Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose,...
Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ... Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ... Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ... Perifusion of a Clonal Cell Line of Simian Virus 40-Transformed Beta Cells: Insulin Secretory Dynamics in Response to Glucose, ...http://diabetes.diabetesjournals.org/content/34/2/115
Generation and culture of Epstein-Barr virus-transformed lymphoblastoid cell lines and dermal fibroblasts are described in the ... 2C and D). Cell number at confluence was consistently slightly lower in the knockdown cells. Both cell lines subsequently ... Hormonal induction of differentiation in all cell lines resulted in characteristic morphological changes within 24 h, cell ... Differences in cell proliferation rates were assessed by fitting linear regression lines then testing for gradient equality. ...http://diabetes.diabetesjournals.org/content/60/3/925
Metformin Prevents High-Glucose-Induced Endothelial Cell Death Through a Mitochondrial Permeability Transition-Dependent...
... was also investigated because it inhibits PTP opening in transformed cell lines. Cyclosporin A (CsA), the reference PTP ... Cell culture conditions.. The immortalized human dermal microvascular endothelial cell line HMEC-1 (23) was a generous gift ... Because glucose cytotoxicity was prevented by PTP inhibitors in either an immortalized cell line (Fig. 6A) or primary cells ( ... Control cells (A), cells incubated overnight in the presence of 100 μmol/l metformin (C), or cells directly exposed to 1 μmol/l ...http://diabetes.diabetesjournals.org/content/54/7/2179
CCN5, a novel transcriptional repressor of the transforming growth factor β signaling pathway. Mol Cell Biol 2011;31:1459-1469 ... The effect of BMP4 on adipogenic commitment was first shown with the multipotent stem cell line established from mouse embryos ... Pancreatic β cell dedifferentiation as a mechanism of diabetic β cell failure. Cell 2012;150:1223-1234pmid:22980982. ... Promoting adipose cell recruitment in the subcutaneous adipose tissue rather than merely inflating the cells would be ...http://diabetes.diabetesjournals.org/content/62/9/2997
Circulating Preproinsulin Signal Peptide-Specific CD8 T Cells Restricted by the Susceptibility Molecule HLA-A24 Are Expanded at...
... for expression in mammalian cell lines. HLA-A*2402 was cloned from Epstein Barr virus-transformed B cells and inserted between ... Generation and characterization of surrogate β-cell line. The myelogenous leukemia cell line K562 is transfected with HLA-A* ... 109 cells from the relevant cell line were harvested and washed with PBS, and cell pellets were stored at −80°C. ... solid line) was slightly higher than in the K562-A24 cells (dashed line). Isotype control staining was similar in both cell ...http://diabetes.diabetesjournals.org/content/61/7/1752?frame=sidebar&with-ds=yes
... and its high expression is also seen in vascular smooth muscle cells as well as in breast cancer cell lines (47,48). Founder ... Log2-transformed expression signals were normalized separately per tissue as follows. Quantile normalization was performed ... The Multiple Tissue Human Expression Resource (MuTHER; www.muther.ac.uk) includes lymphoblastic cell lines and skin and adipose ... and lymphoblastic cell line gene expression data from the MuTHER Consortium, comprising healthy female twins of European ...http://diabetes.diabetesjournals.org/content/62/5/1746
Exposure of transformed macrophage cell lines and primary macrophages to Cu2+-oxidized LDL has been reported to lead to ... The cells were gently scraped from the surface of the plate using a cell scraper and were counted using a cell counter (Coulter ... The cell population was characterized using a Mac-2 antibody and consisted of ,98% Mac-2-positive cells. The cells then were ... These cells were centrifuged at 650g for 5 min, and the pellet was resuspended in fresh medium. The cells were plated on tissue ...http://diabetes.diabetesjournals.org/content/53/12/3217
The European Collection of Cell Cultures for providing the transformed cell lines, and all the patients who contributed to the ... Transformation of cell lines and tissue culture.. The cell lines MY0104 (R171X), ABO154 (I414G415ATCG→CCA), MYO414 (P291fsinsC ... The advantage of using lymphoblastoid cells over engineered cell lines, transient transfections, or transgenic mice is that the ... by comparison of the median ΔCt value of mutated cell lines with the median ΔCt value obtained from the control cell lines by ...http://diabetes.diabetesjournals.org/content/53/2/500
Hyperthermia With Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis | Diabetes
