/full
*  Frontiers | Endoplasmic Reticulum Stress Response in Arabidopsis Roots | Plant Science
However, not all cells responded uniformly to the TM-induced ER stress in roots, as BiP3 was highly expressed in root tips, an ... However, not all cells responded uniformly to the TM-induced ER stress in roots, as BiP3 was highly expressed in root tips, an ... Karimi, M., De Meyer, B., and Hilson, P. (2005). Modular cloning in plant cells. Trends Plant Sci. 10, 103-105. doi: 10.1016/j. ... Nevertheless, the other cell did not show the mRFP signal at 24 h, suggesting that specific cells may respond to the ER stress ...
https://www.frontiersin.org/articles/10.3389/fpls.2017.00144/full
*  Frontiers | Orientia tsutsugamushi ankyrin repeat-containing protein family members are Type 1 secretion system substrates that...
This study identifies O. tsutsugamushi Anks as T1SS substrates and indicates that many display a tropism for the host cell ... and to where they localize in the host cell are not known. We determined that O. tsutsugamushi transcriptionally expresses 20 ... and to where they localize in the host cell are not known. We determined that O. tsutsugamushi transcriptionally expresses 20 ... that traffic to distinct subcellular locations and modulate host cell processes. The O. tsutsugamushi genome encodes one of the ...
https://www.frontiersin.org/articles/10.3389/fcimb.2014.00186/full
*  Frontiers | Genome Scan for Parent-of-Origin QTL Effects on Bovine Growth and Carcass Traits | Genetics
Expression levels of growth-regulating imprinted genes in cloned piglets. Cloning Stem Cells 9, 97-106. ... Trowbridge, J. J., and Orkin, S. H. (2010). DNA methylation in adult stem cells: new insights into self-renewal. Epigenetics 5 ... The DNA methylome of human peripheral blood mononuclear cells. PLoS Biol. 8, e1000533. doi: 10.1371/journal.pbio.1000533 ...
https://www.frontiersin.org/articles/10.3389/fgene.2011.00044/full
*  Frontiers | Inflammation and Elevation of Interleukin-12p40 in Patients with Schizophrenia | Frontiers in Molecular Neuroscience
Mosmann, T. R., Cherwinski, H., Bond, M. W., Giedlin, M. A., and Coffman, R. L. (1986). Two types of murine helper T cell clone ... Some have proposed that naïve T helper cells develop into type 1 T helper cells (Th1) or type 2 T helper cells (Th2) in ... Innate immunity is chiefly formed of circulating effector cells (mast cells, phagocytes, natural killer cells, and microglia) ... Cytokines are formed via outlying immunocompetent cells, glial cells, and neurons (Woodroofe, 1995; Na et al., 2014). It is ...
https://www.frontiersin.org/articles/10.3389/fnmol.2016.00016/full
*  Frontiers | Overexpression of Bovine FcRn in Mice Enhances T-Dependent Immune Responses by Amplifying T Helper Cell Frequency...
bFcRn expression in splenic B cells was also detected and that may also contribute to the enhanced B cell activation. Finally, ... bFcRn expression in splenic B cells was also detected and that may also contribute to the enhanced B cell activation. Finally, ... We also demonstrated that splenic dendritic cells of Tg mice express bFcRn and intraperitoneal immunization of these mice with ... We also demonstrated that splenic dendritic cells of Tg mice express bFcRn and intraperitoneal immunization of these mice with ...
https://www.frontiersin.org/articles/10.3389/fimmu.2015.00357/full
*  Frontiers | Inactive trans-Sialidase Expression in iTS-null Trypanosoma cruzi Generates Virulent Trypomastigotes | Cellular and...
We found that iTS expression improves parasite invasion of host cells and increases their in vivo virulence for mice as shown ... We found that iTS expression improves parasite invasion of host cells and increases their in vivo virulence for mice as shown ... of particular relevance due to its effect on the immune system and involvement in the interaction/invasion of the host cells. ... of particular relevance due to its effect on the immune system and involvement in the interaction/invasion of the host cells. ...
https://www.frontiersin.org/articles/10.3389/fcimb.2017.00430/full
*  Frontiers | Challenges in the Role of Gammaglobulin Replacement Therapy and Vaccination Strategies for Hematological Malignancy...
