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*  How do you transform fibroblast cells for making cell lines?
... Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu Sat ... Next message: How do you transform fibroblast cells for making cell lines? ... Next message: How do you transform fibroblast cells for making cell lines? ... What stuck in my head is that resulting cell line(s) in many respects has very little little in common with the primary cells ...
http://www.bio.net/bionet/mm/methods/1997-December/063565.html
*  V79 cell line ?
Somatic Cell Gen 1979; 95:615- comments esatbl. after spontan. transform. of cells isolated from the lung of a normal Chinese ... Hi I work with a cell line we referre to in our lab as 'V79'. The technician who gave me the cells with this information ... V79 cell line ?. Roger C. Leemann leemann at imr.psi.ch Thu Oct 24 16:08:22 EST 1996 *Previous message: Stressproteins WORKSHOP ... How many different V79 cell lines are there? Is there a list on the web? Does anybody know the full name of 'Somatic Cell Gen' ...
http://www.bio.net/bionet/mm/cellbiol/1996-October/005562.html
*  Protocol for Northwestern analysis
Next message: Lipofection of a transformed human B cell line. * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] ...
http://www.bio.net/bionet/mm/methods/2002-April/092943.html
*  Fibroblast blues
... cell line (needless to say an important one) is growing poorly as it is barely ,able to attach to the plate and can't compete ... with these aggressive fibro- ,blasts (maybe they have been transformed?). Is there a miracle anti-fibro- ,blast agent? Is there ... simply transferring the cells to a new plate usually ,does the trick. Now I have a line of fibroblasts which refuses to die ( ... I have a hybridoma cell line contaminated with fibroblasts. Normally I don't ,have a problem eliminating these since they stick ...
http://www.bio.net/bionet/mm/immuno/1993-July/000454.html
*  Autofluorescence of endothelial cells
... primary human umbilical vein endothelial cells or a human papilloma virus transformed umbilical vein endothelial cell line. ... Autofluorescence of endothelial cells. T.Vink at med.ruu.nl T.Vink at med.ruu.nl Tue Dec 12 11:23:56 EST 1995 *Previous message ... Autofluoressence is observed without adding any fluorescent antibody and cannot be quenched by incubating the cells with 1 mg/ ... Autofluorescence of endothelial cells Date: Tue, 12 Dec 1995 16:23:56 GMT From: Tom Vink ,T.Vink at med.ruu.nl, Subject: ...
http://www.bio.net/bionet/mm/methods/1995-December/037989.html
*  Zbrafish] Express genes in ZF-1 cell line
... in Oakland University-I am writing to see if anybody know if we can clone genes in vector to transform into ZF cell line? Thank ... Zbrafish] Express genes in ZF-1 cell line. detroitriver via zbrafish%40net.bio.net (by LiuZijuan from gmail.com). Sat Mar 1 16: ...
http://www.bio.net/bionet/mm/zbrafish/2008-March/006313.html
*  abstracts re toxicity of polycyclic aromatic carbohydrates: Hubner: Murray 2004.05.15
Cell lines were established from six of these tumors and were tested for in vitro lysis by NK cells. Sensitivity of these cells ... The study supports a role for NK cells in the early detection and removal of transformed cells and points out the dangers of ... It was found that the mitochondria and cytosol of the cells contained 3-5 nmol/10(6) cells (approximately 15%) and 20-30 nmol/ ... T-cell-dependent) 144, 231, 42 and 27 units, respectively, and (TNP-LPS, T-cell-independent) 161, 406, 312 and 69 units, ...
http://www.bio.net/bionet/mm/toxicol/2004-May/003490.html
*  Overexpression of Eukaryotic proteins in E. coli
No line was better at producing MFP. Also, compared to the vector with no insert, ie. just the MBP, the expression of my ... Also, upon induction, the cells continue to grow even if they are making MFP, so the product isn't lethal. I have tried to ... I get expression at first after transforming the plasmid into the host, but as I subculture the little bugs, they decide to ... Does anyone have an idea as to: 1. Why should the amount of expression decline in cells grown without induction. (The vector ...
http://www.bio.net/bionet/mm/methods/1992-November/003314.html
*  MDCK Cell :Reply
Kang, I have not had that much experience with the MDCK cell line but have worked with a number of other cell lines and primary ... I'm not aware of the MDCK spontaneously transforming in vitro, fom epithelial to fibroblast. Have you had your cell cultures ... Doubling time of the cell is very long. , I want to knowf what happen to my cell!! , Could character of MDCK cell be changed by ... ¹Ì ¼± wrote: , , I culture MDCK cell to research toxicity mechaism of heavy metal. , MDCK cell is kidney epithelial cell and ...
