Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway - Volume 8, Number 12-December 2002...
Caspase-3 (Pharmingen, San Diego, CA), caspase-8 (FADD-like interleukin-1 beta-converting enzyme) and caspase-9-like Mch6 (MBL ... The DN caspase-9 specifically blocked the cleavage of pro-caspase-9 in pcWNV-Cp-DJY and pcWNV-CpWT cotransfected cell lysates ... The cell lysates (100 μg/100 μl protein) were incubated with specific substrate Ac-DEVD-AMC for caspase-3, IETD-pNA for caspase ... Furthermore, to confirm that this apoptosis-induction pathway is through caspase-9, a domant negative (DN) caspase-9 construct ...
Volume 170 Issue 4 | Radiation Research
In the receptor-mediated pathway, CHRC5 cells exhibited higher levels of c-FLIP and lower caspase 8 and caspase 10 activation ... Similar levels of caspase 3 activation and cleavage of caspase substrates occurred, showing that overall, CHRC5 cells are not ... To assess this possibility, female wild-type and Apoe−/− mice were irradiated at 6 months of age with 10 Gy 137Cs γ rays and ... 1010 M−1 s−1, respectively. By contrast, the reaction between e−aq and MV· is too slow to affect G(MV· ). On the basis of these ...
Mucosa-Associated Lymphoid Tissue Lymphomas (MALTomas) Clinical Presentation: History and Physical Examination
Mucosa-Associated Lymphoid Tissue Lymphomas (MALTomas): Practice Essentials, Pathophysiology, Etiology
Genetic errors of the human caspase recruitment domain-B-cell lymphoma 10-mucosa-associated lymphoid tissue lymphoma- ... The survival rate for stage IE disease is 93% at 5 years and 58% at 10 years. [20] Long-term responses to anti-H pylori ... by interferon gamma and the production of IL-10 by type 2 THCs, which inhibits type 1 THCs. ... Patients Treated for Gastric Mucosa-Associated Lymphoid Tissue Lymphoma by Helicobacter pyloriEradication and Followed for 10 ...
Frontiers | Astragalin: a food-origin flavonoid with therapeutic effect for multiple diseases
... caspase-6, caspase-7, caspase-8, caspase-9, P53, and Bax and decreased cleaved caspase-3 and Bcl-2. In addition, AG also ... AG treatment increased the expressions of caspase-8, caspase-9, caspase-3, PARP, Bad, Bax, and Fas and decreased Bcl-xl and Bcl ... AG administration also upregulated the expression of caspase-3, caspase-9, and Bax and decreased Bcl-2, whereas AG did not ... cleaved caspase-9, and caspase-3, and down-regulating procaspase 9, cyclin D1, Mcl-1, and SOX10. Notably, SOX10 overexpression ...
Small compound 6‑O‑angeloylplenolin induces caspase‑dependent apoptosis in human multiple myeloma cells
6-OAP induces caspase-dependent apoptosis of multiple myeloma (MM) cells. (A) U266, MM.1S and RPMI 8226 cells were treated with ... Small compound 6‑O‑angeloylplenolin induces caspase‑dependent apoptosis in human multiple myeloma cells. *Authors: *Ying Liu ... The activation of the effector caspases was also analyzed and 6-OAP was found to induce casp-3 activation, followed by PARP ... Nicholson DW: Caspase structure, proteolytic substrates and function during apoptotic cell death. Cell Death Differ. 6:1028- ...
Výpis publikací
... of TRAIL in prostate cancer cells through Bid-dependent stimulation of mitochondrial apoptotic pathway but not caspase-10. Plos ... In 18th world congress on advances in oncology and 16th international symposium on molecular medicine, 10.-12. October 2013, ... In 53nd European Congress of the European Societies of Toxicology, 10-13. September 2017, Bratislava, Slovakia. 2017. ... In The Student Scientific Conference on Biotechnology and Biomedicine, 10.-12. April 2013, Brno, Czech Republic. 2013. ...
Caspase 3D structures - Proteopedia, life in 3D
Mouse CASP12(Caspase 12) ELISA Kit - Hiv Pharmacogenomics
Mouse CASP12(Caspase 12) ELISA Kit. Mouse CASP12(Caspase 12) ELISA Kit. To Order Contact us: [email protected] ... Description: A sandwich ELISA kit for detection of Caspase 12 from Mouse in samples from blood, serum, plasma, cell culture ... Description: A sandwich ELISA kit for quantitative measurement of Rat CASP12 (Caspase 12) in samples from Serum, Plasma, Cell ... Description: A sandwich CLIA kit for quantitative measurement of Rat CASP12 (Caspase 12) in samples from Serum, Plasma, Cell ...
Differential Expression of Apoptotic Protease-Activating Factor-1 and Caspase-3 Genes and Susceptibility to Apoptosis during...
Active caspase-9, in turn, cleaves and activates downstream caspases, including caspase-3. Therefore, we incubated cell-free ... Active caspase-9, in turn, activates executioner caspases-3 and -7. Activated caspase-3 is required for the activation of four ... leads to activation of caspase-9 (Li et al., 1997). Active caspase-9, in turn, activates caspase-3. ... Assay for caspase activity. Aliquots of cytosolic extracts (20 μg of protein in 100 μl of caspase-3 assay buffer) were mixed ...
