Mesh term Branched DNA Signal Amplification Assay. Browse to parent terms:. Molecular Probe Techniques. Nucleic Acid ... A molecular probe technique that utilizes branched DNA (bDNA) as a means to amplify the hybridization signal. One end of the ... while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal ...
Branched DNA Signal Amplification Assay.. Reagents required for RNA analysis (i.e., lysis buffer, amplifier/label probe buffer ... were analyzed for mRNA expression of each gene by branched DNA signal amplification assay. The data are represented as the ... were analyzed for mRNA expression of each gene by branched DNA signal amplification assay. Because Asbt, IBABP, and Fgf15 are ... were analyzed for mRNA expression of each transporter by branched DNA signal amplification assay. The data are represented as ...
Branched DNA Signal Amplification Assay.. The mRNA expression of mouse Kim-1 (kidney injury molecule-1), PCNA (proliferating ... were quantified using the branched DNA 1.0 signal amplification assay (Affymetrix, Inc.) (Hartley and Klaassen, 2000). Novel ... differential expression of rat hepatic cytochrome P450 mRNA transcripts using branched DNA signal amplification technology. ... Transcription Factor Binding Assays.. Nuclear extracts were used to quantify DNA binding of Nrf2 and the p65 subunit of nuclear ...
... this invention enables the extraction of circulating RNA from plasma or serum and utilizes nucleic acid amplification assays ... using nucleic acid amplification assays, such as, but not limited to, polymerase chain reaction (RT-PCR), branched DNA signal ... branched DNA signal amplification, transciption-based amplification, amplifiable RNA reporters, boomerang DNA amplification, ... branched DNA signal amplification, and self-sustained sequence replication assays, such as isothermal nucleic acid sequence ...
Branched DNA Assay. In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification ... The assay can be used to detect and quantify many types of RNA or DNA target. In the assay, branched DNA is mixed with a sample ... Famous quotes containing the words assay, branched and/or dna:. "The lyf so short, the craft so longe to lerne,. Th assay so ... Several different short single-stranded DNA molecules (oligonucleotides) are used in a branched DNA-assay. The capture and ...
Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay. J Acquir Immune Defic ...
... is a ubiquitous DNA virus that infects the majority of the adult population. In the immunocompetent host, infection is ... Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched-DNA signal amplification assay. J Clin ... Comparison of three assays for cytomegalovirus detection in AIDS patients at risk for retinitis. J Clin Microbiol. 2000 Feb. 38 ... Quantitative cytomegalovirus DNA level in the blood and its relationship to cytomegalovirus retinitis in patients with acquired ...
... is a ubiquitous DNA virus that infects the majority of the adult population. In the immunocompetent host, infection is ... Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched-DNA signal amplification assay. J Clin ... Comparison of three assays for cytomegalovirus detection in AIDS patients at risk for retinitis. J Clin Microbiol. 2000 Feb. 38 ... Cytomegalovirus (CMV) blood DNA load, CMV retinitis progression, and occurrence of resistant CMV in patients with CMV retinitis ...
Quantitation of HBV DNA in human serum using branched DNA signal amplification assay.Am. J. Clin. Pathol.1041995537546. ... bDNA hybridization assay and serology.HBV DNA in serum was quantitated by the signal amplification method with enzymatically ... The presence of HBV DNA in these samples was then assessed by RTD-PCR and bDNA signal amplification assays. ... the assay has been replaced by a simpler and more accurate HBV DNA quantification system that uses a branched-DNA (bDNA) probe ...
In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to ... "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml". Nucleic Acids ... amplifier The assay can be used to detect and quantify many types of RNA or DNA target. In the assay, branched DNA is mixed ... Several different short single-stranded DNA molecules (oligonucleotides) are used in a branched DNA-assay. The capture and ...
Urdea, M. S. Branched DNA signal amplification. Does bDNA represent post-PCR amplification technology? Biotechnology 12:926-927 ... Carlowicz, M. Rapid tuberculosis assay under expedited FDA review. Clin. Lab. News July 1-4, 1995.Google Scholar ... Wilber, J. C. Direct quantification of viral nucleic acids using branched DNA (bDNA) signal amplification: methodology and ... Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Science 239:487-491, 1988.PubMedCrossRef ...
... simultaneous branched DNA (bDNA) signal amplification and background suppression. ... DNA and RNA assays, and all other relevant molecular techniques using DNA and RNA. When circulating tumor cells are captured ... Each fully assembled amplification structure is contained within 40−50 bp of target RNA with the capacity for 400-fold signal ... An oligo pair hybridization event is essential for support of the signal amplification structure, which is assembled by a ...
