Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid...
... in congenic recipient mice was assessed 16 hours after intraperitoneal injection of 1 mg/animal BrdU. As a control, B cells ( ... Animals were housed in a specific pathogen-free facility at the Max-Delbrück-Center for Molecular Medicine, Berlin. All animal ... Eμ-Myc (C57BL/6) transgenic mice,16 C57BL/6 Ly5.1 (CD45.1) congenic mice, LTα−/− mice,17 and Rag2−/− mice were obtained from ... C57BL/6 Ly5.1 (CD45.1) congenic, LTα−/−, and Rag−/− mice 7 to 12 weeks of age were injected intravenously with 1 × 104 to 2 × ...http://www.bloodjournal.org/content/118/4/1020?sso-checked=true
2nd Congress of the Slovenian Society for Laboratory Animals, Ljubljana 2014 - VideoLectures - VideoLectures.NET
Protein digestibility and bioavailability of the F2 homozygous crossing line of the congenic mice for the lean locus Fob3b2, ... Micro and macro environment in rodents animal facilitiesMicro and macro environment in rodents animal facilities. ... Helping the public to understand animal researchHelping the public to understand animal research. ... Event: Conferences » Other » 2nd Congress of the Slovenian Society for Laboratory Animals, Ljubljana 2014 2nd Congress of the ...http://videolectures.net/slolasa2014_ljubljana/
Transgene-specific CD4+ T-cell responses using Mir142T- | Open-i
The levels of transgene-specific T cells in the animal can be tracked over time following the injection of congenic (CD45.1+) ... The levels of transgene-specific T cells in the animal can be tracked over time following the injection of congenic (CD45.1+) ... whereas additional and protracted antigenic presentation occurred in dystrophic animals, leading to secondary CD4+ T-cell ... whereas additional and protracted antigenic presentation occurred in dystrophic animals, leading to secondary CD4+ T-cell ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4283385_gt201461f5&req=4
Age-Dependent Changes in FasL (CD95L) Modulate Macrophage Function in a Model of Age-Related Macular Degeneration | IOVS | ARVO...
32 The conditional knockout line was verified as congenic with the C57BL/6J strain by microsatellite analysis (Research Animal ... All animal experiments were approved by the Animal Studies Committee at Washington University School of Medicine, and conform ... many animal studies designed to explore the mechanisms of AMD are performed on relatively young animals, 6-11 which may not ... and maintaining them in our animal facility until the desired age. Young animals typically were 6 to 12 weeks, while aged mice ...http://iovs.arvojournals.org/article.aspx?articleid=2128013
JCI Insight - Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage
Animals. For the study of collagen antibody-induced arthritis, the B10.Q mouse strain was used, while a Cia9i congenic mouse ... The animal experiments were approved by the Stockholm Ethical Committee (N490/12, H2014-483) and the national Animal Experiment ... Cia9i congenic strain is derived from B10.Q-NOD Cia9 congenic mice as previously described (40). All mice were kept and bred in ... in the animal house of the Division of Medical Inflammation Research, Karolinska Institutet and the Central Animal Laboratory ...https://insight.jci.org/articles/view/93688
Lymphocyte Activation Gene-3 (CD223) Regulates the Size of the Expanding T Cell Population Following Antigen Activation In Vivo...
Thy1.1 congenic) were obtained from The Jackson Laboratory (Bar Harbor, ME). All animal experiments were performed in American ... Animal protocols were approved by the St. Jude and the Trudeau Institute Institutional Animal Care and Use Committees. ... Association for the Accreditation of Laboratory Animal Care-accredited, specific pathogen-free facilities following national, ...http://www.jimmunol.org/content/172/9/5450?ijkey=ae7d33b4dc81cd6401fb5f48497b07896949a16c&keytype2=tf_ipsecsha
Frontiers | Plxnd1 Expression in Thymocytes Regulates Their Intrathymic Migration While That in Thymic Endothelium Impacts...
