... were evaluated for attachment to micro-patterned and un-patterned silicone surfaces in a short-term colonization assay. Two key ... is a leading hospital acquired infection in intensive care units despite improved patient care practices and advancements in ... and counting the colony forming units (CFU) after sufficient growth at 37°C overnight. Each CFU per sample data point was log10 ... van Kooten TG, von Recum AF: Cell adhesion to textured silicone surfaces: the influence of time of adhesion and texture on ...

Here we show that heat shock cognate protein 70 (Hsc70) contributes to bacterial uptake by TG cells and the EEVD motif of Hsc70 ... The recombinant TPR proteins were used for investigation of the effect of TPR proteins on bacterial uptake by TG cells and on ... The monoclonal antibody that inhibits bacterial uptake by TG cells reacted with the EEVD motif of Hsc70. Bacterial TPR proteins ... Our results demonstrate that surface located Hsc70 on TG cells mediates the uptake of pathogenic bacteria and proteins ...

0306]Colony forming unit (CFU) assays were used to examine the effects of Homspera® in stimulating hematopoietic stem cells ... Megakaryocyte-Colony Forming Cells (Mk-CFC), T-lymphocyte-Colony Forming Cells (T-CFC), B-lymphocyte-Colony Forming Cells (B- ... Megakaryocyte-Colony Forming Cells (Mk-CFC), T-lymphocyte-Colony Forming Cells (T-CFC), B-lymphocyte-Colony Forming Cells (B- ... Unit--Erythroid (BFU-E) cultures, Colony Forming Unit--Erythroid (CFU-E) cultures, and Colony Forming Unit--Granulocyte/ ...

The monomeric composition in the PHA formed was relatively uniform throughout incubation with 3-hydroxydecanoate dominating. ... When glycerin was added to the bioreactor (75 g/L total addition), the final cell dry weight (CDW) and PHA content were 30 g/L ... Cell Dry Weight and Colony Forming Unit Measurement Cell dry weights (CDW) were obtained by harvesting 30 ... Samples were assayed for nitrogen and phosphorus using Hach test kits and a Hach DR/2010 colorimeter (Hach, ...

Cells were allowed to grow and form colonies for several days and the number of colonies was counted. ... Unit 19.7 imaging tumor cell movement in vivo. Curr Protocol Cell Biol ... The in vivo invasion and intravasation assays were done as described previously (6, 10). ... Cells were treated as described in Materials and Methods, and colonies of AC480-treated cells were counted and plotted in ...

IL-4 suppresses granulocyte-macrophage colony-forming unit (CFU-GM) proliferation but it enhances the colony-stimulatory effect ... or peripheral blood cells from 30 patients using the CFU-granulocyte-erythrocyte-monocyte-megakaryocyte colony culture assay. ... IL-4 suppresses granulocyte-macrophage colony-forming unit (CFU-GM) proliferation but it enhances the colony-stimulatory effect ... IL-4 suppresses granulocyte-macrophage colony-forming unit (CFU-GM) proliferation but it enhances the colony-stimulatory effect ...

... it should not contain a total viable microbial count greater than 200 colony-forming units (CFU)/ml. The dialysis fluid should ... For example, cells of M. tuberculosis or M. bovis, which are used in routine efficacy tests are among the most resistant ... Appropriate microbiologic assays may be considered; however, the nearest district office of the FDA, local and state health ... The circumstances that require handwashing are frequently found in high-risk units, because patients in these units are often ...

... colony forming units mix (CFU-mix), and granulocyte-macrophage colony-forming units (CFU-GM), we used 7000 cells/ml. The ... of DP on the clonal growth of erythroid and granulocyte-macrophage progenitors were investigated using clonogenic assays ... For erythroid colony forming unit (CFU-E) quantification, we used 2000 cells/ml, while for erythroid burst forming units (BFU-E ... After colony enumeration, BFU-E and CFU-mix plates were washed and total cells counted using a cell analyser (Coulter ...

After 48 h cells were kept in RPMI-1640 with 10% FBS (Gibco) supplemented with 600 μg/ml G418 antibiotic. Once colonies formed ... Luciferase reporter assay. Luciferase assays were performed as per recommended standard protocol. Clonal cells from replica ... relative light units/μg/second); patterned bars represent clones with distinct NIS expression on the cell membrane, which was ... S4A-C). IF of NIS was done in cl31 and cl6 cells after treating cells with inhibitors for 48 h. The cl31 cells treated with DMM ...

