... and XRCC1 were from Cell Signaling; antibodies for BRCA1 were from Calbiochem (clone MS110); and antibodies for vinculin were ... Cell cultures, transfection, and immunoblotting. All cell lines were obtained from the American Type Culture Collection. Cells ... per each cell line. C, average of BRCA1 (left) and TGFBR1 (right) protein reduction in all cells analyzed after miRNA ... MCF7 cells (200 × 105 per 24-well plates) were cotransfected with 0.4 μg of pGL3 (luciferase), 0.08 μg of pRLTK (Renilla; ...http://cancerres.aacrjournals.org/content/70/7/2789
Molecular Imaging of Temporal Dynamics and Spatial Heterogeneity of Hypoxia-Inducible Factor-1 Signal Transduction Activity in...
... single cell-derived clones were obtained from selected reporter cell population. The clone #4C6-dxHRE-TKGFP-cmvRed2XPRT with ... The #4C6 reporter cells were seeded at 1.5 × 105 cells/dish in DMEM. Cells were cultured with or without the CoCl2 in the ... Selection of Transduced Tumor Cell Clones.. The C6-dxHRE-TKGFP-cmvRed2XPRT cell population was selected using fluorescence- ... Tumor Cells and Transduction.. The C6 rat glioma cell line was obtained from American Type Culture Collection (Manassas, VA). ...http://cancerres.aacrjournals.org/content/64/17/6101.long
Immunological Screening and Cloning of the Rat BARD1 cDNA.. A cDNA library of the PROb rat carcinoma cell line was constructed ... SW48 cells were arrested in G0 by contact inhibition in 175-cm 2 flasks. After 3 days of confluence, the cells were split 1:10 ... Cell Fractionation.. Cells were cultured in a 75-cm 2 flask, trypsinized, and resuspended in 100 μl of CEB buffer [50 mm HEPES ... 4 ⇓ , top). This was demonstrated by the addition of NaB 32 h after of cell plating, at which point 80% of the cells were in G0 ...http://cancerres.aacrjournals.org/content/60/24/6895
T cells and quantitative PCR-based T-cell repertoire analysis greatly improves the accuracy in detecting T-cell clones that ... β-gal-loaded 293Kb cells, the natural killer target YAC-1 cells, and B16 cells were admixed to hot B16 cells and tested in a ... C57BL/6 mice inoculated intravenously with B16LU8 cells received an inoculum of 5 × 106 cells of the different CTL clones 3 ... The 51Cr-labeled target cells (2,000 cells per well) were incubated with effector cells at various effector to target ratios in ...http://cancerres.aacrjournals.org/content/64/21/8068
Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586 | Cancer Research
Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586. Toby G. Rossman, Lawrence Prutkin and Victor R. Wheatley ... The cells are grown in a modified CMRL-1415 medium supplemented with 10% fetal calf serum. Clones tend to fall into two classes ... Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used ... Preliminary studies on the control of differentiation in Class 1 clones suggest that a minimum cell density is required before ...http://cancerres.aacrjournals.org/content/37/2/557
Synergy between Chemotherapy and Immunotherapy in the Treatment of Established Murine Solid Tumors | Cancer Research
3 The abbreviations used are: APC, antigen presenting cell; DC, dendritic cell; TCR, T-cell receptor; FACS, fluorescence- ... Clone 4 TCR transgenic mice (CL4; Ref. 23 ) express a TCR recognizing the dominant class I restricted epitope of influenza HA. ... tumorigenic cell line. AB1 cells were transfected with the influenza HA gene (AB1-HA; Ref. 26 ). Transfected cells were ... On neoplastic B cells, it can result in growth inhibition and cell death (20) . It has been shown in vivo that CD40 ligation ...http://cancerres.aacrjournals.org/content/63/15/4490?ijkey=7441150c91e33ec4ff8066e0c4af7d50e190e240&keytype2=tf_ipsecsha
Genome-Wide Impact of Androgen Receptor Trapped clone-27 Loss on Androgen-Regulated Transcription in Prostate Cancer Cells |...
