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*  Sarcoma-Producing Cell Lines Derived from Clones Transformed in vitro by Benzo[a]pyrene | Science
Transformed clones were obtained only with the carcinogen. Some of the transformed clones were responsible for cell lines that ... Sarcoma-Producing Cell Lines Derived from Clones Transformed in vitro by Benzo[a]pyrene ... Sarcoma-Producing Cell Lines Derived from Clones Transformed in vitro by Benzo[a]pyrene ... Sarcoma-Producing Cell Lines Derived from Clones Transformed in vitro by Benzo[a]pyrene ...
http://science.sciencemag.org/content/165/3896/917
*  Gene Targeting by Homologous Recombination in Drosophila | Science
This result has precedence in the examination of stably transformedDrosophila cell lines. Cherbas and Cherbas (35) observed ... Events that occur in mitotic cells of the germ line can be replicated as cells pass through S phase and may produce multiple y ... X-rays generate ds breaks in DNA and induce both homologous and nonhomologous recombination in somatic and germ line cells. DNA ... Female germ line targeting used two or three females by four males per vial; male germ line targeting used two or three males ...
http://science.sciencemag.org/content/288/5473/2013?ijkey=c0b7f89a4fc01f4a2d0678734509edb2439d4256&keytype2=tf_ipsecsha
*  Positive Control of Transformed Phenotype in Hybrids between SV40-Transformed and Normal Human Cells | Science
Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. Positive Control ... Positive Control of Transformed Phenotype in Hybrids between SV40-Transformed and Normal Human Cells ... Positive Control of Transformed Phenotype in Hybrids between SV40-Transformed and Normal Human Cells ... Positive Control of Transformed Phenotype in Hybrids between SV40-Transformed and Normal Human Cells ...
https://science.sciencemag.org/content/184/4143/1288/tab-e-letters
*  A new member of the leucine zipper class of proteins that binds to the HLA DR alpha promoter | Science
Several mutants derived from transformed human B cell lines are defective in expressing major histocompatibility complex (MHC) ... The failure to express a class II gene in at least one such mutant line has been mapped to the MHC class II X box, a conserved ... Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. A new member of ... These studies suggest that the hXBP-1 protein acts as a transcription factor in B cells. ...
http://science.sciencemag.org/content/247/4950/1581
*  Common Regulatory Variation Impacts Gene Expression in a Cell Type-Dependent Manner | Science
... despite the long separation time between tests of these cell lines, which demonstrated the stability of transformed B cells. ... Epstein-Barr virus-immortalized B cells (lymphoblastoid cell lines or LCLs), and T cells. Umbilical cord was chosen because it ... Fine-scale overlap of regulatory signals in three cell types. (A) Cell type-shared and cell type-specific independent cis eQTLs ... cell type-interaction term (the part of the equation that tests SNP's effects dependent on the cell type), where cell type- ...
https://science.sciencemag.org/content/325/5945/1246?ijkey=ad07a77ec946479de06fc9f1486fda65288f1344&keytype2=tf_ipsecsha
*  The human transcriptome across tissues and individuals | Science
... and two cell lines: Epstein-Barr virus-transformed lymphocytes (LCL) and cultured fibroblasts from skin [table S1 and (7)]. ... Transcriptome analysis of human tissues and cell lines reveals one dominant transcript per gene. Genome Biol. 14, R70 (2013). ... A role for the putative tumor suppressor Bin1 in muscle cell differentiation. Mol. Cell. Biol. 18, 566-575 (1998). pmid:9418903 ... the expression of which shows enrichment in the nucleus in female cell lines from ENCODE (17) and hence could be candidates to ...
http://science.sciencemag.org/content/348/6235/660?ijkey=0db646f5636f0eae04fff9a72be4a1cf063cea5c&keytype2=tf_ipsecsha
*  Cloning Genes Encoding MHC Class II-Restricted Antigens: Mutated CDC27 as a Tumor Antigen | Science
We genetically engineered HEK 293 cells, a transformed human kidney embryonic cell line, to express DRα, DRβ, DMA, DMB, and Ii ... other EBV-B cell lines and allogeneic melanoma cell lines, nor HEK 293-derived cell lines. Both 1088mel and 1102mel share DRβ1* ... T cells but was expressed at a lower level in the 1359mel line than in other cell lines tested. (B) 293IMDR4 cells were ... B cells (1359EBV), nor other MHC class II-positive EBV-B and tumor cell lines (Fig. 1A). CD4+ TIL1359 did not recognize cell ...
https://science.sciencemag.org/content/284/5418/1351?ijkey=c867e96e197b343e41dc5ecca5c26be32580c8e8&keytype2=tf_ipsecsha
*  Quantitation of HIV-1-Specific Cytotoxic T Lymphocytes and Plasma Load of Viral RNA | Science
... transformed B cell lines were incubated at 37°C for 1 hour in the presence of Na2 51CrO4 (Amersham) at 2 μCi/μl. The cells were ... Target cells (T2) prepulsed with 1 μM epitope peptide were plated at 104 cells per well. Effector cells were added directly ... The tetramer-positive cells make up 0.2 to 2.7% of all CD8+ cells (values are indicated in each plot) and are all CD38+. (D) ... 2B). The CD4 counts ranged from 235 to 711 cells/μl (median, 457 cells/μl), and viral load from ,500 to 359,500 copies per ...
