XDream: Finding preferred stimuli for visual neurons using generative networks and gradient-free optimization | PLOS...
In: Advances in Neural Information Processing Systems; 2016. p. 658-666. *9. Yamane Y, Carlson ET, Bowman KC, Wang Z, Connor CE ... A Cortical Region Consisting Entirely of Face-Selective Cells. Science. 2006;311(5761):670-674. * View Article ... In: Advances in Neural Information Processing Systems; 2016. p. 3387-3395. *13. Ponce CR, Xiao W, Schade P, Hartmann TS, ... Advances in Neural Information Processing Systems 25; 2012. p. 1097-1105. *24. Zhou B, Lapedriza A, Khosla A, Oliva A, Torralba ...
Ancestral SARS-CoV-2, but not Omicron, replicates less efficiently in primary pediatric nasal epithelial cells | PLOS Biology
This study uses nasal epithelial cells from children and adults to show that the ancestral SARS-CoV-2 and Delta, but not the ... Omicron variant, replicate less efficiently in pediatric nasal epithelial cells. ... Real-time PCR was performed on generated cDNA with SYBER Green (Invitrogen) using QuantStudio 6 Flex Real-Time PCR System, an ... and 1-μg DNA-free RNA was reverse transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied ...
Down-Regulation of miR-101 in Endothelial Cells Promotes Blood Vessel Formation through Reduced Repression of EZH2 | PLOS ONE
Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional ... In vitro, the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through ... Cells. Human brain microvascular endothelial cells (HBMVECs; Cell Systems ACBRI-376) were cultured in EGM medium (Lonza). 293T ... with 50 nM of oligonucleotides or treatment with 5 µM DZNep cells were starved for 24 h and harvested in serum free medium. Per ...
HIV-1 Polymerase Inhibition by Nucleoside Analogs: Cellular- and Kinetic Parameters of Efficacy, Susceptibility and Resistance...
... that selection of drug resistance can occur in particular target cells. This finding could provide an explanation of how ... Our model also allows quantification of the cell specific factors affecting NRTI efficacy. Resistance development also changes ... typically derived from cell-free in vitro assays. These parameters can usually be precisely determined with standard errors . ... shifting the balance between viral clearance by the immune system and viral replication in favor of the immune system. For the ...
Holding Thermal Receipt Paper and Eating Food after Using Hand Sanitizer Results in High Serum Bioactive and Urine Total Levels...
... significant free BPA was transferred to their hands and then to French fries that were eaten, and the combination of dermal and ... However, free BPA is applied to the outer layer of thermal receipt paper present in very high (∼20 mg BPA/g paper) quantities ... Vinas R, Watson CS (2013) Bisphenol S disrupts estradiol-induced nongenomic signaling in a rat pituitary cell line: effects on ... The report identified that "decision makers may wish to consider alternative printing systems". Two of the papers screened for ...
Mendelian randomization analysis of plasma levels of CD209 and MICB proteins and the risk of varicose veins of lower...
... proteins reported in a previous hypothesis-free MR study. We conducted MR analysis using a fixed effects inverse-variance ... DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY ... Results of hypothesis-free 2SMR analysis for CD209 and MICB vs.. VVs obtained in our previous study. ... Suri is a Staff Physician at the VA Puget Sound Health Care System. Dr. Stanaways time was supported by the University of ...
Expansion microscopy using a single anchor molecule for high-yield multiplexed imaging of proteins and RNAs | PLOS ONE
uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues. ... Fixed cells and tissue slices were first pre-incubated with 100 mM sodium bicarbonate (pH = 8.5, DNase/RNase-free) twice for 15 ... Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of ... Cell culture and mouse brain tissues. HeLa cells were cultured in DMEM medium supplemented with 10% FBS and 1% penicillin- ...
Identifying causative mechanisms linking early-life stress to psycho-cardio-metabolic multi-morbidity: The EarlyCause project |...
