Here, we review the interplay between membrane components and the physical properties of the plasma membrane worth considering ... Later, we discuss the main advantages and caveats of different model membrane systems. We further expand on how the use of ... We discuss the similarities of immune synapses observed for innate and adaptive immune cells and focus on the physical ... model systems has contributed to the understanding of immune cell signaling, with a specific focus on the immunological synapse ...

Thus the site of action of the T cell factor was not on other T cells, but was either on macrophages or the B cells themselves ... hapten-primed cells yielded greater responses. The T cell factor acted as efficiently if T cells were present or absent in the ... The T cell-specific immunizing factor did not pass through dialysis membranes. The experiments reported here help rule out some ... Critical tests were performed to verify that the membranes used were in fact cell impermeable. The specificity of the ...

Plasma membrane Kv1.3 mediates cellular activation and proliferation, whereas mitochondrial Kv1.3 participates in cell survival ... Several forward-traffic motifs target the channel to the plasma membrane in a COPII-dependent manner. However, the ... Kv1.3 uses the TIM23 complex to translocate to the inner mitochondrial membrane. This mechanism is unconventional because the ... Our results provide insights into the mechanisms mediating the localization of Kv1.3 to mitochondrial membranes, further ...

Transforming growth factor alpha (TGF alpha) caused a more than threefold increase in synovial cell proliferation whilst ... Enhanced cell proliferation, glycolysis and prostaglandin E production are all characteristic features of rheumatoid synovial ... Synovial Membrane, Transforming Growth Factors ... caused a more than threefold increase in synovial cell ... Enhanced cell proliferation, glycolysis and prostaglandin E production are all characteristic features of rheumatoid synovial ...

The potential of formed scaffold structures for tissue engineering applications was demonstrated using mouse cell lines (L929 ... The potential of formed scaffold structures for tissue engineering applications was demonstrated using mouse cell lines (L929 ... Membranes, Mice, Microbubbles, Phthalic Acids, Serum Albumin, Bovine, Tissue Engineering, Tissue Scaffolds ... Animals, Biocompatible Materials, Biosensing Techniques, Catechols, Cell Line, Drug Delivery Systems, Ethanol, Hydrogen ...

... were performed in melanoma M2 cells expressing mutant forms of ezrin that provide a link between the plasma membrane and the ... Animals, Calibration, Cell Membrane, Fluorescence Recovery After Photobleaching, Image Processing, Computer-Assisted, Melanoma ... were performed in melanoma M2 cells expressing mutant forms of ezrin that provide a link between the plasma membrane and the ... Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching. ...

One of the critical proteinases involved in cell migration is membrane-type 1 matrix metalloproteinase (MT1-MMP/MMP-14). MT1- ... enhancing cell migration. For MT1-MMP to promote cell migration, it needs to act in co-ordination with other cell migration ... MMP degrades extracellular matrix to make a path for cells to migrate, sheds cell surface molecules to give migratory signals, ... However once control is lost, cell migration facilitates disease progression such as seen in cancer metastasis, atherosclerosis ...

Stromal cells play a central role in the transformation of an acute, resolving to a chronic inflammatory process, and to the ... Targeting pathogenic stromal cells has the potential to provide a cure for chronic inflammatory disorders such as rheumatoid ... In contrast to the paradigm that stromal cells are biochemically active but immunologically passive, there is now growing ... Stromal Cells, Synovial Membrane, Synovitis ... Stromal cells play a central role in the transformation of an ...

In the activation step, TIMP-2 bound to MT1-MMP on the cell surface acts as a receptor for proMMP-2. Subsequently, adjacent ... These findings elucidate a new function of the PEX domain: regulating MT1-MMP activity on the cell surface, which accelerates ... abolished the ability to activate proMMP-2 on the cell surface without affecting the proteolytic activities. In addition, ... expression of MT1PEX-F inhibited proMMP-2 activation and Matrigel invasion activity of invasive human fibrosarcoma HT1080 cells ...

