Loading...
*  Wanted - T cell clones, rheumatoid arthritis
... Mr. D.N.C. Hunter dhunter at hgmp.mrc.ac.uk Tue Sep 9 09:24:57 EST 1997 *Previous ... I don't have much experience in T cell cloning and I am led to believe that it is very time consuming and not always successful ... Ideally I would like to use human T cell clones derived from subjects with rheumatoid arthritis. ... I am presently looking at cytokine mediated differential gene expression in T cells. ...
http://www.bio.net/bionet/mm/immuno/1997-September/010957.html
*  TA cloning
Previous message: TA cloning *Next message: Transfection of suspension cells * Messages sorted by: [ date ] [ thread ] [ ... I tried once, it didn't work, and my generous boss told me to buy the , TA-cloning kits. I have used pGEM-T Easy, pCR-II and ... I have , tried this way of cloning blunt products by: , 1. Assuming Taq adds on an extra A tail in the PCR reaction and using a ... TA cloning. malbrech at asmoday-atm.rz.uni-frankfurt.de malbrech at asmoday-atm.rz.uni-frankfurt.de Thu Aug 20 04:22:48 EST ...
http://www.bio.net/bionet/mm/methods/1998-August/069640.html
*  looking for gene clone review
Previous message: COS cells/MMT I promoter *Next message: Semi-quantitative RT-PCR control ... looking for gene clone review. huang zhong zhong.huang at student.kuleuven.ac.be Wed Jul 9 18:06:27 EST 1997 * ... Dear netters, Can anyone here tell me some recently published reviews on gene cloning technique? Thanks ...
http://www.bio.net/bionet/mm/methods/1997-July/059211.html
*  cloning blunt
Quantitate the competency of your competent cells using supercoiled DNA; I suggest the uncut vector. In our experience, you ... cloning blunt. Steve Hartson shartson at BMB-FS1.BIOCHEM.OKSTATE.EDU Wed Nov 15 14:05:07 EST 1995 *Previous message: PC DNA ... Nandy Hofland (nandy at ruly46.medfac.leidenuniv.nl) wrote: For two months now I have been trying to clone a 2 kb blunt ... For forced-directional sticky-end cloning, I get a pseudo-lawn of transformants containing insert. You may also wish to ...
http://www.bio.net/bionet/mm/methods/1995-November/036336.html
*  Overexpression of a protien (fwd)
The insert was cloned into the NdeI site of the vector. Then,the ,the plasmid was tramsformed into BL21(DE3) cells. The clone ... The gene (2kb) was ,successfully cloned into pET21-a(+) vector. The cloned gene was excised ,by the same two enzymes (Nde I and ... The conditions for grow the cells is as follows: LB ,medium containing 100ug/ml ampicillin, when O. D. reaches 0.4-0.6, 100mM , ... It then depends on the toxicity of the product and/or the burden of the system on the cells what your outcome will be. ...
http://www.bio.net/bionet/mm/methods/1995-November/036119.html
*  Probe Query
Next message: SUMMARY: Cloning HL60 cells & Southern problems * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] ... We are wanting to express or detect in rat cells. *Previous message: Receptor Antibody Query ...
http://www.bio.net/bionet/mm/methods/1990-August/002494.html
*  Growing Up TA Cloning Products
For those of you that use the TA cloning kit (Invitrogen), : what is your prefered method for overnight cultures of your cells ... Growing Up TA Cloning Products. Lisa J Brunet brunet at uclink2.berkeley.edu Sun Aug 28 11:32:38 EST 1994 *Previous message: ...
http://www.bio.net/bionet/mm/methods/1994-August/017794.html
*  cloning confusion - HELP!!
