Metabolic targeting of hypoxia and HIF1 in solid tumors can enhance cytotoxic chemotherapy | PNAS
MEFs were transformed by stable transfection with an activated H-ras expression plasmid. The RKOShHIF1α cell line has been ... Cell Lines and Tumor Xenografts.. RKO and Su.86 cells were obtained from the American Type Culture Collection (ATCC, Manassas, ... 1 b) and MEF cell lines (SI Fig. 6b ). These genetically matched cells show that echinomycin treatment can block the adaptive ... Louis, MO). Tumor xenografts were established by injecting 5 × 106 RKO cells, 1 × 107 Su.86 cells, or 2 × 106 Hras-MEF cells s. ...https://www.pnas.org/content/104/22/9445
Inhibition of glycolipid shedding rescues recognition of a CD1+ T cell lymphoma by natural killer T (NKT) cells | PNAS
One hallmark trait of transformed cell lines is altered glycolipid expression and shedding. Glycosphingolipids (GSLs) are ... Cell Lines.. The murine L5178Y-R (T cell lymphoma) and YAC-1 (T cell leukemia) cell lines were cultured in DMEM and RPMI medium ... murine tumor cell lines, we show that shed glycolipids from one tumor line, the murine L5178Y-R T cell lymphoma cell line, can ... B) Murine CD1+ hematopoietic tumor cells are not recognized by NKT cells. The Vα14+ NKT cell hybridoma cell line, DN32.D3, was ...https://www.pnas.org/content/99/12/8197?ijkey=b8e598201fcbe3a14844bf5a3ba4d0ee820dfb94&keytype2=tf_ipsecsha
Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with...
... we examined the expression of the transfected genes in a series of transformed BRK cell lines. In cell lines derived by ... A-1 is a cell line established by transfection with the E1A-expressing plasmid alone. A/1/2-1 to A/1/2-10 are cell lines ... A/B-1 and A/B-2 are cell lines transformed with E1A and E1B-19-kDa. HeLa, 293, and HP1.14 (a derivative HeLa cells expressing ... HCMV IE1 and IE2 genes and their products are not present in transformed BRK cell lines. (A) Lack of expression of the IE1 or ...http://www.pnas.org/content/94/7/3341.full
ACTH protects against glucocorticoid-induced osteonecrosis of bone | PNAS
This transformed cell line retains large numbers of division-competent uncommitted precursor cells, even in the presence of ... 2Di). Adding to our confidence was the observation that the transformed human osteoblast-like cell line MG63 displayed largely ... response of the transformed osteoblast-like cell line MG63 to ACTH was qualitatively similar to that of nontransformed cells ( ... Human mesenchymal cell cultures or transformed MG63 cells were similarly incubated for 28 days, as described (12). ...https://www.pnas.org/content/107/19/8782
Homozygous deletion of glycogen synthase kinase 3β bypasses senescence allowing Ras transformation of primary murine...
In contrast to primary cells, immortalized rodent cell lines are readily transformed by RasV12 alone. To further explore the ... Cells.. MEF cell lines isolated from GSK3β−/− mouse embryo and wild-type littermate were established in culture as immortal ... Immortalized rodent cells can readily be transformed by activated Ras or other oncogenes, leading to a transformed phenotype in ... Cell Proliferation Assay.. Cell numbers were assessed by staining with crystal violet as an indicator of DNA content. Cells ...https://www.pnas.org/content/105/13/5248
Table of Contents - November 07, 2017, 114 (45) | PNAS
KO of 5-InsP7 kinase activity transforms the HCT116 colon cancer cell line into a hypermetabolic, growth-inhibited phenotype ... Correction for Tong et al., IgH isotype-specific B cell receptor expression influences B cell fate PNAS November 7, 2017 114 ( ... Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling Youichi Uda, Yuhei Goto, ... Cell Biology. *. You have access. Large G protein α-subunit XLαs limits clathrin-mediated endocytosis and regulates tissue iron ...http://www.pnas.org/content/114/45/
Detection of functional single-nucleotide polymorphisms that affect apoptosis | PNAS
Response of EBV-Transformed B Cells to γ Radiation (γIR). A total of 120 EBV-transformed lymphoblast cell lines (LCLs) derived ... Each cell line was measured at least three times. The identification, average apoptosis, and standard error for each cell line ... The total number of cell lines in our analysis was reduced to 113 after elimination of data from cell lines that never reached ... the experiment was repeated at least three times for each cell line. The data for each cell line was quite reproducible, with ...https://www.pnas.org/content/102/45/16297
An endotoxin-induced serum factor that causes necrosis of tumors | PNAS
TNF is toxic in vitro for two neoplastic cell lines; it is not toxic for mouse embryo cultures. We propose that TNF mediates ... and that it may be responsible for the suppression of transformed cells by activated macrophages. ... A variety of tests indicate that TNF is not residual endotoxin, but a factor released from host cells, probably macrophages, by ...https://www.pnas.org/content/72/9/3666?ijkey=e83ab9fb5c652332ab95d4d7933b03597d76aa9e&keytype2=tf_ipsecsha
The HLA-DQ2 gene dose effect in celiac disease is directly related to the magnitude and breadth of gluten-specific T cell...