... and demonstrates a unique mechanism by which an intracellular viral protein causes general suppression of natural killer cell ... Here we show that pp65, the main tegument protein of human cytomegalovirus, inhibited natural killer cell cytotoxicity by an ... Killer Cells, Natural, Kinetics, Membrane Glycoproteins, Mice, Natural Cytotoxicity Triggering Receptor 3, Phosphoproteins, ... Here we show that pp65, the main tegument protein of human cytomegalovirus, inhibited natural killer cell cytotoxicity by an ...

The researchers found that the membrane that protects and lubricates their joints appeared normal, in contrast to the inflamed ... Joint cell discovery offers new hope for people with rheumatoid arthritis Share Share Share Share ... We hope that knowing more about this cell type will also allow us to predict when patients are like to have a flare in their ... The study, published in Nature Medicine found that people with arthritis in long-term remission had a difference in cell ...

... of particular importance because they function in the immediate pericellular environment that modulates both cell-cell and cell ... and in the processing of active and latent-signaling molecules bound to the ECM or associated with the cell surface. Through ... and beta were expressed through at least the first 72 h of development and that this expression was targeted to the cell ... Amino Acid Sequence, Animals, Base Sequence, Cell Membrane, DNA, Complementary, Gene Expression Regulation, Developmental, Gene ...

... cells have an increased potential to generate B1a-like cells. Thus, Salmonella can have a prolonged influence on BM progenitor ... T cells, and some lineage-negative cells. After infection, the phenotype of BM progenitors rapidly but reversibly alters, with ... Most strikingly, the capacity of donor Sca-1(hi) cells to reconstitute an irradiated host is reduced; the longer donor mice are ... The generation of immune cells from BM precursors is a carefully regulated process. This is essential to limit the potential ...

Histology of the soft tissue lesion showed metastatic anaplastic meningioma with clumps of pleomorphic tumour cells which ... expressed epithelial membrane antigen, cytokeratin and P63 but were negative for other epithelial and mesenchymal markers. A ... Histology of the soft tissue lesion showed metastatic anaplastic meningioma with clumps of pleomorphic tumour cells which ... expressed epithelial membrane antigen, cytokeratin and P63 but were negative for other epithelial and mesenchymal markers. A ...

Five distinct membrane-type matrix metalloproteinases (MT-MMP) have been reported by cDNA cloning. However, the mt4-mmp gene ... Amino Acid Sequence, Animals, Blotting, Western, Breast Neoplasms, COS Cells, Carcinoma, DNA Primers, DNA, Complementary, ... Five distinct membrane-type matrix metalloproteinases (MT-MMP) have been reported by cDNA cloning. However, the mt4-mmp gene ... Human membrane type-4 matrix metalloproteinase (MT4-MMP) is encoded by a novel major transcript: isolation of complementary DNA ...

Priming of cells with IFNgamma did not have a synergistic effect on BAFF synthesis by FLS stimulated with bacterial products ... CONCLUSION: Our findings indicate that BAFF secretion by resident cells in target organs of autoimmunity is tightly regulated ... BAFF increases in RA target cells might be related to activation of the receptors of innate immunity. The purpose of this study ... Synovial Membrane, Toll-Like Receptor 2, Toll-Like Receptor 4, Toll-Like Receptor 9, Toll-Like Receptors, Tumor Necrosis Factor ...

Animals, Aorta, Apolipoproteins E, Biomarkers, Carotid Artery Diseases, Cells, Cultured, Cytokines, Disease Models, Animal, ... Female, Humans, Macrophages, Peritoneal, Male, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Monocytes, ...

11-beta-Hydroxysteroid Dehydrogenase Type 1, Arthritis, Cells, Cultured, Glucocorticoids, Humans, Intercellular Signaling ... Peptides and Proteins, Interleukin-1beta, Osteoarthritis, Signal Transduction, Spondylitis, Ankylosing, Synovial Membrane, ...

