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*  A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins
Developing a cell-free system based on cultured cells for the synthesis of membrane proteins could provide a highly ... These results showed that the newly developed cell-free translocation system derived from cultured insect cells is a practical ... A transporter protein synthesized with the cell-free system could be functionally reconstituted into a lipid bilayer. In ... procedure and evaluated the performance of the translocation system in combination with a cell-free translation system ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112874
*  High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free...
... we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to ... we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to ... production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system ... production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system N ...
http://pubs.rsc.org/en/content/articlelanding/2016/mb/c6mb00209a
*  Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs | BMC Biotechnology...
The modified cell-free system combined with NLP offers the unique opportunity to produce sufficient expression levels of ... "Bmax cell-free expressed receptor/Bmax insect cell-expressed receptor × yield cell-free expressed receptor", assuming the ... Cell-Free Protein Expression Reactions. Cell-free protein expression was conducted using the MembraneMax™ Protein Expression ... In order to overcome these difficulties, we developed a cell-free expression system supplemented with planar membranes [15]. ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-57
*  Chemical Strategies to Enrich Malarial Proteins from Wheat Lysate
... schemes were developed that covalently attach a small molecule to the proteins in the wheat cell-free expression system. After ... Results from protein arrays can be affected by the sample purity, as high amounts of protein from the translation system can ... The results would be improved by separating the malarial protein from the translation system proteins. However, purifying the ...
https://digital.lib.washington.edu/researchworks/handle/1773/20610
*  J-STORE(~METHOD FOR ANALYZING PROTEINS CONTRIBUTING TO AUTOIMMUNE DISEASES, AND METHOD FOR TESTING FOR SAID DISEASES SAWASAKI,...
... which are expressed by a cell-free protein synthesis system, into contact with samples derived from patients with autoimmune ... which are expressed by a cell-free protein synthesis system, into contact with samples derived from patients with autoimmune ...
https://jstore.jst.go.jp/foreignPatentDetail.html?foreign_id=6747
*  Cell-free system - Wikipedia
A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full ... where a cell-free translation system based on Escherichia coli (E. coli), of the cell extract-based type, had the mRNA template ... Cell-free systems may be divided into two primary classifications: cell extract-based, which remove components from within a ... Notably, in work leading to a Nobel prize the Nirenberg and Matthaei experiment used a cell-free system, of the cell extract- ...
https://en.wikipedia.org/wiki/Cell-free_system
*  Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle | Science
Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ... Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ... Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ... Enhanced Protein Synthesis in a Cell-Free System from Hypertrophied Skeletal Muscle ...
http://science.sciencemag.org/content/157/3791/935
*  Cytokinesis signaling visualized in vitro, using Xenopus cell-free system
Cytokinesis signaling visualized in vitro, using Xenopus cell-free system. Cytokinesis, when two daughter cells are physically ... have reconstituted the organization of cytokinesis signaling outside living cells, using a the cell-free Xenopus system. The ... We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules ... Spatial organization of cytokinesis signaling reconstituted in a cell-free system. Phuong A. Nguyen et al. Science 346, 244 ( ...
http://www.xenbase.org/entry/doNewsRead.do?id=167
*  Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins (Patent) | DOepatents
Title: Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins. ... Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins ... A low power EM sensor is used to detect the motions of windpipe tissues in the glottal region of the human speech system before ... A low power EM sensor is used to detect the motions of windpipe tissues in the glottal region of the human speech system before ...
https://www.osti.gov/doepatents/biblio/1084360-cell-free-system-synthesizing-membrane-proteins-cell-free-method-synthesizing-membrane-proteins
*  Multi-Input Regulation and Logic with T7 Promoters in Cells and Cell-Free Systems
... coli cell-free protein expression system. Our results expand the utility of T7 promoters in cell based as well as cell-free ... offer a simple orthogonal expression system for use in a variety of cellular backgrounds and even in cell-free systems. Here we ... two different transcriptional repressors for the construction of a logic gate that functions in cells and in cell-free systems ... Engineered gene circuits offer an opportunity to harness biological systems for biotechnological and biomedical applications. ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0078442
*  Biosynthesis of heparin : Modulation of polysaccharide chain length in a cell-free system
Biosynthesis of heparin: Modulation of polysaccharide chain length in a cell-free system. Lidholt, Kerstin Uppsala University, ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:455588
*  Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset...