... neuroendocrine system, inflammatome, and the gut microbiome. Life-course models will integrate the role of modifying factors as ... the cardiovascular and metabolic systems. The research will mainly focus on four biological processes including possible ... pancreatic cells), and blood immune system (peripheral blood mononuclear cells), in order to identify the molecular processes ... free fatty acids, glycerol, C reactive protein will be quantified with appropriate assays. Corticosterone (HPA-axis activation ...
Mapping C-Terminal Transactivation Domains of the Nuclear HER Family Receptor Tyrosine Kinase HER3 | PLOS ONE
... mutants were constructed to map minimal regions of transactivation potential using the Gal4 UAS-luciferase based system. These ... have been observed in primary tumor specimens and cancer cell lines for nearly two decades. Inside the nucleus, HER family ... cells were washed briefly with PBS, and fixed with 4% methanol-free formaldehyde for 15 min at room temperature. Cells were ... Cell Lines. The human breast cancer cell line MCF-7, human colorectal cancer (CRC) cell lines LoVo, CaCO2, and CHOK1 cells were ...
The Rsb Phosphoregulatory Network Controls Availability of the Primary Sigma Factor in Chlamydia trachomatis and Influences the...
Our results suggest a mechanism by which Chlamydia can tailor its growth rate to the conditions within the host cell. The ... C. trachomatis only replicates from inside a host cell, and has evolved to acquire a variety of nutrients directly from its ... in its ability to survive these immunological stress situations by slowing growth and development until conditions in the cell ... Images of plaques were captured from beneath the well using a GelDoc-It Imaging system (Bioimaging Systems) using the exact ...
Impact of HIV-1 Backbone on Neutralization Sensitivity: Neutralization Profiles of Heterologous Envelope Glycoproteins...
FF and LucR activity can be both measured simultaneously in the same infected cells using the Dual-Glo Luciferase Assay System ... PCR products obtained were gel purified with S.N.A.P. UV-free gel purification kit (Invitrogen) and finally quantified on 0.8% ... using TZM-bl cells, a CD4+T-cell line (A3R5) [24], and PBMC; both viral constructs infected all target cell types and exhibited ... which includes both HIV-1 target cells (CD4+ T cells) and anti-HIV-1 effector cells, measures several types of antibody ...
Assessment of a cellular host response test to risk-stratify suspected COVID-19 patients in the Emergency Department setting |...
Green band patients had more hospital free days (median 23 (Q1-Q3 20-25) than yellow (median 22 [Q1-Q3 0-23], p , 0.05) and red ... The system that is the subject of this study is for investigational use only (not yet cleared for commercial use) and is ... p-values were obtained from an unpaired two-sample Welchs t-test (except for hospital free days, where the Mann-Whitney U was ... Hydrodynamic stretching of single cells for large population mechanical phenotyping. Proc Natl Acad Sci U S A 2012; 109: 7630- ...
A Small Molecule p75NTR Ligand, LM11A-31, Reverses Cholinergic Neurite Dystrophy in Alzheimer's Disease Mouse Models with Mid-...
4A-E). The number of p75NTR-stained cells increased slightly but this difference was not statistically significant (Fig. 4F). ... Free-floating sections (every 8th section in a 1 to 16th series) were processed for immunohistochemical localization of choline ... 4. Ibanez CF, Simi A (2012) p75 neurotrophin receptor signaling in nervous system injury and degeneration: paradox and ... Thus, p75NTR is present in abundance on the cells of interest in this study, showing feasibility of the ligand acting through ...
Functional Dissection of Sugar Signals Affecting Gene Expression in Arabidopsis thaliana | PLOS ONE
... based on stem-cell-like Arabidopsis suspension culture. The cells were grown in a hormone-free medium and were sustained on ... This suggested that sugar-responsiveness of genes identified in the cell culture system was not biased toward heterotrophic ... Conclusions Altogether, our research strategy, using a combination of cell culture and whole plants, has provided an ... Methodology/Principal Findings To bypass those biases, we have developed a novel biological system, ...
