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*  中国科学院大连化学物理研究所机构知识库(DICP OpenIR): Tumor anti-angiogenic gene therapy with microencapsulated
Twenty days after tumor cell injection, 80% of animals treated with microencapsulated CHO-endo cells were alive compared to ... Tumor anti-angiogenic gene therapy with microencapsulated recombinant CHO cells. 作者: Zhang, Ying; Wang, Wei; Zhou, Jing; Yu, ... In this paper, APA microcapsules with high strength kept intact over 35 days and recombinant CHO cells kept the rapid ... The study of tumor treatment showed that the implantation of microencapsulated recombinant CHO cells decreased the ...
http://cas-ir.dicp.ac.cn/handle/321008/98543
*  WikiGenes - CHO Cells
Importantly, in cell-cell adhesion assays between CD2+ Jurkat T cells and CD48- or CD59-transfected CHO cells, there was no ... Gene context of CHO Cells. *Fork slowing is reduced or absent in irs1SF CHO cells and XRCC3(-/-) chicken DT40 cells, indicating ... cells [15].. *Maximal sensitization of the CHO cells toward ricin and Pseudomonas toxin requires preculture of CHO cells in the ... The CHO cell lines, IFN-gamma-treated human peripheral-blood monocytes, and IFN-gamma-treated cells of the human monocytic cell ...
https://www.wikigenes.org/e/mesh/e/10946.html
*  Sack: Freezing CHO cells - OpenWetWare
Gently resuspend cell pellet in 2 ml freezing medium*.. * Transfer 0.5 ml to 4 freezing vials labeled with name of cell line, ... Digest cells with 3 ml Trypsin/EDTA, wait for cells to detach. ... Freezing CHO Cells. Christina McGee 4-5-2012. * Grow 10 cm dish ... Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO ( ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Sack:_Freezing_CHO_cells&oldid=667947" ...
https://openwetware.org/wiki/Sack:_Freezing_CHO_cells
*  flp-in cho Cells | Thermo Fisher Scientific - CA
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... Developed by IVGN from CHO-K1 Chinese Hamster ovary (Kao and Puck, 1968) ATCC# CCL-61 also referred to as CRL-9618.. ... To give you confidence in the health of your cells every step of the way, ...
https://www.thermofisher.com/ca/en/home/technical-resources/cell-lines/f/cell-lines-detail-538.html
*  CHO Cells
Stem Cell Products including NutriStem hPSC and MSC media, CryoStem Freezing Media, and other serum-free, xeno-free media ... Over 30 years of experience in developing and manufacturing a diverse cell culture media, including Sera, FBS, ... BIOGRO-CHO SFM Supplement (100X). For use with BIOCHO-1 SFM BASE, 5 mL. Learn More ... BIO-CHO-1 SFM Base Serum-free medium, 500 mL Learn More ... Stem Cell Research *Stem Cell Culture. *Stem Cell ...
http://www.bioind.com/products/cell-culture/specialty-media/cho-cells/
*  Serum-free large-scale transient transfection of CHO cells
Keywords: Animals ; Bioreactors ; CHO Cells/*physiology ; Cell Culture Techniques/methods ; Cell Proliferation ; Cell Survival ... CHO) cells in the absence of serum. CHO-DG44 cells were cultivated and transfected in a chemically defined medium using linear ... Serum-free large-scale transient transfection of CHO cells. Derouazi, M.; Girard, P.; Van Tilborgh, F.; Iglesias, K.; Muller, N ... The highest levels of r-protein expression were observed when cultures at a density of 2.0 x 10(6) cells/ml were transfected ...
https://infoscience.epfl.ch/record/105019
*  Adhering CHO cell culture - Tissue and Cell Culture - BioForum
... cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day- ... posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) ... Are these floating cells dying or just not attaching, because CHO cells can also grow as a suspension culture, though i don't ... Have you done a viability assay to ensure that the floating cells are, in fact, dead?. CHO cells are damned hardy and will ...
http://www.protocol-online.org/forums/topic/15050-adhering-cho-cell-culture/
*  Interaction between 2N-methyl-9-hydroxyellipticinium and ionizing radiation in CHO cells.
The main effect is observed in the case of exponentially growing cells which are also more sensitive to the drug alone than ... increases the killing of gamma-irradiated CHO cells. ... CHO Cells / radiation effects*. Cell Survival / radiation ... Previous Document: Targets for radiation-induced cell death: target replication during the cell cycle evaluated in cell.... ... increases the killing of gamma-irradiated CHO cells. The main effect is observed in the case of exponentially growing cells ...
http://www.biomedsearch.com/nih/Interaction-between-2N-methyl-9/8103546.html
*  serumm free culture of adherent cho cells - Tissue and Cell Culture - BioForum
i donot prefer to make suspension culture of the cho cells as that may change some properties of the cho cells. any suggestions ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ... posted in Tissue and Cell Culture: Hi all, ... cho cells as that may change some properties of the cho cells. ... i usually culture my cho cells in f12 medium with 10% fcs. but now i would like to culture the cho cells in serum free media ...
http://www.protocol-online.org/forums/topic/26144-serumm-free-culture-of-adherent-cho-cells/
*  CHO cell | definition of CHO cell by Medical dictionary
CHO cell explanation free. What is CHO cell? Meaning of CHO cell medical term. What does CHO cell mean? ... Looking for online definition of CHO cell in the Medical Dictionary? ... CHO cell , definition of CHO cell by Medical dictionary https://medical-dictionary.thefreedictionary.com/CHO+cell ... Chinese Hamster Ovary Cell. (redirected from CHO cell) A line of fibroblasts isolated in 1958, which is used in research, grows ...
https://medical-dictionary.thefreedictionary.com/CHO+cell
*  Selection of chemically defined media for CHO cell fed-batch culture processes
... Pan, Xiao; Streefland, Mathieu; Dalm, Ciska; ... Pan, X., Streefland, M., Dalm, C., Wijffels, R.H. & Martens, D.E. (2016). Selection of chemically defined media for CHO cell ...
https://brage.bibsys.no/xmlui/handle/11250/2433531
*  Rapid development of stable transgene CHO cell lines by CRISPR/Cas9-mediated site-specific integration into C12orf35 |...