Here we use Plxnd1flox/flox mice crossed to pLck-Cre, pKeratin14-Cre or pTek-Cre transgenic animals to create cell-type ... Here we use Plxnd1flox/flox mice crossed to pLck-Cre, pKeratin14-Cre or pTek-Cre transgenic animals to create cell-type ... progenitors to repopulate Ly5 congenic animals post-transplant (15). Cortex and medulla were identified using anti-CD4 and anti ... All the animal experiments were performed under animal protocols approved by Dana-Farber Cancer Institute and Harvard Medical ...https://www.frontiersin.org/articles/10.3389/fimmu.2013.00392/full
US20020082409A1 - Stresscopins and their uses - Google Patents
... tissues or organs can be maintained as allogeneic or congenic grafts or transplants, or in culture. The transgenic animals may ... A non-human transgenic animal model for stresscopin gene function wherein said transgenic animal comprises an introduced ... be any non-human mammal, such as laboratory animals, domestic animals, etc. The transgenic animals may be used in functional ... body weight and food intake were reduced in stresscopin-treated animals as compared to control mice receiving saline vehicle. ...https://patents.google.com/patent/US20020082409A1/en
Hematopoietic stem and progenitor cells are differentially mobilized depending on the duration of Flt3-ligand administration |...
C57BL/6-Ly5.1 congenic recipients were bred in our own facilities and were used at 6-12 weeks of age. The animals were fed ... Animals. Male Balb/c and C57BL/6 mice (8-12 weeks old) were purchased from Charles River (Maastricht, the Netherlands). ... This was in sharp contrast with the animals in the group given PBMC that were harvested following FL administration for 10 days ... Background Flt3-ligand is a cytokine that induces relatively slow mobilization of hematopoietic cells in animals and humans in ...http://www.haematologica.org/content/95/7/1061?ijkey=ffede44264dcc7536656a404b5f4f6ada7141a15&keytype2=tf_ipsecsha
Hypertension Suppression, Not a Cumulative Thrust of Quantitative Trait Loci, Predisposes Blood Pressure Homeostasis to...
Generation of Backcross Animals. The process was similar to that of creating a congenic strain,5 except that no chromosome ... Animals. Protocols for handling as well as maintaining animals were approved by our institutional animal committee (CIPA). The ... Construction of New Congenic Strains. Congenic strains bear different segments of Lewis chromosomes that are replaced by the ... Manufacture of Congenic Combinations. A congenic strain for 1 chromosome was coalesced with another from a separate chromosome ...http://circgenetics.ahajournals.org/content/8/4/610
Topological analysis of metabolic networks integrating co-segregating transcriptomes and metabolomes in type 2 diabetic rat...
We subsequently used the congenic panel to map quantitative trait loci underlying specific mQTLs and genome-wide eQTLs. ... reflecting the metabolic consequences of series of linked genetic polymorphisms in the congenic intervals. ... tissue to map molecular phenotypes to genomic blocks associated with obesity and insulin secretion in a series of rat congenic ... Animals. A colony of GK/Ox rats bred locally and derived in 1995 from the GK/Par colony was used to produce the congenic ...https://0-genomemedicine-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/s13073-016-0352-6
Metabolic Studies Using Rat as Model Organism sub-cluster 89
Animal husbandry staff (group 1) and researchers (group 2) were recruited from a University animal facility together with ... which arose through a spontaneous mutation within the MHC-congenic background strain LEW.1AR1. The LEW.1AR1-iddm rat is ... Identification of animal strain differences in risk for binge eating could highlight genetic differences across individuals of ... Mice are increasingly being used in behavioral neuroscience, largely replacing rats as the behaviorist's animal of choice. ...http://www.biomedsearch.com/cluster/0/Metabolic-Studies-Using-Rat-as-Model-Organism/sub-89.html
RFA: Genetics of Alcohol-Related Behavio
Novelty or 'sensation-seeking' can be measured by nose-poke or hole board behavior in which the animal places its nose or head ... Applicants are also encouraged to consider using existing batteries of recombinant inbred (RI) and recombinant congenic (RC) ... The funds set aside for this RFA are intended to support all aspects of projects funded, except for genotyping of the animals ... Such a test will help to establish which animal behavioral traits are most relevant to human alcoholism. Mapping of the QTL ...http://www.bio.net/bionet/mm/neur-sci/1994-December/015940.html
Frontiers | Enforced OX40 Stimulation Empowers Booster Vaccines to Induce Effective CD4+ and CD8+ T Cell Responses against...
