Hemoglobin transition in erythrocytes of developing chick. Studies with cell-free protein-synthesizing systems<...Hemoglobin transition in erythrocytes of developing chick. Studies with cell-free protein-synthesizing systems<...

Studies with cell-free protein-synthesizing systems",. abstract = "Cell-free hemoglobin-synthesizing systems from erythrocytes ... Cell-free hemoglobin-synthesizing systems from erythrocytes of 4- and 17-day chick embryos have been developed. These systems ... Cell-free hemoglobin-synthesizing systems from erythrocytes of 4- and 17-day chick embryos have been developed. These systems ... Cell-free hemoglobin-synthesizing systems from erythrocytes of 4- and 17-day chick embryos have been developed. These systems ...
more infohttps://scholars.uthscsa.edu/en/publications/hemoglobin-transition-in-erythrocytes-of-developing-chick-studies

Characterization of cell free synthesis of collagen by lung polysomes in a heterologous system<...Characterization of cell free synthesis of collagen by lung polysomes in a heterologous system<...

The cell free system was optimized with respect to K+, Mg2+, amino acids, and ribosomal wash fraction and used under conditions ... Characterization of cell free synthesis of collagen by lung polysomes in a heterologous system. / Collins, J. F.; Crystal, ... Characterization of cell free synthesis of collagen by lung polysomes in a heterologous system. Journal of Biological Chemistry ... Characterization of cell free synthesis of collagen by lung polysomes in a heterologous system. In: Journal of Biological ...
more infohttps://qfrd.pure.elsevier.com/en/publications/characterization-of-cell-free-synthesis-of-collagen-by-lung-polys

High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free...High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free...

... we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to ... we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to ... production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system ... production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system N ...
more infohttp://pubs.rsc.org/en/content/articlelanding/2016/mb/c6mb00209a

Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs | BMC Biotechnology...Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs | BMC Biotechnology...

The modified cell-free system combined with NLP offers the unique opportunity to produce sufficient expression levels of ... "Bmax cell-free expressed receptor/Bmax insect cell-expressed receptor × yield cell-free expressed receptor", assuming the ... Cell-Free Protein Expression Reactions. Cell-free protein expression was conducted using the MembraneMax™ Protein Expression ... In order to overcome these difficulties, we developed a cell-free expression system supplemented with planar membranes [15]. ...
more infohttps://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-57

Chemical Strategies to Enrich Malarial Proteins from Wheat LysateChemical Strategies to Enrich Malarial Proteins from Wheat Lysate

... schemes were developed that covalently attach a small molecule to the proteins in the wheat cell-free expression system. After ... Results from protein arrays can be affected by the sample purity, as high amounts of protein from the translation system can ... The results would be improved by separating the malarial protein from the translation system proteins. However, purifying the ...
more infohttps://digital.lib.washington.edu/researchworks/handle/1773/20610

Protein synthesis machinery from bacterial consortia in one shot | EurekAlert! Science NewsProtein synthesis machinery from bacterial consortia in one shot | EurekAlert! Science News

... developed at UC Davis may have broken the barrier to rapid assembly of pure protein synthesis machinery outside of living cells ... pure cell-free transcription-translation systems and throughput of studying disease-relevant pathways outside of living cells ... making it a potential game changer for high-throughput cell-free applications. ... purity reconstitution of the cellular reactions is critical for the high-throughput study of cellular pathways and cell-free ...
more infohttps://www.eurekalert.org/pub_releases/2017-11/uoc--psm111517.php

Cell-free system - WikipediaCell-free system - Wikipedia

A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full ... where a cell-free translation system based on Escherichia coli (E. coli), of the cell extract-based type, had the mRNA template ... Cell-free systems may be divided into two primary classifications: cell extract-based, which remove components from within a ... Notably, in work leading to a Nobel prize the Nirenberg and Matthaei experiment used a cell-free system, of the cell extract- ...
more infohttps://en.wikipedia.org/wiki/Cell-free_system

James Swartz on Cell-Free Systems | AIChEJames Swartz on Cell-Free Systems | AIChE

... and get a preview of his upcoming talk at the Cell Free Systems Conference. ... Hear from James about promising career paths in cell-free systems, ... The Cell Free Systems Conference will focus on understanding, harnessing, and expanding the capabilities of biological systems ... What topic will you be addressing at the Cell Free Systems Conference?. I will describe how we are using the new process and ...
more infohttps://www.aiche.org/chenected/2019/10/james-swartz-on-cell-free-systems

CRISPRs Unwanted Edits Unmasked Using Faster Cell Free SystemCRISPR's Unwanted Edits Unmasked Using Faster Cell Free System