When plating out competent ,cells on amp+ and amp- plates, we get growth on the amp- plates but no ,growth on the amp+ plates ... cloning confusion - HELP!!. John Nash num208jn at MBDS.NRC.CA Tue Nov 19 22:21:09 EST 1991 *Previous message: cloning confusion ...
http://www.bio.net/bionet/mm/methods/1991-November/002867.html
*  Cloning of murine antibodies
Hi Giorgio, in most B-cells (and hybridomas) Ig genes are rearranged on both alleles. Additional advantage is that by making ... Cloning of murine antibodies. ANDREI POPOV ANDREI.POPOV at afrc.ac.uk Sun Feb 12 14:31:00 EST 1995 *Previous message: CLONING ...
http://www.bio.net/bionet/mm/methods/1995-February/024414.html
*  CellNEWS - UK's First Human ES Cell Line Generated
Severino Antinori last week in Spain said that he soon will publish a detailed report of the progress of a human clone ... CellNEWS - UK's First Human ES Cell Line Generated Editor giantfideli at yahoo.co.uk Mon Aug 18 08:59:39 EST 2003 *Previous ... Remaining embryos, which may carry disease-causing genes, were used for the generation of the stem cell lines. CAMR Urges Bush ... The current policy, which allows federal funds to be used only on stem cell lines derived before August 9, 2001, has hindered ...
http://www.bio.net/bionet/mm/bionews/2003-August/005721.html
*  optimize mammalial cloning
I think the cells are dividing over the time and are so not longer be monoclonal. *Previous message: NH4Ac/EtOH ppt of DNA from ... optimize mammalial cloning. Uwe Uwe.Mahr at RODGAU.NETSURF.DE Thu Mar 7 19:22:42 EST 1996 *Previous message: NH4Ac/EtOH ppt of ... I think if i got a monoclonal mammalial cell-line which is overexpressing a receptor gene, it could improve the expression of ... is it possible to optimize a transfected cell-line (by G418-selection) by subcloning, i. e. to get a better receptor expession ...
http://www.bio.net/bionet/mm/methods/1996-March/041488.html
*  Following up baffling cloning results
In addition they carry the mutation that will stablize inverted repeats (a la SURE cells). Regards. *Previous message: DNA from ... Following up baffling cloning results. Karthik Aghoram kaghoram at unity.ncsu.edu Wed Aug 29 10:46:57 EST 2001 *Previous ... Thanks to all who responded! FYI: I found that STBL-2 cells from Invitrogen/Gibco (absolutely no affiliation whatsoever) are ...
http://www.bio.net/bionet/mm/methods/2001-August/090264.html
*  A unique retrovirus?
Gallo said in 1983 that he had discovered HTLV-I sequences in the ,HUT78 cell line. The H9 cell line is a clone of HUT78. ,( ... into a cell one gets out the SAME particle with the SAME ,constituents. Now try and find that evidence. , Found it! ,(end of ...
http://www.bio.net/bionet/mm/virology/1995-June/003491.html
*  invitro B-cell stimulation help!
I might be , , using the wrong clone or origin of the stimulating agents. I'm working , , with peripheral blood of cynomolgus ... Even better if you stimulate your , cells with IL-4 and anti-IgM. , , cheers , , david , , In article ,382A22EC.D1A9E24F at ... LPS should have worked but its not specific to B , cells. , , As for monkey anti-IgM ( anti-mu should be better), , try the ... Interestingly, this type of stimulation is T cell independent, although it prefferentially activates naive B cells. IdGrad ...
http://www.bio.net/bionet/mm/immuno/1999-December/015174.html
*  stable cell lines
... Muralikrishna Vemula vemula at helix.mgh.harvard.edu Thu Mar 7 14:00:42 EST 2002 *Previous message: Cloning ... Hello, I am working with HepG2-C3A hepatocyte cell line. My goal is to transfect these cells with GFP reporter plasmids and ... Do the cells usually have a different generation time after stable transfection? 3. Do you have to screen for clones that ... describes the procedure starting from trancfecting cells to performing gene expression studies using stably transfected cells? ...
http://www.bio.net/bionet/mm/methods/2002-March/092483.html
*  cloning a gene that has secondary structure
... lethal to the host cell due to depletion of resources (think about it--200 copies , of what would be approx. 40 kDa is the ... cloning a gene that has secondary structure. January Weiner nospam_jweiner1 at ix.urz.uni-heidelberg.de Fri Sep 25 10:56:12 EST ...
http://www.bio.net/bionet/mm/methods/1998-September/070572.html
*  Course announcement
... cDNA cloning; Week 3 - cell culture, gene transfer techniques, mutagenesis; Week 4 - basic bacteriology, characterization of ...
http://www.bio.net/bionet/mm/bionews/1993-April/000457.html
*  How large can be cloned into pUC19 ?
