Biliary alkaline phosphatase measured by mini-column chromatography on DEAE-cellulose: application to detection of...
Using chromatography on diethylaminoethyl (DEAE) cellulose, we measured biliary alkaline phosphatase (BALP; EC 220.127.116.11) ... Biliary alkaline phosphatase measured by mini-column chromatography on DEAE-cellulose: application to detection of ... Biliary alkaline phosphatase measured by mini-column chromatography on DEAE-cellulose: application to detection of ... Biliary alkaline phosphatase measured by mini-column chromatography on DEAE-cellulose: application to detection of ...http://clinchem.aaccjnls.org/content/35/8/1684
Chemical Germplasm Investigations in Soybeans: The Flotsam Hypothesis | SpringerLink
Isolation of four soybean trypsin inhibitors by DEAE-cellulose chromatography. Biochem. Biophys. Res. Commun. 15: 230-235. ... Chromatography of soybean whey proteins on diethylaminoethylcellulose. J. Am. Chem. Soc. 81: 6265-6270.CrossRefGoogle Scholar ...https://link.springer.com/chapter/10.1007/978-1-4684-8309-3_7
Mechanism of Sperm Immobilization by Escherichia coli
2.5.2. DEAE-Cellulose Column Chromatography. The pooled and concentrated fractions after molecular sieving through G-100 were ... Further purification was done by molecular sieving and DEAE-cellulose chromatography. 2.5.1. Molecular Sieving. Purification of ... Figure 2: Elution pattern of sperm immobilization factor from E. coli on DEAE cellulose column. ... These fractions were pooled, concentrated and applied to DEAE cellulose column. The results indicated that most of the SIF ...https://www.hindawi.com/journals/au/2010/240268/
Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond...
A, anion exchange chromatography on DEAE-cellulose open column. B, hydroxyapatite chromatography CHT5 column, FPLC. C, gel ... DEAE-cellulose (5 nmol/min); lane 5, hydroxyapatite (41 nmol/min); lane 6, gel filtration (82 nmol/min); lane 7, Mono Q ... D, anion exchange chromatography on Mono Q 5/50 GL column, FPLC. Fractions were tested for lysoplasmalogenase activity (black ... experiment in which microsomes from seven rat livers were solubilized and purified using these four column chromatography steps ...https://www.ncbi.nlm.nih.gov/pubmed/21515882
CiNii 論文 - What have we learned about sea urchin sperm bindin?
Purification of sea urchin sperm bindin by DEAE-cellulose chromatography VACQUIER V. D. ...http://ci.nii.ac.jp/naid/10008754405
B: Second DEAE-cellulose column chromatography. After loading, the 5-ml column was washed with 30 ml of 25 mmol/l Tris-acetate ... A: First DEAE-cellulose column chromatography. After sample loading, the 15-ml column was washed with 75 ml of binding buffer. ... The supernatant was passed through 0.4-μm filters and then chromatographed on DEAE-cellulose (DE-52, Whatman, 15-ml bed) ... and again chromatographed on DEAE-cellulose (5-ml column). KHK was eluted with a linear gradient of 25-200 mmol/l Tris-acetate ...http://diabetes.diabetesjournals.org/content/52/9/2426
CHROMATOGRAPHY. Hb X was isolated by DEAE-cellulose chromatography. STRUCTURE STUDIES. The tryptic peptides were separated by ... Hb X moves slower than Hb S at alkaline pH (cellulose acetate). ...http://globin.cse.psu.edu/globin/html/huisman/variants/alpha/Cordele.html
CHROMATOGRAPHY. Hb X was isolated by DEAE-cellulose chromatography. STRUCTURE STUDIES. Tryptic digestion; fingerprinting; amino ...http://globin.cse.psu.edu/html/huisman/variants/beta/Riverdale-Bronx.html
CHROMATOGRAPHY. No separations reported except by DEAE-cellulose chromatography. STRUCTURE STUDIES. Tryptic digestion of betaX ...http://globin.cse.psu.edu/html/huisman/variants/beta/Mozhaisk.html
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
by DEAE cellulose chromatography and Sephacryl S-400 size-exclusion chromatography. The structural characterisation, ... The levels of MUC5AC protein and ATP in culture medium were detected by ELISA and high performance liquid chromatography, ...https://www.jove.com/visualize?author=Ting-Feng%20Wu%20
The antibody was dialyzed against phosphate buffer (pH 8.0) and purified by DEAE-cellulose chromatography. ... HbA1c was measured by an HPLC cation-exchange chromatography system (HA-8,121; Kyoto Daiichi, Kyoto, Japan). The intra-assay ... Cai J, Hurst HE: Identification and quantitation of N-(carboxymethyl)valine adduct in hemoglobin by gas chromatography/mass ... CMV-Hb-in circulating erythrocytes by gas chromatography. Our results for the CMV-Hb value of healthy subjects were similar to ...http://care.diabetesjournals.org/content/24/5/891
Ankyrin-Tiam1 Interaction Promotes Rac1 Signaling and Metastatic Breast Tumor Cell Invasion and Migration | JCB
The anti-Tiam1 IgG fraction was prepared by conventional DEAE-cellulose chromatography. Mouse monoclonal anti-hemagglutinin (HA ... B) A Coomassie blue stain of the 116-kD GST-ARD fusion protein purified by affinity column chromatography (lane 1), and the 89- ... obtained from anti-Tiam1 affinity column chromatography) in the absence or the presence of 100-fold excess amount of unlabeled ... and GFP-tagged Tiam1 fragment fusion purified by anti-GFP-conjugated affinity column chromatography (lane 2). (C, a) Binding of ...http://jcb.rupress.org/content/150/1/177
Gene switching in Amoeba proteus caused by endosymbiotic bacteria | Journal of Cell Science
A) Results of Sephadex G-150 chromatography of D and xD amoebae. (B) Results of DEAE-cellulose chromatography of D and xD ... Results of western blot analyses of proteins fractionated by Sephadex G-150 chromatography. Fractions containing SAMS enzyme ...http://jcs.biologists.org/content/117/4/535.figures-only
US Patent # 4,839,293. DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino...
