Chronotropism and blood flow patterns following teratogenic doses of catecholamines in 5-day-old chick embryos.
In vivo heart rates of 5-day-old chick embryos were recorded from electrodes placed in close proximity to the heart. L-epinephrine (4X10(-10) mole), 1-norepinephrine (1X10(-9)mole) and 1-isoproterenol (1.6X10(-10)mole) in 5 microliter of isotonic salhttp://www.biomedsearch.com/nih/Chronotropism-blood-flow-patterns-following/22939.html
Amino acid uptake in the developing chick embryo heart. The effect of insulin on glycine and leucine accumulation. - Semantic...
1. The accumulation of [1-(14)C]glycine and the uptake, accumulation, incorporation (into protein, lipid, glycogen) and oxidation of l-[1-(14)C]leucine in 5-day-old chick embryo hearts were investigated in vitro, and the effects of insulin, puromycin and 4-methyl-2-oxopentanoic acid on these processes were studied. 2. With glycine, the ratio of concentration of the labelled amino acid in the cell water to that in medium markedly exceeded unity. Insulin significantly increased this ratio. Puromycin did not prevent the insulin effect. 3. With leucine, the concentration ratio of the labelled amino acid between intracellular and extracellular water approached unity in the absence of puromycin and was doubled by its presence. In neither case did insulin substantially alter this ratio. The addition of 4-methyl-2-oxopentanoic acid had no effect in the absence of insulin, but produced a significant increase of the concentration ratio in the presence of the hormone. 4. Leucine uptake ...https://www.semanticscholar.org/paper/Amino-acid-uptake-in-the-developing-chick-embryo-h-Guidotti-Gaja/ed50b4f9e566efb873db4275c54f17328015bd14
Control of cytosolic calcium activity during low sodium exposure in cultured chick heart cells. | Circulation Research
We investigated the roles of sodium-calcium exchange, sarcoplasmic reticulum, and mitochondria in Cai homeostasis in cultured chick ventricular cells. Specifically, the influence of low sodium medium on contractile state, calcium fluxes, and cytosolic free [Ca] [( Ca]i) was examined. [Ca]i was measured using fura-2. Mean [Ca]i in control medium was 126 +/- 14 nM. Exposure of cells to sodium-free or sodium- and calcium-free medium (choline-substituted) resulted in contracture development, which returned toward the baseline level over 2-3 minutes. The Nao-free contracture was associated with a tenfold increase in [Ca]i (1,280 +/- 110 nM) followed by a gradual decrease to a level fourfold above control [Ca]i (460 +/- 58 nM). Nao- and Cao-free contracture was associated with a fivefold increase in [Ca]i (540 +/- 52 nM) followed by a rapid decrease to below 80 nM. Sodium-free medium failed to produce an increase in [Ca]i or contracture in cells preexposed to calcium-free medium, although ...http://circres.ahajournals.org/content/61/1/29
Acetylcholine, acetyl-β-methylcholine and carbamylcholine inhibited sinoatrial pacemaker activity in atria isolated from chick embryos on the 6th, 12th and 18th incubation days. Cardioinhibition persisted throughout the 5-minute exposure to cholinergic agonists in sinoatrial preparations from the 12th and l8th incubation days whereas automaticity recovered completely within 1 to 2 minutes in preparations from the 6th incubation day. Neither hydrolysis by acetylcholinesterase nor release of catecholamines from cardiac stores accounted adequately for the brevity of pacemaker inhibition by choline esters on the 6th incubation day. Evidence suggested that desensitization, most prominently observed with carbamylcholine, restricted the duration of cholinergic inhibition of the pacemaker on the 6th incubation day. Although automaticity recovered completely within 2 minutes after initial application of acetylcholine and carbamylcholine, pacemaker cells were not inhibited by a second ...http://jpet.aspetjournals.org/content/191/1/109
Head process synonyms, head process antonyms - FreeThesaurus.com
Synonyms for head process in Free Thesaurus. Antonyms for head process. 352 synonyms for head: skull, crown, pate, bean, nut, loaf, cranium, conk, noggin, noddle, mind, reasoning, understanding, thought, sense, brain, brains.... What are synonyms for head process?http://www.freethesaurus.com/head+process
Abstract: Ph. M. Hemken, R. M. Bellin, S. W. Sernett, B. Becker, T. W. Huiatt, R. M. Robson (1997)
Paranemin was initially found to copurify with the intermediate filament (IF) proteins vimentin and desmin from embryonic chick skeletal muscle and was described as an IF-associated protein (IFAP). We have purified paranemin from embryonic chick skeletal muscle, prepared antibodies, and demonstrated that they label at the Z-lines of both adult avian and porcine cardiac and skeletal muscle myofibrils. We determined the cDNA sequence of paranemin by immunoscreening a λgt22A cDNA library from embryonic chick skeletal muscle. Northern blot analysis revealed a single transcript of 5.3 kilobases, which is much smaller than predicted from the size of paranemin (280 kDa) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The derived amino acid sequence of paranemin (1,606 residues; 178,161 kDa) contains the conserved IF rod domain (308 amino acids), which has highest homology to the rod domains of nestin ...http://www.agnld.uni-potsdam.de/~marwan/literature.php?label=hemken1997
Developmental changes in regulation of embryonic chick heart g...
