Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. | Circulation...
SepPak C-18 purification preceded reverse phase high pressure liquid chromatography. High pressure liquid chromatography ... Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography.. M I Phillips, B ... Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. ... Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. ...http://circres.ahajournals.org/content/56/2/212
Ventricular Hypertrophy Plus Neurohumoral Activation Is Necessary to Alter the Cardiac β-Adrenoceptor System in Experimental...
Plasma noradrenaline content was assessed by high-pressure liquid chromatography (HPLC) by fluorimetric detection.16 ... Samples were centrifuged with 600g for 5 minutes at 4°C, the plasma was removed, quickly frozen in liquid nitrogen, and stored ... Subsequently right and left ventricular tissue was quickly frozen in liquid nitrogen and stored at −80°C until further use. ... To study the course of the development of right ventricular hypertrophy from pressure overload, MCT-treated rats and control ...http://circres.ahajournals.org/content/91/11/1056
Cardiac Overexpression of the Norepinephrine Transporter Uptake-1 Results in Marked Improvement of Heart Failure | Circulation...
The high pressure liquid chromatography-based NE detection system used in this investigation is highly specific for NE and ... and measured by high performance liquid chromatography with electrochemical detection. ... Left ventricular pressure was significantly increased in Ad-uptake-1-GFP-infected failing rabbits (Ad-GFP 60±4 mm Hg versus Ad- ... Kawai et al16 showed higher LVEDP in rabbits with pacing-induced heart failure than our study, but all other hemodynamic ...http://circres.ahajournals.org/content/97/9/928
Functional Coupling Between Glycolysis and Sarcoplasmic Reticulum Ca2+ Transport | Circulation Research
One hundred microliters of each sample solution was injected on to a high-pressure liquid chromatography (HPLC) column (C18 ... detected by high-performance liquid chromatography of supernatant (bottom). A, ATP and ADP standards, which elute at 4.9 and ... This was possible because hexokinase has a Km for ATP of ≈200 μmol/L,16 whereas the Km of the SR Ca2+-ATPase is higher (0.4 to ... The diffusion gradient for bulk ATP may therefore be higher in vivo, and intracellular ATP compartmentation may be of ...http://circres.ahajournals.org/content/77/1/88
Role of right and left atria in natriuresis and atrial natriuretic factor release during blood volume changes in the conscious...
High-performance liquid chromatography of plasma from the control groups indicated that most of the released ANF during blood ... No significant changes in blood pressure or hematocrit were noted. Plasma vasopressin, measured at the end of volume expansion ... volume expansion corresponded to a high molecular weight peptide. Additional rats, processed as above, were subjected to 10% ...http://circres.ahajournals.org/content/61/1/99
Induction of Vascular Insulin Resistance and Endothelin-1 Expression and Acceleration of Atherosclerosis by the Overexpression...
The lipoprotein profile of cholesterol also was characterized by fast protein liquid chromatography to assess very-low-density ... Blood pressure, as measured by tail vein plethysmography, was similar for systolic blood pressure (112±6 vs 121±10 mm Hg) and ... The necrosis area was 1.99±0.55-times higher in Tg(Prkcb)apoE−/− mice compared with apoE−/− mice (11.0±3.1% vs 5.5±2.5%; P,0.05 ... Mechanism of high glucose induced angiotensin II production in rat vascular smooth muscle cells. Circ Res. 2007;101:455-464. ...http://circres.ahajournals.org/content/113/4/418
Loss of Angiotensin-Converting Enzyme-2 Exacerbates Diabetic Cardiovascular Complications and Leads to Systolic and Vascular...
... were performed by high-performance liquid chromatography as previously described.16 Left ventricular (LV) myocardial ACE2 ... Systolic blood pressure of each mouse was measured by the tail-cuff method with an IITC blood pressure monitoring system (IITC ... Hypoxia and high glucose upregulate at1 receptor expression and potentiate ANG II-induced proliferation in VSM cells. Am J ... In vivo measurement of flow-mediated vasodilation in living rats using high-resolution ultrasound. Am J Physiol Heart Circ ...http://circres.ahajournals.org/content/110/10/1322
Endothelial Cells Provide Feedback Control for Vascular Remodeling Through a Mechanosensitive Autocrine TGF-β Signaling Pathway...