Unless otherwise stated, the bacterial biomass of the cultured cells was determined based on the value of colony forming unit ( ... Detection of Reducing Sugar and Assay of -Amylase Activity. After fermentation, the cell mixture was centrifuged at 10,000 g ... Then, the cells were tenfold gradiently diluted. Finally, 100 μL of cells was spread to a culture medium agar plate for growth ... "Polymyxin E induces rapid Paenibacillus polymyxa death by damaging cell membrane while Ca2+ can protect cells from damage," ...

For quantification, Colony forming units (CFUs) are measured using traditional plate count methods, while flow cytometry​ ... VBNC cells cannot be quantified using the traditional, culture-dependent, plate count method, however, VBNC cells can reacquire ... "The method published is different as it follows the validation guidelines for both the identification and enumeration assays." ... measures Active Fluorescent Units (AFUs) and is an indicator of cell viability. ...

... occur as sessile cells. Infection induces an increase in the number of hemocytes, and tubulin and nuclear staining showed that ... and data show that some hemocytes also exist as sessile cells that are attached to tissues. The purpose of this study was to ... The primary regulators of these immune responses are cells called hemocytes, which kill pathogens via phagocytosis and via the ... this increase is primarily due to mitosis and, more specifically, autonomous cell division, by circulating granulocytes. The ...

The key difference between mesenchymal markers and stem cell markers is that mesenchymal markers originate from the multipotent ... to identify a subset of mesenchymal stem cells with high colony forming units or to identify embryonic stem cell-like cells. ... Even though functional assays act as an ideal approach for the identification of stem cells, the molecular markers or stem cell ... Stem cells are indefinite dividing cells that give rise to differentiated cell types. Stem cells are known to fill up or ...

... formed strong biofilms and exo-A (30.4%). No significant relation between motility, urease and catalase production with ... Students test was used for the statistical analysis and data are represented as the mean colony forming units (CFU ± SD) from ... it also interferes with cell respiration, calcium homeostasis and prostacyclin release from lung endothelial cells as well [21 ... In vitro microplate biofilm assay The ability of P. aeruginosa strains to develop biofilm was determined on 96-well microtiter ...

... plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process ... colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, ... the units refers to the viral particles and different units reflect the different assays involved. ... Immortalized Cell Lines. Stable Cell Lines. Tumor Cell Lines. Primary Cells. Mycoplasma Detection & Elimination. Media, ...

... most significantly in the post-thaw colony forming assay which indicates functionality of the stem cells for engraftment. ... Storing the cord blood unit in five smaller volumes makes it possible to use a portion of the stem cells and save the rest for ... carrying a specific drug to a specific cell or tissues2. The exosomes released by regenerative cells such as stem cells, are ... packed full of stem cells, red and white blood cells, even disease-fighting T-cells. It is important to take time to think ...

Similarly, the higher Rv3737 mRNA level resulted in lower C(t) value by Xpert MTB/RIF assay, demonstrating that a positive ... This strain was evaluated for altered growth rate and macrophage survival using a cell model of infection. In addition, the ... colony forming unit) were counted [14].. Msm/pMV261-Rv3737 and Msm/pMV261 were infected to 5 × 105/well RAW 264.7 cells in 6- ... colony forming unit) were counted.. Cytokine measurement. Culture supernatants and sediments from Mtb-infected RAW 264.7 cells ...

Immature leukaemic blast colony-forming units (AML-CFU), detected in in vitro cultures, were present in all donor samples ... Stem Cells 2000;18(3):157-65. 69Wu Braun S, Gerhartz HH, Schmetzer HM. Cytokines can reduce clonal, CD34-positive cells in ... Suspension culture assays may also be carried out in serum free medium with similar recombinant growth factors[38]. Duration of ... Self-renewal potential of LSC is demonstrable by colony forming blasts units (CFU-blast). CFUs were achieved by growing samples ...

The inhibition zones in ADT were measured after 48 h and the colony-forming units counting in the DCT after 1, 24, 72 and 168 h ... analysed on human gingival fibroblasts submitted to cell culture medium conditioned by sealers using the MTT reduction assay ... The inhibition zones in ADT were measured after 48 h and the colony-forming units counting in the DCT after 1, 24, 72 and 168 h ... The inhibition zones in ADT were measured after 48 h and the colony-forming units counting in the DCT after 1, 24, 72 and 168 h ...

