... are becoming an emerging resource for the development of eco-sustainable plastic degradation and recycling processes. In this ... are becoming an emerging resource for the development of eco sustainable plastic degradation and recycling processes. In this ... investigations on cell growth, enzyme production and monomers consumption. Process Biochem. 95, 81-90. doi: 10.1016/j.procbio. ... A flow cell quality control (SpotON Flow Cell Mk I R9 Version, FLO-Min 106D) was run prior to the start of sequencing. The ...

mTOR signaling in growth, metabolism, and disease. Cell (2017) 168(6):960-76. doi: 10.1016/j.cell.2017.02.004 ... The first process involves signaling from the mammalian target of rapamycin (mTOR) and the Sir2-like protein 1 (Sirt1), both ... This inhibits Sirt1 and activates mTOR to initiate protein production and cell growth while inhibiting autophagy (119, 120). At ... where the cell bodies of dopamine-producing neurons are located), the basal forebrain (where the cell bodies of acetylcholine- ...

Losmapimod improved the cell viability of pilocarpine-induced neuron cells. Down-regulating MAPK phosphorylation level ... A TdT-mediated dUTP nick-end labeling (TUNEL) assay was used to identify cell apoptosis. Hippocampal cytoarchitecture was ... TUNEL assay was used to indicate cell apoptosis. Hippocampal cytoarchitecture was visualized with Nissl staining. The secretion ... protected epilepsy by inhibiting hippocampal neuron apoptosis and enhancing hippocampal neuron cell viability. Losmapimod ...

However, experimental evidence suggests these cells are important for thymus function by either directly influencing T-cell ... However, experimental evidence suggests these cells are important for thymus function by either directly influencing T-cell ... Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T- ... cell development. Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic ...

Several studies have suggested that abnormal AS events were the primary element in the pathological process of cancer. However ... Several studies have suggested that abnormal AS events were the primary element in the pathological process of cancer. However ... event is a highly complex process that plays an essential role in post-transcriptional gene expression. ... event is a highly complex process that plays an essential role in post-transcriptional gene expression. ...

... the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question ... whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. In this review, we ... the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question ... whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. In this review, we ...

We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a ... Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency ... We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a ... In order to ensure high fidelity performance, we have implemented a high-speed microscope for in-process quality control, and ...

Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of ... Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of ... plays a crucial role in the musculoskeletal homeostasis and repair process. ... play a crucial role in the musculoskeletal homeostasis and repair process. ...

A four-track perspective for bottom-up synthetic cells ... The whole process would lead to a spontaneous growth-division ... Keywords: artificial cells, synthetic cells, protocells, bottom-up synthetic biology, cell-free systems, sciences of the ... Gaut, N. J., and Adamala, K. P. (2021). Reconstituting natural cell elements in synthetic cells. Adv. Biol. (Weinh). 5, ... Cho, E., and Lu, Y. (2020). Compartmentalizing cell-free systems: Toward creating life-like artificial cells and beyond. ACS ...

... and reduce vascular smooth muscle cell proliferation. All EETs are metabolized to the less active dihydroxyeicosatrienoic acids ... and reduce vascular smooth muscle cell proliferation. All EETs are metabolized to the less active dihydroxyeicosatrienoic acids ... it has been shown that EETs are critical for primary tumor growth and metastasis, by directly promoting cancer cell ... In addition, EETs play important roles in the regulation of angiogenesis and in the control of metabolic processes (Figure 2; ...

This study investigated the link between GM and the intrahepatic T cell receptor (TCR) immune repertoire (IR), and whether GM ... Furthermore, GM modulated hepatic stellate cell (HSC) activation via TCR IR-mediated intrahepatic immune milieu. Our study ... Furthermore, GM modulated hepatic stellate cell (HSC) activation via TCR IR-mediated intrahepatic immune milieu. Our study ... This study investigated the link between GM and the intrahepatic T cell receptor (TCR) immune repertoire (IR), and whether GM ...

... can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in ... Its cell-free supernatant (LAB-CFS) is rich in bioactive substances. This manuscript examined the antimicrobial efficacy of LAB ... LAB-CFS treatment significantly inhibited the bacterial growth, generation of eDNA (extracellular DNA), extracellular ... growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the S. aureus biofilm, ...

... the differences of vitreous protein profiles in PDR patients before and after a complete anti-vascular endothelial growth ... the differences of vitreous protein profiles in PDR patients before and after a complete anti-vascular endothelial growth ... NPEPPS is involved in proteolytic events essential for cell growth and viability. It is required for the proliferation of ... Sample processing refers to the method in our previous publication (12).. Sample Processing. VH samples were lysed according to ...

... disrupted tip-localized deposition of cell wall material, especially pectins, and inhibited pollen tube growth. Conversely, ... DGKs and LPPs into the PA regulated processes. LPPs inhibitors corroborated our previous data implying enhanced PA in the ... In contrast, reduction of PA levels compromised membrane trafficking and pollen tube growth but not early endocytosis. Also the ... Our results clearly demonstrate strikingly different roles of PA produced by PLDs and DGKs in pollen tube growth. ...

Among them, the sirtuin, AMP-activated protein kinase, mammalian target of rapamycin, p53, and insulin/insulin-like growth ... Among them, the sirtuin, AMP-activated protein kinase, mammalian target of rapamycin, p53, and insulin/insulin-like growth ... Aging is an inevitable process influenced by genetics, lifestyles, and environments. With the rapid social and economic ... Aging is an inevitable process influenced by genetics, lifestyles, and environments. With the rapid social and economic ...

Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line ... Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type TP53 and a ccRCC cell line ... Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma ( ... Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma ( ...

Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in ... Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in ... as these cells showed increased expression of genes that reflect stem cell attributes; whereas LREC in suprabasal epithelial ... as these cells showed increased expression of genes that reflect stem cell attributes; whereas LREC in suprabasal epithelial ...

... aim was to establish a live cell culture to enable the understanding of the cellular processes driving the non-malignant growth ... Conclusions: A spontaneous cell line-like cell culture with high percentage of stem cells has been established from a DIG tumor ... presence of cancer stem cells and the expression of stem cell markers. Cells cultured as 3D were embedded as tissue blocks. ... presence of cancer stem cells and the expression of stem cell markers. Cells cultured as 3D were embedded as tissue blocks. ...

Piericidins C7 and C8 lead to selective cytotoxicity on RG-E1A-7 cells and non-lethal growth inhibition on Neuro-2a cells. The ... Under hypoxia and oxidative stress, cells begin the process of angiogenesis through factors such as HIF-1α to maintain their ... Stopping the cell cycle at G2/M and induction of apoptosis by this compound in HCT-116 cells were other results of this study ( ... These therapies target the exact molecular pathway involved in causing cancer to inhibit the growth of cancer cells and limit ...

... cells and will discuss potential functions of vesicle-free miRNAs and how vesicle-free miRNAs regulate cell-to-cell ... in this way vesicle-free miRNA may regulate celltocell communication including the regulation of gene expression and cellular ... vesicle-free miRNA may regulate cell-to-cell communication including the regulation of gene expression and cellular signaling. ... Extracellular circulating miRNAs are also observed outside the cell, but their origin is poorly understood. Recently, miRNA has ...

Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects ... Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects ... on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. The focus of this article is in 3-fold: (1) ... on myogenic regulatory factors (MRFs), insulin growth factor-1, and heat-shock proteins. The focus of this article is in ...

Dysregulated autophagy in pancreatic β cells due to hyperglycemia, oxidative stress, and inflammation is associated with ... Dysregulated autophagy in pancreatic β-cells due to hyperglycemia, oxidative stress, and inflammation is associated with ... which is associated with activation of several processes such proliferation, cell growth, and glucose uptake. Suppression of ... class I and are consequently recognized by T cell receptors on CD8+ T cells. This results in T cell activation and β cell ...

... cells. The mice were divided into five groups: (1) tumor control; (2) lycopene (5 mg/kg); (3) sorafenib (30 mg/kg); (4) ... cells. The mice were divided into five groups: (1) tumor control; (2) lycopene (5 mg/kg); (3) sorafenib (30 mg/kg); (4) ... Raf to promote cancer cell proliferation, survival, and metastasis (41). The JNK pathway can mainly regulate cell growth, ... The MAPK pathways have been demonstrated to be involved in many biological processes in cells including the metastasis of ...

... a cellular transformation process required for trophoblast invasion and ultimately placentation. We show here that, like SRC-2 ... cell proliferation/motility, and programmed cell death. These results were confirmed by the demonstration that SRC-3 is ... level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell ... level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell ...

... community with the cell recovery necessary for further cultivation will allow for an efficient initial screening process. In ... fluorescence-activated cell sorting (FACS) for the rapid detection and collection of target cells. To implement this screening ... fluorescence-activated cell sorting (FACS) for the rapid detection and collection of target cells. To implement this screening ... that can visualize polyP granules while maintaining viability for the majority of the cells (,60%). The polyP positive cells ...

... expressed in mammalian organisms and its proteolytic action influences several physiological and pathological processes. This ... ADAM17 is a metalloprotease and disintegrin that lodges in the plasmatic membrane of several cell types and is able to cleave a ... wide variety of cell surface proteins. It is somatically ... several cell types and is able to cleave a wide variety of cell ... expressed in mammalian organisms and its proteolytic action influences several physiological and pathological processes. This ...

These organic conductors provide safe electrical stimulation of tissue while avoiding undesirable chemical reactions and cell ... These organic conductors provide safe electrical stimulation of tissue while avoiding undesirable chemical reactions and cell ... CH materials have been used to support the attachment and growth of PC12 cells, the interleukin-3 dependent pro-B cell line ( ... Finally, the encapsulated cells must form functional synaptic processes with the target neurons. These issues present great ...

This water-soluble B9 vitamin plays an essential role in cell renewal and cell growth. It functions as a coenzyme in the ... Consequently, we investigated the production process and concluded that folate degradation arises due to the harsh processing ... The processed pequis showed highly different results than the fresh and freeze-dried samples, with a maximum of 4.13 μg/100 g ... The worlds population is projected to reach nearly 10 billion by 2050, and with this growth comes the need for increased food ...

... growth and PHB production stopped when N, as a second nutrient, became growth-limiting. Finally, it was concluded that a ... growth and PHB production stopped when N, as a second nutrient, became growth-limiting. Finally, it was concluded that a ... It was found that C,P-limited PHB productivity was 5 times higher than for only C-limited growth and reached a maximal PHB ... It was found that C,P-limited PHB productivity was 5 times higher than for only C-limited growth and reached a maximal PHB ...

... such as transforming growth factor beta (TGF-beta), Vascular Endothelial Growth Factor (VEGF), and insulin-like growth factor 1 ... ESWT increases the expression of growth factors, such as transforming growth factor β(TGF-β), (VEGF), and insulin-like growth ... such as vascular endothelial growth factor (VEGF) and a crucial role of inflammatory elements and cytokines was demonstrated. ... such as vascular endothelial growth factor (VEGF) and a crucial role of inflammatory elements and cytokines was demonstrated. ...