Developmental changes in regulation of embryonic chick heart gap junctions.: Embryonic chick myocyte pairs were isolated from ventricular tissue of 4-day, 14-dahttps://www.mysciencework.com/publication/show/developmental-changes-regulation-embryonic-chick-heart-gap-junctions-e7a56287
EMBRYONIC CHICK INTESTINE IN ORGAN CULTURE | JCB
Duodena from 20-day-old chick embryos can be maintained in large scale organ culture on specially designed stainless-steel grids in contact with serum-free medium for 48 h with excellent preservation of mucosal structure at both the light and electron microscope levels. Although mitotic rate was subnormal, several other factors attest to the essential viability of the cultured intestine: L-leucine incorporation into protein, as well as the synthesis of a specific vitamin D3-induced calcium-binding protein (CaBP), increased over a 48-h culture period, and the electropotential gradient across the intestine was maintained throughout the culture period as was a concentration gradient for calcium. The tissue responded to vitamin D3 in the medium by synthesizing the calcium-binding protein within 6 h and by exhibiting enhanced 45Ca uptake within 12-24 h. Concentrations of vitamin D3, or its 25-hydroxylated derivative, higher than necessary for CaBP induction, also increased the ...http://jcb.rupress.org/content/58/1/64
Request for Info
Hi, I'm looking for a commercial source of primary chicken embryo fibroblast (CEF) culture. Any leads? Thanks, Vladimir Yamshchikov ...http://www.bio.net/bionet/mm/virology/1995-September/004380.html
Examining Cell Movements in the Neurulating Chick Embryo
The avian embryo is a popular animal model because it is widely available (Antin et al., 2004), it is easily manipulated, and it can provide important insights into normal and abnormal embryo development (Kulesa, 2004). While in vivo and in vitro cultures of chick embryos are common, in ovo cultures are rarer, and none have been designed where the egg did not have to be resealed afterwards. The present study aimed to develop a set-up in which the egg would be windowed without resealing the egg so that the embryo would remain accessible for experimental manipulation. As well, this study aimed to track cell movement during neurulation by microinjecting points of dye along the embryo. Two prototypes were developed based on the concept that temperature and moisture controlled air passing over the windowed egg would serve as a blanket. When these prototypes were unable to keep the embryo alive, a ...https://uwspace.uwaterloo.ca/handle/10012/3532
The development in vitro of the blood of the early chick embryo | Proceedings of the Royal Society of London B: Biological...