Unincorporated ATP was removed by chromatography on DEAE-cellulose (13). Construction of the genomic library from Streptomyces ...http://patents.com/us-4839293.html
Patent US5643722 - Methods for the detection and isolation of proteins - Google Patents
... phenol extraction followed by chromatography on DEAE-cellulose. Amino-acid specific tRNA, for example tRNAfMet, can be isolated ... reverse-phase chromatography, affinity chromatography and ion exchange chromatography. These techniques are very useful for ... The resulting mixture was applied to a DEAE-cellulose column (1 ml), equilibrated with 50 mM NaOAc, pH 4.5, 10 mM MgCl2, at 4 C ... high-pressure or fast-pressure liquid chromatography, affinity chromatography, ion exchange chromatography, chemical extraction ...http://www.google.ca/patents/US5643722
Schistosoma Mansoni Soluble Egg Antigens | The Journal of Immunology
The eluted antigens were separated from each other by ion exchange chromatography on DEAE cellulose. On polyacrylamide gel ...http://www.jimmunol.org/content/117/5_Part_1/1553
Molecules | Free Full-Text | Pectic Bee Pollen Polysaccharide from Rosa rugosa Alleviates Diet-Induced Hepatic Steatosis and...
RBPP was further fractionated by DEAE-cellulose chromatography into neutral fraction (RBPP-N) and pectic fraction (RBPP-P). ... It was then separated by DEAE-Cellulose column, eluted with water and 0.4 M NaCl, respectively. Two sub-fractions (RBPP-N and ...https://www.mdpi.com/1420-3049/22/5/699/htm
US20100323430A1 - Media For Membrane Ion Exchange Chromatography - Google Patents
Separation of adenovirus by chromatography on DEAE-cellulose. Virology 1961, 13, (2), 264). Membrane chromatography has started ... Chromatography has been used for virus purification, primarily in the format of beads. First reports on chromatography-based ... B01D15/08-Selective adsorption, e.g. chromatography * B01D15/26-Selective adsorption, e.g. chromatography characterised by the ... Chromatography column US4861705A (en) * 1983-01-31. 1989-08-29. Yeda Research And Development Company, Ltd.. Method for ...https://patents.google.com/patent/US20100323430
Ion-exchange chromatography | Article about ion-exchange chromatography by The Free Dictionary
Find out information about ion-exchange chromatography. A chromatographic procedure in which the stationary phase consists of ... ion-exchange resins which may be acidic or basic Explanation of ion-exchange chromatography ... 2010), a SP separated by ion-exchange chromatography (DEAE-cellulose) from S.. Iota-carrageenans from Solieria filiformis ( ... 1]) and subjected to ion-exchange chromatography in a DEAE-cellulose column (Sigma Chemical) equilibrated and percolated with ...http://encyclopedia2.thefreedictionary.com/ion-exchange+chromatography
Hb X elutes between Hb A2 and Hb A on DEAE-cellulose chromatography; the betaX chain separates from the betaA and alpha chains ... CHROMATOGRAPHY. Hb X moves like Hb E in cation exchange HPLC; does not separate from Hb A2; ...http://globin.cse.psu.edu/html/huisman/variants/beta/Sunnybrook.html
Enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44 - Nguyen-Distèche Martine
... chromatography affinity ; chromatography, deae-cellulose ; electrophoresis, paper ; escherichia coli/cytology enzymology ; ...https://orbi.uliege.be/handle/2268/83093
Molecular characterization and classification of Trypanosoma spp. Venezuelan isolates based on microsatellite markers and...
The trypanosomes were expanded in rats and purified by ion exchange chromatography (DEAE-Cellulose) . Parasites were ... Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol. 1970;28(3):521-34.View ...https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-1129-2
Medline ® Abstract for Reference 14 of 'Classification and causes of jaundice or asymptomatic...
... activity for bilirubin and 4-nitrophenol was separated from solubilized rat liver microsomes by DEAE-cellulose chromatography ... enzyme activities were reflected in the enzymatically active fractions separated after DEAE-cellulose chromatography. The ...http://www.uptodate.com/contents/classification-and-causes-of-jaundice-or-asymptomatic-hyperbilirubinemia/abstract/14
Hypersensitivity to Ascaris Antigens | The Journal of Immunology
The crude extract was fractionated by DEAE-cellulose chromatography and the fractions were tested in P-K type reactions. The ...http://www.jimmunol.org/content/101/4/628