https://science.sciencemag.org/content/279/5359/2103?ijkey=190a742ca7ed7ee8f0c77084781b21e7f606d408&keytype2=tf_ipsecsha
*  Senescence of nickel-transformed cells by an X chromosome: possible epigenetic control | Science
... carried in a mouse A9 donor cell line) to a nickel-transformed Chinese hamster cell line with an Xq chromosome deletion ... Senescence of nickel-transformed cells by an X chromosome: possible epigenetic control ... Senescence of nickel-transformed cells by an X chromosome: possible epigenetic control ... Senescence of nickel-transformed cells by an X chromosome: possible epigenetic control ...
http://science.sciencemag.org/content/251/4995/796
*  Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay | Science
Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. Normal cells of ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ...
http://science.sciencemag.org/content/222/4620/173
*  Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells | Science
... transformed lines of T cells (HTLV-II CM) contained large amounts of this growth activity and also supported the temporary ... Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells ... Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells ... Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells ...
http://science.sciencemag.org/content/242/4877/426
*  Telomerase Catalytic Subunit Homologs from Fission Yeast and Human | Science
... an adenovirus E1-transformed human embryonic kidney cell line (19). This cDNA clone (pGRN121) had a 182-bp insert relative to ... Expression of hTRT in telomerase-negative mortal cell strains (lanes 1 to 6) and telomerase-positive immortal cell lines (lanes ... A lambda cDNA library from the human 293 cell line, which has high levels of telomerase activity, was partitioned into 25 pools ... Polyadenylated RNAs from human testis and from the 293 cell line were amplified using a nested PCR strategy. The first primer ...
https://science.sciencemag.org/content/277/5328/955?ijkey=bc24e2e93579d688693caf6cf3feb015b1fd027e&keytype2=tf_ipsecsha
*  Addiction to Oncogenes--the Achilles Heal of Cancer | Science
... the revertant esophageal cancer cell line still expressed moderately high levels of cyclin D1. Thus, the parental cells were " ... This appears to be the case in myc-transformed (13) or SV40-transformed (14) fibroblasts. The need for continued activity of a ... 5. Cell proliferation and cell fate determination. Cancer Medicine has a chapter on cell proliferation, differentiation, and ... Growth of a human pancreatic cancer cell line carrying a mutant K-ras oncogene, but not one carrying normal K-ras [HN15], was ...
https://science.sciencemag.org/content/297/5578/63?ijkey=54b02fece24992f4515ccd44d80d96882ac2e7c2&keytype2=tf_ipsecsha
*  New Products | Science
DNA Repair knockdown cell Lines A range of highly efficient knockdown cell lines covering the base excision DNA repair pathway ... The newly launched Eppendorf Eporator offers a fast, simple, and safe way to transform bacteria, yeast, and other ... The DNA repair knockdown cell lines provide essential tools to address these needs. All DNA repair knockdown cell line products ... The knockdown efficiency of the new DNA repair knockdown cell lines on target genes, as measured by RT-PCR, is 63 to 98%. ...
http://science.sciencemag.org/content/333/6039/243.1
*  A promoter that drives transgene expression in cerebellar Purkinje and retinal bipolar neurons | Science
Three independent transgenic lines, germ line transformed with an L7-beta-galactosidase fusion gene, exhibit beta-galactosidase ... A genomic clone encoding the Purkinje cell-specific L7 protein has been isolated and utilized to drive the expression of beta- ... Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. A promoter that ... The transgenic murine lines can be used to obtain populations of marked Purkinje and bipolar neurons. Similar L7 promoter ...
http://science.sciencemag.org/content/248/4952/223
*  Patients with familial adenomatous polyposis harbor colonic biofilms containing tumorigenic bacteria | Science
For each cell subset and total number of IL-17-producing cells (gray dotted line box), the overall P value is shown and was ... across IL-17-producing cell types was calculated by using two-way analysis of variance testing based on log-transformed data ( ... Dotted lines delineate the luminal edge of the colonic epithelial cells. Images are representative of n = 4 to 23 tissue ... Two-group cell subset and total number of IL-17-producing cell comparisons were analyzed by Mann-Whitney U test and are ...
http://science.sciencemag.org/content/359/6375/592?ijkey=efed6440313da271d42b7e6d83343600d67fef69&keytype2=tf_ipsecsha
*  Cytokinin Activation of Arabidopsis Cell Division Through a D-Type Cyclin | Science
3A). Such plants were crossed to Arabidopsis lines carrying a heat-shock promoter (hsp)-driven FLP gene (20), so that FLP- ... we used the yeast site-specific recombinase FLP to activate CycD3 expression in the progeny of transformedArabidopsis plants. ... Cells were rendered quiescent by incubation of day 7 cells in sucrose-free medium (with hormones) for 24 hours, followed by ... CycD3 induction could be a direct response to cytokinin or an indirect response caused by cells reaching a certain cell cycle ...
