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*  Osteopontin is a hematopoietic stem cell niche component that negatively regulates stem cell pool size | JEM
We observed a similar G0/G1 and S+G2/M percentage of Sca1+c-kit+lin− in the bone marrow of OPN+/+ and OPN−/− animals (S+G2/M ... Congenic competitive BM cells (Ly5.1) were prepared as single cell suspensions from male mice. A mixture of equal amounts of ... in primary animals and in transplanted animals, BM cells were stained with biotinylated lineage antibodies (CD3, Ter119 [BD ... seen in an OPN-deficient animal was not persistent when cells from that animal were transplanted into a wild-type background. ...
http://jem.rupress.org/content/201/11/1781
*  PLZF induces an intravascular surveillance program mediated by long-lived LFA-1-ICAM-1 interactions | JEM
To study the NKT cell recirculation pattern, we generated parabiotic mice with pairs of CD45.1 and CD45.2 congenic animals. ... Parabiotic mice do not exchange NKT cells. CD45.1 and CD45.2 congenic C57BL/6 mice joined in parabiotic pairs at 6 wk of age ... Thus, parabiotic mice harbored ,5-10% congenic NKT cells 2 mo after surgery. In contrast, the peculiar population of CD4− NKT ... we extracted them from the spleen or liver of Vα14-Jα18 transgenic mice and intravenously injected them into CD45 congenic RAG ...
http://jem.rupress.org/content/208/6/1179.full
*  A novel isoform of the Ly108 gene ameliorates murine lupus | JEM
More importantly, we identified a third protein isoform, Ly108-H1, which is absent in two lupus-prone congenic animals. ... The congenic Ly108−/− [129 × B6] mouse does not develop SLE, in contrast to the B6.129chr1b congenic mouse, which contains the ... Animal experiments were approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee. ... the disruption of the Ly108 gene in a congenic mouse ameliorates disease found in a very similar congenic mouse strain that ...
http://jem.rupress.org/content/208/4/811
*  Essential Roles of CD8+CD122+ Regulatory T Cells in the Maintenance of T Cell Homeostasis | JEM
12) and maintained in our animal facility. Breeding pairs of the C57BL/6CD45.1/CD45.1 congenic strain were obtained from ... Mice deficient in the interleukin (IL)-2/IL-15 receptor β chain, CD122, are model animals of such immune attacks and ... RAG-2-deficient mice with C57BL/6 genetic background were obtained from The Central Institute for Experimental Animals with ... The experimental protocol was approved by the Ethics Review Committee for Animal Experimentation of Nagoya University Graduate ...
http://jem.rupress.org/content/200/9/1123?ijkey=777c346b1f9cf373ed8762a73d4e0eb8a13c476d&keytype2=tf_ipsecsha
*  T follicular helper cells differentiate from Th2 cells in response to helminth antigens | JEM
FACS-purified cells (∼2 × 105) were injected i.v. into congenic animals, which were then immediately immunized with SEA into a ... Strikingly, we were unable to detect CXCR5+ PD-1+ IL-4/GFP+ CD4+ TFH cells in immunized B cell-deficient µMT/4get animals (Fig ... These animals were immunized with SEA, and 14 d later, draining LN cells were stained for PD-1 and the TFH markers CXCR5 and ... Animals, infections, and immunizations.. 4get (C.129-Il4tm1Lky/J) and 4get/KN2 mice were previously described (28). 4get/µMT ...
http://jem.rupress.org/content/206/5/991
*  Cbfβ2 deficiency preserves Langerhans cell precursors by lack of selective TGFβ receptor signaling | JEM
All animal experiments were performed in the animal facility at the RIKEN Center for Integrative Medical Sciences according to ... C57/BL6 mice congenic for the CD45 locus were purchased from Sankyo Labo Service Coro. CSFIR-iCre transgenic mice (JR019098) ... In addition, CD3+ DETCs, known as skin-specific γδT cells, were not present in the epidermis of Cbfb2m/2m animals (Fig. 1, A ... 4 A). In contrast, the majority of LCs were of host origin (CD45.2) in Cbfβ+/+ recipient animals (Fig. 4 A). However, in the ...
