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*  Isolation and culture of inner cell mass cells from human blastocysts | ScholarBank@NUS
Bongso, A., Fong, C.-Y., Ng, S.-C., Ratnam, S. (1994). Isolation and culture of inner cell mass cells from human blastocysts. ... Totipotent non-committed inner cell mass (ICM) cells from human blastocysts, if demonstrated to be capable of proliferating in ... All 21 embryos were grown from the 2-pronuclear until blastocyst stages on a human tubal epithelial monolayer in commercial ... The medium was changed after blastocyst formation to Chang's medium supplemented with 1000 units/ml of human leukaemia ...
http://scholarbank.nus.edu.sg/handle/10635/131314
*  Zavos - Congress 2001
The only advantage of PGD is that it allows SCNT blastocysts, which have a greater than 50% probability of being ... This (it is believed) is one of the reasons for only (on average) 50% of the NT blastocysts implanting [7]. ... The advantage of PGD is that it provides a rapid means for diagnosing which morphologically normal blastocysts are actually ... Although technically a morula, this ball is ubiquitously referred to as a blastocyst in the scientific literature, and will ...
http://zavos.org/library/library_congress01.htm
*  Cryopreservation | Irvine Scientific
Irvine Scientific provides cryopreservation media for conventional slow-freezing and thawing of cleavage and blastocyst stage ...
http://www.irvinesci.com/assisted-reproductive-technology/art-processes-and-protocols/cryopreservation
*  Shawn P. Murphy, Ph.D. - University of Rochester Medical Center
Trophoblast cells are the only cells derived from the blastocyst that are in direct contact with maternal blood, and therefore ...
https://www.urmc.rochester.edu/people/26673547-shawn-p-murphy
*  Parthenogenetic development of Mos-deficient mouse oocytes." by Y Hirao and J J. Eppig
... oocytes in vitro may adversely affect preimplantation development and reduce the frequency of blastocyst formation even under ... In total, 5% of the Mos-/- oocytes developed to the blastocyst stage. Preimplantation-like and early postimplantation-like ... Blastocyst: cy, ul, Cell-Division, Female, Metaphase, Mice, Oocytes: gd, cy, ul, Phenotype, Photogrammetry, Proto-Oncogene- ... In total, 5% of the Mos-/- oocytes developed to the blastocyst stage. Preimplantation-like and early postimplantation-like ...
https://mouseion.jax.org/stfb1990_1999/936/
*  Finding biomarkers in non-model species: literature mining of transcription factors involved in bovine embryo development |...
A characteristic feature of blastocyst development in ruminants (cattle, sheep) and in pig is the elongation process (Figure 1 ... Chang MC: Development of bovine blastocyst with a note on implantation. Anat Rec. 1952, 113: 143-161. 10.1002/ar.1091130203. ... This approach was applied to identify novel transcription factors during bovine blastocyst elongation, a process that is not ... However, molecular-genetic data on ruminant embryo development are scant and blastocyst elongation is not observed in rodents ...
https://biodatamining.biomedcentral.com/articles/10.1186/1756-0381-5-12
*  Array comparative genomic hybridization screening in IVF significantly reduces number of embryos available for cryopreservation...
All group B patients (48/48) had at least one blastocyst remaining for cryopreservation. A total of 157 (40.4%) blastocysts ... Incorporation of aCGH screening significantly reduced the total number of cryopreserved blastocysts compared to when ... 389 good morphology blastocysts were identified and a single top quality blastocyst was selected for fresh transfer. ... All group B patients (48/48) had at least one blastocyst remaining for cryopreservation. A total of 157 (40.4%) blastocysts ...
https://www.csm.ox.ac.uk/publications/348904
*  Cumulus-specific genes are transcriptionally silent following somatic cell nuclear transfer in a mouse model. | ScholarBank@NUS
Expression of cumulus-specific genes in SCNT-derived embryos at 2-cell, 4-cell and day 4.5 blastocyst stages was compared with ... followed by activation in 10 micromol/L strontium chloride for 5 h and subsequent in vitro culture up to the blastocyst stage. ... which continually remained silent at the 4-cell and blastocyst stages. It is therefore concluded that all four cumulus-specific ...
http://scholarbank.nus.edu.sg/handle/10635/46585
*  Reproductive Immunology Forum: Recurrent implantation failure with PGD tested embryos from donor
... only one of them resulted in a transfer of a 5 day embryo that almost became a blastocyst. I was diagnosed as a poor responder ...
