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*  GW24-e2975 Over-expression of calpastatin aggravates in vitro and in vivo cardiotoxicity induced by doxorubicin | Heart
Cardiomyocytes apoptosis was assayed by caspase-3 activity assay, DNA fragmentation ELISA and Annexin-V staining. Calpain ... Volume 99, Issue Suppl 3 *GW24-e2975 Over-expression of calpastatin aggravates in vitro and in vivo cardiotoxicity induced by ... and a concomitant reduction in GSK-3 beta phosphorylation (Ser9) in doxorubicin-treated cardiomyocytes. Inhibiting AKT further ...
http://heart.bmj.com/content/99/Suppl_3/A62.3
*  Glia2006 - Uni Oldenburg
MG-132-induced cell death, DNA-fragmentation, and caspase activation could be prevented by the broad caspase inhibitor zVAD-fmk ... Concomitantly, death-related caspases 3 and 9 were activated and poly(ADP-ribose)-polymerase cleavage occurred. ...
https://www.uni-oldenburg.de/en/neurosciences/molecular-neurobiology/publications/glia2006/
*  Photodynamic Treatment of Oral Squamous Cell Carcinoma Cells with Low Curcumin Concentrations
Curcumin triggers apoptosis via upregulation of Bax/Bcl-2 ratio and caspase activation in SW872 human adipocytes. Mol Med Rep. ... Caspase-3 cleavage is induced by curcumin-VIS treatment and camptothecin. HN cells were either cultivated in control medium (A ... Immunhistochemical analysis of cleaved caspase-3. HN cells were seeded in a cell density of 2x 104 cells/ml on glass cover ... 9) showed a concentration dependent increase of cleaved caspase-3 positive cells 4h after treatment with 1µg/ml (Fig. 9E) and ...
http://www.jcancer.org/v08p1271.htm
*  Dependence receptor TrkC is a putative colon cancer tumor suppressor | PNAS
caspase-3. *genetic. *epigenetic. Footnotes. *. ↵1A.-L.G. and G.I. contributed equally to this work. ... Patrick Mehlena,2,3. *. aApoptosis, Cancer and Development Laboratory, Equipe labellisée "La Ligue," LabEx DEVweCAN, Centre de ... 3To whom correspondence should be addressed. E-mail: patrick.mehlen{at}lyon.unicancer.fr. ... 3To whom correspondence should be addressed. E-mail: patrick.mehlen{at}lyon.unicancer.fr. ...
https://www.pnas.org/content/early/2013/01/16/1212333110.abstract?maxtoshow=&HITS=10&hits=60&RESULTFORMAT=1&andorexacttitle=and&andorexacttitleabs=and&fulltext=BioVision&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=match&fdate=1/1/2013&tdate=1/31/2013&resourcetype=HWCIT
*  Medical Xpress - immune tolerance
hCG reduces caspase-3-dependent IL-16 expression. (HealthDay)-Human pregnancy hormone chorionic gonadotropin (hCG) triggers ... immune suppression by attenuating the processing and release of Caspase-3-dependent interleukin (IL)-16, according to a study ...
https://medicalxpress.com/tags/immune+tolerance/
*  Plus it
2010) Activation of caspase-9, but not caspase-2 or caspase-8, is essential for heat-induced apoptosis in Jurkat cells. J Biol ... caspase-8 then activates caspase-3, which is responsible for most of the morphologic and biochemical manifestations of ... This could theoretically occur through caspase-8 and/or caspase-9 activation independently of the DISC and the apoptosome, ... 2009) Caspase-9 activation by the apoptosome is not required for fas-mediated apoptosis in type II Jurkat cells. J Biol Chem ...
http://molpharm.aspetjournals.org/content/83/1/245
*  Comparative effects of methylprednisolone and tetracosactide (ACTH 1-24 ) on ischemia/reperfusion injury of the rabbit spinal...