... cells are the most widely used mammalian hosts for recombinant protein production. However, by conventional random integration ... Hiller GW, Ovalle AM, Gagnon MP, Curran ML, Wang WG (2017) Cell-controlled hybrid perfusion fed-batch CHO cell process provides ... CHO Site-specific integration CRISPR/Cas9 Cell line development C12orf35 Electronic supplementary material. The online version ... Li S, Gao X, Peng R, Zhang S, Fu W, Zou F (2016) FISH-based analysis of clonally derived CHO cell populations reveals high ...
https://link.springer.com/article/10.1007%2Fs00253-018-9021-6
*  SILu™MAb Mouse Stable-Isotope Labeled Monoclonal Antibody from mouse, recombinant, expressed in CHO cells | Sigma-Aldrich
... expressed in CHO cells; find Sigma-Aldrich-MSQC10 MSDS, related peer-reviewed papers, technical documents, similar products & ... Expressed in CHO cells, SILu™MAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal ... Expressed in CHO cells, SILuMAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal ...
https://www.sigmaaldrich.com/catalog/product/sigma/msqc10?lang=en®ion=US
*  Nickel and cobalt affect galactosylation of recombinant IgG expressed in CHO cells | Springer for Research & Development
Ha TK, Hansen AH, Kol S, Kildegaard HF, Lee GMC (2017) Baicalein reduces oxidative stress in CHO cell cultures and improves ... Yuk IH et al (2015) Effects of copper on CHO cells: cellular requirements and product quality considerations. Biotechnol Prog ... Grainger RK, James DC (2013) CHO cell line specific prediction and control of recombinant monoclonal antibody N-glycosylation. ... Prabhu A, Gadre R, Gadgil M (2018) Zinc supplementation decreases galactosylation of recombinant IgG in CHO cells. Appl ...
https://rd.springer.com/article/10.1007%2Fs10534-018-0152-0
*  Mutagenesis and directed evolution in CHO cells to improve bio-production characteristics - DORAS
Sterritt, Neil (2013) Mutagenesis and directed evolution in CHO cells to improve bio-production characteristics. PhD thesis, ... Infecting cells with this virus at an MOI of over 2 x 105 particles per cell, we have demonstrated that this unique genomic ... Mutagenesis and directed evolution in CHO cells to improve bio-production characteristics ... Biological Sciences , Cell biology. Biological Sciences , Molecular biology. DCU Faculties and Centres:. DCU Faculties and ...
http://doras.dcu.ie/18612/
*  Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO Cells
They also have the potential to be the best signal peptides for the production of new antibodies in CHO cells. ... develop a platform technology to optimize the signal peptides for high level production of therapeutic antibodies in CHO cells ... CHO cells Is the Subject Area "CHO cells" applicable to this article? Yes. No. ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116878
*  Frontiers | Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing Antibodies |...
Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields ... Here, we report the development of a CHO-M cell line that expressed BG505 NFL trimers at a high level of homogeneity and yields ... SUREtechnology™ Cell Line Development Platform. The Selexis CHO-M host cell line is derived from CHO-K1 CCL-61 cells from the ... general cell metabolism (38). We applied the CHO-Mplus libraries approach to BG505 NFL expressing CHO-M cells to support proper ...
https://www.frontiersin.org/articles/10.3389/fimmu.2018.01116/full
*  Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells | Sigma-Aldrich
reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. ... The host cell line (CHOZN® GS) had significantly higher endogenous Mgat1 expression than the IgG expressing cell line. Mild ... reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells. The hypothesis is that Mgat1 ... Overexpressing Mgat1 in wildtype CHO cells did not lead to increased sialylation ...
https://www.sigmaaldrich.com/technical-documents/articles/biology/mgat1-disrupted-chinese-hamster-ovary-cells.html
*  microRNAs as potential tools for 'miR'aculous CHO cell phenotypes in Bioprocessing systems - DORAS
... of our research group to manipulate these miRNAs in CHO cells with a view to positively impact bioprocess-relevant CHO cell ... Chinese hamster ovary; CHO; miRNA; CRISPR-Cas9. Subjects:. Biological Sciences , Biotechnology. Biological Sciences , Cell ... Chinese Hamster Ovary (CHO) cells are the biopharmaceutical industry's "mini biofactories" for the production of complex, post- ... In the second approach, we targeted a previously verified miRNA for improved CHO cell growth and productivity i.e. miR-7, using ...
http://doras.dcu.ie/21328/
*  Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor ...
CHO,Cells,Expressing,the,Human,5-HT1B,Receptor,biological,advanced biology technology,biology laboratory technology,biology ... CHO cells expressing the h5-HT1B receptor were diluted to a desired membrane protein concentration in 25 mM HEPES buffer ... Prior to [35S]-GTPγS binding assay, the CHO cells were prepared as described by Thomas, et al ., 1995, and frozen in aliquots ... Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor ...
http://www.bio-medicine.org/biology-technology/Use-of-FlashPlate-Technology-for-In-Vitro-Measurement-of--5B35S-5D-GTP-26-23947-3BS-Binding-in-CHO-Cells-Expressing-the-Human-5-HT1B-Receptor-1378-1/