http://jem.rupress.org/content/early/2017/08/15/jem.20170729
*  Sirt1 ablation promotes stress-induced loss of epigenetic and genomic hematopoietic stem and progenitor cell maintenance | JEM
Animal work was approved by the NCI-Frederick Animal Care and Use Committee (ASP 09-066). ... For serial transplantation assays, 106 BM cells from pooled WT or SIRT1Δ/Δ donors were transferred into CD45.1+ congenic ... Recipient animal numbers three per group for primary transfer and four per group for secondary transfer. (B) Tertiary transfer ... Animals were monitored daily for signs of morbidity and euthanized when moribund. Kaplan-Meier survival curves were generated ...
http://jem.rupress.org/content/210/5/987
*  Protective influenza-specific CD8 T cell responses require interactions with dendritic cells in the lungs | JEM
Moreover, adoptive transfer of pDCs purified from the spleens of influenza virus-infected animals, unlike transfer of the ... BALB/c Thy1.1 congenic mice were a gift from R. Enelow (Dartmouth College, Hanover, NH) and J. Harty (University of Iowa, Iowa ... Experiments were conducted according to federal and institutional guidelines and were approved by the University of Iowa Animal ... into BALB/c Thy1.1 congenic mice. 24 h later, the host mice were infected with influenza virus. ...
http://jem.rupress.org/content/205/7/1635
*  Homozygous scid/scid;beige/beige mice have low levels of spontaneous or neonatal T cell-induced B cell generation. | JEM
The scid mutation occurred in the allotype congenic C.B-17 line, and up to 25% of C.B-17 scid mice spontaneously produce both T ... of animals. We have produced mice homozygous for both the scid and beige mutations. By contrast with C.B-17 scid mice, BALB/c ...
http://jem.rupress.org/content/177/1/191?ijkey=f9d9033f94a8e1b880907ad7d88d9cfc5935a538&keytype2=tf_ipsecsha
*  HIF-1α regulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment | JEM
Congenic CD45.1+ mice were injected i.p. with 1 ml thioglycollate. 3 d later, 5 × 106 MDSCs isolated from spleen of EL-4 tumor- ... All experiments with mice were approved by University of South Florida Animal Care and Use Committee. C57BL/6 and BALB/c female ... CD45.1+ congenic mice (B6.SJL-PtrcaPep3b/BoyJ), gp91phox−/− (B6.129S6-Cybbtm1Din), HIF-1αflox/flox (B6.129-Hif1atm3Rsjo/JE), ... H) Reconstitution of lethally irradiated CD45.1+ congenic mice with CD45.2+ BM from HIF-1α-deficient (HIF-1αflox/flox, Cre+/−) ...
http://jem.rupress.org/content/207/11/2439
*  In Vivo Instruction of Suppressor Commitment in Naive T Cells | JEM
Animals and Peptide Delivery Pump Treatment.. BALB/c TCR-HA,RAG-2−/− mice, Thy1.1 BALB/c congenic TCR-HA,RAG-2−/− mice, TCR-HA, ... Animal care and all procedures were in accordance with the guidelines of the Animal Care and Use Committee of the Dana-Farber ... transgenic BALB/c nude mice were bred in our animal facility. Unmanipulated 7-8-wk-old BALB/c mice or 7-8-wk-old BALB/c TCR-HA, ... a protocol was designed to provide animals with a subimmunogenic regimen of peptide by implanting osmotic pumps that supplied ...
http://jem.rupress.org/content/199/10/1401?ijkey=ebb9cd2764d456f1fd14c2c16e3f5173975bc85d&keytype2=tf_ipsecsha
*  Plasma glutamate-modulated interaction of A2AR and mGluR5 on BMDCs aggravates traumatic brain injury-induced acute lung injury ...
Animals.. Global A2AR homozygous KO mice and their WT littermates used in this study were developed in the laboratory of J.-F. ... congenic global A2AR KO mice on a C57BL/6 background were generated by backcrossing global A2AR KO on mixed (129-Steel×C57BL/6 ... Amino acid transporters: roles in amino acid sensing and signalling in animal cells. Biochem. J. 373:1-18. doi:10.1042/ ... 4 A). Consistent with these results in animal models, analysis by SDS-PAGE and immunoblotting with anti-mGluR5 antibodies of ...
http://jem.rupress.org/content/210/4/839
*  Type I interferons act directly on CD8 T cells to allow clonal expansion and memory formation in response to viral infection |...