... or at B-cell malignancy diagnosis, when better antibody responses are attained. We have to re-emphasise the need for assessing ... or at B-cell malignancy diagnosis, when better antibody responses are attained. We have to re-emphasise the need for assessing ... Besides, patients affected by other B cell malignancies present with similar immunodeficiency and manifestations whilst they ... Besides, patients affected by other B cell malignancies present with similar immunodeficiency and manifestations whilst they ...
https://www.frontiersin.org/articles/10.3389/fimmu.2016.00317/full
*  Frontiers | N-Cadherin and Integrin Blockade Inhibit Arteriolar Myogenic Reactivity but not Pressure-Induced Increases in...
Thus, we tested the hypothesis that N-cadherin, a cell-cell adhesion molecule in vascular smooth muscle cells (VSMCs), was ... Thus, we tested the hypothesis that N-cadherin, a cell-cell adhesion molecule in vascular smooth muscle cells (VSMCs), was ... The possibility that a cell-cell adhesion receptor is involved has not been studied. ... The possibility that a cell-cell adhesion receptor is involved has not been studied. ...
https://www.frontiersin.org/articles/10.3389/fphys.2010.00165/full
*  Frontiers | Eight different types of dopaminergic neurons innervate the Drosophila mushroom body neuropil: anatomical and...
... expression and anti-TH immunoreactivity in the Drosophila protocerebrum and characterized single cell clones of the TH-GAL4 ... expression and anti-TH immunoreactivity in the Drosophila protocerebrum and characterized single cell clones of the TH-GAL4 ... dopamine, olfactory learning, Drosophila, single cell clones, functional optical imaging, tyrosine hydroxylase ... All anti-TH positive cells are also labeled by TH-GAL4. TH-GAL4 may express in one or two additional cells. Arrow: a cell that ...
https://www.frontiersin.org/articles/10.3389/neuro.04.005.2009/full
*  Frontiers | The confluence of radiotherapy and immunotherapy | Oncology
More recently, a specific receptor for necrotic cells was cloned and characterized. DNGR-1/CLEC9A, a c-type lectin, was shown ... stimulation of T cells through OX40 results in enhanced T cell activation and effector cell differentiation, in part, through ... by vascular endothelial cells within the tumor and induce expression of T cell chemokines that promote T cell adhesion and ... 2011). Host type I IFN signals are required for antitumor CD8+ T cell responses through CD8α+ dendritic cells. J. Exp. Med. 208 ...
https://www.frontiersin.org/articles/10.3389/fonc.2012.00143/full
*  Frontiers | Novel Antibody Drug Conjugates Targeting Tumor-Associated Receptor Tyrosine Kinase ROR2 by Functional Screening of...
Individual cellular 'Transpo-mAb' clones isolated by single cell sorting and capable of expressing membrane-bound as well as ... including renal cell carcinoma and osteosarcoma, making it an attractive target for targeted cancer therapy. Here, we describe ... and internalizing antibodies during the early phase of antibody discovery demonstrates the utility of the mammalian cell-based ... antibody discovery from immune libraries of human immunoglobulin transgenic animals using the Transpo-mAb mammalian cell-based ...
https://www.frontiersin.org/articles/10.3389/fimmu.2018.02490/full
*  Frontiers | Efficacy of a Virus-Like Nanoparticle As Treatment for a Chronic Viral Infection Is Hindered by IRAK1 Regulation...