... van den Elsen for providing the HLA-class II negative EBV-transformed B cell line, G. Nolan for ϕ-NX-A cells, C. Mazzilli for ... Upper) Absence of HLA-DQ2 on an HLA class II negative B cell line (thin line), and presence of HLA-DQ2 on the cell line ... and β-chains into HLA class II-negative cells (EBV-transformed lymphoblastoid cell line SJO) was performed as described (16). ... bound strongly to HLA-DQ isolated from both HLA-DQ2-homozygous cell lines and the HLA-DQ2.5/DQ2.2-heterozygous cell lines (Fig ...http://www.pnas.org/content/100/21/12390?ijkey=5b935b2102d925c44d4f10f8f7428349f829ece7&keytype2=tf_ipsecsha
A highly conserved kinase is an essential component for stress tolerance in yeast and plant cells | PNAS
Total RNAs were extracted from independent lines transformed with pBIN-35S-At-DBF2 (1) and (2), pBIN-35S-CaMVter (3), and pBIN- ... Tobacco Cell Transformation and Recombinant T-DNA Vector Construction.. BY2 cells were stably transformed as described (5) by ... After 2 days of growth, cell density was adjusted to 1.5 × 106 cells ⋅ ml−1 and 1 ml cells was dropped on solid medium and ... BY2 cells were transformed by A. tumefaciens with recombinant T-DNA vectors containing At-DBF2 driven by CaMV 35S RNA promoter ...https://www.pnas.org/content/96/10/5873?ijkey=acbf1cc0fda4a2d4e4e9d79f90b539368660e872&keytype2=tf_ipsecsha
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis | PNAS
... using nine combinations of one of three overexpressing lines (Cy5 label) and one of three control lines transformed with an ... 4 B shows the GSL contents of the Myb28-overexpressing cell suspension cultures. Nontransformed and empty-vector-transformed ... Arabidopsis T87 cultured suspension cells (41) were transformed with the fusion construct of CaMV35S promoter linked to Myb28 ... Log ratio (base 2) of signal intensity in Myb28-overexpressing T87 cell suspension cultures (T87OX) to control lines (empty- ...http://www.pnas.org/content/104/15/6478.full
Ectopic expression of retinoic acid early inducible-1 gene (RAE-1) permits natural killer cell-mediated rejection of a MHC...
... and H60 were identified as ligands for mouse NKG2D by expression cloning these cDNA from a mouse transformed lung cell line (22 ... Cell Lines and Transfectants.. The RMA (H-2b positive) lymphoma cell line and the mutant RMA-S (H-2b deficient) cell line ( ... cells rejected an MHC class I-deficient tumor cell line (RMA-S) but they did not reject the same cell line if it expressed MHC ... virus-infected cells, and possibly "stressed" or transformed cells. In contrast to adaptive immunity, innate immune cells ...https://www.pnas.org/content/98/20/11521?ijkey=cc5fc21d4cd71c5a3fb0d7327bd58da06cc98f02&keytype2=tf_ipsecsha
Epstein-Barr Virus Nuclear Antigen 3C binds to BATF/IRF4 or SPI1/IRF4 composite sites and recruits Sin3A to repress CDKN2A |...
... transforms primary B cells into lymphoblastoid cell lines. PLoS Pathog 9(2):e1003187. ... Cell Lines.. LCLs transformed by a recombinant EBV BACmid in which EBNA3C was C-terminally tagged with HA and Flag epitopes ( ... results in continuously proliferating lymphoblastoid cell lines (LCLs). LCLs and EBV-infected cells in T-cell immune-deficient ... 2011) Epstein-Barr virus nuclear antigen 3C regulated genes in lymphoblastoid cell lines. Proc Natl Acad Sci USA 108(1):337-342 ...https://www.pnas.org/content/111/1/421
Epstein-Barr virus nuclear antigen 3A partially coincides with EBNA3C genome-wide and is tethered to DNA through BATF complexes...