P14 and OT-1 TCR transgenic mice that were on a B6 background were bred to B6 Ly5.1 mice to generate mice cells with a congenic ... All mice were maintained under specific pathogen-free conditions at the University of Washington animal care facility under the ... To assess these possibilities, we cotransferred large numbers of WT and IFN-IR0 P14 CD8 T cells into naive congenic mice and ... 60,000 each of WT (Thy1.2, Ly5.1) plus IFN-IR0 (Thy1.2, Ly5.2) P14 CD8 T cells were cotransferred into a congenic host (Thy1.1 ...
http://jem.rupress.org/content/202/5/637?ijkey=f2b051a5c0875f0f35199225236c92182cf94b79&keytype2=tf_ipsecsha
*  Control of Foxp3 stability through modulation of TET activity | JEM
Congenic mice (strain B6.SJL-PtprcaPepcb/BoyJ) were obtained from The Jackson Laboratory. Tet2−/−Tet3fl/flCD4-Cre, Tet2fl/fl ... Similar to T reg cells from CNS2-deficient animals, T reg cells from Tet2/Tet3 double-deficient mice show a marked impairment ... 105 CD45.2+ T reg cells mixed with 106 CD4+ naive T cells FACS sorted from CD45.1+ congenic mice were injected intravenously ... All breeding and experiments were reviewed and approved by the Institutional Animal Care and Use Committee of the La Jolla ...
http://jem.rupress.org/content/213/3/377
*  In Vivo Survival and Homeostatic Proliferation of Natural Killer Cells | JEM
d) Splenocytes were harvested from Rag−/− animals (open symbols) and from Rag−/−γC−/− animals which were transferred with Rag ... Rag-2−/−/γC−/− double deficient (Rag−/−γC−/−), CD45.1+ Rag-2−/− mice (CD45 congenic Rag−/−), and IL-15−/− mice were obtained ... 7 d after transfer, these animals and control Rag−/− animals were challenged with poly (I:C) or left as controls. Spleens of ... Splenocytes from Rag−/− animals were harvested, CFSE labeled, and adoptively transferred into Rag−/−γC−/− hosts. Host animals ...
http://jem.rupress.org/content/197/8/967
*  OX40 is required for regulatory T cell-mediated control of colitis | JEM
Naive CD4+CD25−CD45RBhi T cells were isolated from congenic B6.SJL-Cd45 (CD45.1+) mice, WT or OX40−/− C57BL/6 mice and injected ... We thank N.Rust for assistance with cell sorting, R.Stillion for histology, and the staff of our animal facility. We thank our ... C) BM cells from WT congenic mice (CD45.1+) mixed in equal proportion with BM cells from OX40−/− mice (CD45.2+) were injected i ... Wild-type C57BL/6, congenic B6.SJL-Cd45, C57BL/6 RAG−/−, C57BL/6 OX40−/− mice, and Foxp3-GFP knock-in mice were bred and ...
http://jem.rupress.org/content/207/4/699
*  Cryptococcus neoformans STE12α Regulates Virulence but Is Not Essential for Mating | JEM
Groups of mice were infected with an F1 prototroph of the ste12α disruptant (TYCC245F1) and the wild-type congenic strain (B- ... it was of interest to test the role of STE12α in virulence using an animal model. ... The virulence of the STE12α-reconstituted strain was compared with that of the wild-type congenic strain, B-4500. Fig. 6 B ... B-4500 (MATα) is a wild-type congenic strain of B-4476 (MATa) previously used to investigate genetic association of mating type ...
http://jem.rupress.org/content/191/5/871
*  Asymmetry in skeletal distribution of mouse hematopoietic stem cell clones and their equilibration by mobilizing cytokines | JEM
1 A) were used to study the response within the same animal. The animals were mobilized 8.5 or 11 mo after transplantation, and ... CD45 congenic B6 and W41 strains were used to discriminate between donor and recipient cells and to distinguish young and aged ... Experiments with parabiotic animals demonstrated quite a slow exchange of HSC pools during an observation time up to 22 wk ( ... Recipients of young cells belong to one cohort of seven animals. Recipients of old LSK48−150+ cells relate to two cohorts (n = ...