... "cell-free" system eliminated a lot of the complex biological activity within a cell that makes it hard to isolate the full ... CRISPRs Unwanted Edits Unmasked Using Faster Cell Free System. December 9, 2019. 0 ... it frees scientists to execute and screen multiple trial edits, and many more than is practical with a cell-based system. This ... which is pioneering advances in editing DNA plasmids extracted from human cells. The team has already has used the cell-free ...
more infohttps://www.genengnews.com/news/crisprs-unwanted-edits-unmasked-using-faster-cell-free-system/

Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle | ScienceEnhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle | Science

Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ... Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ... Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ... Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ...
more infohttp://science.sciencemag.org/content/157/3791/935

Human immunodeficiency virus integration in a cell-free system. | Journal of VirologyHuman immunodeficiency virus integration in a cell-free system. | Journal of Virology

Human immunodeficiency virus integration in a cell-free system.. V Ellison, H Abrams, T Roe, J Lifson, P Brown ... Human immunodeficiency virus integration in a cell-free system. Message Subject (Your Name) has forwarded a page to you from ... Integration of the viral genome into the nuclear DNA of a host cell plays a pivotal role in the replication of retroviruses. We ... integration by using extracts from HIV-infected cells. Analysis of the reaction products showed that HIV integration in vitro ...
more infohttps://jvi.asm.org/content/64/6/2711?ijkey=8a6824077b0857d4ad40a6b784b4b73f38a41bf0&keytype2=tf_ipsecsha

Cytokinesis signaling visualized in vitro, using Xenopus cell-free system
	Cytokinesis signaling visualized in vitro, using Xenopus cell-free system

Cytokinesis signaling visualized in vitro, using Xenopus cell-free system. Cytokinesis, when two daughter cells are physically ... have reconstituted the organization of cytokinesis signaling outside living cells, using a the cell-free Xenopus system. The ... We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules ... Spatial organization of cytokinesis signaling reconstituted in a cell-free system. Phuong A. Nguyen et al. Science 346, 244 ( ...
more infohttp://www.xenbase.org/entry/doNewsRead.do?id=167

Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins (Patent) | DOepatentsCell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins (Patent) | DOepatents

Title: Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins. ... Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins ... A low power EM sensor is used to detect the motions of windpipe tissues in the glottal region of the human speech system before ... A low power EM sensor is used to detect the motions of windpipe tissues in the glottal region of the human speech system before ...
more infohttps://www.osti.gov/doepatents/biblio/1084360-cell-free-system-synthesizing-membrane-proteins-cell-free-method-synthesizing-membrane-proteins

Susceptibility of influenza viruses to hypothiocyanite and hypoiodite produced by lactoperoxidase in a cell-free systemSusceptibility of influenza viruses to hypothiocyanite and hypoiodite produced by lactoperoxidase in a cell-free system

The results presented here show that the LPO/H2O2/(SCN-/I-) cell-free, in vitro experimental system is a functional tool to ... Our aim was to study the antiviral effect and substrate specificity of LPO to inactivate influenza viruses using a cell-free ... We propose that a LPO-based antiviral system is an important contributor to anti-influenza virus defense of the airways. ... These studies tested the hypothesis that influenza strains are all susceptible to the LPO-based antiviral system but exhibit ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199167

Multi-Input Regulation and Logic with T7 Promoters in Cells and Cell-Free SystemsMulti-Input Regulation and Logic with T7 Promoters in Cells and Cell-Free Systems

... coli cell-free protein expression system. Our results expand the utility of T7 promoters in cell based as well as cell-free ... offer a simple orthogonal expression system for use in a variety of cellular backgrounds and even in cell-free systems. Here we ... two different transcriptional repressors for the construction of a logic gate that functions in cells and in cell-free systems ... Engineered gene circuits offer an opportunity to harness biological systems for biotechnological and biomedical applications. ...
more infohttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0078442

Biosynthesis of heparin : Modulation of polysaccharide chain length in a cell-free systemBiosynthesis of heparin : Modulation of polysaccharide chain length in a cell-free system

Biosynthesis of heparin: Modulation of polysaccharide chain length in a cell-free system. Lidholt, Kerstin Uppsala University, ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:455588

Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset...Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset...

... which was suitable for wheat germ cell-free protein synthesis [20]. These results suggested that the wheat germ cell-free ... N. Kaneko, Y. Ito, T. Iwasaki et al., "Reconstituted AIM2 inflammasome in cell-free system," Journal of Immunological Methods, ... It is noted that there are some limitations of nodosome in a cell-free system. Only the initial event of Nod2-RICK interaction ... MDP-Induced Interaction between Nod2 and RICK in Nodosome in a Cell-Free System. Nod2-WT-Btn was coprecipitated with FLAG-RICK- ...
more infohttps://www.hindawi.com/journals/tswj/2016/2597376/

Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free systemLinear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system

... Zachary ... protect linear DNA strands from exonuclease degradation in an Escherichia coli based transcription-translation cell-free system ...
more infohttp://www.cds.caltech.edu/~murray/papers/sun+13-acs_synbio.html

Molecules | Free Full-Text | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human...Molecules | Free Full-Text | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human...

