When the chlorella was microfluidized at the pressure of over 10,000 psi, the disruption of cell surface in chlorella was ... Effect of Chlorella Pulverization by Microfluidization on Lutein Micellization in Vitro and Uptake by Caco-2 Cells. Authors. ... chlorella; lutein; microfluidization; bioavailability; in vitro digestion; micellization; Caco-2 cells. Issue Date. 2011-06. ... Effect of Chlorella Pulverization by Microfluidization on Lutein Micellization in Vitro and Uptake by Caco-2 Cells ...
... the apparent permeability of MC-RR across a differentiated intestinal Caco-2 cell monolayer was investigated. We observed a ... from 2 to 37 × 10−8 cm·s−1). Our results suggested that MC-RR would be poorly absorbed orally. As similar low permeability was ... microcystin-RR Caco-2 cells intestinal permeability cyanotoxin toxicology toxicologic test toxin caco-2 cell pharmacokinetic ... Permeability of the Cyanotoxin Microcystin-RR across a Caco-2 Cells Monolayer. Toxins, 2021, 13 (3), pp.178. ⟨10.3390/ ...
Transepithelial glycylsarcosine transport in intestinal Caco-2 cells mediated by expression of H+-coupled carriers at both ...
K-ATPase expression and activity in two epithelial cell culture lines, Madin Darby canine kidney (MDCK) and Caco-2 cells. RNA ... K-ATPase expression and activity in two epithelial cell culture lines, Madin Darby canine kidney (MDCK) and Caco-2 cells. RNA ... K-ATPase expression and activity in two epithelial cell culture lines, Madin Darby canine kidney (MDCK) and Caco-2 cells. RNA ... K-ATPase expression and activity in two epithelial cell culture lines, Madin Darby canine kidney (MDCK) and Caco-2 cells. RNA ...
Cell culture and selection. Four human colorectal cancer cells of Caco-2, HT-29, LoVo and LS174T were used in this study. All ... Cell cycle analysis. Cells (1 × 106) were harvested, washed with PBS and fixed in 70% ethanol. The fixed cells were washed with ... Cell proliferation assay. Cells (6 × 103/200 μl/well) were seeded in 96-well plates. Viable proliferating cells were detected ... The apoptosis and cell cycle of Caco-2 cells by RNA interference. (A) The apoptosis rate. * Compared with two control groups, P ...
Methods: The uptake rate of vigabatrin was measured in Caco-2 cells at pH 6.0 or 7.4 for 15 min after application of 0.1 - 25.0 ... Results: The uptake rate of vigabatrin in Caco-2 cells was pH-dependent. The uptake of vigabatrin was saturable at pH 6.0 with ... The aim of the project was to identify if transporters are involved in cellular uptake of vigabatrin in Caco-2 cells.. ... The inhibitory effect of selected amino acids and -derivatives on the apical vigabatrin uptake in Caco-2 cells was investigated ...
Caco-2 cells are models of absorptive enterocytes. The net transport of fluid from apical to basolateral surfaces results in ... N2 - Caco-2 cells are models of absorptive enterocytes. The net transport of fluid from apical to basolateral surfaces results ... AB - Caco-2 cells are models of absorptive enterocytes. The net transport of fluid from apical to basolateral surfaces results ... abstract = "Caco-2 cells are models of absorptive enterocytes. The net transport of fluid from apical to basolateral surfaces ...
Three to four areas (700 to 1,000 cells) had been captured for each well. Contaminated cells and total DAPI-stained cells had ... Components AND Strategies Cells and infections. Caco-2 cells (ATCC HTB-37) had been cultured in minimal important moderate (MEM ... Echovirus 7 enters polarized Caco-2 intestinal epithelial cells by a clathrin-mediated. November 28, 2017. palomid529 ... Caco-2 cells transfected with siRNAs or treated with 3-MA had been plated in collagen-coated eight-well holding chamber glides ...
... using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were ... Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line. / Wonfor, Ruth ... Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line. In: Journal of ... Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line. Journal of ...
This work thus demonstrated a possible competition between LGG adhesion to MFGM and to epithelial intestinal cells. This ... Adhesive interactions between milk fat globule membrane and Lactobacillus rhamnosus GG inhibit bacterial attachment to Caco-2 ... the presence of MFGM in the dairy matrix was found to decrease significantly the bacterial attachment ability to Caco-2 TC7 ... competition could negatively impact the adhesion capacity of LGG to intestinal cells in vivo, but requires further ...