https://science.sciencemag.org/content/283/5407/1541?ijkey=6041d5c7f8b3cbba8218df0067f092fafa3bd86e&keytype2=tf_ipsecsha
*  Site-specific increased phosphorylation of pp60v-src after treatment of RSV-transformed cells with a tumor promoter | Science
Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. Site-specific ... Site-specific increased phosphorylation of pp60v-src after treatment of RSV-transformed cells with a tumor promoter ... Site-specific increased phosphorylation of pp60v-src after treatment of RSV-transformed cells with a tumor promoter ... Site-specific increased phosphorylation of pp60v-src after treatment of RSV-transformed cells with a tumor promoter ...
https://science.sciencemag.org/content/229/4720/1393/tab-e-letters
*  Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells | Science
Southern blots of genomic DNA's from A431 and other human cell lines were probed with either a v-erb B gene fragment or a human ... may result in the overproduction of EGF receptor protein or the appearance of the transformed phenotype (or both). ... Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. Amplification ... Furthermore, A431 cells contain a characteristic, prominent 2.9-kilobase RNA. These results are consistent with the hypothesis ...
http://science.sciencemag.org/content/224/4647/417
*  Extension of Life-Span by Introduction of Telomerase into Normal Human Cells | Science
Cloned normal diploid cells could replace established tumor cell lines in studies of biochemical and physiological aspects of ... We have not observed any gross phenotypic or morphological characteristics of transformed cells (such as loss of contact ... This range of telomerase activity is similar to that observed for tumor cell lines (13). Thirty-three RPE clones and 24 BJ ... The gray horizontal bar represents the mean TRF of the cell population at the time of transfection. The dashed horizontal lines ...
http://science.sciencemag.org/content/279/5349/349?ijkey=1a5251771beec7c20289f94ce631a6c26a1f92c0&keytype2=tf_ipsecsha
*  Rovibrational quantum state resolution of the C60 fullerene | Science
The individual line positions are listed in (39). (B) The residuals from the fit of Eq. 6 to these line positions, summarized ... These hot molecules flow into a cell anchored to a cryogenic cold finger, where they are thermalized close to the cell-wall ... Fourier transform spectroscopy with a laser frequency comb. Nat. Photonics 3, 99-102 (2009). doi:10.1038/nphoton.2008.293. ... blue dashed line) and average vibrational energy (red solid line) increase strongly as a function of temperature. About 6 to 8 ...
http://science.sciencemag.org/content/363/6422/49
*  NextGen Results | Science
There, I explained to him that white blood cells express germ-line encoded receptors that specifically sense different ... Ideally, transforming the goals and designs implemented from the late 1700s until the mid-1800s would promote a more ... I'd explain how her cells, HeLa cells, helped improve human life by facilitating the development of the polio vaccine, anti- ... him to take an even stronger line in the (then future) Edict of Milan on the lines of not merely tolerance of different ...
http://science.sciencemag.org/content/suppl/2013/07/02/341.6141.28.DC1
*  A shed NKG2D ligand that promotes natural killer cell activation and tumor rejection | Science
Immune cells, including natural killer (NK) cells, recognize transformed cells and eliminate them in a process termed ... Surprisingly, both cell lines were rejected by syngeneic B6 mice compared with cells transduced with empty vector (Fig. 1B and ... cells (Fig. 1C and fig. S5). Hence, NK cells but not CD8+ cells participate in the rejection of B16-secMULT1. B16 cells with ... cells and some T cells use activating receptors such as NKG2D to recognize and eliminate infected and transformed cells that up ...
https://science.sciencemag.org/content/348/6230/136?ijkey=55a38661102822a44f41d4019a09057a634051c2&keytype2=tf_ipsecsha
*  The Microbial Metabolites, Short-Chain Fatty Acids, Regulate Colonic Treg Cell Homeostasis | Science
Horizontal lines show the mean; error bars denote the SD. In (B) and (C), each symbol or bar represents pooled cTregs from ... 1, B and C). In vitro treatment increased IL-10 production but not that of transforming growth factor-β (TGFβ), a Treg-mediated ... B) Cell numbers for the data in the top panel of (A). (C) Cell numbers for the data in the bottom panel of (A). Symbols in (B) ... are injected with either naïve T cells, which results in severe colitis, or naïve T cells in combination with Tregs, which ...
https://science.sciencemag.org/content/341/6145/569?ijkey=a5d99380197e823688bc2ed969601317b1f5d1f1&keytype2=tf_ipsecsha
*  Insertion of a new gene of viral origin into bone marrow cells of mice | Science
HSVtk DNA was also used to transform mouse bone marrow cells in vitro. Transformed marrow cells injected into irradiated and ... Enter multiple addresses on separate lines or separate them with commas. You are going to email the following. Insertion of a ... DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk+ mouse L cells to a ... Insertion of a new gene of viral origin into bone marrow cells of mice ...
http://science.sciencemag.org/content/208/4447/1033