Members of the cysteine protease family known as caspases actuate apoptosis. Caspase-3 is an interleukin-converting enzyme and ... Antibodies specific for caspase-3 had been pre-coated onto a microplate. Standards and samples were pipetted into the wells, ... Tissue caspase-3 analyses. There was a statistically significant contrast between the control and the ischemia groups in their ... Tissue caspase-3 activities. Caspase-3 activity was measured using an ELISA kit (Cusabio, Hubei, China). The ELISA procedures ...
https://www.termedia.pl/Comparative-effects-of-methylprednisolone-and-tetracosactide-ACTH-1-24-on-ischemia-reperfusion-injury-of-the-rabbit-spinal-cord,19,29348,1,1.html
*  An anticancer drug suppresses the primary nucleation reaction that initiates the production of the toxic Aβ42 aggregates linked...
... caspase-9 is an initiator enzyme that mediates apoptotic pathways after mitochondrial damage and activates effector caspases, ... Caspase-3 cytotoxicity assay. Aβ42 oligomers are known to be toxic to cells leading to apoptosis and to cellular death. ... C to F) Levels of activated caspase-3 as an indicator of the cytotoxic effects of Aβ42 species on a human neuroblastoma cell ... Caspase-3/7 activity was measured using the Apo-ONE Homogeneous Caspase-3/7 Assay (Promega). The fluorogenic caspase-3/7 ...
https://advances.sciencemag.org/content/2/2/e1501244?ijkey=a160ee16779f16f71ee0a92590cfeeb5309554df&keytype2=tf_ipsecsha
*  SM22α-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and...
C) Apoptosis of HBVPs in response to serum deprivation using caspase 3/7 assay. Apoptosis in HBVPs transfected with SiC, SiBR1A ... Cell proliferation (MTT) and apoptosis (caspase 3 and 7 activity). Forty-eight hours following transfection in 0.1% FBS, ... as assessed by caspase 3 and 7 activities (Fig. 8C), without affecting cell proliferation as assessed by the MTT assay (data ... after which apoptosis was assessed by measuring caspase 3 and 7 activity using the Caspase 3/Caspase 7 Luminescent Assay Kit ( ...
http://dev.biologists.org/content/135/17/2981
*  Renal tubular epithelial cell apoptosis is associated with caspase cleavage of the NHE1 Na+/H+exchanger | Renal Physiology
... broad-spectrum peptide caspase inhibitor z-VAD-fmk or the peptide caspase-3 inhibitor z-DEVD-fmk. Whole cell lysates were ... To directly assess whether NHE1 is a caspase target, we incubated in vitro-translated cNHE1 with recombinant caspase-3 in the ... and cell shrinkage promote inexorable RTC apoptosis by optimizing pHi for further caspase activity and by bringing caspases in ... indicating that NHE1 is degraded by caspases. Recombinant caspase-3 cleaved the in vitro-translated NHE1 cytoplasmic domain ...
http://ajprenal.physiology.org/content/284/4/F829.long
*  Tissue Staining Reagents
Invitrogen™ Molecular Probes™ CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent Fluorogenic, no-wash indicator for live- and ... Invitrogen™ Molecular Probes™ TSA™ Kit 3, with HRP-Goat Anti-Mouse IgG and Alexa Fluor™ 546 Tyramide Tyramide signal ...
https://www.fishersci.ca/ca/en/products/I9C8K9O9/tissue-staining-reagents.html
*  Effect of freezing-thawing proces... preview & related info | Mendeley
... first part of our work to study the effect of cryopreservation on the human sperm DNA integrity and the activation of caspase 3 ... Activated caspase 3 levels were measured in spermatozoa using fluorescein-labeled inhibitor of caspase (FLICA). ... it had no effect on caspase 3 activation (p= 0.3). We conclude that oxidative stress plays a major role in inducing sperm ... Cryopreservation led to a significant increase in sperm DNA fragmentation, DNA oxidation and caspase 3 activation (p, 0.01). ...