http://preventmiscarriage.com/recurrent-implantation-failure-with-pgd-tested-e.html
*  Blastocyst Implantation |authorSTREAM
HATCHING OF THE BLASTOCYST : HATCHING OF THE BLASTOCYST Formation of blastocyst (D4) Shedding of zona pellucida & hatching of ... Implantation of Blastocyst Implantation of Blastocyst : Implantation of Blastocyst Day - 4: Morula reaches in uterine cavity ... SEQUENCE AFTER FERTILIZATION? 1st cleavage after 30 Hours Morula 3days Blastocyst formation 4th day Blastocyst floats in Uterus ... Blastocyst Implantation Prof. Dr. Saeed Shafi Slide 2: Nadia Suleyman, a 32-year old business executive underwent IVF and ...
http://www.authorstream.com/Presentation/msaeedshafi-157600-blastocyst-implantation-entertainment-ppt-powerpoint/
*  Blastocyst Images, Stock Photos & Vectors | Shutterstock
Find blastocyst Stock Images in HD and millions of other royalty-free stock photos, illustrations, and vectors in the ... Blastocyst stock photos. 850 Blastocyst stock photos, vectors, and illustrations are available royalty-free. See blastocyst ... blastocyst. Human blastocyst, with inner cell mass. the mammalian conceptus in the post-morula stage, consisting of the ... blastocyst. Human blastocyst, with inner cell mass. the mammalian conceptus in the post-morula stage, consisting of the ...
https://www.shutterstock.com/search/blastocyst
*  OCT4/POU5F1 is required for NANOG expression in bovine blastocysts | PNAS
This mimics findings in OCT4-deficient human blastocysts but is in sharp contrast to Oct4-null mouse blastocysts, where NANOG ... OCT4/POU5F1 is required for NANOG expression in bovine blastocysts. Kilian Simmet, Valeri Zakhartchenko, Julia Philippou- ... OCT4/POU5F1 is required for NANOG expression in bovine blastocysts. Kilian Simmet, Valeri Zakhartchenko, Julia Philippou- ... Control blastocysts showed a typical salt-and-pepper distribution of NANOG- and GATA6-positive cells in the ICM. In contrast, ...
http://www.pnas.org/content/115/11/2770.short?rss%3D1
*  Blastocyst - definition of blastocyst by The Free Dictionary
blastocyst synonyms, blastocyst pronunciation, blastocyst translation, English dictionary definition of blastocyst. n. The ... blastocyst. Also found in: Thesaurus, Medical, Encyclopedia, Wikipedia.. Related to blastocyst: blastocyst transfer ... However at the ARU, blastocyst culture enables embryos to be "grown" in a lab to the blastocyst stage of development (day five ... The number of embryos or blastocysts transferred in each cycle was captured, but embryo and blastocyst transfers were not ...
https://www.thefreedictionary.com/blastocyst
*  Implantation of Blastocysts & IVF Embryos in Humans
Implantation of blastocyst embryos in humans is complex. Hormones and other factors, both embryonic & uterine influence ... Human blastocysts should hatch from the shell and begin to implant 1-2 days after day 5 IVF blastocyst transfer. In a natural ... Picture of a human blastocyst Inner cell mass - ICM Trophectoderm - TE More blastocyst pictures and grading issues ... Ovulation - mating - fertilization - and subsequent development to the blastocyst stage occurs. The blastocyst then remains in ...
https://www.advancedfertility.com/implantation.htm
*  JCI - Making the blastocyst: lessons from the mouse
Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril. 2000;73(6): ... Making the blastocyst: lessons from the mouse Katie Cockburn et al. * Portrait of an oocyte: our obscure origin Roger Gosden et ... while Oct4 expression becomes limited to the ICM in the early blastocyst stage. By the late blastocyst stage, while continuing ... Making the blastocyst: lessons from the mouse. Katie Cockburn and Janet Rossant Department of Molecular Genetics, University of ...
https://www.jci.org/articles/view/41229
*  JCI - Making the blastocyst: lessons from the mouse
expression becomes limited to the ICM in the early blastocyst stage. By the late blastocyst stage, while continuing to express ... are not established until the late blastocyst stage. The expression patterns of NANOG. and GATA6. in the human preimplantation ... and recent discoveries have shed new light on the establishment of the three blastocyst lineages. What is less clear, however, ... results in the formation of a blastocyst with three distinct cell lineages. Only one of these lineages, the epiblast, ...