Signal-based amplification such as oligonucleotide ligation assay (OLA), Q.β. RNA replicase (Lizardi and Kramer. 1991. TIB 9: ... Biotechniques 9: 142-147) and branched DNA (bDNA) (Urdea. 1993. Clin. Chem. 39: 725-726), amplify or alter a signal from a ... Direct linear amplification thermal cycle sequencing34,35 of double stranded PCR products was accomplished using exo− Pfu DNA ... DNA Amplification. Total cellular DNAs were on hand in our laboratory stocks. These had been purified by conventional methods ...
... probe amplification using ligase chain reaction or Q-beta replicase; and (3) signal amplification, as in branched DNA assay.1 ... Conventional PCR is designed to result in the amplification of a specific DNA fragment and subsequent analysis is required to ... transcription mediated amplification, nucleic acid sequence based amplification (NASBA), and so on; (2) ... Mutations in DNA sequences can also be detected by real time PCR, as the stability of the probe-target duplex can be measured. ...
1997) A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml. Nucleic ... 2006) A multiplex branched DNA assay for parallel quantitative gene expression profiling. Anal. Biochem. 352, 50-60. ... This method is hybridization-based and incorporates branched DNA (bDNA) technology (44, 45). Because it relies on signal ... Multiplex Assays for Measuring Cytokine Protein Level. Multiplex assays for 66 mouse cytokines (covering most of the well- ...
Pachl et al., 1995, "Rapid and Precise Quantification of HIV-1 RNA in Plasma Using a Branched DNA Signal Amplification Assay", ... Pachl et al., 1995, Rapid and Precise Quantification of HIV 1 RNA in Plasma Using a Branched DNA Signal Amplification Assay , J ... 5.1.12 QUALITATIVE DNA PCR. Qualitative DNA PCR was performed on lyzed cells without purification of DNA. Amplification was ... viral DNA synthesis was measured by qualitative DNA PCR following infection. No significant HIV-1 DNA PCR signal was observed ...
Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay. AIDS 7(Suppl. 2 ... The Quantiplex HIV RNA assay (Chiron)-based on branched DNA (44), the HIV-1 RNA QT (Organon Teknika) NASBA (nucleic acid ... 1993) Genetic relationships determined by a DNA heteroduplex mobility assay: analysis of HIV-1 env genes. Science 262:1257-1261 ... 1996) COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR. Clin. Chem. 42 ...
Quantitative assays for the measurement of SIV RNA were performed by using a branched chain DNA signal amplification assay ... Because the incorporation of BrdUrd into cellular DNA occurs via the nucleotide salvage pathway, which is preferentially used ... BrdUrd is incorporated into the DNA of dividing cells, is not reused after incorporation, and can be detected by flow ... specific for SIV (18). The lower quantitation limit of this assay is 10,000 copies of SIV RNA per milliliter of plasma. ...
... which was measured as the concentration of HIV-1 RNA found using a sensitive branched-DNA signal-amplification assay. ...
Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay. AIDS 1994; 7 ( ... Mulder J, McKinney N, Christopherson C, Sninsky J, Greenfield L, Kwok S. Rapid and simple PCR assay for quantitation of human ... Discordance of RT sequences conferring ZDV resistance in proviral DNA from brain and spleen. HIV Drug Resistance Third ... resistant human immunodeficiency virus isolates to antiviral agents determined by using a quantitative plaque reduction assay. ...
... nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, ... extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA ... Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, ... malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans ...
The QuantiGene assay uses branched DNA (bDNA) technology, relying on signal amplification instead of target amplification for ... Plus: 6 tips for QuantiGene assays When you want an assay that can detect nucleic acids, youll likely choose the high-quality ... xMAP®-powered Assay Provides Critical Clues from Liquid Biopsy Samples. Posted on March 5, 2019. (February 14, 2019) by Hilary ... 5 Tips for Multiplex Kit Users and Assay Developers. Posted on November 21, 2017. (February 12, 2019) by Hilary Graham ...
These characterized transcripts were then quantified using the bDNA assay. Comparisons were made using a ratio of signal per ... signal amplification technology. For example, HIV RNA is detected in a plasma sample by hybridization of multiple specific ... Branched DNA for quantification of viral load.. Wilber JC1.. Author information. 1. Chiron Corporation, Emeryville, California ... acids in patient samples can be quantified directly using a solid phase nucleic acid hybridization assay based on branched DNA ...
Serum HCV-RNA was quantitatively measured by a branched DNA signal amplification assay (Versant HCV-RNA 3.0 Assay bDNA; Bayer, ... The sensitivity of the assay was 50 HCV-RNA copies/ml. HCV was genotyped by a hybridisation assay (Inno-LIPA; Innogenetics, ... Ravaggi A, Zonaro A, Marin MG, et al. Distribution of viral genotypes in Italy determined by hepatitis C virus typing by DNA ... Antibodies to nuclear, smooth muscle, mitochondrial, and liver and kidney microsomal antigens were assayed on rat liver and ...