... which was suitable for wheat germ cell-free protein synthesis [20]. These results suggested that the wheat germ cell-free ... N. Kaneko, Y. Ito, T. Iwasaki et al., "Reconstituted AIM2 inflammasome in cell-free system," Journal of Immunological Methods, ... It is noted that there are some limitations of nodosome in a cell-free system. Only the initial event of Nod2-RICK interaction ... MDP-Induced Interaction between Nod2 and RICK in Nodosome in a Cell-Free System. Nod2-WT-Btn was coprecipitated with FLAG-RICK- ...
https://www.hindawi.com/journals/tswj/2016/2597376/
*  Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system
... Zachary ... protect linear DNA strands from exonuclease degradation in an Escherichia coli based transcription-translation cell-free system ...
http://www.cds.caltech.edu/~murray/papers/sun+13-acs_synbio.html
*  Molecules | Free Full-Text | Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human...
The aim of this study was to investigate the antioxidant activity of Ixora parviflora extract (IPE) in a cell-free system and ... Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. Kuo-Ching Wen 1, Hua- ... "Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts." Molecules 16, no. 7: ... Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. Molecules 2011, 16, ...
http://www.mdpi.com/1420-3049/16/7/5735
*  Frontiers | Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza...
A recombinant full-length HPIV3-HN was successfully synthesized using the wheat-germ cell-free protein production system. After ... After immunization and cell fusion, 36 mouse hybridomas producing MAbs to HPIV3-HN were established. The MAbs obtained were ... immunization and cell fusion, 36 mouse hybridomas producing MAbs to HPIV3-HN were established. The MAbs obtained were fully ... A recombinant full-length HPIV3-HN was successfully synthesized using the wheat-germ cell-free protein production system. ...
https://www.frontiersin.org/articles/10.3389/fmicb.2014.00208/full
*  Disassembly of the Drosophila nuclear lamina in a homologous cell-free system | Journal of Cell Science
This system will allow enzymological characterization of cell-free lamina disassembly as well as molecular analysis of specific ... Disassembly of the Drosophila nuclear lamina in a homologous cell-free system ... Disassembly of the Drosophila nuclear lamina in a homologous cell-free system ... Disassembly of the Drosophila nuclear lamina in a homologous cell-free system ...
http://jcs.biologists.org/content/108/5/2027
*  Lirias: The wheat germ cell-free system possesses processing activity for the precursor of human placental lactogen
5. The presence of one or several processing enzymes in the wheat germ cell-free system was indicated by the effect of Triton X ... The wheat germ cell-free system possesses processing activity for the precursor of human placental lactogen. ... The poly(A)-containing fraction stimulated amino acid incorporation 5- to 10-fold in the wheat germ cell-free system. ... Education Domains Department of Molecular Cell Biology (-) › Biochemistry Section (Medicine) (-) › ...
https://lirias.kuleuven.be/handle/123456789/11410
*  KAKEN - Research Projects | Study on initiation of DNA repication in cell-free system of Xenopus eggs (KAKENHI-PROJECT-07680762)
Study on initiation of DNA repication in cell-free system of Xenopus eggs. Research Project ... which has an activity to untwist DNA and open double strand DNA to single strand DNA from cell-free DNA synthetic system of ... Publications] Someya, A.: 'The association and involvement of the P1 protein family in a cell free extract of Xenopus eggs' ... Publications] Someya.A.: 'The possible involvement of replication-related proteins with a DEAD-box-like motif in cell-free DNA ...