Clone 13 (Cl13). Treatment of LCMV Cl13-infected mice with two systemic administrations of PapMV was ineffective, as shown by ... Clone 13 (Cl13). Treatment of LCMV Cl13-infected mice with two systemic administrations of PapMV was ineffective, as shown by ... Lymphocytic choriomeningitis virus clone 13 activates immune cells through the TLR7/MyD88 endosomal pathway (46) as well as the ... T cells (3, 4); and (iii) inducing differentiation of B cells into antibody secreting cells (5, 6). Thus early IFN-I production ...
https://www.frontiersin.org/articles/10.3389/fimmu.2017.01885/full
*  Frontiers | Laser Microdissection of the Alveolar Duct Enables Single-Cell Genomic Analysis | Oncology
3) The enzymatic and microfluidic isolation of single cells allowed for the harvest of as few as several thousand cells for PCR ... 3) The enzymatic and microfluidic isolation of single cells allowed for the harvest of as few as several thousand cells for PCR ... Here, we demonstrate an approach to conduct laser microdissection of the lung alveolar duct for single-cell PCR analysis. Our ... Microfluidics based arrays were used to measure the expression of selected marker genes in individual cells to characterize ...
https://www.frontiersin.org/articles/10.3389/fonc.2014.00260/full
*  Frontiers | Activated Mesenchymal Stromal Cells Process and Present Antigens Regulating Adaptive Immunity | Immunology
Conditioning of antigen-specific T-cells by activated and antigen-pulsed MSCs prevented T-cells to proliferate upon subsequent ... Conditioning of antigen-specific T-cells by activated and antigen-pulsed MSCs prevented T-cells to proliferate upon subsequent ... In sum, activation of MSCs with inflammatory stimuli turns these cells into suppressive cells, capable of mediating adaptive ... In sum, activation of MSCs with inflammatory stimuli turns these cells into suppressive cells, capable of med... ...
https://www.frontiersin.org/articles/10.3389/fimmu.2019.00694/full
*  Frontiers | Genome-Wide Identification of Dicer-Like, Argonaute, and RNA-Dependent RNA Polymerase Gene Families in Brassica...
1998). Isolation of an RNA-Directed RNA polymerase-specific cDNA clone from tomato. Plant Cell 10, 2087-2101. ... Cell. Microbiol. 6, 509-519. doi: 10.1111/j.1462-5822.2004.00399.x ...
https://www.frontiersin.org/articles/10.3389/fpls.2016.01614/full
*  Frontiers | How (specific) would you like your T-cells today? Generating T-cell therapeutic function through TCR-gene transfer ...
Their specificity is solely dictated by the T-cell receptor (TCR) they clonally express. As such, the genetic modification of T ... Their specificity is solely dictated by the T-cell receptor (TCR) they clonally express. As such, the genetic modification of T ... notable objective clinical responses were observed in terminally-ill cancer patients treated with TCR-gene modified cells in ... notable objective clinical responses were observed in terminally-ill cancer patients treated with TCR-gene modified cells in ...
https://www.frontiersin.org/articles/10.3389/fimmu.2012.00186/full
*  Frontiers | Root System Architecture and Abiotic Stress Tolerance: Current Knowledge in Root and Tuber Crops | Plant Science
2013). A novel protein kinase involved in Na(+) exclusion revealed from positional cloning. Plant Cell Environ. 36, 553-568. ... This cell cycle forms the LR founder cells that undergo several rounds of cell division to initiate LRs (Overvoorde et al., ... with rarely dividing cells that signals the surrounding cells to organize and maintain the initial stem cells (Dolan et al., ... Formation of LRs as an important aspect of RSA which is a result of cell division of a specific subset of pericyle cells ( ...
https://www.frontiersin.org/articles/10.3389/fpls.2016.01584/full
*  Frontiers | Urea Transporter Physiology Studied in Knockout Mice | Physiology
The UT-A transporter is mainly expressed in kidney epithelial cells; while UT-B demonstrates a broader distribution in kidney, ... The UT-A transporter is mainly expressed in kidney epithelial cells; while UT-B demonstrates a broader distribution in kidney, ... Cloning and functional expression of a urea transporter from human bone marrow cells. J. Biol. Chem. 269, 31649-31652. ... urea accumulates in the cells. Water enters the cells through AQP1 resulting in swelling and cell lysis. This could be measured ...
https://www.frontiersin.org/articles/10.3389/fphys.2012.00217/full