High pressure was set at 200 mm Hg and normal pressure at 100 mm Hg, as measured using a pressure sensor (Harvard Apparatus). ... White bars=media depleted of perlecan by affinity chromatography. *P,0.05 vs control samples; **P,0.05 vs all other samples. ... Values shown are for liquid scintillation readings of 3H-glucosamine incorporation only. A, Samples treated with 5% strain ( ... mean pressure of 100 mm Hg; Figure 6B and 6C). Inhibitors to TGF-β and MEK1/2 (U0126) blocked high pressure-induced expression ...http://circres.ahajournals.org/content/103/3/289
Endothelin-1 and Angiotensin II Receptors in Cells From Rat Hypertrophied Heart | Circulation Research
... by the lactoperoxidase method and purified by high-performance liquid chromatography.33 For ET-1 receptors, the competitive ... Chronic volume or pressure overload induces a series of cellular and molecular events leading to an adaptive cardiac ... However, [Ca2+]i responses induced by Ang II at concentrations ,10−8 mol/L were detectable and were significantly higher in ... Saturation experiments with cultured ventricular fibroblasts showed that 125I-[Sar1,Ile8]Ang II bound with high affinity to a ...http://circres.ahajournals.org/content/78/2/302
Identification of Epoxyeicosatrienoic Acids as Endothelium-Derived Hyperpolarizing Factors | Circulation Research
gas chromatograph (chromatography). HPLC. =. high-performance liquid chromatography. MS. =. mass spectrometer (spectrometry). ... Perfusion pressure was maintained at 60 mm Hg by an outflow restrictor and measured continuously by a pressure transducer. The ... The relaxations were also blocked by TEA, an inhibitor of Ca2+-activated K+ channels, and by high [K+]o (20 mmol/L) (Fig 1A⇓). ... A high-resistance seal (1 to 10 GΩ) was formed between the pipette and the cell membrane by applying a slight suction. After ...http://circres.ahajournals.org/content/78/3/415
Inhibition of Cytochrome P450ω-Hydroxylase | Circulation Research
Microsome Preparation and High-Performance Liquid Chromatography Analysis of 20-HETE Production. Heart tissue was homogenized ... There were no significant differences in heart rate, mean arterial blood pressure, and pressure-rate product at baseline ... and then the samples were analyzed by high-performance liquid chromatography and fractions were counted for radioactivity. A ... Atelectasis was prevented by maintaining an end-expiratory pressure of 5 to 7 cm-H2O with a trap. Arterial blood pH, pCO2, and ...http://circres.ahajournals.org/content/95/8/e65
Protective Role of Bradykinin in Cardiac Anaphylaxis | Circulation Research
Histamine was assayed by reverse-phase high-performance liquid chromatography (HPLC) using an isocratic system.16 17 The HPLC ... After midline thoracotomy, the heart was rapidly excised and perfused retrogradely at a constant pressure of 45 cm H2O in a ... Determination of histamines in wines and musts by reversed-phase high performance chromatography. J Chromatogr. 1978;166:310- ... The expression of high molecular weight kininogen on human umbilical vein endothelial cells. J Biol Chem. 1988;31:16327-16333. ...http://circres.ahajournals.org/content/76/3/434
TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial InfarctionNovelty and Significance |...
... as confirmed by mass spectrometry and analytic reversed-phase high-performance liquid chromatography. These peptides were free ... and ventilated with an Inspira Advanced Safety Single Animal Pressure/Volume Controlled Ventilator (Harvard Apparatus). The ... We found that increased concentrations of sTREM-1 at the time of admission in patients with acute MI was related to higher risk ... Moreover, although Trem-1 inhibition decreases Ccl-2 production and thus prevents from the accumulation of Ly-6Chigh cells, it ...http://circres.ahajournals.org/content/116/11/1772
Preferential Oxidation of Triacylglyceride-Derived Fatty Acids in Heart Is Augmented by the Nuclear Receptor PPARα |...