Sometimes I get a good yield of cells but a poor yield of plasmid. The strains we usually use are DH5alpha and XL1Blue. ... Linchengtao wrote: , Hi,netters: , Wish a good new year to everybody! , And is there anyone who have the experiences of cloning ... How large can be cloned into pUC19 ?. Phillip San Miguel pmiguel at bilbo.bio.purdue.edu Tue Dec 30 12:05:17 EST 1997 *Previous ... large , fragment into pUC19 ? I wonder the biggest size that can be cloned into pUC19 but not quite influence pUC19's high copy ...
http://www.bio.net/bionet/mm/methods/1997-December/063583.html
*  Postdoctoral Fellow/Research Associate Position
Applicants should be experienced with molecular cloning, cell culturing, and FACS sorting methods. Experience with subtractive ... 1998 to investigate mechanisms underlying the regulation of alternative splicing in mammalian cells. The project will focus on ...
http://www.bio.net/bionet/mm/yeast/1998-June/008017.html
*  Positions open.
A background in the development of engineered mammalian cell lines for pharmaceutical screening is desirable. RECEPTOR CLONING ... A background in expression cloning, subtractive hybridization, cell sorting or neuroscience would be advantageous. Interested ... Design and utilization of a variety of vector systems and host cells is preferred. ... Experience in cDNA cloning and a strong background in recombinant DNA technology is required. Preference will be given to ...
http://www.bio.net/bionet/mm/bioforum/1990-May/002540.html
*  expression in mammalian cells
I wish to express a clone of interest in mammalian cell lines. However, I want to be able to select for the transfected cells ... expression in mammalian cells. Fiberman wsun at jeeves.ucsd.edu Wed Oct 14 21:03:30 EST 1992 *Previous message: PCR cyclers ... Which plasmids are good for this purpose? I have HeLa, COS, and CHO cells. Any suggestions are welcome. -fm *Previous message: ... so I can maintain the plasmid in the cell line indefinitely. ...
http://www.bio.net/bionet/mm/methods/1992-October/003207.html
*  Question on basic cloning theory
Hi Can anyone tell me: Is there only one plasmid goes into single competent cell? If yes, why? why not 2 or more plasmids? If ... Question on basic cloning theory. rocky rockyho at hotmail.com Tue May 1 10:01:10 EST 2001 *Previous message: Problem with two ... no, how can I make sure I get a unique clone from the single bacterial colony? ( Note: I carry out an PCR for the target using ...
http://www.bio.net/bionet/mm/methods/2001-May/088641.html
*  Drosophila cell culture
I would really appreciate any references on the original cloning of these ,cell lines (my lit searches have turned up nothing ... Does anybody know the origins of the Schneider cell and Kc cell lines? , ... Drosophila cell culture. Mark D. Garfinkel garfinkl at iitmax.iit.edu Tue Feb 15 10:30:39 EST 1994 *Previous message: ... he gives in-text citation of the original papers describing the origins of the cell lines, on pages 133-138 he gives the ...
http://www.bio.net/bionet/mm/dros/1994-February/000277.html
*  Help : problem with bicistronic vector
Hello, I have cloned into the pIRES-puro vector from Clontech a cDNA and I have tranfected cells with it. I have obtained ... stable clones (selection with puro). The problem is : when I prepare the mRNA of these cells, and that I make a dot-blot, I ... moreover, when I use a probe 'puro' with the mRNA of my stable clones, I have a very important signal !!!! Help, any ...
http://www.bio.net/bionet/mm/cellbiol/1998-December/010100.html
*  alpha2,3-sialyltransferase anyone..?
... purified from CHO cell? Also is there's anybody who knows about cloning works on the same enzyme (reference or commercially ...
http://www.bio.net/bionet/mm/glycosci/1995-October/000381.html