... cells and CD56(dim)CD16(+) cells. We tested the hypothesis that CD56(bright) NK cells can differentiate into CD56(dim) cells by ... Differentiated CD56(dim) cells had reduced IFN-gamma production but increased perforin expression and cytolysis of cell line ... Our results show that CD56(bright) cells can differentiate into CD56(dim) both in vitro, in the presence of synovial ... Our data support a linear differentiation model of human NK development in which immature CD56(bright) NK cells can ...

Analysis of the resulting alternatively patterned synapses revealed a causal relation between the radial position of T cell ... We used supported bilayer membranes and nanometer-scale structures fabricated onto the underlying substrate to impose geometric ... The immunological synapse is a specialized cell-cell junction that is defined by large-scale spatial patterns of receptors and ... The immunological synapse is a specialized cell-cell junction that is defined by large-scale spatial patterns of receptors and ...

11-beta-Hydroxysteroid Dehydrogenase Type 1, Arthritis, Cells, Cultured, Glucocorticoids, Humans, Intercellular Signaling ... Peptides and Proteins, Interleukin-1beta, Osteoarthritis, Signal Transduction, Spondylitis, Ankylosing, Synovial Membrane, ...

Acetylcysteine, Analysis of Variance, Animals, Antibodies, Monoclonal, Calcium-Binding Proteins, Cell Count, Choline O- ... Membrane Proteins, Motor Activity, Muscle Proteins, Nerve Degeneration, Parkinson Disease, Pedunculopontine Tegmental Nucleus, ...

... membranes, Permanox® slides, and glass slides, using nucleic acid staining and end-point confocal laser scanning microscopy. ... Using a microfluidic flow cell, we have investigated the relationship between both wall shear stress (τw ) and surface ... Using a microfluidic flow cell, we have investigated the relationship between both wall shear stress (τw ) and surface ... membranes, Permanox® slides, and glass slides, using nucleic acid staining and end-point confocal laser scanning microscopy. ...

... is a mitochondrial membrane protein, of as yet uncertain function. Its purported high expression on activated macrophages, has ... Arthritis, Rheumatoid, Cell Line, Down-Regulation, Humans, Inflammation, Interferon-gamma, Interleukin-4, Lipopolysaccharides, ... The translocator protein (TSPO) is a mitochondrial membrane protein, of as yet uncertain function. Its purported high ...

A modified immunohistochemical method detecting cytokine-producing rather than cytokine-binding cells was applied to determine ... A modified immunohistochemical method detecting cytokine-producing rather than cytokine-binding cells was applied to determine ... Synovial Membrane, Tumor Necrosis Factor-alpha ...

... encoding the inner nuclear membrane protein MAN1, which modulates TGFβ/bone morphogenetic protein signalling. Recent studies of ... encoding the inner nuclear membrane protein MAN1, which modulates TGFβ/bone morphogenetic protein signalling. Recent studies of ... Ancestrally Inclusive Musculoskeletal Single-cell (AIMS) Network * Our sites * Our team * Research activity ...

... encoding the inner nuclear membrane protein MAN1, which modulates TGFβ/bone morphogenetic protein signalling. Recent studies of ... encoding the inner nuclear membrane protein MAN1, which modulates TGFβ/bone morphogenetic protein signalling. Recent studies of ... Ancestrally Inclusive Musculoskeletal Single-cell (AIMS) Network * Our sites * Our team * Research activity ...

Synovial Membrane ... Ancestrally Inclusive Musculoskeletal Single-cell (AIMS) ...

Plasma Membrane Calcium-Transporting ATPases, Pregnancy, RNA, Messenger, Time Factors, Uterus ... Ancestrally Inclusive Musculoskeletal Single-cell (AIMS) Network * Our sites * Our team * Research activity ...