The protein migrates as 95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Cynomolgus LAG-3, ... is expressed from human 293 cells (HEK293). It contains AA Ala 18 - His 449 (Accession # XP_005570011.1).;This protein carries ...
10010-023) and heat immobilized at 82 °C for 2 min. Next, the cells were rinsed with PBS and digested using pepsin at 37 °C for ... 2012), cell inactivation (Kaigala et al. 2011), local lysis of cells for expression analysis and cell patterning (Kashyap et al ... Cells were seeded in chamber slides (Thermo Fisher Scientific, Cat. No. 10717931) at a density of 105 -106 cells cm−2. After 2- ... As a the model system, we used an immobilized MCF-7 cell monolayer and the centromeric FISH probes (satellite enumeration ...
Cell Material. Titanium coated with platinum black, plastic body. The actual cell constants are within ±10% of the nominal ... Plastic body, Titanium/Platinum black, k=1, conductivity cell with integrated temperature sensor ...
First, phospholipids are physically immobilized by high-pressure freezing and carbon/platinum replication. In addition, since ... Cell culture, transfection, and high-pressure freezing of HEK293 cells. The cDNA pCAGG PM-FRB-mRFP-T2A-FKBR-5-ptase was ... 9C). These results suggest the ubiquitous association of CaV2.1 with PI(4,5)P2 in neuronal cell membranes across various cell ... PI(4,5)P2 makes clusters on neuronal cell membranes in mouse cerebellum. How PI(4,5)P2 is distributed across cell membrane ( ...
2012). Immobilized metal affinity chromatography co-purifies TGF-beta1 with histidine-tagged recombinant extracellular proteins ... to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo ... 2015). Fibrillin-containing microfibrils are key signal relay stations for cell function. J Cell Commun Signal 9: 309-325. ... We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells ...
Almeida, C.; Brányik, T.; Moradas-Ferreira, P.; Teixeira, J. Continuous production of pectinase by immobilized yeast cells on ... This unit is connected to the surface of the cell by means of a type II cohesin-dockerin interaction. This makes this enzyme- ... 1,3-1,4-β-glucans are polysaccharides made up of glucose units that are found in endosperm cell walls of cereals such as barley ... Doi, R.H.; Kosugi, A. Cellulosomes: Plant-cell-wall-degrading enzyme complexes. Nat. Rev. Microbiol. 2004, 2, 541-551. [Google ...
by accumulating the cells at G0/G1 stage and reduced cell mobility. Oddly enough, silencing Kir2.1 increased the cell migration ... These were situated in sternal recumbency with the top immobilized within a headrest through padded supports and straps. * Post ... C-kit, also known as CD117 or stem cell growth factor, is the cell surface marker that has been used for stem cell isolation ... C-kit, also known as CD117 or stem cell growth factor, is the cell surface marker that has been used for stem cell isolation ...
White blood cell count was significantly reduced at the end of the 2nd treatment (13,330 ± 7,002 to 9,426 ± 5,188 /mm(3), p, ... Polymyxin B-immobilized fiber column (PMX) treatment for idiopathic pulmonary fibrosis with acute exacerbation: a multicenter ... Several clinical studies suggest that direct hemoperfusion with polymyxin B-immobilized fiber (PMX) may have beneficial effects ...
The continuous production of ethanol from beet molasses by Ca-alginate immobilized Saccharomyces cerevisiae in a packed-bed ... GÖKSUNGUR, YEKTA and ZORLU, NEŞE (2001) "Production of Ethanol from Beet Molasses by Ca-Alginate Immobilized Yeast Cells in a ... Production of Ethanol from Beet Molasses by Ca-Alginate Immobilized Yeast Cells in a Packed-Bed Bioreactor ... The continuous production of ethanol from beet molasses by Ca-alginate immobilized Saccharomyces cerevisiae in a packed-bed ...
Galactose derivative immobilized glow discharge processed polyethersulfone membranes maintain the liver cell metabolic activity ... Bioreactor Technology for Stem Cell Bioprocessing.. *Mesenchymal Stem Cells, Dental Pulp Stem Cells in vitro and in vivo for ... Embryonic stem (ES) cells/ human ES cells (hESCs) & human induced pluripotent stem cells (hIPs). ... Research Topic/Area: Endothelial cell differentiation from embryonic stem cells and adult stem cells. ...
Supercharged Cells has been renamed to High Velocity Supercharged Cells. The duration of High Velocity Supercharged Cells has ... Terminator Droid - Fires off a Terminator Droid to scan the area for 15, revealing stealthed opponents and immobilizing them ... Random Charge has been renamed Overcharged Cells. *Overcharged Cells - Priming Shot, Thermal Detonator, and Healing Scan ... Random Charge has been renamed Overcharged Cells. *Overcharged Cells - Vortex Bolt, Assault Plastique, and Advanced Medical ...
Cuvinte cheie: biotechnology, microbiology, immobilized microbial cells. *MARIAN PETRE. *iunie 12, 2006 ...
NRRL B-3805 cells immobilized in several Celite supports, BioTrans´99 * 1997, Influence of some operational parameters on the ... 1998, Whole-cell biocatalysis in organic media, Enzyme and Microbial Technology * 1998, Stability of free and immobilized ... 2002, Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media, ... NRRL B-3805 cells immobilized on Celite supports, Journal of Molecular Catalysis - B Enzymatic ...