PDF) Efficacy of dietary hempseed oil in patients with atopic dermatitis
... but activities of both caspase-9 (a marker of the intrinsic pathway) and caspase-3 (an executioner caspase) were significantly ... Caspases of the intrinsic and extrinsic pathways of apoptosis, Bcl-2 and Bax polypeptide levels, and plasma membrane neutral ... Caspase-8/10 activity (a marker of the extrinsic pathway) was not affected by either aging or dietary fat, ... The results span 10 years of proficiency testing and cover a range of plastic materials tested using a variety of time and ...
Viruses | Free Full-Text | Modulation of Apoptotic Pathways by Human Papillomaviruses (HPV): Mechanisms and Implications for...
Overexpression of anti-apoptotic proteins such as Bcl-2 or Bcl-XL cannot stop the cleavage of caspase-8 and caspase-3, and ... The apoptosome is analogous in function to the DISC, and mediates the activation of caspase-9, which in turn activates caspase- ... The active caspase-9 then cleaves and activates caspase-3, which induces the cleavage of additional substrates, such as PARP ... which then cleaves and activates executioner caspases such as procaspase-3/7. The active caspase-3/7 can cleave many different ...
Rat CASP14 (Caspase 14) CLIA Kit | G-EC-01800 | Gentaur Clia Kits
Caspase 14) CLIA Kit from Gentaur Clia Kits. Cat Number: G-EC-01800. USA, UK & Europe Distribution. ... Rat CASP14 (Caspase 14) CLIA Kit , G-EC-01800. Rating * Select Rating. 1 star (worst). 2 stars. 3 stars (average). 4 stars. 5 ... Rat CASP14 (Caspase 14) CLIA Kit , G-EC-01800 , Gentaur Clia Kits ...
Blebs produced by actin-myosin contraction during apoptosis release damage-associated molecular pattern proteins before...
Caspase activity was determined from equal cell numbers using Caspase-Glo 3/7 kits (Promega, Southampton, UK) according to ... d) Caspase 3/7 activity for each treatment relative to basal level (n=3). Inset shows western of ROCK1 cleavage time course in ... LDH, caspase activity and TNFα measurements. LDH activity was measured with Roche cytotoxicity detection kit according to ... Similarly, cleavage of the caspase substrate ROCK1 in TNFα/CHX-treated cells was not affected by Y27632 (Figure 3d inset). ...
James Zou's Profile | Stanford Profiles
We applied SPD-NGS to two classes of proteases, the intracellular caspases, and the ectodomains of the sheddases, ADAMs 10 and ... 10 (1): 3433 Abstract. Multiple hypothesis testing is an essential component of modern data science. In many settings, in ... 10 Abstract. Aging is associated with complex molecular and cellular processes that are poorly understood. Here we leveraged ... 10 Abstract. Echocardiography uses ultrasound technology to capture high temporal and spatial resolution images of the heart ...
SciELO - Brazil - Annual review of immunology Annual review of immunology
Caspase - wikidoc
Types of caspase proteins. Eleven caspases have so far been identified in humans. There are two types of apoptotic caspases: ... initiator (apical) caspases and effector (executioner) caspases. *Initiator caspases (e.g. CASP2, CASP8, CASP9 and CASP10) ... now known as caspase 1) which at the time was the only known caspase. Following this discovery, the other mammalian caspases, ... caspase-3 and caspase-7 pathway; and by external signals (FAS and TNF), along the caspase 8 pathway. Accessed 25 March 2007. ...
TNFRSF6 | Cancer Genetics Web
The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. ...
Dual Role of Caspase 8 in Adipocyte Apoptosis and Metabolic Inflammation | Diabetes | American Diabetes Association
However, caspase 8, in particular, also has additional nona ... Caspases are cysteine-aspartic proteases that were initially ... Caspase 8 Increases With Obesity and Diabetes. To determine whether caspase 8 could potentially play a role in the setting of ... Disruption of Adipocyte Caspase 8 Improves Glucose Homeostasis. To further study the role of caspase 8 in adipose tissue in ... Caspases are best known as major effectors of apoptosis, or programmed cell death. Caspase 8 is the major effector of the death ...
Association of the Variants CASP8 D302H and CASP10 V410I with Breast and Ovarian Cancer Risk in BRCA1 and BRCA2 Mutation...
Background: The genes caspase-8 (CASP8) and caspase-10 (CASP10) functionally cooperate and play a key role in the initiation of ... CASPASE-8 GENE; INACTIVATING MUTATIONS; COMMON VARIANTS; REDUCED RISK; CELL-CYCLE; APOPTOSIS; SUSCEPTIBILITY; POLYMORPHISMS; ...