... transforms primary B cells into lymphoblastoid cell lines. PLoS Pathog 9(2):e1003187. ... Cell Lines.. A recombinant EBV in which EBNA3A is C-terminally tagged with FLAG and HA epitope (EBNA3AFHA) was used to generate ... 2006) EBNA-3B- and EBNA-3C-regulated cellular genes in Epstein-Barr virus-immortalized lymphoblastoid cell lines. J Virol 80(20 ... 2003) Epstein-Barr Virus nuclear protein EBNA3A is critical for maintaining lymphoblastoid cell line growth. J Virol 77(19): ...https://www.pnas.org/content/112/2/554
The cell surface leucine-rich repeat receptor for AtPep1, an endogenous peptide elicitor in Arabidopsis, is functional in...
Expression of the PEPR1 gene was confirmed by RT-PCR analysis of RNA from three independently transformed tobacco cell lines ( ... 014538 line; lane 3, SALK_059281 line; lane 4, SALK_064539 line, which is a control line unrelated to At1g73080 in which the ... Lower) RT-PCR analyses of At1g73080 mRNA in wild-type cells and in three transgenic cell lines (#2, #3, and #4). Expression of ... Each set of cells was dispersed in 300 ml of extraction buffer, the cells were disrupted in a Parr Cell Disruption Bomb (Parr ...https://www.pnas.org/content/103/26/10104
Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth | PNAS
Cell Lines and Transfections. MDA-MB-435 cells were grown in DMEM supplemented with 10% FBS. For transfections, 6-cm plates ... This balance is disrupted when mammary epithelial cells become transformed: ephrin-B2 expression in the epithelial cells is ... Repulsive EphB4 signaling could favor the dissemination of metastatic cells by decreasing cell-cell and cell-matrix adhesion (4 ... EphB4ΔC-EGFP tumor cells attract more HUVE cells (red) than EGFP-F cells. (Scale bar represents 50 μM.) HUVE cells that had ...https://www.pnas.org/content/101/15/5583?ijkey=f09428779536ac91717c1dab54d161a17ef914f0&keytype2=tf_ipsecsha
Constitutive activation of a slowly migrating isoform of Stat3 in mycosis fungoides: Tyrphostin AG490 inhibits Stat3 activation...
... or v-Src-transformed cell lines (10, 11). Our MF cells are HTLV-1-negative and express very low levels of the Src-family kinase ... and karyotyping verified the tumor cell origin of the cell line (2, 3). Because the tumor cell line during growth is ... The My-la 2039 cell line was studied between cell population doublings 10 and 100, whereas the My-la 2059 cell line has more ... Three MF cell lines and a nonmalignant T cell line (PLT) were IL-2-starved before stimulation with IL-2 (500 units/ml) for 10 ...https://www.pnas.org/content/94/13/6764?ijkey=eeceefbb38ec8964b2998e517eca245ac941d2d7&keytype2=tf_ipsecsha
SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage | PNAS
2RA, which is an simian virus 40 T-antigen transformed derivative of the human lung fibroblast WI 38 cell line, was obtained ... 6A, both purified hTOP1 and hTOP1 in a nuclear extract prepared from human 2RA cells (T-antigen-transformed WI38 cells) were ... C) Yeast cell lysates from JN362a cells harboring HF-Smt3p (lanes 1 and 2) or cells harboring HF-Smt3p(G97) (lanes 3 and 4) ... CPT induces SUMO-1 conjugation to hTOP1 in HeLa cells. HeLa cells were treated with 10 μM CPT (+) or DMSO (−) for 15 min. Cells ...http://www.pnas.org/content/97/8/4046
Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: essential role for...