A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. Therefore, ex vivo NK cell ... A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. Therefore, ex vivo NK cell ... Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and ... Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and ...
Sample Types: Whole Cell. Applications: Flow Cytometry * Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. ... Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1. Ala27-Thr343 (Thr130Ile). Accession # Q5JSL4 ... Engineered IL13 variants direct specificity of IL13Ralpha2-targeted CAR T cell therapy Authors: LA Stern, S Gholamin, I Moraga ...
Modulation of selectin-mediated adhesion of flowing lymphoma and bone marrow cells by immobilized SDF-1.﻽. Hedges EA, Hughes AD ... Stem cell factor and stromal cell co-culture prevent apoptosis in a subculture of the megakaryoblastic cell line, UT-7.﻽. ... This blood contains high concentrations of stem cells (cells from which all blood cells develop). This study is a protocol for ... Delivery of apoptotic signal to rolling cancer cells: a novel biomimetic technique using immobilized TRAIL and E-selectin.﻽. ...
This review describes commonly used protein markers of circulating tumor cells and provides an overview of the wide variety of ... The secreted proteins are therefore immobilized around the CTC which produce them and forms a spot around it. After the ... Hematopoietic cells. [56-61]. CD133. Stem cells from hematopoietic cells, brain, prostate liver, pancreas, lung colon and ovary ... Examples of cell/tissue types which express this protein. Ref.. EpCAM (also called HEA or BerEP4). Epithelial cells such as ...
The head, neck, and shoulders were immobilized using a thermoplastic mask (Posicast; Sinmed) prepared during a radiotherapy ... FDG-PET/CT imaging for preradiotherapy staging of head-and-neck squamous cell carcinoma. Int J Radiat Oncol Biol Phys. 2005;61: ... There were 13 male and 2 female patients, with a median age of 57 y (range, 46-61 y). The patients had squamous cell carcinoma ... Head and neck squamous cell carcinoma: value of diffusion-weighted MR imaging for nodal staging. Radiology. 2009;251:131-146. ...
Three proteins were immobilized, basic fibroblast growth factor (bFGF), cytochrome c, and insulin. BHK21 cells in buffer were ... As Figure 3 shows, the cells bound strongly to bFGF, a ligand for which receptors are abundant on the cell surface. The cells ... profiling drug-absorption characteristics on cell membrane arrays; and profiling cell-surface receptors on ligand arrays. In ... Cell-Receptor Profiling. No dextran gels or other matrices are used on SpotReady or SPRchip substrates, in order to provide ...
Electrolyte immobilized in gel. - Each cell has one-way safety pressure valve. - Safety fireproof equipment ...
Automated high-throughput analysis of B cell spreading on immobilized antibodies with whole slide imaging ... out of the snowflea Hypogastrura harveyi as a cryoprotectant in the cryopreservation of mesenchymal stem cells ...
By cross-linking bacterial surface proteins to fibrinogen, factor XIIIa causes bacteria to be immobilized and killed. Wound ... and endothelial cell receptors αvβ3 integrin and VEGFR-2. These observations have suggested physiologic and pathologic roles ...
This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material ... 1X Cell Extraction Buffer: Prepare by diluting FastScan™ ELISA Cell Extraction Buffer (5X) #69905 and FastScan™ ELISA Cell ... Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.. FastScan™ ELISA is a registered trademark of Cell ... FastScan™ ELISA Cell Extraction Buffer (5X) 69905 1 x 10 ml. FastScan™ ELISA Cell Extraction Enhancer Solution (50X) 25243 1 x ...
Encapsulation of sperm cells is a documented technology, and recently, a technology in which sperm cells are embedded in ... Use of immobilized cryopreserved bovine semen in a blind artifical insemination trial Abstract. To make timing of artificial ... In conclusion, this study has showed that immobilized spermatozoa provide equal fertility results as standard processed semen ... iodide and Alexa 488-conjugated peanut agglutinin to assess the proportion of plasma membrane and acrosome-intact sperm cells, ...
STUDY OF GENE EXPRESSION IN EUKARYOTE CELLS, UNDERSTAND... ... bacterial and streptomycete cells. A system using immobilized ... STUDY OF GENE EXPRESSION IN EUKARYOTE CELLS, UNDERSTANDING AND IMPROVED DIAGNOSIS OF DISEASE-RELATED PROTEIN ABNORMALITIES. 1- ... biotechnologically important bacteria and eucaryotic cells provides methods for screening and identifying bacterial strains, ... of 2-dimensional gel analysis namely PDQUEST and BioImage were implemented and databases were established for the studied cell ...
Research: Stem Cell and Cancer Biology. Dr. Lins research is focused on the stem cell and cancer biology, which have potential ... Examples of such endeavors include: modification of material surfaces with immobilized, bioactive compounds such as select ... Research: Neural Stem Cell Biology. The Hsieh laboratory is a neural stem cell biology laboratory that focuses on 4 major areas ... To clarify cell-biomaterial interactions we examine the integrin receptor activity of cells during their first contact with a ...