Gambogic acid induces apoptosis and inhibits colorectal tumor growth via mitochondrial pathways
... pro-caspase-8 (F); pro-caspase-9 (G); and pro-caspase-3 (H). Data are reported as the mean ± SE of at least three experiments. ... caspase-8 (1:500), cytochrome c (1:500), Apaf-1 (1:500), caspase-9 (1:500), caspase-3 (1:500), GAPDH (1:1000), or β-actin (1: ... caspase-8, cytochrome c, apoptotic protease activating factor (Apaf)-1, caspase-9, caspase-3, GAPDH, and β-actin were obtained ... and autocatalytic processing induces the generation of active caspase-8, leading to the activation of caspase-3. This caspase ...
A Functional Cancer Genomics Screen Identifies a Druggable Synthetic Lethal Interaction between MSH3 and PRKDC | Cancer...
B, protein expression of cleaved caspase-3, cyclin A2, γ-H2AX, and β-actin was assessed in HCC2429 [BRD4-NUT], HCC44 [MSH3mut ... B, protein expression of cleaved caspase-3, cyclin A2, γ-H2AX, and β-actin was assessed in HCC2429 [BRD4-NUT], HCC44 [MSH3mut ... S8C). We observed caspase-3 cleavage (Supplementary Fig. S8C) and the appearance of an annexin-V/PI double-positive population ... 2A, top). In marked contrast, no caspase-3 cleavage could be observed after KU60648 treatment (1 μmol/L, 48 hours) in the MSH3- ...
Molecular Vision: Loss of the cone-enriched caspase-7 does not affect secondary cone death in retinitis pigmentosa
... it was first backcrossed to C57BL/6J for ten generations to generate the rd1_C57BL/6J line [7]. The Casp7-/- was already on a ... Eguchi R, Tone S, Suzuki A, Fujimori Y, Nakano T, Kaji K, Ohta T. Possible involvement of caspase-6 and −7 but not caspase-3 in ... Executioner caspase-3 and caspase-7 are functionally distinct proteases. Proc Natl Acad Sci USA. 2008; 105:12815-9. [PMID: ... Caspase activation and neuroprotection in caspase-3- deficient mice after in vivo cerebral ischemia and in vitro oxygen glucose ...
Improved Resuscitation after Cardiac Arrest in Rats Expressing the Baculovirus Caspase Inhibitor Protein p35 in Central Neurons...
16-18by transgenic overexpression of a dominant negative caspase 1 mutant, 19and by ablation of the caspase 1 20and caspase 11 ... 24,25p35 is an active, site-specific inhibitor of caspases, with a high capacity to inhibit caspase 3. 24p35 is cleaved at the ... In vitro and in vivo inhibition of caspases has been achieved by synthetic caspase inhibitor compounds and by viral proteins ... Given the role of caspases in apoptosis, caspase inhibitors may provide neuronal protection after cardiac arrest. To this end, ...
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Vitamin D in combination cancer treatment
This sensitization may be due to restored apoptotic pathway, as indicated by enhanced cleavage of pro-caspase 10 and PARP and ... Enhanced apoptosis is observed which does not involve caspase 3 activation but the translocation of apoptosis inducing factor ( ... 10. Kilkkinen A, Knekt P, Heliovaara M. et al. Vitamin D status and the risk of lung cancer: a cohort study in Finland. Cancer ... and the cleavage of caspase 3 when used in combination with cisplatin in SCC cells (18). Additional studies support these ...
Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction - GentaurTop
Description: The Homogeneous Caspase-3 Assay Kit is a complete assay system designed to measure Caspase-3 activity for ... Description: The Homogeneous Caspase-7 Assay Kit is a complete assay system designed to measure Caspase-7 activity for ... Description: The Homogeneous Caspase-6 Assay Kit is a complete assay system designed to measure Caspase-6 activity for ... Description: The Homogeneous Caspase-8 Assay Kit is a complete assay system designed to measure Caspase-8 activity for ...
Analysis of Some Phytochemicals as Potential Anticancer Agents Targeting the Receptor Protein Associated With Cancer | Research...
It induces caspase-dependent extrinsic apoptosis in human epidermal growth factor receptor 2 (HER2) through the expression of ... The UA nanoparticles lessened tumor size by focusing on caspases and p53 with downregulation of Bcl-2 and cIAP, starting ... 2015). Induction of caspase dependent extrinsic apoptosis through inhibition of signal transducer and activator of ... 2014). 6-Gingerol induces caspase dependent apoptosis and prevents PMA- induced proliferation in colon cancer cells by ...
Caspase-7
Research proven and tested mouse Caspase-7 antibody. Antiody is guaranteed to work in western blot applications and is reactive ... such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, ... Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; ... Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates ...
Annonaceous acetogenin mimic AA005 induces cancer cell death via apoptosis inducing factor through a caspase-3-independent...
AA005 induces a unique type of cell death in colorectal adenocarcinoma cells, characterized by lack of caspase-3 activation or ... AA005 may trigger the cell death via mediated by AIF through caspase-3 independent pathway. Our work provided new mechanisms ... apoptotic body formation, sensitivity to poly (ADP-ribose) polymerase inhibitor Olaparib (AZD2281) but not pan-caspase ... where cytochrome c forms an apoptosome with Apaf-1 and caspase-9 and triggers the caspase cascade. The most important caspase ...