... stress or activation of JNK decreases the activity of Akt in SV-40-transformed hamster-derived β-cell line HIT-T15 cells, ... MES certainly prevents β-cell apoptosis and preserves β-cell mass in vivo; however, in vitro experiments using MIN6 cells ... A: MIN6 cells were treated with HS and/or MES for 10 min. At 10 h after the treatment, the cells were harvested, and the HSP72 ... MIN6 cells were treated with MES and/or HS for 10 min. At 10 h after the treatment, the cells were incubated with or without ...http://diabetes.diabetesjournals.org/content/61/4/838.long
Regulation of the Pancreatic Pro-Endocrine Gene Neurogenin3 | Diabetes
This difference could reflect differences between cells in vivo and transformed cell lines. Although the islet and ductal cell ... Surprisingly, the human ngn3 promoter drives transcription in all cell lines tested, including fibroblast cell lines. In ... or nuclear extracts of the indicated cell lines. The major complex binding to the site in both islet cell lines is ablated by ... the human promoter drives transcription at a high level in all of the tested cell lines, including the fibroblast cell lines. ...https://diabetes.diabetesjournals.org/content/50/5/928?ijkey=e212c00a4393351d93caaff984ddcbc4ad3df4ab&keytype2=tf_ipsecsha
Genomic DNA was obtained from cultured lymphoblastoid cell lines by proteinase K and phenol/chloroform extraction procedure ... For log-transformed variables, the results are the back-transformed least-square means ± SE derived from the 95% confidence ... A central component in glucose-induced insulin secretion is the ATP-sensitive potassium (KATP) channel of the β-cell. ... First, SUR1 has been described mainly in pancreatic β-cells (6). Second, INS levels, which are variably correlated with insulin ...http://diabetes.diabetesjournals.org/content/50/3/697
Islet Encapsulation: Physiological Possibilities and Limitations | Diabetes
A stem cell line, human embryonic stem cells or induced pluripotent stem cells, could be genetically modified by removal of all ... such as recurrence of autoimmunity and inadequate immune control of virally infected or transformed cells in the product. ... Such a stem cell line will likely escape natural killer cell-mediated killing, as it will be recognized as "self" because of ... Generation of transplantable insulin-producing cells from human embryonic stem cells or induced pluripotent stem cells is at ...https://diabetes.diabetesjournals.org/content/66/7/1748.long
Carnosine as a Protective Factor in Diabetic Nephropathy | Diabetes
Human SV 40-transformed mesangial cells were characterized as previously described (16). All cell lines were cultivated in ... Cell culture.. Human SV 40-transformed mesangial cells as well as human thermosensitive SV 40-transformed podocytes were used ... Stable cell lines of T-SV40 immortalized human glomerular mesangial cells. Kidney Int49 :267 -270,1996. ... The in vitro studies were performed by P.B., S.S., and B.A.Y., E.R., P.M., and M.A.S. provided the cell lines. C.F. provided ...http://diabetes.diabetesjournals.org/content/54/8/2320.full
Human β-Cell Proliferation and Intracellular Signaling Part 2: Still Driving in the Dark Without a Road Map | Diabetes
The gray lines are molecules and pathways that are known to exist in rodents but are unknown in human β-cells. The human β-cell ... However, and in contrast to BTC, two EGFR ligands, EGF or transforming growth factor (TGF)-α only modestly increase rodent β- ... While the majority of information described above is derived from rodent β-cells or cell lines, it is likely that many of the ... Leptin receptors have been reported to be expressed in primary murine β-cells and in insulinoma cell lines (70-72). The major ...http://diabetes.diabetesjournals.org/content/63/3/819
For the VEGF gene assay, RAGE overexpressing ECV304 cells (the HUVEC-derived cell line) were used. These cells were stimulated ... a rabbit transforming growth factor-β1 polyclonal antibody (dilution 1:50; Santa Cruz Biotechnology), a goat connective tissue ... C6 rat glioma cells were used for this assay according to a previously described procedure (25,27). Briefly, cells were ... Vascular cell adhesion molecule-1 and VEGF assays.. Vascular cell adhesion molecule (VCAM)-1 assay was performed by ELISA as ...http://diabetes.diabetesjournals.org/content/55/9/2510
156 lymphoblastoid cell lines, and 160 skin tissues derived from a subset of healthy female twins of the MuTHER resource (19). ... were natural log-transformed before analysis, and results of each study were combined by fixed-effect inverse variance weighted ... including pancreatic β-cells (47,48). Its dysfunction in β-cells may lead to islet inflammation and impaired β-cell function, ... RasGRP1 and RasGRP3 regulate B cell proliferation by facilitating B cell receptor-Ras signaling. J Immunol 2005;175:7179-7184 ...http://diabetes.diabetesjournals.org/content/62/1/291
Allele-Specific Transcriptional Activity at Type 2 Diabetes-Associated Single Nucleotide Polymorphisms in Regions of Pancreatic...
