Clathrin-mediated endocytosis is the process by which cargo-containing clathrin-coated vesicles bud off from the plasma ... Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. Science. 291: ... Although the budding of clathrin-coated vesicles in vitro has recently been shown to only require the presence of clathrin, an ... However, these studies on PIP2 depletion reported different effects on clathrin assembly. In one study the number of clathrin ...
Clathrin-coated vesicles are the most prominent carriers of membrane traffic from cell surface to endosomes (endocytosis), a ... technological and analytical advances that I developed to directly visualize clathrin-mediated membrane traffic in three ... using these advances that defined a role for actin filament polymerization in counteracting membrane tension during clathrin- ...
In this study, the genes ZmCHC1 and ZmCHC2 encoding clathrin heavy chain in maize were cloned and characterized for the first ... a three-legged triskelion composed of three clathrin heavy chains (CHCs) and three light chains (CLCs), plays a critical role ... in clathrin-mediated endocytosis (CME) in eukaryotic cells. ... Clathrin, a three-legged triskelion composed of three clathrin ... Clathrin is a protein that plays a major role in the formation of coated vesicles. Clathrin was first isolated and named by ...
CB, clathrin-binding site; CB-I and CB-II: clathrin-binding enhancing sites I and II, respectively; EBD, endofin-binding domain ... Clathrin-binding assay. The clathrin-binding assay with GST fusion protein was adapted from the protocol described in a ... Optimal clathrin binding by TOM1 involves three sites: residues 300-321, 321-326 and a putative clathrin-binding box at ... The polyclonal anti-clathrin heavy chain antibody for western blotting and monoclonal anti-clathrin heavy chain for ...
Clathrin heavy chain (Chc) co-immunopurifies with Bic-D in embryos and ovaries, and a fraction of Chc colocalizes with Bic-D. ... Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D ... Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D ... Clathrin heavy chain plays multiple roles in polarizing the Drosophila oocyte downstream of Bic-D ...
In animal cells, dynamin and clathrin play central roles in vesicle formation in the process of endocytosis, but the roles of ... The localization pattern and behavior of the puncta were similar to those of animal dynamin and clathrin signals reported ... Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits MERRIFIELD CJ ... Functional evidence for the identification of an Arabidopsis clathrin light chain polypeptide SCHEELE U ...
Assembly polypeptides from coated vesicles mediate reassembly of unique clathrin coats. Clathrin assembly protein AP-2 induces ... Here we show that the AP-2 adaptor, a clathrin-associated coat-protein complex that nucleates clathrin-coated vesicle formation ... AP-2-containing clathrin coats assemble on mature lysosomes. L M Traub, L M Traub ... L M Traub, S I Bannykh, J E Rodel, M Aridor, W E Balch, S Kornfeld; AP-2-containing clathrin coats assemble on mature lysosomes ...
J. Z. Rappoport and S. M. Simon, "Real-time analysis of clathrin-mediated endocytosis during cell migration," J. Cell Sci. 116( ... J. Z. Rappoport and S. M. Simon, "Real-time analysis of clathrin-mediated endocytosis during cell migration," J. Cell Sci. 116( ... J. Z. Rappoport and S. M. Simon, "Real-time analysis of clathrin-mediated endocytosis during cell migration," J. Cell Sci. 116( ... J. Z. Rappoport and S. M. Simon, "Real-time analysis of clathrin-mediated endocytosis during cell migration," J. Cell Sci. 116( ...
POB1 cellular colocalization with clathrin is consistent with this interaction, since Amphiphysin II is also a clathrin ligand ... The interaction of POB1 with Amphiphysin II is consistent with the co-localization of POB1 with clathrin, since Amphiphysin II ... Brett TJ, Traub LM, Fremont DH: Accessory protein recruitment motifs in clathrin-mediated endocytosis. Structure. 2002, 10 (6 ... anti-clathrin CHC (staining coated pits), anti-EEA1 (early endosomes), anti-CD63 (late endosomes), anti-GM130 (Golgi), and anti ...
Konopka, C. A., Backues, S. K. and Bednarek, S. Y. (2008). Dynamics of Arabidopsis Dynamin-Related Protein 1C and a Clathrin ...
Endocytosis of the low density lipoprotein (LDL) receptor (LDLR) in coated pits employs the clathrin adaptor protein ARH. ... Similarly, agonist-dependent endocytosis of heptahelical receptors in coated pits employs the clathrin adaptor beta-arrestin ...