In a series of tissue cultures of fragments taken from different parts of young chick embryos, at various stages, it was found that differentiation frequently occurred, the characteristic cells of highly specialised tissues appearing in cultures in which they could not possibly have been present at explantation. The most striking differentiations were the development of red blood corpuscles, capillary vessels, nerve cells with axons and of rhythmically contracting cardiac muscle. This paper describes a simple method by which may be obtained regularly and quickly the development of very large numbers of red cells, and an account is given of the histology of the cultures. It is intended to be introductory to a physiological study, now in progress, of the conditions of hæmatopoiesis Literature. Erythropoiesis in vitro has been reported by several authors, but it has not been thoroughly investigated and, with the exceptions of the works of Slonimski (1930, a, 1931) and Shipley ...http://rspb.royalsocietypublishing.org/content/111/773/497
Sequence of Atrial Depolarization at Different Stages of Development of the Chick Embryo | Circulation Research
Simultaneous left and right atrial surface electrograms were obtained from the right and left atria of 30 chick embryos. These were divided into 3 equal groups that were studied at 15 to 16, 10 to 11, and 5 to 7 days of incubation. The embryos were removed from the shell with their circulation intact and temperature maintained constant. In an early stage of development (5 to 6 days), the left atrium and its appendage were larger than the right, and left preceded right atrial depolarization. At and after 7 days of incubation, the right atrium and its appendage were larger than the left, and right preceded left atrial depolarization. The intervals for interatrial conduction and for atrio-ventricular conduction were about the same at all stages of development. Since the length and width of the heart at 15 to 16 days of incubation were about 3 times those of a heart at 5 to 7 days of incubation, it is proposed that the higher the stage of development of the ...http://circres.ahajournals.org/content/20/2/204
Divergent kinetics of 201Tl and 99mTc-SESTAMIBI in cultured chick ventricular myocytes during ATP depletion. | Circulation
METHODS AND RESULTS To better understand the mechanistic responses of these tracers to myocellular injury, cultured chick embryo cardiac myocytes were metabolically inhibited in iodoacetate (1 mM) and rotenone (10 microM) for up to 2 hours, and initial uptake rates of each agent were determined at successive intervals along with correlative cellular contents of ATP, sodium, and potassium and lactate dehydrogenase release. ATP content fell from 30.5 +/- 1.4 to 2.7 +/- 0.9 nmol.(mg protein)-1 within 2 minutes, whereas sodium and potassium contents ran down their thermodynamic gradients more slowly (t 1/2 approximately 60 minutes). Modestly severe cell injury was produced at 2 hours as estimated by lactate dehydrogenase release (18% of total). Initial uptake rates of 201Tl declined from 6.9 +/- 0.8 to 4.0 +/- 0.4 fmol.(mg protein)-1.(nMo)-1.(min)-1 by 20 minutes and remained depressed and ouabain (100 microM)-insensitive at 30 +/- 13% of control. Conversely, initial uptake ...http://circ.ahajournals.org/content/85/4/1531
A method for the determination of red cell mass, which employs the use of radioisotopically-labeled erythrocytes, in the embryonated hen egg is described and results of total blood volume, plasma volume, red cell mass and red cell concentration determinations during the period from 9 to 18 days of incubation presented. Considerations of the values obtained in terms of absolute quantities and relative to embryonic mass are discussed. It is suggested that expansion of the red cell mass and somatic growth are portions of a common process and may be subject to a complex of similar regulatory factors in the normal chick embryo.. ...http://ajplegacy.physiology.org/content/197/2/403
Most recent papers with the keyword Haitian | Read by QxMD
BACKGROUND: Chicken embryos are widely used as a model for studies of obesity; however, no detailed information is available about the dynamic changes of proteins during the regulation of adipose biology and metabolism. Thus, the present study used an isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic approach to identify the changes in protein abundance at different stages of chicken embryonic development. RESULTS: In this study, the abundances of 293 hepatic proteins in 19-day old of chicken embryos compared with 14-day old and 160 hepatic proteins at hatching compared with 19-day old embryos were significantly changed ...https://www.readbyqxmd.com/keyword/7978
Exploring the Caffeine-Induced Teratogenicity on Neurodevelopment Using Early Chick Embryo
Caffeine consumption is worldwide. It has been part of our diet for many centuries; indwelled in our foods, drinks, and medicines. It is often perceived as ahttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034278
Memorial Day Chicken Papaya No Flour Recipes | Foodily
See all results for Memorial Day Chicken Papaya No Flour Recipes. Find more recipes at Foodily.com - the largest recipe search site - and see the ones your friends like.http://www.foodily.com/s/memorial-day-chicken-papaya-no-flour
Developmentally regulated neural protein EAP-300 is expressed by myocardium and cardiac neural crest during chick embryogenesis...
Wiley Online Library will be unavailable on Saturday 7th Oct from 03.00 EDT / 08:00 BST / 12:30 IST / 15.00 SGT to 08.00 EDT / 13.00 BST / 17:30 IST / 20.00 SGT and Sunday 8th Oct from 03.00 EDT / 08:00 BST / 12:30 IST / 15.00 SGT to 06.00 EDT / 11.00 BST / 15:30 IST / 18.00 SGT for essential maintenance. Apologies for the inconvenience caused ...http://onlinelibrary.wiley.com/doi/10.1002/aja.1002030106/references
Characterization of a chicken retinoid X receptor-γ gene promoter and identification of sequences that direct expression in...