Hands-Free Image Capture, Data Tagging and Transfer Using Google Glass: A Pilot Study for Improved Wound Care Management | PLOS...
In the comparison between SnapCap and Epic Haiku, the SnapCap System was preferred for sterile image-capture technique, Z(16 ... Preferred hands-free navigation features include barcode scanning for patient identification, Z(15) = -3.873, p , 0.001, r = ... In a pilot study with wound care nurses at Stanford Hospital (n=16), we (i) examined feature preferences for hands-free digital ... The studys results have contributed to the future implementation of new features aimed at enhancing mobile hands-free digital ...
PLOS Medicine
Format-free initial submission. PLOS Medicine uses the initial submission process, allowing authors to quickly submit to the ... In the submission system, enter only the preferred or primary affiliation. Author affiliations will be listed in the typeset ... Describe the study participants or what was studied (e.g., patient population, cell lines; be as specific as possible, ... Format-free full submission. If you receive an initial decision from the journal committing to peer review, you will be asked ...
Personalized Mortality Prediction Driven by Electronic Medical Data and a Patient Similarity Metric | PLOS ONE
With the increasing adoption of electronic medical record (EMR) systems, our novel medical data analytics contributes to ... study provides crucial empirical evidence for the promising potential of personalized data-driven decision support systems. ... white blood cell count, serum glucose, serum HCO3, serum potassium, serum sodium, blood urea nitrogen, and serum creatinine. ... The clinical part of MIMIC-II is a relational database and interested researchers are free to write their own SQL queries to ...
Ecology Drives the Worldwide Distribution of Human Diseases | PLOS Biology
Black cells are highly predictable presences, whereas red cells are unexpected presences. ... The vast majority of studies exploring the latitudinal species diversity gradient have focused on free-living organisms, such ... We assessed the degree of nestedness of the system using two different, but complementary, analysis programs: (1) Nestedness ( ... As we move away from the corner, where cells are most likely to be occupied, unexpected absences and presences begin to appear ...
Chemical synthesis | PLOS ONE
Cellular apoptosis and cell cycle arrest as potential therapeutic targets for eugenol derivatives in Candida auris Hammad Alam, ... Green synthesised AuNps using Ajuga Bracteosa extract and AuNps-Free supernatant exhibited equivalent antibacterial and ...
Identification and Localization of the Cyclic Nucleotide Phosphodiesterase 10A in Bovine Testis and Mature Spermatozoa | PLOS...
The objective of this study was to identify the PDE10A isoforms expressed in the testis and germ cells, and to determine the ... information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. ... with the Expand Long Template PCR System (Roche) as DNA polymerase. A nested amplification was done replacing the gene specific ... Free Radic Biol Med. 2000;29(6):522-36. pmid:11025196 * View Article ...
Effects of Lithium and Valproic Acid on Gene Expression and Phenotypic Markers in an NT2 Neurosphere Model of Neural...
Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated ... or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. In contrast, exposure to VPA resulted in ... Neurofilament M and GFAP with significant decreases in both G2/M phase cells and cell number. This neurosphere model might ... changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. ...
Identification of a Novel Drug Lead That Inhibits HCV Infection and Cell-to-Cell Transmission by Targeting the HCV E2...
281816 blocked the early and late steps of cell-free HCV entry and also abrogated the cell-to-cell transmission of HCV. ... and the best binders were subsequently tested to identify compounds that inhibit the infection of Huh-7 cells by HCV. One ... Cell binding Is the Subject Area "Cell binding" applicable to this article? Yes. No. ...
Effect of Ku80 Deficiency on Mutation Frequencies and Spectra at a LacZ Reporter Locus in Mouse Tissues and Cells | PLOS ONE
These results indicate that Ku80-dependent repair of DNA damage is predominantly error-free with the effect of alternative more ... To investigate the effect of inactivated NHEJ on spontaneous mutation frequencies and spectra in vivo and in cultured cells, we ... However, after such treatment, Ku80-deficient cells did show an increased number of persistent DNA damage foci. ...