https://www.jci.org/articles/view/41229/figure/1
*  Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts | PNAS
... and found that all of them were able to produce NT blastocysts at efficiencies ranging from 10 to 20%, a lower blastocyst- ... Individual whole blastocysts or isolated ICMs were placed in separate wells of a 12-well dish seeded with a monolayer of gamma- ... Whole blastocysts and fibroblasts were used as controls. Total RNA was isolated using the Qiagen RNeasy Mini Kit and then was ... After 48 h, blastocysts/ICMs that failed to adhere to the feeder layer were physically pressed against the bottom of the ...
https://www.pnas.org/content/115/9/2090
*  Blastocyst Images | DR-MALPANI
For example, a blastocyst quality grade of 4AB means that the blastocyst is expanded (grade 4), has many tightly packed cells ... This blastocyst grading system assigns 3 separate quality scores to each blastocyst embryo: ... Blastocyst Hatching out of Zona (shell) = Grade 5. (I) Inner Cell mass = Grade A (Very Good). (T) Trophectoderm = Grade A (Very ... Blastocyst completely hatched = Grade 6. (I) Inner Cell mass = Grade A (Very Good). (T) Trophectoderm = Grade A (Very Good) ...
https://www.drmalpani.com/blogs/blastocystimages
*  The Morula and Blastocyst
The cells inside the blastocyst are called the inner cell mass and give rise to the head, body, and other structures vital to ... By 4 to 5 days, a cavity forms within this ball of cells and the embryo is then called a blastocyst. ... The Morula and Blastocyst. Select Subtitle Language. 88 languages available. Afrikaans. Albanian (Tosk) [Shqip]. Arabic [عربي] ...
https://www.ehd.org/movies.php?mov_id=6
*  What is a Blastocyst? (with pictures)
A blastocyst is a cellular mass that forms early in the embryo development process in mammals. Humans develop a blastocyst ... So a blastocyst is very early pregnancy? Is a blastocyst before implantation? It sounds like it would have to be if part of the ... Apparently, a blastocyst isn't an embryo yet. (In humans, the term embryo only applies after implantation.) At the blastocyst ... Within the blastocyst, there are two types of cells. In the interior is the inner cell mass, a portion of which will begin to ...
http://www.wisegeek.org/what-is-a-blastocyst.htm
*  Blastocyst Injection - Genetically Engineered Mouse Facility | MD Anderson Cancer Center
Blastocyst Injection. The ability of mouse pre-implantation embryos to incorporate cells from other embryos is fundamental to ... Genetically modified ES cells will be injected into C57Bl/6 or C57Bl/6 albino blastocysts. Injected blasts will then be ... ES cell clone contributing to the germ-line is highly dependent on the quality of the ES cells injected into the blastocyst. It ...
https://www.mdanderson.org/research/research-resources/core-facilities/genetically-engineered-mouse-facility/services-and-fees/blastocyst-injection.html
*  Poor quality blastocyst - Fertility Treatments | Forums | What to Expect
I have just had a grade 5CC blastocyst and an early blastocyst transferred yesterday. My egg quality has been an issue for me, ... I have just had a grade 5CC blastocyst and an early blastocyst transferred yesterday. My egg quality has been an issue for me, ... I feel like my blastocyst is a lost cause, but then I hear stories like yours and it gives me a glimmer of hope. I hope you ...
https://www.whattoexpect.com/forums/fertility-treatments/topic/poor-quality-blastocyst.html
*  Blastocyst Transfer | Community Health Network
Advantages of Blastocyst Transfer. *Transferring embryos that have developed to blastocyst stage should help improve pregnancy ... The blastocyst stage is the point at which the embryo normally exists within the uterus and is usually capable of implantation ... With blastocyst transfer, fewer embryos are transferred to the uterus, which limits the possibility of high-order multiple ... Generally, with blastocyst transfer excellent pregnancy rates can be achieved by transferring only two embryos. Our most recent ...
https://www.ecommunity.com/services/fertility-care/in-vitro-fertilization-ivf/blastocyst-transfer
*  Generation of ES Cells from Blastocysts - Genetically Engineered Mouse Facility | MD Anderson Cancer Center
Genetically Engineered Mouse Facility at MD Anderson offers many services including the generation of ES Cells from blastocysts ... Generation of ES Cells from Blastocysts. The GEMF is now able to generate novel ES cell lines from blastocysts. This technique ... Blastocysts will be isolated and grown to obtain the inner cell mass. The ICM will then be dissociated to obtain ES cells. ES ... Day 12: Blastocyst outgrowth is checked for the presence of a distinct inner cell mass (ICM). If a defined ICM is detected, it ...
https://www.mdanderson.org/research/research-resources/core-facilities/genetically-engineered-mouse-facility/services-and-fees/es-cell-generation.html