Clathrin is a protein that plays a major role in the formation of coated vesicles. Clathrin was first isolated and named by ... This is how clathrin gets its name, from the Latin clathratus meaning like a lattice. Coat-proteins, like clathrin, are used to ... During mitosis, clathrin binds to the spindle apparatus, in complex with two other proteins: TACC3 and ch-TOG/CKAP5. Clathrin ... Model of Clathrin assembly). *. Pérez-Gómez J, Moore I (March 2007). "Plant endocytosis: it is clathrin after all". Current ...
Endocytosis without clathrin coats.. Nichols BJ1, Lippincott-Schwartz J.. Author information. 1. MRC Laboratory of Molecular ... Many markers for clathrin-independent endocytosis are found in detergent-resistant membrane fractions, or lipid rafts. We will ... Various non-clathrin endocytic mechanisms have been identified, including uptake through caveolae, macropinosomes and via a ... To date, most studies on endocytosis in mammalian cells have focused on pathways that start with uptake through clathrin-coated ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
Clathrin is a large heterohexameric protein complex composed of three heavy chains and three light chains. Clathrin molecules ... Clathrin-associated adaptor protein (AP) complexes are a stoichiometric coat component of CCVs alongside clathrin itself, and ... The connection of the clathrin scaffold to the membrane is mediated by clathrin adaptors, which can bind directly to both the ... clathrin. Clathrin assembles from three-legged individual components called triskelions to form a polygonal lattice around the ...
Aftiphilin contains a clathrin box, with two identified clathrin-binding motifs [PMID: 15811338, PMID: 15758025]. ... Aftiphilin is a component of the clathrin machinery in neurons.. FEBS Lett. 579 2177-84 2005 ...
Clathrin-mediated endocytosis persists during unperturbed mitosis.. Tacheva-Grigorova SK1, Santos AJ, Boucrot E, Kirchhausen T. ... The dynamics of clathrin-coated pit formation and the uptake of transferrin are maintained in naturally dividing cells but are ... B) Surface density of clathrin/AP2-coated structures (CCS/100 µm2) calculated from the number of AP2 spots at the top and ... C) Total number of clathrin/AP2-coated structures (CCS per cell) on the plasma membrane of a cell as determined by counting the ...
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Immunogen = synthetic peptide: E E D P A A A F L A Q Q E S E I A G I E N D, corresp. to amino acids 23-44 of Cow Clathrin light chain. ...
Vassilopoulos et al. (see the Perspective by Orme and Bogan) now describe a function for CHC22 clathrin, a second isoform of ... A role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. Science 324, 1192-1196 (2009). [Abstract] [Full ... clathrin that is present in humans and not in mice. CHC22 participates in the biogenesis of the intracellular compartment that ...
This binding then stimulates clathrin lattice assembly. HIP1 is an obligate binding partner for Huntingtin, and loss of this ... Huntingtin-interacting protein 1, clathrin-binding domain (IPR032422). Short name: HIP1_clath-bd ... Clathrin light-chain binds to a flexible coiled-coil domain in HIP1 and induces a compact state that is refractory to actin ... It carries a highly conserved HADLLRKN sequence motif at its N terminus which effects the binding of HIP1R to clathrin light- ...
Very few clathrin coats continue to assemble after AP-2 knockdown. Moreover, there is a total absence of clathrin-containing ... Conclusions/Significance These results suggest that AP-2 is essential for endocytic clathrin coated-pit and coated-vesicle ... They also indicate that under normal conditions, functional endocytic clathrin coated pits are required for LDL internalization ... the formation of clathrin-coated structures at the plasma membranes of BSC1 and HeLa cells depleted by RNAi of the clathrin ...
Clathrin-independent carriers (CLICs) are prevalent tubulovesicular membranes responsible for non-clathrin mediated endocytic ...
They found that clathrin takes over from adaptors as a hub as clathrin assembles into a coat. This shift requires collaboration ... Sharing Responsibility for Clathrin Coat Assembly. Aug 16, 2006 - 8:58:00 AM, Reviewed by: Dr. Priya Saxena. ... Clathrin coat formation, Schmid et al. propose, is an outgrowth of increasingly stable interactions among a shifting network of ... Clathrin molecules three-pronged pinwheel-shaped proteins form an elaborate lattice coat around the vesicles, which ultimately ...
... have shown that synaptic levels of the protein clathrin are a determinant factor for synaptic plasticity of neurons. ... The dogma of the clathrin Study investigators have sought to determine the role of clathrin in synaptic plasticity. "This ... presynaptic levels of clathrin decrease reversibly. Ie levels clathrin are a dynamic property of the terminals". "This is not ... Presynaptic Clathrin Levels Are a Limiting Factor for Synaptic Transmission. The Journal of Neuroscience. Doi: 10.1523/JNEUOSCI ...