... and protective effects of Saccharomyces cerevisiae peptide fractions against H2O2-induced oxidative stress in Caco-2 cells. ...
T1 - The expression of most UDP-glucuronosyltransferases (UGTs) is increased significantly during Caco-2 cell differentiation, ... The expression of most UDP-glucuronosyltransferases (UGTs) is increased significantly during Caco-2 cell differentiation, ... The expression of most UDP-glucuronosyltransferases (UGTs) is increased significantly during Caco-2 cell differentiation, ... title = "The expression of most UDP-glucuronosyltransferases (UGTs) is increased significantly during Caco-2 cell ...
... cells. Confluent C2 cells expressing NHE2 and NHE3 were treated with IFN-γ for 2, 24, and 48 h. Adult rats were injected with ... IFN-γ downregulates expression of Na+/H+ exchangers NHE2 and NHE3 in rat intestine and human Caco-2/bbe cells. / Rocha, Flavio ... cells. Confluent C2 cells expressing NHE2 and NHE3 were treated with IFN-γ for 2, 24, and 48 h. Adult rats were injected with ... cells. Confluent C2 cells expressing NHE2 and NHE3 were treated with IFN-γ for 2, 24, and 48 h. Adult rats were injected with ...
Return to Article Details Protective Effects of Punicalagin on Caco-2 Intestine Cell Line under Oxidative Stress Caused by Tert ...
The Caco-2 cell monolayer model is a popular surrogate in predicting the in vitro human intestinal permeability of a drug due ... N2 - The Caco-2 cell monolayer model is a popular surrogate in predicting the in vitro human intestinal permeability of a drug ... AB - The Caco-2 cell monolayer model is a popular surrogate in predicting the in vitro human intestinal permeability of a drug ... abstract = "The Caco-2 cell monolayer model is a popular surrogate in predicting the in vitro human intestinal permeability of ...
Caco-2. Human. Colon. Colorectal carcinoma. ECACC Cellosaurus Cal-27. Human. Tongue. Squamous cell carcinoma. ATCC Cellosaurus ... These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a ... Accumulation of apoptotic/necrotic (dead) cells. *Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop ... Cell strains edit A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells ...
Cell Culture.. Caco-2 cells were obtained from the American Type Culture Collection (Manassas, VA) and grown in Dulbeccos ... LLC-PK1 cells (porcine kidney epithelial cell line) and LLC-GA5-COL300 cells (a transformant cell line derived by transfecting ... 105 for LLC-PK1 cells and 3.125 × 105 for LLC-GA5-COL300 cells. The cells were grown for 3 days, and the culture medium was ... vinblastine uptake by LLC-PK1 cells and LLC-GA5-COL300 cells. In LLC-PK1 cells, the uptake of [3H]vinblastine at steady state ...
... analysed by ICP-MS in a long-term Caco-2 TC7 cell model. Download Prime PubMed App to iPhone, iPad, or Android ... Furthermore, this accumulation was more marked in cells exposed long-term to cadmium compared with controls, and that this ... Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 micromol Cd/l for 5 ... Results showed that Caco-2 TC7 cells incubated long-term with cadmium concentrations ranging from 0 to 10 micromol Cd/l for 5 ...
Ablation of DNMT3A in Caco-2 cells results in global DNA hypomethylation, which is linked to impaired regenerative capacity, ... Genetic deletion of Dnmt3a in intestinal epithelial cells (Dnmt3aΔIEC) in mice confirms the phenotype of an altered epithelial ... DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in ... ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in ...
The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight ... The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight ... the probiotic formula was well tolerated and FGID severity was significantly reduced after 14 days of treatment with the 2 ... revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells ...
Topology analysis identified a set of proteins directly linked to the SAR-CoV2 titer, including ACE2, a SARS-CoV-2 binding ... The current study employed Bayesian networks to a longitudinal proteomic dataset generated from Caco-2 cells transfected with ... SARS-CoV-2 (isolated from patients returning from Wuhan to Frankfurt). Two different approaches were employed to assess the ... Cancer; Colorectal tumor; Non-small-cell lung cancer; Ovary tumor; Small-cell lung cancer; Soft tissue sarcoma; Transitional ...