14 The fractional 13C enrichment of TAG was assessed by liquid chromatography/mass spectrometry. LCFA content in TAG, of carbon ... Baseline rate pressure products (RPPs) were similar between hearts of transgenic (MHC-PPARα) and NTG mice: MHC-PPARα: 35 000± ... Palmitate turnover within the TAG pool of MHC-PPARα hearts was 4-fold higher than that of NTG hearts. Unlike NTG, MHC-PPARα ... Left ventricular developed pressure and heart rate were continuously recorded from a water-filled balloon in the left ventricle ...http://circres.ahajournals.org/content/107/2/233.long
Stoichiometric Relationships Between Endothelial Tetrahydrobiopterin, Endothelial NO Synthase (eNOS) Activity, and eNOS...
... and neopterin were measured in aortic homogenates by high-performance liquid chromatography (HPLC) analysis after iodine ... regulates vasomotor tone and blood pressure,1,2 and has multiple antiatherogenic roles by inhibiting vascular smooth muscle ... After incubation, aortas were snap-frozen in a column of Krebs-HEPES buffer in liquid nitrogen, and EPR spectra were obtained ... For each vessel, mean fluorescence was calculated from 4 separate high-power fields taken in each quadrant of the vessel to ...http://circres.ahajournals.org/content/97/9/864
Gut Microbiota-Dependent Trimethylamine N-Oxide (TMAO) Pathway Contributes to Both Development of Renal Insufficiency and...
Trimethylamine-N-oxide (TMAO) levels were determined by stable isotope dilution high-performance liquid chromatography with ... Adjustments were made for individual traditional risk factors, including age, sex, systolic blood pressure, low-density ... Measurement of trimethylamine-N-oxide by stable isotope dilution liquid chromatography tandem mass spectrometry. Anal Biochem. ... In subjects without CKD, higher levels of TMAO portend poorer survival, both among those with high or low levels of cystatin C ...http://circres.ahajournals.org/content/116/3/448
Production of 20-HETE and Its Role in Autoregulation of Cerebral Blood Flow | Circulation Research
... pressure from 20 to 140 mm Hg increased 20-HETE concentration by 6-fold in cerebral arteries as measured by gas chromatography/ ... The high degree of homology between the P-450 4A isoforms suggests that the antibody and cRNA probes used in the in situ ... In a separate study, the arteries were pressurized at 20 or 140 mm Hg for 30 minutes, removed, and frozen in liquid N2, and 20- ... Effect of Transmural Pressure on 20-HETE Concentration. Middle cerebral arteries (150 to 200 μm ID) were placed in a pressure ...http://circres.ahajournals.org/content/87/1/60
Heparanase Alters Arterial Structure, Mechanics, and Repair Following Endovascular Stenting in Mice | Circulation Research
... the arterial blood pressure in the mice was measured using a tail cuff blood pressure monitor (Holliston, Mass). The average ... Heparanase upregulation in high glucose-treated endothelial cells is prevented by insulin and heparin. Exp Biol Med (Maywood). ... One-milliliter fractions were collected, and aliquots of these samples were counted using liquid scintillation. Aliquots were ... Cell lysates and conditioned media were then analyzed for glycosaminoglycan content using ion exchange chromatography. ...http://circres.ahajournals.org/content/104/3/380
Quantification of Myotrophin From Spontaneously Hypertensive and Normal Rat Hearts | Circulation Research
Solvents used were of high-performance liquid chromatography (HPLC) grade and were purchased from Baxter Healthcare Corp. DVF12 ... A dissociation between blood pressure and development of hypertrophy has been demonstrated by many investigators.22 23 24 25 26 ... As shown here, in neonatal rats, the myotrophin level was found to be high; as the animals grew older, the myotrophin level ... of a trifunctional amino acid as the core matrix and 2n peptide antigens to form a macromolecule with a high density of peptide ...http://circres.ahajournals.org/content/76/6/1020
A Subpopulation of Cardiomyocytes Expressing α-Skeletal Actin Is Identified by a Specific Polyclonal Antibody | Circulation...
Switches in cardiac muscle gene expression as a result of pressure and volume overload. Am J Physiol. 1992;262:R364-R369. ... Thus a high percentage of myocytes expresses both proteins, although this is not uniformly the case. Furthermore, during the ... Using sequential affinity chromatography, we recovered from serum antibodies reacting exclusively with α-skeletal actin when ... Tissue samples were embedded in OCT 4583 (Miles Scientific) and frozen in precooled liquid isopentane. Three-μm cryostat ...http://circres.ahajournals.org/content/85/10/e51