Brazil's Market for Trading Forest Certificates | PLOS ONE
Also, there is a need to couple the CAR to land tenure systems in order to verify property boundaries. All these efforts will ... Concurrent allocation of crops at raster cell resolution is a function of crop aptitude and profitability (Figures C and D in ... In this way, the model is free to allocate future croplands based solely on crop suitability, regardless of the need to restore ... In addition, transactions and sunk costs are much lower for both landowners and the MRV system. Oversupply would not be a ...
CK1ε Is Required for Breast Cancers Dependent on β-Catenin Activity | PLOS ONE
We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner ... Furthermore, we demonstrate that breast cancer cells that harbor activated β-catenin activity exhibit enhanced sensitivity to ... we performed RNAi-based loss-of-function screens in breast cancer cell lines in which we had characterized β-catenin activity. ... as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a ...
Phylogenomic Analyses Support Traditional Relationships within Cnidaria | PLOS ONE
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built ... Mitochondrial genomes of anthozoa (Cnidaria). Prog Cell Cycle Res. 1995;5: 149-153. * View Article ... canal systems in the polyps, musculature organized in bundles of ectodermal origin, rhopalia or rhopalia-like structures, and ... While medusozoans are often thought of as being characterized by the presence of a free-swimming medusa stage, this is far from ...
PLOS Complex Systems
PLOS provides a free, simple-to-use tool called PACE to help you review and prepare your figures for submission and achieve ... We are grateful to staff at the Journal of Cell Biology (Rockefeller University Press) for their help in establishing these ... PLOS Complex Systems publishes research of broad significance that untangles the complex systems at the heart of the world we ... PLOS Complex Systems waives all formatting requirements until your manuscript has received a provisional Editorial Accept ...
Context-dependent reversal of odorant preference is driven by inversion of the response in a single sensory neuron type | PLOS...
Molecular topography of an entire nervous system. Cell. 2021;184:4329-47. pmid:34237253 * View Article ... These steps ensured that the arena was bubble-free prior to loading the worms and stimulus reservoirs. Buffer and stimulus ... ASH responds partly cell-autonomously to hexanol.. (A) Behaviors of wild-type or a strain expressing GCaMP3 in ASH under the ... Chemosensory cell function in the behavior and development of Caenorhabditis elegans. Cold Spring Harb Symp Quant Biol. 1990;LV ...
Population Health Impact and Cost-Effectiveness of Tuberculosis Diagnosis with Xpert MTB/RIF: A Dynamic Simulation and Economic...
Seven HIV subdivisions were created, defined by CD4 cell count (,350 cells/µl, 200-350 cells/µl, and ,200 cells/µl) and by ... Health System Costs of Introducing Xpert. Figure 4 shows the additional annual costs associated with the Xpert scenario ... 2009) Timing of initiation of antiretroviral therapy in AIDS-free HIV-1-infected patients: a collaborative analysis of 18 HIV ... 200 cells/µl and then extended to include those with a CD4 count in the range 200-350 cells/µl from 2010 onward, consistent ...
Extracellular Localization of the Diterpene Sclareol in Clary Sage (Salvia sclarea L., Lamiaceae) | PLOS ONE
Guo Z, Severson RF, Wagner GJ (1994) Biosynthesis of the diterpene cis-abienol in cell-free extracts of tobacco trichomes. Arch ... Guo Z, Wagner GJ (2004) Biosynthesis of labdenediol and sclareol in cell-free extracts from trichomes of Nicotiana glutinosa. ... the cell wall and the cuticle to later crystallize on its surface. In a typical epidermal cell, the cuticle is made of an ... the stalk-cells, and on the pavement cells that surround the glandular trichomes. This latter mechanism has been demonstrated ...