Genome-Wide Impact of Androgen Receptor Trapped clone-27 Loss on Androgen-Regulated Transcription in Prostate Cancer Cells. ... Genome-Wide Impact of Androgen Receptor Trapped clone-27 Loss on Androgen-Regulated Transcription in Prostate Cancer Cells ... Genome-Wide Impact of Androgen Receptor Trapped clone-27 Loss on Androgen-Regulated Transcription in Prostate Cancer Cells ... Genome-Wide Impact of Androgen Receptor Trapped clone-27 Loss on Androgen-Regulated Transcription in Prostate Cancer Cells ...http://cancerres.aacrjournals.org/content/early/2009/03/24/0008-5472.CAN-08-3738.figures-only
Biosynthesis of an Interstitial Type of Collagen by Cloned Human Gastric Carcinoma Cells | Cancer Research
Biosynthesis of an Interstitial Type of Collagen by Cloned Human Gastric Carcinoma Cells. Kooko Sakakibara, Toshimitsu Suzuki, ... Biosynthesis of an Interstitial Type of Collagen by Cloned Human Gastric Carcinoma Cells ... Biosynthesis of an Interstitial Type of Collagen by Cloned Human Gastric Carcinoma Cells ... Biosynthesis of an Interstitial Type of Collagen by Cloned Human Gastric Carcinoma Cells ...http://cancerres.aacrjournals.org/content/42/5/2019
5-Azacytidine Induction of Stable Myoepithelial and Acinar Cells from a Human Salivary Intercalated Duct Cell Clone | Cancer...
A neoplastic human salivary intercalated duct cell clone was cultured in 5 µm 5-azacytidine for 5 days at 37°C; then the cells ... 5-Azacytidine Induction of Stable Myoepithelial and Acinar Cells from a Human Salivary Intercalated Duct Cell Clone. Mitsunobu ... 5-Azacytidine Induction of Stable Myoepithelial and Acinar Cells from a Human Salivary Intercalated Duct Cell Clone ... 5-Azacytidine Induction of Stable Myoepithelial and Acinar Cells from a Human Salivary Intercalated Duct Cell Clone ...http://cancerres.aacrjournals.org/content/47/16/4453
Overexpression of Kinesins Mediates Docetaxel Resistance in Breast Cancer Cells | Cancer Research
C, overexpression of KIFC3 in individual clones. D, cell survival assay with three individual clones, performed as in B. ... Generation of a VBIM-mutated cell library and selection of docetaxel resistant clones. MDA-MB 231 cells were plated at 10,000 ... Cells and reagents. MDA MB 231 cells and MDA MB 468 cells, kindly provided by Dr. John Pink (Case Western Reserve University, ... The cells were grown for 2 d and plated in six-well plates. When the cells attained 80% confluency, 300,000 cells were replated ...http://cancerres.aacrjournals.org/content/69/20/8035?ijkey=b501fb57c6e78593c41f5d789b75a516e1229e12&keytype2=tf_ipsecsha
An E2F-responsive Replication-selective Adenovirus Targeted to the Defective Cell Cycle in Cancer Cells | Cancer Research
1 ⇓ . After three rounds of plaque purification on 293 cells, one clone was processed for large-scale AdE2F-1RC preparation of ... nonproliferating cells (i.e., endothelial cells, epithelial cells, and fibroblasts), we reasoned that these cells would be the ... Cells were monitored for CPE. Complete CPE was observed in all of the tumor cells (Fig. 5B) ⇓ . In REN cells, a dose-dependent ... The cell killing effects of AdE2F-1RC and dl309 viruses on SKOV3, A549, and HeLa cells were comparable. Lowest cell-killing ...http://cancerres.aacrjournals.org/content/62/12/3438
Cell, Tumor, and Stem Cell Biology Molecular Cloning of hMena (ENAH) and Its Splice Variant hMena+11a: Epidermal Growth Factor ... activation and cell proliferation as evidenced in hMena+11a-transfected breast cancer cell lines. On the contrary, hMena ... 11a isoform is concomitantly present only in epithelial tumor cell lines. In breast cancer cell lines, epidermal growth factor ... Molecular Cloning of hMena (ENAH) and Its Splice Variant hMena+11a: Epidermal Growth Factor Increases Their Expression and ...http://cancerres.aacrjournals.org/content/67/6/2657
... a P1A-specific CTL clone did not lyse TS/A tumor cells but killed P1A peptide-pulsed 293Ld cells and J558 plasmacytoma cells ... Cell Lines and CTL Bulk Culture and Clone.. The TS/A parental cell line TS/A-pc (20) and its transfected derivatives TS/A- ... assays using peptide-loaded 293Ld cells and TS/A cells as cold cells to compete for lysis of 51Cr-labeled TS/A tumor cells. ... clone CTCD8α; Caltag, Burlingame, CA) and with hamster antimouse CD3-FITC (1 μg/106 cells; clone 145-2C11; Caltag). In vitro- ...http://cancerres.aacrjournals.org/content/63/9/2158
Perinatal or Adult Nf1 Inactivation Using Tamoxifen-Inducible PlpCre Each Cause Neurofibroma Formation | Cancer Research
Schwann cells with glutathione synthase (rabbit GS 1:250; Santa Cruz Biotech), mesenchymal cells (Sca-1 1:200; clone D7, BD- ... Those Schwann cells associated with neuronal cell bodies form satellite cells that express either S100β or GFAP. Schwann cells ... Tumors were composed of a heterogeneous hematopoietic cell population (B cells, T cells, and myeloid cells), which were EGFP ... perineurial cells, Schwann cells, and mast cells (7). Homozygous loss of NF1 is present only within the Schwann cell ...http://cancerres.aacrjournals.org/content/71/13/4675.long
Targeted Measles Virus Vector Displaying Echistatin Infects Endothelial Cells via αvβ3 and Leads to Tumor Regression | Cancer...