Animals and Methods. Mice. Wild-type female C57BL/6 mice (8-10 weeks) were purchased from Charles River Laboratories ( ... Splenic memory (day 65) CD8+ T cells from SLP vaccinated CD45.1+ congenic mice were negatively enriched with magnetic sorting ... Mice were bred and housed under specific-pathogen-free conditions at the Central Animal Facility of Leiden University Medical ... were approved by the LUMC Animal Experiments Ethical Committee and conducted according to the Dutch Experiments on Animals Act ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00144/full
All animal experiments were performed in accordance with a protocol approved by the Institutional Animal Care and Use Committee ... Female and male C57BL/6 wild-type (expressing CD45.2) and congenic (expressing CD45.1) mice and GFP transgenic mice were ... Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): K.O. Asiedu, S. Koyasu, L.P. ... thus serving as a tool to measure new transplant strategies rather than relying on animal models. In humans, because of the ...http://clincancerres.aacrjournals.org/content/23/11/2759
Mast Cell-Dependent Down-Regulation of Antigen-Specific Immune Responses by Mosquito Bites | The Journal of Immunology
All animal care and experimentation were conducted in accord with the Pasteur Institute animal care and use committee ... Mast cell-sufficient (WBB6F1-+/+) (+/+) and control congenic mast cell-deficient (WBB6F1-W/Wv) (W/Wv) mice were purchased from ... To set-up animal models for Plasmodium infection, a majority of investigators use i.v. or intradermal routes for sporozoite ... Animals. Female C57BL/6, 6-8 wk old, were purchased from Roger Janvier. ...http://www.jimmunol.org/content/176/7/4141.full
MGI - Inbred Strains: C57BL
... towards the location of predisposing polygenes in quasi-congenic animal-models. Alcohol 13, 617-620. \par. Van Heyningen H. E ... in contrast with C57BL/10 and B10 congenic strains (Tam and Messner, 1996). C57BL/10. Congenic line B10.D2-n is highly ... Animal Sci. 45, 652-656. \par. Hoag W. G. (1963) Spontaneous cancer in mice. Ann. NY Acad. Sci. 108, 805-831. \par. Hoffman H. ... Animal Sci. 26, 781-787. \par. Curtis H. J. (1971) Genetic factors in aging. Adv. Genet. 16, 305-324. \par. Dagg C. P. (1966) ...http://www.informatics.jax.org/inbred_strains/mouse/docs/C57BL.shtml
Behavioural and neurobiological consequences of macrophage migration inhibitory factor gene deletion in mice | Journal of...
Animals. Adult congenic MIF−/− (KO) mice and MIF+/+ (WT) littermates on a C57BL/6 background were used for these experiments. ... We examined depressive-like behaviour in our animal model by means of the SPT, the FST and the TST. On day 1-8, the animals ... p , 0.05 compared to WT animals (ANOVA) (n = 10-16 animals per group) ... Behavioral animal models of depression. Neurosci Bull. 2010;26(4):327-37.View ArticlePubMedGoogle Scholar. ...https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-015-0387-4
The American Journal of Tropical Medicine and Hygiene | Volume 53, Issue 2
Challenge of the H-2 congenic mouse strains A.SW (H-2s) and A.CA (H-2f) with Trypanosoma cruzi yields different results. The A. ... At the coastal site on Assateague Island, B. burgdorferi was isolated from 4.2% of 475 animals sampled, including four species ... or LC-fed animals. We conclude that in rats, an FO-supplemented diet reduces the production capacity of IL-1 and IL-6 and ... CA animals die approximately 12 days postinfection, while A.SW mice survive indefinitely. A 45-kD protein (Tc45), an antigen ...http://www.ajtmh.org/content/journals/14761645/53/2
Bancroft GJ (1993). The role of natural killer cells in innate resistance to infection. Curr Opin Immunol 5: - PDF