http://www.bio.net/bionet/mm/cellbiol/1998-September/009407.html
*  Low concentration miniprep
The next culprit is overgrown plates with satellite colonies picked instead of transformed. -- Heidi ... please edit your Subject line so it is more specific than 'Re: Contents of Methods digest...' Today's Topics: 1. 2D gel ... Next message: Eukaryotic Cell Membrane Specific Vector * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] ...
http://www.bio.net/bionet/mm/methods/2009-January/103794.html
*  IEEE EMBS'95 Time-Freq, Wavelets, Wavelet Packets in Biomed Engr.
... retinal-ganglion cells, lateral-geniculate-nucleus cells in the thalamus, and striate-cortex neurons. We have also investigated ... the continuous wavelet transform as well as the orthogonal wavelet transform have been investigated. An original nonlinear ... A range- 2-D Doppler provides estimates of the transverse and line-of-sight flows as a function of range. By optimal image, we ... The phi-transform (non-separable, non-orthogonal), and (3) The hexagonal wavelet transform (non-separable). Multiscale features ...
http://www.bio.net/bionet/mm/neur-sci/1995-April/017845.html
*  The Methods October 1996 Archive by author
Can ssDNA transform competent cell ? wliu at receptor.pharm.uwa.edu.au *rat cDNA library help wudc at NIC.BMI.AC.CN *Eukaryotic ... IL-1 responsive cell lines Paul Brennan *Plasmid sequences (pEF) Paul Brennan *Harbor Bioproducts address? John Brennand *High ... splitting cells Dima Klenchin *cell clumping after electroporation Dima Klenchin *transfection cells of neural origin Dima ... why G418 with stable lines? Annette C. Hollmann *Fraction Collector Source Louis Hom *splitting cells Louis Hom *plasmid ...
http://www.bio.net/bionet/mm/methods/1996-October/author.html
*  The Methods January 1994 Archive by subject
Please show me the storage method of HL-60 cell line!.2 Brian Foley *Please show me the storage method of HL-60 cell line!.2 ... transfection of HUVE cells? L.Y.Chau *transformation with denatured plasmids unknown at dl.ac.uk *Transforming LINEAR PLASMID ... HELP! unusual cell growth Robert Kuhelj, IJS *HELP! unusual cell growth Brian Foley *HELP! unusual cell growth Marianna Max * ... cell sorting/analysis in the bay area ? Dov Shiffman *cells in the middle do not express the protein carlos h. pedemonte *cells ...
http://www.bio.net/bionet/mm/methods/1994-January/subject.html
*  The Methods October 1994 Archive by thread
... carcinoma cell line Volkert Clausen *Transfection of oesophageal (squamous cell)carcinoma cell line Irene Schauer * ... How do you transform aorta-smooth muscle cells? aeschba at fmi.ch *Extraction of DNA from agarose gels futers at biovax.leeds. ... Transfection of neuronal cell lines??? Michael Poidinger *Transfection of neuronal cell lines??? CARPENTER E. *Artifacts in ... Viral Cell Packaging line GP+E86 POLAKBM at WPOGATE.SLU.EDU *cold SSCP gli at UCDAVIS.EDU *PC-based plasmid map programs nicola ...
http://www.bio.net/bionet/mm/methods/1994-October/thread.html
*  The Methods December 1994 Archive by thread
HELP WITH TRANSFORM./COMP.CELLS!!! Darren Stauth *HELP WITH TRANSFORM./COMP.CELLS!!! Zhixiang Hu *HELP WITH TRANSFORM./COMP. ... human cell lines with high viability jxu at MIT.EDU *RNA / nothern blotting question Lana C Stallard *Bot Tox Affinity Column ... HELP WITH TRANSFORM./COMP.CELLS!!! bipin dalmia *HELP WITH TRANSFORM./COMP.CELLS!!! Duncan Clark *HELP WITH TRANSFORM./COMP. ... Source of B16 melanoma cells? Shane Albright *Source of B16 melanoma cells? Shane Albright *Wizard Preps: DNA Doesn't Freeze ...
http://www.bio.net/bionet/mm/methods/1994-December/thread.html