Cloning of p(+)MV-echistatin. The plasmid p(+)MV-echistatin was constructed stepwise. Step 1: SacII-PacI restriction fragment ... Infected cells were harvested then subjected to repeated freeze-thaw cycles to release cell associated viruses in PBS. Cell ... Unlike MV-Edm-infected cells, MV-ERV-infected cells roll outward from a central point where cells lose their attachment to the ... it is reasonable to hypothesize that even defective MV-ERV particles and cell membrane debris of infected cells can induce cell ...http://cancerres.aacrjournals.org/content/65/12/5292
Cell viability analysis revealed that knockdown of miR-214 alone reduced cell survival ∼20% in A2780CP cells. Moreover, ... Cloning and expression of miRNAs. Expression plasmids of miR-214 and miR-199a* were created by PCR amplification using human ... C, cell viability was detected by MTT assay. D, after 48 h of the treatment, cells were labeled with Annexin V and analyzed by ... A2780CP cells were transfected with 2′-O-me-anti-miR-214. The cells transfected with 2′-O-me-scrambled miR were used as control ...http://cancerres.aacrjournals.org/content/68/2/425
NORE1B Is a Putative Tumor Suppressor in Hepatocarcinogenesis and May Act via RASSF1A | Cancer Research
Cell transformation and cloning efficiency. Primary rat embryo cells (REC) were isolated at day 15.5 of gestation by ... B, cells were seeded at 4 × 105 per plate. Subconfluent cultures were harvested after 72 h and cells were counted. C, cell ... 4 ). Unlike the control clones with a fibroblastoid morphology and 0.4 cell doublings per day, NORE1Bwt-clones showed an ... The t(1;3) breakpoint-spanning genes LSAMP and NORE1 are involved in clear cell renal cell carcinomas. Cancer Cell 2003; 4: 405 ...http://cancerres.aacrjournals.org/content/69/1/235.long
Although NSCLC cells display a more complicated relationship between EGFR and MET than 32D cells, NSCLC cells similarly show ... Cloning. Site-directed mutagenesis was conducted with QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies) ... 6D). In contrast with other NCSLC cells, MET was not required for EGF-induced cell invasion of parental PC9 cells and knockdown ... MET promotes EGF-induced cell migration and invasion of NSCLC cell lines. NSCLC cell lines were pretreated for 3 hours with 10 ...http://cancerres.aacrjournals.org/content/73/16/5053
... and GVHD-associated T-cell clones. T-cell clones associated with GVM were detectable by clone-specific PCR at a low level in ... Similarly, 86 Vβ13.1+ clones were tested, and 27 clones were positive for the clone-specific CDR3 sequence. Selected clones ... T-cell clones associated with GVHD were not detectable before DLI or before the development of clinical GVHD. Two T-cell clones ... The cell surface phenotype of the p2-3 and p2-13.1 clones was also examined by flow cytometry. Both clones were CD3+CD8+CD4− T ...http://cancerres.aacrjournals.org/content/63/10/2561
Cells were treated with PIF alone (Lanes 1 through 3) or with PIF in the presence of 50 μmol/L EPA (Lanes 4 through 6), 50 μmol ... Coshocton, OH). Mouse monoclonal antibodies to 20S proteasome subunits α1, 2, 3, 5, 6, and 7 (clone MCP231), 20S proteasome ... Cells then were preincubated for 2 hours with and without EPA or HMB and then for 24 hours with PIF in fresh DMEM without ... Cell Culture.. C2C12 myotubes were routinely passaged in DMEM supplemented with 10% fetal calf serum, glutamine, and 1% ...http://cancerres.aacrjournals.org/content/64/23/8731