https://www.mendeley.com/research-papers/effect-freezingthawing-process-quercetin-human-sperm-survival-dna-integrity/
*  Products in Assay Kits, Biotium on Thomas Scientific
found in: CellBrite™ Cytoplasmic Membrane Labeling Kits, XTT Cell Viability Assay Kit, NucView® 488 Caspase-3 Assay Kit for ... Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit Biotium. Caspase-3 is an active cell-death protease involved in the ... NucView® 488 Caspase-3 Assay Kit for Live Cells Biotium. NucView® 488 Caspase-3 substrate is a novel cell membrane-permeable ... Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit Biotium. Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit is specifically designed ...
https://www.thomassci.com/nav/cat1/assaykits/manufacturer/biotium/0
*  Immunomodulatory role of Emblica officinalis in arsenic induced oxidative damage and apoptosis in thymocytes of mice | BMC...
Increased activity of caspase-3 linked with apoptosis assessed by the cell cycle analysis and annexin V/PI binding was also ... Co-treatment with arsenic and amla decreased the levels of lipid peroxidation, ROS production, activity of caspase-3, apoptosis ... Mice were exposed to arsenic (sodium arsenite 3 mg/kg body weight p.o.) or amla (500 mg/kg body weight p.o.) or simultaneously ... Effect on caspase-3 activity in thymus of mice. Figure 7 demonstrate the effect of arsenic and co-treatment of arsenic and amla ...
https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-193
*  Plus it
TNF-α induces caspase-3 activation, GSH efflux, and ERK1/2 phosphorylation in cftr+/+ (C) and cftr−/− (D) cell lines. A: effect ... A: effect of Cl− channel blockers on STS-induced caspase-3 activity. cftr+/+ and cftr−/− cell lines were exposed for 6 h to STS ... AVD and caspase-3 activity were inhibited in the presence of DIDS, CFTRinh-172, PD98059, and NAC. Moreover, in the cftr−/− cell ... F: effects of l-buthionine-S,R-sulfoximine (BSO) on caspase-3 activation in cftr+/+ cell lines. cftr+/+ cells were exposed for ...
http://ajprenal.physiology.org/content/298/2/F435
*  Most Accessed Articles ::: Current Computer-Aided Drug Design
Molecular Docking Analysis of Caspase-3 Activators as Potential Anticancer Agents. , 2019; 15(1): 55-66.. Sushil K. Kashaw*, ... 2016; 12(3): 241-250.. Biplab De, Indrani Adhikari, Ashis Nandy, Achintya Saha and Binoy B. Goswami. DOI: 10.2174/ ...
https://benthamscience.com/journals/current-computer-aided-drug-design/most-accessed-articles/
*  Hyaluronic acid in the treatment of cancer - Bioniche Life Sciences Inc.
... caspase inhibitors, RNA, antisense RNA, DNA, antisense DNA, bacterial extracts, bacterial DNA, bacterial DNA-bacterial cell ... caspase activators, apoptosis inducers, cyclin inhibitors, CDK inhibitors, stable triple helix forming agents, genetically ... EXAMPLE 3. Preparation of HA of ≦5.0×105 Da. HA of ≦5.0×105 Da was prepared from HA of 5.0-7.5×105 Da by digestion with ... PC-3. LNCaP and Du-145 human prostate cancer cells were incubated with 8.0 μg/ml of 5.0-7.5×105 Da HA or of ,1.5×104 Da HA ( ...
http://www.freepatentsonline.com/7125858.html
*  p53 Modulation as a Therapeutic Strategy in Gastrointestinal Stromal Tumors
To this end, we studied nutlin-3, an inhibitor of the p53 antagonist MDM2, and RITA, a putative p53 activator, in GIST cell ... Nutlin-3 effectively induced p53 at therapeutically relevant levels, which resulted in moderate antiproliferative effects and ... Analyzing a series of 62 mostly untreated, localized and metastatic GIST we detected a low rate (3%) of inactivating p53 ... cell lines GIST882 and GIST-T1 were shown to harbor defective p53 and therefore failed to respond to nutlin-3 treatment. RITA ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0037776
*  'caspase 1' Protocols and Video...
Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction', 'A Simple Fluorescence ... caspase 1' include 'Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization ... Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation', 'The Ex Vivo Colon Organ ... Caspase 1: A long pro-domain caspase that has specificity for the precursor form of Interleukin-1beta. It plays a role in ...
https://www.jove.com/keyword/caspase+1
*  Shikonin | GreenMedInfo | Substance | Natural Medicine | Alternative
Pharmacological Actions : Antiproliferative , Apoptotic, Bcl-2 protein down-regulation, Caspase-3 Activation, Caspase-8 ... This study concludes that shikonin causes apoptosis via a p73-related, caspase-3-dependent pathway.Jul 29, 2015. ... Shikonin induced the generation of ROS as well as caspase 3-dependent apoptosis.May 30, 2016. ...
http://www.greenmedinfo.com/substance/shikonin
*  KAKEN - Research Projects | Planning-Coordination Committe on Special Cancer Research (KAKENHI-PROJECT-09042003)
... dependent endonuclease caspase-3 and sterss-activated protein kinases.' Experimental Cell Research. 239. 411-422 (1998). *. ... dependent endonuclease caspase-3,and sterss-activated protein kinases.' Experimental Cell Research. 239. 411-422 (1998). *. ... roles of Ca2+/Mg2+-dependent endonuclease.caspase-3.and stress-activated protein kinases.Axil, a member of the Axin family, ... Publications] Ikeda,S.: 'Axin,a negative regulator of the Wnt signaling pathway,forms a complex with GSK-3 β and β-catenin and ...
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09042003/
*  Biotium Product Catalog | Fluorescence-Based Reagents & Kits
NucView® 488 Caspase-3 Assay Kit for Live Cells Sizes: Trial size (25 assays), 100 assaysCatalog #: 30029, - 30029-TView all ... ViaFluor® Cell Proliferation , Live-or-Dye™ Fixable Viability , NucView® Caspase-3 Substrates ...
https://biotium.com/products/?item=10111-2
*  MicroRNA miR-34 Inhibits Human Pancreatic Cancer Tumor-Initiating Cells
Caspase activation in the transfected MiaPaCa2 cells was determined following the instructions of a Caspase-3 activation assay ... Caspase-3 activation assay was carried out as described in in Materials and Methods. Fold increase of fluorescence signal was ... Relative caspase-3 activation was calculated by normalizing the fluorescence signal in each treated sample with that of the NC ... miR-34 restoration inhibits MiaPaCa2 cell clonogenic growth and leads to caspase-3 activation and apoptosis. After validating ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0006816
*  Patent US7312090 - Label-free methods for performing assays using a colorimetric resonant ... - Google Patents
... an effector caspase, is the most studied of mammalian caspases because it can specifically cleave most known caspase-related ... Caspases (Cysteine-requiring Aspartate protease) are a family of proteases that mediate cell death and are important in the ... The caspase 3 assay is based on the hydrolysis of the 4-amino acid peptide substrate NHS-Gly-Asp-Glu-Val-Asp p-nitroanilide ( ... A Caspase-3 protease inhibitor assay was performed to demonstrate the biosensor's ability to measure the presence and cleavage ...
http://www.google.com/patents/US7312090?dq=6,460,050
*  Simplifying iPSC Work Flow with IncuCyte Live Content Imaging System
Applying IncuCyte® Apoptosis Assay for the Fluorescent Detection of Caspase-3/7 Activation or Phosphatidylserine ... Applying IncuCyte® Apoptosis Assay for the Fluorescent Detection of Caspase-3/7 Activation or Phosphatidylserine ... A Sartorius CompanyOct 3 2016. Introduction. Induced pluripotent stem cells (iPSCs) are characterized by the ability to self- ... Figure 3. Monitor cell health during reprogramming. Neonatal foreskin fibroblasts imaged just before (A) and 48 hours after (B ...
https://www.news-medical.net/whitepaper/20161003/Simplifying-iPSC-Work-Flow-with-IncuCyte-Live-Content-Imaging-System.aspx