In a range of cell lines, immobilized geldanamycin bound elements of a major class of heat shock protein (HSP90) in a stable ... As an approach to the study of v-src-mediated transformation, we examined ansamycin action in transformed cells and found that ... Cell grafts for epilepsy treatment. Human induced pluripotent stem cell-derived medial ganglionic eminence cells alleviate ... Glial Cell Line-derived Neurotrophic Factor Reverses Ethanol-mediated Increases in Tyrosine Hydroxylase Immunoreactivity via ...https://www.pnas.org/content/91/18/8324?ijkey=a0183d77a9e5a0e314bd94e96039ee63464a717c&keytype2=tf_ipsecsha
Reactive oxygen generated by Nox1 triggers the angiogenic switch | PNAS
Cell Lines.. NEF2 is a vector-control line developed from NIH 3T3 cells. YA28 and YA26 NIH 3T3 lines stably express Nox1 and ... The Nox1 gene transforms NIH 3T3 cells, rendering them capable of forming well vascularized tumors, whereas the parent cells ... Lane 1, NEF2 (vector-control) cells; lane 2, Nox1-expressing YA28 cells; lane 3, YA28 cells coexpressing catalase (ZC-5 cells; ... Cell Proliferation.. Cells were grown in 12 wells of a 96-well plate by adding 1.75 × 103 cells in media with 10% (vol/vol) FBS ...http://www.pnas.org/content/99/2/715
Direct visualization of antigen-specific T cells: HTLV-1 Tax11-19- specific CD8+ T cells are activated in peripheral blood and...
Histocompatibility typing was performed on PBLs or Epstein-Barr virus-transformed lymphoblastoid cell lines by the Tissue ... Epstein-Barr virus-transformed B cells (our unpublished data). Moreover, we were able to detect Tax-specific T cells with Tax- ... cells are not uniformly activated. The cytokine-negative cells may represent a dormant cell population not actively ... Gag77-85-loaded HLA-A2/IgG was stably bound on the cell surface and detected using PE-labeled goat anti-mouse Ig (heavy line). ...https://www.pnas.org/content/95/13/7568?ijkey=c26d509074af6e9ed5939b36d414f5a80ac0af61&keytype2=tf_ipsecsha
Dendritic cell fate is determined by BCL11A | PNAS
... bore a striking resemblance to its binding distribution in the human EBV-transformed lymphoblastoid cell line (LCL) GM12878, ... Inducible shRNA Knockdown and RT-PCR in the CAL-1 pDC Cell Line.. CAL-1 cells were stably transduced with a retrovirus ... Retroviral Transduction of B-Cell Lines and LymphoChip Gene Expression Profiling.. Phoenix-A (ΦNX-A) amphotropic 293 cells were ... Complementary experiments using human cell lines expand these observations made in mice and imply determination of pDC cell ...http://www.pnas.org/content/111/11/E998.long
Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity | PNAS
... which consisted of the six cell lines in Table S1 and two RB MSC lines. Overall immunosuppression response (transformed ... Table S1 for descriptions of all cell lines used). MSCs from all cell lines suppressed activation of CD4+ and CD8+ T cells in a ... Additional cell lines used for validation are described in Tables S2 and S3. Bone marrow-derived cell lines 110877, 1F3422, ... 16 different cell line samples from one experiment) with linear fit (solid line) and reference AUCpred = AUCexp line for ...https://www.pnas.org/content/114/13/E2598
Robust hepatitis C virus infection in vitro | PNAS
... and nonhepatic cell lines (HeLa, HEK293, HL-60, U-937, and EBV-transformed B cells). Besides the Huh-7.5.1 cells, only the Huh- ... The human hepatocarcinoma cell line HepG2 (American Type Culture Collection) (29) and Epstein-Barr virus-transformed B cells ... Our initial efforts focused on the use of Huh7.5.1 cells, a cell line derived from Huh7.5 cells that has been shown to be ... Huh-7.5.1 cells were derived from the Huh-7.5 GFP-HCV replicon cell line I/5A-GFP-6 (30), kindly provided by Charles Rice ( ...https://www.pnas.org/content/102/26/9294.full
A simplified system for generating recombinant adenoviruses | PNAS
In the human colorectal cancer cell line HCT116, efu was ≈20- to 200-fold lower than that achieved in 293 or 911 cells. ... coli BJ5183 cells was used to transform DH10B cells, a recA strain in which high-quality and high yields of plasmid DNA can be ... vi) The packaging cell lines (293, 911, or 911E4) are each highly transfectable by lipid-DNA complexes. The 293 and 911 cells ... Finally, the linearized recombinant plasmid is transfected into adenovirus packaging cell lines, e.g., 911 or 293 cells. ...https://www.pnas.org/content/95/5/2509?ijkey=8eefabe9bce8c66f915617ea1361e302204f85ee&keytype2=tf_ipsecsha