Animals Form & Function Activity #4 page 1 AP BIOLOGY ANIMALS FORM & FUNCTION ACTIVITY #4 NAME DATE HOUR BODY DEFENSES ... Use of congenic and mutant mice show transfer to be H-2K restricted. Cell Immunol 78: Chomarat P, Rissoan MC, Banchereau J und ... Laboratory Animal Science 41:. 6 VII. Literatur 131 Gordon S, Lawson L, Rabinowitz S, Crocker PR, Morris L und Perry VH (1992 ... 1) Siderophores are bacterial proteins that compete with animal A) Antibodies. B) Red blood cells. C) Transferrin. D) White ...http://docplayer.net/1915664-Bancroft-gj-1993-the-role-of-natural-killer-cells-in-innate-resistance-to-infection-curr-opin-immunol-5-503-510.html
Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): Y.-J. Park, J.-M Lee, J.-O. ... B and C, NK1.1+ cells were isolated from CD45.1 congenic mice inoculated subcutaneously with EL4 tumor cells and were ... To explore the development of tumor-associated MDSCs, we used animal models of Her-2-expressing CT26 colon carcinoma and EL4 ... All experiments were approved by the Institutional Animal Care and Use Committee of Seoul National University (Seoul, Korea). ...http://cancerres.aacrjournals.org/content/73/18/5669
In Vivo Instruction of Suppressor Commitment in Naive T Cells | JEM
Animals and Peptide Delivery Pump Treatment.. BALB/c TCR-HA,RAG-2−/− mice, Thy1.1 BALB/c congenic TCR-HA,RAG-2−/− mice, TCR-HA, ... Animal care and all procedures were in accordance with the guidelines of the Animal Care and Use Committee of the Dana-Farber ... transgenic BALB/c nude mice were bred in our animal facility. Unmanipulated 7-8-wk-old BALB/c mice or 7-8-wk-old BALB/c TCR-HA, ... a protocol was designed to provide animals with a subimmunogenic regimen of peptide by implanting osmotic pumps that supplied ...http://jem.rupress.org/content/199/10/1401?ijkey=ebb9cd2764d456f1fd14c2c16e3f5173975bc85d&keytype2=tf_ipsecsha
Polysialic Acid, a Glycan with Highly Restricted Expression, Is Found on Human and Murine Leukocytes and Modulates Immune...
RMA tumors grow faster in ST8Sia IV−/− mice than in wild-type animals. Wild-type, ST8Sia IV−/−, and TCRβ−/− mice (negative ... For in vivo experiments, GFP+ congenic wild-type mice were used as donors so that engrafted lineages could be identified using ... Animals surviving more than 60 days were considered tumor-free; all deaths are plotted on the graph. There was a statistically ... Animals were sacrificed after 5 days and polySia/c-Kit-defined bone marrow subsets were monitored by flow cytometry. The change ...http://www.jimmunol.org/content/181/10/6850.long
Kv1.3 Deletion Biases T Cells toward an Immunoregulatory Phenotype and Renders Mice Resistant to Autoimmune Encephalomyelitis |...
In unimmunized animals, Kv1.3 KO and WT mice were found to have similar percentages of CD4+, CD8+, and CD4+CD8+ cells in the ... To this end, we isolated CD4+ T cells from WT and Kv1.3 KO CD45.2 mice and transferred these cells into CD45.1 congenic hosts ... 1A, 1B). Unimmunized animals were also found to have similar numbers of naive, TCM, and TEM cells in the spleen and lymph node ... Mean clinical scores of animals are depicted in the graph. Incidence of disease is shown in the upper left corner. (B) Kv1.3 KO ...http://www.jimmunol.org/content/188/12/5877
Cutting Edge: The Orphan Nuclear Receptor Nr4a1 Regulates CD8+ T Cell Expansion and Effector Function through Direct Repression...
Animals. C57BL/6J wild-type (WT) mice (000664), C57BL/6-Tg(TcraTcrb)1100Mjb/J (003831) OT1 mice, B6.SJL-Ptprca Pepcb/BoyJ ( ... 002014) CD45.1 mice, and Nr4a1−/− mice on a congenic C57BL/6J background (006187) were from The Jackson Laboratory. Mice were ...http://www.jimmunol.org/content/195/8/3515