http://jem.rupress.org/content/211/3/487
*  A Critical Role for Induced IgM in the Protection against West Nile Virus Infection | JEM
20 animals were included in the study. 8 survived and 12 died after infection. Five of the animals that died had identical ... Compared with congenic wild-type mice, no significant difference in WNV infection was observed in sIgM−/− mice during the first ... The number of animals were n = 28 for wild-type mice and n = 24 for sIgM−/− mice. The mean survival times (in days) were 21.8 ... To address this directly, we compared WNV infection in wild-type and congenic sIgM−/− C57BL/6 mice that lack the ability to ...
http://jem.rupress.org/content/198/12/1853
*  The aryl hydrocarbon receptor controls cell-fate decisions in B cells | JEM
All animals were maintained and euthanized as per the Memorial Sloan Kettering Cancer Center Research and animal resource ... For BM chimeras, WT CD45.1 congenic and AhR−/− mice (or WT CD45.2 C57BL6/J; 6-8-wk-old females) were used as a source of BM ... Serum from PBS/BSA-infected and PR8-infected mixed chimeras was taken by cheek bleeding mice (Goldenrod animal lancet 5 mm; ... S4 B), demonstrating that congenic differences do not per se skew B cell development. Splenic B cell chimerism was also ...
http://jem.rupress.org/content/214/1/197
*  Annular PIP3 accumulation controls actin architecture and modulates cytotoxicity at the immunological synapse | JEM
The animal protocols used for this study were approved by the Institutional Animal Care and Use Committee of Memorial Sloan- ... Lymphocytes from these mice were mixed with irradiated congenic splenocytes pulsed with 5 µM moth cytochrome c88-103 peptide ( ...
http://jem.rupress.org/content/210/12/2721
*  A Coordinated Change in Chemokine Responsiveness Guides Plasma Cell Movements | JEM
Data from animals reconstituted with wild-type IgHa bone marrow and CXCR4+/+ IgHb fetal liver are shown in white (IgHa) and ... Staining for a congenic marker, CD45.1, to distinguish donor and recipient cells established that ,90% of B220+ B cells (97 ± 2 ... 6 C). By day 7, the number of cells in blood had decreased in both wild-type and mutant animals, but numbers in the blood of ... Lethally irradiated 129 (IgHa) strain mice were reconstituted with a mixture of IgHa congenic C57BL/6 bone marrow and CXCR4+/ ...
http://jem.rupress.org/content/194/1/45?ijkey=8f7358e7b072b59dd1c737988b55bcccffdaa421&keytype2=tf_ipsecsha
*  Splenic T Zone Development Is B Cell Dependent | JEM
7). Transgenic animals were made that carry the coding region of the LTα gene fused to the segment of the LTβ gene encoding the ... 6 A), a phenotype similar to congenic LTα-deficiency (see Figs. 2 and 6 B). When the antagonist treatment was delayed until day ... In wild-type animals, we have provided evidence that LTα1β2 expression on B cells is cyclical, being induced by CXCL13 to ... 5 A). Although the LTβR-Ig is likely to have persisted in the serum of these animals for several weeks (40), the importance of ...
http://jem.rupress.org/content/194/11/1649
*  CCR7 Signals Are Essential for Cortex-Medulla Migration of Developing Thymocytes | JEM
AND-TCR transgenic mice (10), HY-TCR transgenic mice (11), 2C-TCR transgenic mice (12), and B6.SJL-Ptprca congenic mice (CD45.1 ... MHC class I/class II double-deficient mice were bred in our animal facility by crossing MHC class I-deficient B6 background ... Mice were bred and maintained under specific pathogen-free conditions in our animal facility, and experiments were performed ... with consent from the Animal Experimentation Committee of the University of Tokushima. ...
http://jem.rupress.org/content/200/4/493
*  T Helper Subset Differentiation in the Absence of Invariant Chain | JEM
All incubations in the absence of antigen resulted in cytokine recoveries ,7 U/ml, except for one β2m −/− animal with an IFN-γ ... but congenic for H-2d and H-2k, were used where designated. ... Cohorts of β2m −/− and Ii +/− animals, together with ... We also thank R. Jaenisch for use of β2m −/− mice, N. Killeen for help with construction of TCR transgenic animals, N. ... B) Ii −/− mice on a resistant background were crossed to βm −/− to create double mutant animals and infected with promastigotes ...
http://jem.rupress.org/content/185/1/31