Cell Migration Assays. *Cell Separation. *Cell Tracking. *Cellular Reprogramming Techniques. *Colony-Forming Units Assay ... CD40 Agonist Overcomes T Cell Exhaustion Induced by Chronic Myeloid Cell IL-27 Production in a Pancreatic Cancer Preclinical ... "Drug Screening Assays, Antitumor" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth ...

Cell injuries were confirmed by Fourier transform infrared spectroscopy, revealing that ZNGs antimicrobial effect was related ... revealed ZNGs mechanical interaction at cell wall level. ZNGs induced in those bacteria deep physical damages not compatible ... A time- and dose-dependent bactericidal effect in cell viability was highlighted against both bacteria, demonstrating a strong ... The ability of bacterial survival was assessed by the colony count method (Colony Forming Unit, CFU) for both types of tests, ...

Cell Tracking [E05.242.373] * Cellular Reprogramming Techniques [E05.242.378] * Colony-Forming Units Assay [E05.242.383] ... The manipulation of STEM CELL culture conditions to induce cell differentiation into a particular cell type.. Terms. Directed ... Cell Reprogramming Techniques Direct Cell Reprogramming Techniques Directed Differentiation Techniques Previous Indexing. Cell ... The creation of a pluripotent stem cell or other cell type from an adult somatic cell by the forced expression of specific ...

Colony forming assay. Soft agar colony formation assays were performed using the CytoSelect 96-well Cell Transformation Assay ... The relative luciferase activity was calculated as relative light units (RLU/β-galactosidase). ... D. The cell growth capacity of SNU484 cells transfected with miR-130a and miR-495 mimics (20 nM) was detected by colony ... Luciferase assay. For luciferase assays, HEK293 cells were plated into 35-mm dishes 1 day before transfection. Forty-eight ...

The LOD of this assay was 104 colony forming units (CFU)/mL, which was 100-occasions more sensitive than a traditional strip ... The principal glial cell of the retina is the Mller cell, expresses AQP4, and is enriched around the fovea.25 Mller cell bodies ... anti-B cell (Compact disc19) and anti-monocyte (UCHM1) antibodies at 10 g/ml to eliminate turned on T cells, B cells and ... The principal glial cell of the retina is the Mller cell, expresses AQP4, and is enriched around the fovea. * Post author By ...

The limit of detection (LoD) of Xpert MTB/RIF assay is 131 colony-forming units (CFU)/mL and the LoD for Xpert Ultra is 16 CFU/ ... CD8 + T-cells can improve performance in immunocompromised conditions that affect CD4+ T-cell responses (e.g., HIV) and improve ... Xpert MTB/extensively drug-resistant (XDR)® assay (Cepheid, USA). It is a novel rapid, cartridge-based assay that is intended ... Xpert MTB/RIF assay. The Xpert® MTB/RIF assay (Cepheid; Sunnyvale, CA, USA) amplifies nucleic acids through real-time ...

... assay. Klebsiella pneumoniae and Proteus mirabilis biofilms of initial broth suspensions of 108 colony forming units (CFU) per ... Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells. Blood Cells, Molecules, and Diseases. ... A proteomic approach to identify the integrins α 6 and α V as HPA binding partners in the metastatic colorectal cancer cell ... A proteomic approach to identify the integrins α 6 and α V as HPA binding partners in the metastatic colorectal cancer cell ...

To determine the number of colony forming unit (CFU), one sample was serially diluted and plated on Brain-Heart Infusion (BHI) ... Sporulation assay Mid-exponential growth cultures of C. difficile JIR8094 and its isogenic ccpA mutant in SM medium were used to ... Percentage of sporulation is the ratio between the spore titer and total cell titer at each time point. The mean and standard ... In Bacilli, the DNA-binding activity of CcpA is enhanced by its interaction with HPr-Ser-P, a phosphorylated form of HPr (16). ...

The sensitivity threshold for this PCR primer-based method was approximately 102-103 colony forming units (CFU) per reaction, ... The cells were collected by centrifugation and lysed in Cell Lysis solution 600 μl at 80 °C for 5 min (Qiagen, Tokyo, Japan). ... optimized this technique and developed a highly sensitive nested PCR assay [20]. The detection limit of the nested PCR method ... Several groups were formed based upon the subjects answers. (1) The smoking group was comprised of subjects smoking one ...