Primary Cilium Formation Requires von Hippel-Lindau Gene Function in Renal-Derived Cells | Cancer Research
... clone M-A261 (Serotec). Cells used for cell lysis were grown in parallel cultures under the same conditions as cells fixed for ... VHL induces renal cell differentiation and growth arrest through integration of cell-cell and cell-extracellular matrix ... cells. In vitro observations showed the importance of cell-cell and cell-extracellular matrix interactions in VHL-dependent ... Arrows, apical cell staining; arrowheads, lateral cell-cell staining. C, Western blot of CD26 and β-Catenin proteins from RCC( ...http://cancerres.aacrjournals.org/content/66/14/6903
Transcriptional Programs following Genetic Alterations in p53, INK4A, and H-Ras Genes along Defined Stages of Malignant...
Nuclei were stained with 4′,6-diamidino-2-phenylindole to show cell number in both control and TGF-β1-treated cells. Cells were ... The following primary antibodies were used: mouse monoclonal anti-α-smooth muscle actin (α-SMA; clone 1A4, Sigma) and mouse ... Tumor1 cells were obtained from a tumor formed by Tfast/R/G cells on injection into nude mice. Gene expression was normalized ... Tumor1 cells were recovered from a tumor formed by Tfast/R/G cells following injection into nude mice. Gene expression was ...http://cancerres.aacrjournals.org/content/65/11/4530?ijkey=8c42c55d29ea20e507c78ed367bf397a64342673&keytype2=tf_ipsecsha
Sunitinib Inhibition of Stat3 Induces Renal Cell Carcinoma Tumor Cell Apoptosis and Reduces Immunosuppressive Cells | Cancer...
... clone HE12) was from BD Biosciences; anti-cyclin D1 (AB-3) and anti-Bcl-2 were from Calbiochem; anti-β-actin (AC-74) was from ... Percentage of dead cells was calculated by dividing the number of trypan blue-stained cells by the total cell number (n = 3, in ... Top, viable cells represented by Annexin V-APC and DAPI double-negative cells were determined by flow cytometry; bottom, cell ... Sunitinib Inhibition of Stat3 Induces Renal Cell Carcinoma Tumor Cell Apoptosis and Reduces Immunosuppressive Cells. Hong Xin, ...http://cancerres.aacrjournals.org/content/69/6/2506
Heterogeneity in Induced Heat Resistance and Its Relation to Synthesis of Stress Proteins in Rat Tumor Cell Clones. Stephen P. ... Tumor cell clones isolated from a rat 13762NF mammary adenocarcinoma and its spontaneous metastases were heterogeneous in their ... Heterogeneity in Induced Heat Resistance and Its Relation to Synthesis of Stress Proteins in Rat Tumor Cell Clones ... Heterogeneity in Induced Heat Resistance and Its Relation to Synthesis of Stress Proteins in Rat Tumor Cell Clones ...http://cancerres.aacrjournals.org/content/44/12_Part_1/5850
Increased Hsp27 after Androgen Ablation Facilitates Androgen-Independent Progression in Prostate Cancer via Signal Transducers...
Treatment of cells with antisense oligonucleotides or short-interfering RNA. Cells were plated at a density of 30,000 cells by ... Two vectors were created for study: pHR′-CMV-Hsp27 and pHR′-CMV (empty vector). Clone identity was verified using restriction ... in LNCaP-Hsp27 cells than the LNCaP-empty control cells ( Fig. 3B). LNCaP-Hsp27 cells were also more resistant to paclitaxel- ... Tumor cell lines. The human prostatic cancer cell line LNCaP was kindly provided by Dr. Leland W.K. Chung (Emery University, ...http://cancerres.aacrjournals.org/content/65/23/11083?ijkey=742f36439dc7b7c50f11844a7ac7f831ad17e90d&keytype2=tf_ipsecsha