Caco-2 Cells. Wang R, Löhr CV, Fischer K, W Dashwood M, Greenwood JA, Ho E, Williams DE, Ashktorab H, Dashwood MR, Dashwood RH ... HT29 Cells. Wang R, Löhr CV, Fischer K, W Dashwood M, Greenwood JA, Ho E, Williams DE, Ashktorab H, Dashwood MR, Dashwood RH. ... Cell Movement. Wang R, Löhr CV, Fischer K, W Dashwood M, Greenwood JA, Ho E, Williams DE, Ashktorab H, Dashwood MR, Dashwood RH ... HCT116 Cells. Wang R, Löhr CV, Fischer K, W Dashwood M, Greenwood JA, Ho E, Williams DE, Ashktorab H, Dashwood MR, Dashwood RH ...
Fatty acids increase paracellular absorption of aluminium across Caco-2 cell monolayers. Chem Biol Interact. 2009 Oct 7. 181(2 ... Aluminum-induced defective mitochondrial metabolism perturbs cytoskeletal dynamics in human astrocytoma cells. J Neurosci Res. ... In vivo genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and ... As a result, fully vaccinated children are exposed to up to 4 mg of aluminum in the first 2 years of life. [25] Mitkus et al ...
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug ... the use of highly-characterized human iPS cell-derived colon organoids as an alternative cell model for traditional Caco-2 cell ... Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid ... 3D Cell Culture. Using Human iPS Cell-derived Colon Organoids for Cytotoxicity Screening of Drug Compounds ...
Cadmium affects autophagy in the human intestinal cells Caco-2 through ROS-mediated ERK activation. October 6, 2021. by Jose ... Tags bsa standard, bsep antibody, buccal swab procedure, cdna synthesis kit, cell counter machine, cgmp elisa, cloning pcr ... mtt cell proliferation assay, nmnat1 antibody, olig2 antibody, Opossum, Orangutan, otx2 antibody, probe, protein carbonyl assay ... Now we have beforehand proven that Cd induces ERK1/2 activation in differentiated however … Read More ...
In human Caco-2 colon epithelial cells as well as the lung cell line A549 stably expressing ACE2 and TMPRSS2, quinine also ... In Vero cells, quinine inhibited SARS-CoV-2 infection more effectively than CQN, and H-CQN and was less toxic. ... In consistence with Vero cells, quinine was less toxic in A549 as compared to CQN and H-CQN. Finally, we confirmed our findings ... dependent on the cell line and multiplicity of infection (MOI). Conclusively, our data indicate that quinine could have the ...
Title: HISTIDINE CONTENT OF LOW MOLECULAR WEIGHT BEEF PROTEINS INFLUENCES NONHEME IRON BIOAVAILABILITY IN CACO-2 CELLS Author. ... Nonheme eiron bioavailability was assessed by Caco-2 cell monolayer 59Fe uptake using an extrinsic labeling method. All ... HISTIDINE CONTENT OF LOW MOLECULAR WEIGHT BEEF PROTEINS INFLUENCES NONHEME IRON BIOAVAILABILITY IN CACO-2 CELLS. JOURNAL OF ... Publication Date: 2/1/2002 Citation: SWAIN, J.H., TABATABAI, L.B., REDDY, M.B. ...
... cells, in a dose-dependent manner. Adenine induced significant apoptosis in HT29 cells, whereas Caco-2cells ... i,Results,/i,. Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2  ... signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. ,i,Conclusions,/i,. Our findings ... i,Methods,/i,. Cell viability was measured using the MTT assay. Levels of phosphorylation and protein expression were ...
Poor correlation between Caco-2 cells and artificial membrane (PAMPA) assays Note axis scales Balimane et al., AAPS J 8, e1-e13 ... Visualise Models Using Cell Designer JC_C-0.07-1_Measurement JC_N-0.07-1_Measurement ... Poor relationship between Caco-2 permeability and log Ko/w r2 = 0.097 THESE THEORIES OF DRUG UPTAKE WERE BIOPHYSICAL, LIPID- ... The human metabolic network (2) • Not yet compartmentalised • 2,823 reactions (incl 300 orphans), of which 2,215 have disease ...
d-f) The relative number of cells represents the ratio of cells near the agar drop at 5 min to that at 0 min. Values are ... Szymanski, C. M., King, M., Haardt, M. & Armstrong, G. D. Campylobacter jejuni motility and invasion of Caco-2 cells. Infect. ... b) The relative number of cells represents the ratio of cells near the agar drop at 5 min to that at 0 min. Values are ... b) The relative number of cells represents the ratio of cells near the agar drop at 5 min to that at 0 min. Values are ...