Cell culture.. Two insulinoma cell lines, rat-derived 832/13 (21) (C.B. Newgard, Duke University, Durham, NC) and mouse-derived ... Type 2 diabetes (T2D) associated polymorphisms regulate expression of adjacent transcripts in transformed lymphocytes, adipose ... 2A, B). Of these, rs1635852 showed differential allelic enhancer activity in both orientations in both cell lines. The risk ... Isolation of INS-1-derived cell lines with robust ATP-sensitive K+ channel-dependent and -independent glucose-stimulated ...http://diabetes.diabetesjournals.org/node/33006.full.print
In vitro cell viability assay.. Transformed NIT-1 and MIN-6 β-cells were seeded at 1 × 106 cells/well in 24-well plates and ... cell death to baseline levels in both β-cell lines. These data indicate that XIAP provides marked protection of β-cells from ... In vitro cytokine-induced β-cell death was decreased to baseline levels in XIAP-transduced MIN-6 and NIT-1 cell lines compared ... Ad-XIAP protects MIN-6 and NIT-1 β-cells from cytokine-induced death (B and C). β-Cells were transduced with 0 or 5 pfu/cell Ad ...http://diabetes.diabetesjournals.org/content/54/9/2533
VEGF protein in these specimens was found in cytoplasm of endothelial cells lining small vessels and in cardiomyocytes (Fig. 2 ... transforming growth factor (TGF)-β, and angiopoietin-1. NO stimulates endothelial proliferation and migration functions as an ... Specifically, immunoreactivity was seen in the cytoplasm of cardiomyocytes and endothelial cells lining the small vessels (Fig ... and 90th percentiles as lines on a bar centered around the mean and the 5th and 95th percentiles as error bars; the mean line ...http://diabetes.diabetesjournals.org/content/53/9/2383
Naturally Arising Human CD4 T-Cells That Recognize Islet Autoantigens and Secrete Interleukin-10 Regulate Proinflammatory T...
A: The HLA-DRB1*0401 Epstein-Barr virus B-cell line Preiss was labeled with CFSE and pulsed with either IA-2 709-736 (filled ... To study the role of cytokines in suppression, anti-IL-10Rα (clone 37607.11), anti-transforming growth factor-β1,2,3 (TGF-β1,2, ... Regulation of clonal T-cell responses by IL-10-secreting islet-specific Tregs is dependent on cell-cell contact and operates by ... T-cells are potent suppressors of proinflammatory effector Th1 cells, of the type believed to mediate islet β-cell destruction ...http://diabetes.diabetesjournals.org/content/59/6/1451
The pGL3 constructs were transiently transfected into a 3T3-L1 cell line (American Type Culture Collection) under both normal ... and 2-h plasma insulin concentrations and rates of glucose disposal during the low-dose insulin infusion were log-transformed ... 3T3-L1 cells were maintained in standard growth medium: Dulbecco's modified Eagle medium (DMEM) (Gibco BRL) modified to contain ... For each cell extract, dual luciferase assays were performed in triplicate using the standard protocol (Promega), and light was ...http://diabetes.diabetesjournals.org/content/52/7/1864
The Regulation of Glucose-Excited Neurons in the Hypothalamic Arcuate Nucleus by Glucose and Feeding-Relevant Peptides |...
The dashed line represents 0% change (by definition, 2.5 mmol/l glucose). A and B: The data fit the following equation for a ... The cell suspension was centrifuged 4 min at room temperature at 1,000 rpm, and the pellet was resuspended with 1-1.5 ml zero- ... The data were initially transformed by adding an integer to give all positive values, as described by Winer (ref. 32). The data ... Whole-cell recordings in brain slices.. Immediately before use, brain slices were placed in a chamber (Warner Instruments) with ...http://diabetes.diabetesjournals.org/content/53/8/1959?ijkey=762636fc84c16529654aebf67a1f68dd5d76992f&keytype2=tf_ipsecsha
Genomic DNA extraction from lymphoblastoid cell lines.. Genomic DNA was extracted from lymphoblastoid cell lines using a DNeasy ... We examined Epstein-Barr virus-transformed lymphoblastoid cells from normal-weight subjects who were also heterozygous for the ... A: Genomic and cDNA sequences from the patient's lymphoblastoid cell line. The patient is heterozygous for the BDNF coding ... RNA from the proband's lymphoblastoid cell line consistently showed exclusively monoallelic expression confirming the ...http://diabetes.diabetesjournals.org/content/55/12/3366
SNUOT-Rb1 cells from the human retinoblastoma cell line established by our group (13) were maintained in RPMI 1640 medium ( ... The Saccharomyces cerevisiae BJ3501 strain was transformed using an expression vector for rhLK8, which was constructed to ... The cells were added to each Boyden chamber and incubated for 4 h at 37°C. After the incubation, cells were fixed and stained ... Cell viability assay.. HUVECs and SNUOT-Rb1 cells were treated with rhLK8 for 24 h. The medium was replaced with fresh medium ...http://diabetes.diabetesjournals.org/content/61/6/1599
Defects in β-Cell Ca2+ Dynamics in Age-Induced Diabetes | Diabetes
... i dynamics in the aging β-cell are also consistent with the observation that SERCA inhibitors transform a mixed oscillatory ... In line with this reasoning, the depolarizing effect on mitochondrial membrane potential induced by 1 μmol/L FCCP was decreased ... Mitochondrial signals drive insulin secretion in the pancreatic β-cell. Mol Cell Endocrinol 2012;353:128-137pmid:21784130. ... Defects in β-Cell Ca2+ Dynamics in Age-Induced Diabetes. Luosheng Li, Aleksandra Trifunovic, Martin Köhler, Yixin Wang, Jelena ...http://diabetes.diabetesjournals.org/content/63/12/4100