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680762/
*  An ABCB10 cell-free system and the exploration of its substrates and regulators
... ... In this dissertation an 8-azido-ATP photolabeling system is established to study the ATP binding and hydrolysis properties of ... Therefore, the 8-azido-ATP photolabeling system can be utilized to test potential substrates of ABCB10. Substances related to ... The 8-azido-ATP photolabeling system shows that GSSG stimulates ATP hydrolysis without affecting ATP binding, whereas GSH ...
https://open.bu.edu/handle/2144/15368
*  Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems - Galeffi, Patrizia, Lombardi,...
... but a worldwide deficit in the manufacturing capacities of mammalian cell ... Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems : Aberrant signaling by ErbB-2 (HER ... Research Open Access Functional expression of a single-cha in antibody to ErbB-2 in plants and cell-free systems Patrizia ... Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems Galeffi, Patrizia, Lombardi, Alessio ...
https://www.youscribe.com/catalogue/documents/savoirs/functional-expression-of-a-single-chain-antibody-to-erbb-2-in-plants-2059553
*  Cytoplasmic components of apoptosis by irofulven revealed by cell-free apoptotic systems | Cancer Research
Cytoplasmic components of apoptosis by irofulven revealed by cell-free apoptotic systems. Jan M. Woynarowski, Barbara A. ... Cytoplasmic components of apoptosis by irofulven revealed by cell-free apoptotic systems ... To better understand the nature of the differential responses of tumor and normal cells, we used a cell-free apoptosis ... Cytoplasmic components of apoptosis by irofulven revealed by cell-free apoptotic systems ...
http://cancerres.aacrjournals.org/content/64/7_Supplement/1195.4
*  Recombination between adenovirus type 12 DNA and a hamster preinsertion sequence in a cell-free system. Patch homologies and...
We have previously described a cell-free recombination system derived from hamster cell nuclear extracts in which the in vitro ... Further work will be required to ascertain that the cell-free recombination system mimics certain elements of the mechanisms of ... Recombination between adenovirus type 12 DNA and a hamster preinsertion sequence in a cell-free system. Patch homologies and ... Recombination between adenovirus type 12 DNA and a hamster preinsertion sequence in a cell-free system. Patch homologies and ...
http://www.biochemie.abi.med.uni-muenchen.de/publications/article/pub_1992_007.html
*  IUCr) Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline
The wheat germ cell-free expression system combines the advantages of cell-free and eukaryotic systems and is well suited for ... coli expressed soluble protein in the cell-free system (Table 1. ). These data demonstrate that the wheat germ cell-free ... Cell-free expression. Cell-free solubility. Anaplasma marginale. ACR67103.1. Full length. Major surface protein 2 variant 9H1. ... Prokaryotic cell-free systems exist, although these systems have demonstrated limited improvement over prokaryotic in vivo ...
http://journals.iucr.org/f/issues/2011/09/00/en5477/index.html
*  Effect of some uracil analogues on amino acid incorporation into a liver cell-free system
... ... Effect of some uracil analogues on amino acid incorporation into a liver cell-free system, Voprosy meditsinskoi khimii, 1969, ...
http://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-1969-15-4-393-en
*  A Cell-Free Assay System for β-Catenin Signaling That Recapitulates Direct Inductive Events in the Early Xenopus laevis Embryo ...
This cell-free assay system offers several clear advantages over cleaving embryos as a system for identification of direct ... Cell-free Assay for β-Catenin Signaling. A schematic diagram of the cell-free assay used in these studies is shown in Fig. 1. ... A Cell-Free Assay System for β-Catenin Signaling That Recapitulates Direct Inductive Events in the Early Xenopus laevis Embryo ... A cell-free assay system for β-catenin signaling. 40 min after fertilization, cleavage blockade of Xenopus embryos is brought ...
http://jcb.rupress.org/content/147/2/367?ijkey=d3e1da900189f9768ddef8f35c307e7d79328e1d&keytype2=tf_ipsecsha