The aim of this study was to investigate the antioxidant activity of Ixora parviflora extract (IPE) in a cell-free system and ... Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. Kuo-Ching Wen 1, Hua- ... "Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts." Molecules 16, no. 7: ... Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. Molecules 2011, 16, ...
more infohttp://www.mdpi.com/1420-3049/16/7/5735

Difference between revisions of Establishing microfluidic cell-free systems for the rapid prototyping of synthetic genetic...Difference between revisions of "Establishing microfluidic cell-free systems for the rapid prototyping of synthetic genetic...

This study is designed to demonstrate that biological systems can be forward and reverse engineered in cell-free environments. ... Difference between revisions of "Establishing microfluidic cell-free systems for the rapid prototyping of synthetic genetic ... cell-free_systems_for_the_rapid_prototyping_of_synthetic_genetic_networks&oldid=22493" ... We propose to develop a closed feedback system that controls the microfluidic system, runs experiments and analyses results to ...
more infohttp://www.cds.caltech.edu/~murray/wiki/index.php?title=Establishing_microfluidic_cell-free_systems_for_the_rapid_prototyping_of_synthetic_genetic_networks&diff=22493&oldid=18429

Frontiers | Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza...Frontiers | Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza...

A recombinant full-length HPIV3-HN was successfully synthesized using the wheat-germ cell-free protein production system. After ... After immunization and cell fusion, 36 mouse hybridomas producing MAbs to HPIV3-HN were established. The MAbs obtained were ... immunization and cell fusion, 36 mouse hybridomas producing MAbs to HPIV3-HN were established. The MAbs obtained were fully ... A recombinant full-length HPIV3-HN was successfully synthesized using the wheat-germ cell-free protein production system. ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2014.00208/full

Disassembly of the Drosophila nuclear lamina in a homologous cell-free system | Journal of Cell ScienceDisassembly of the Drosophila nuclear lamina in a homologous cell-free system | Journal of Cell Science

This system will allow enzymological characterization of cell-free lamina disassembly as well as molecular analysis of specific ... Disassembly of the Drosophila nuclear lamina in a homologous cell-free system ... Disassembly of the Drosophila nuclear lamina in a homologous cell-free system ... Disassembly of the Drosophila nuclear lamina in a homologous cell-free system ...
more infohttp://jcs.biologists.org/content/108/5/2027

Lirias: The wheat germ cell-free system possesses processing activity for the precursor of human placental lactogenLirias: The wheat germ cell-free system possesses processing activity for the precursor of human placental lactogen

5. The presence of one or several processing enzymes in the wheat germ cell-free system was indicated by the effect of Triton X ... The wheat germ cell-free system possesses processing activity for the precursor of human placental lactogen. ... The poly(A)-containing fraction stimulated amino acid incorporation 5- to 10-fold in the wheat germ cell-free system. ... Education Domains Department of Molecular Cell Biology (-) › Biochemistry Section (Medicine) (-) › ...
more infohttps://lirias.kuleuven.be/handle/123456789/11410

KAKEN - Research Projects | Study on initiation of DNA repication in cell-free system of Xenopus eggs (KAKENHI-PROJECT-07680762)KAKEN - Research Projects | Study on initiation of DNA repication in cell-free system of Xenopus eggs (KAKENHI-PROJECT-07680762)

Study on initiation of DNA repication in cell-free system of Xenopus eggs. Research Project ... which has an activity to untwist DNA and open double strand DNA to single strand DNA from cell-free DNA synthetic system of ... Publications] Someya, A.: The association and involvement of the P1 protein family in a cell free extract of Xenopus eggs ... Publications] Someya.A.: The possible involvement of replication-related proteins with a DEAD-box-like motif in cell-free DNA ...
more infohttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680762/

An ABCB10 cell-free system and the exploration of its substrates and regulatorsAn ABCB10 cell-free system and the exploration of its substrates and regulators

... ... In this dissertation an 8-azido-ATP photolabeling system is established to study the ATP binding and hydrolysis properties of ... Therefore, the 8-azido-ATP photolabeling system can be utilized to test potential substrates of ABCB10. Substances related to ... The 8-azido-ATP photolabeling system shows that GSSG stimulates ATP hydrolysis without affecting ATP binding, whereas GSH ...
more infohttps://open.bu.edu/handle/2144/15368