Development of the cellular complexity of the vertebrate neural retina relies on an intricate interplay between extracellular signals and intracellular factors. In particular, transcription factors play a key role in determining the competence of cells to respond to extracellular signals. We have previously shown that, in the developing chick neural retina, expression of the retinoid X receptor-γ (RXR-γ2) nuclear receptor gene is restricted to photoreceptors. To characterize the mechanisms that regulate expression of this gene in the neural retina, we isolated a chicken RXR-γ genomic clone containing the RXR-γ2 promoter and mapped the transcription initiation site by means of ribonuclease protection. We analysed promoter activity by transient transfection of luciferase reporter gene constructs into cultured cells isolated from embryonic-chick neural retina or facial mesenchyme, which does not normally express detectable RXR-γ2 transcripts. ...http://www.biochemj.org/content/347/2/485
New Chicks on the Block: Animal Behavior Students Raise Baby Chickens - The Phillipian
Since Ms. Cormier was willing to house the chicks on her farm after the class finished with them, I decided that bringing back, and even expanding, this work with live chicks seemed like a really good fit for my vision for the course," said Bailey.. Unfortunately, there have been incidents of chicks passing away. After a power outage in Gelb while the eggs were still incubating, the humidity dropped very low in the incubator. The chicks were already partially hatched during this time, causing the exposed membrane to dry up. According to students interviewed by The Phillipian, two chicks did not hatch as a result of the outage.. The first 36 hours after hatching are a critical time for the chicks - after this period, the birds switch from innate imprinting to associative learning and will not be able to imprint on their "mother." According to students, another factor that facilitates the imprinting process is ...http://phillipian.net/2017/09/22/new-chicks-block-animal-behavior-students-raise-baby-chickens/
RNase Protection Assay-HELP!
Hello, everybody! Does anyone have any experience with RNase Protection Assay? I have been doing it for a few months now, using a non-radioactive method for detection (biotin) and I have problems with transferring from the PAGE to a nylon membrane (low efficiency of transfer). My other problem is that after I stain the urea/PAGE with EtBr I see the same band pattern with my control probe (beta-actin) and with my probe (in this case for titin). It's absolutely the same picture-with actin, with titin and with both probes. What I am looking for is differential expression of titin at different stages of primary chick embryo muscle culture and if there is no way of comparing titin's expression with that of actin (housekeeping gene), I am in a bad shape. I would greatly appreciate any help! Thanks a lot in advance! Vesselina Ivanova Graduate student in the Department of Biochemistry and Biophysics Iowa State University -- Vesselina Ivanova vivanova at iastate.edu ...http://www.bio.net/bionet/mm/methods/1997-May/057432.html
Manipulating Gene Expression in the Chick Embryo
Voiculescu O, Stern CD. Manipulating Gene Expression in the Chick Embryo. Methods Mol Biol. 2017;1565:105-114. doi: 10.1007/978-1-4939-6817-6_9.http://myresearchnews.topic-debate.com/t5814-manipulating-gene-expression-in-the-chick-embryo
Snail2 and Zeb2 repress P-Cadherin to define embryonic territories in the chick embryo
Acloque H, Ocaña OH, Abad D, Stern CD, Nieto MA. Snail2 and Zeb2 repress P-Cadherin to define embryonic territories in the chick embryo. Development. 2017 Jan 1http://myresearchnews.topic-debate.com/t5721-snail2-and-zeb2-repress-p-cadherin-to-define-embryonic-territories-in-the-chick-embryo
Chick Development | ClipArt ETC
Origin of lungs, liver, and pancreas in the chick. The mesoderm is shaded; the endoderm dark. lg., One of the lungs; St., stomach; l., liver; p., pancreas." -Thomson, 1916. ...http://etc.usf.edu/clipart/48000/48029/48029_chick_deve.htm
13 day chicks with tumors? or funny growth quirk? | BackYard Chickens
Hi all. Thanks for all the help last weekend with my little Ameraucana--she's now doing fine! I noticed today that 3 of my chicks have a lump about...https://www.backyardchickens.com/threads/13-day-chicks-with-tumors-or-funny-growth-quirk.350726/