Ultimately, the effect of emerging (non-thermal) technologies on different food components (proteins, carbohydrates, lipids, ... Tahergorabi, R.; Hosseini, S.V. Proteins, peptides, and amino acids. In Nutraceutical and Functional Food Components: Effects ... which breaks down protein into smaller molecules that, upon digestion, yield the same amino acids as the original proteins [117 ... Food proteins are highly polymeric and complex molecules that are comprised of 20 different building units (the amino acids) ...

The fungus-originating CaM of the FRCaMP indicator version with a deleted M13-like peptide did not interact with the cytosolic ... which allowed the simultaneous detection of calcium transients and the heterodimerization of bJun/bFos interacting proteins in ... to the metazoa-originating CaM of the similarly truncated version of the GCaMP6s indicator with a deleted M13-like peptide. ... Compared to the R-GECO1 indicator in vitro, the purified protein FRCaMP had similar spectral characteristics, brightness, and ...

Protein-, (Poly)peptide-, and Amino Acid-Based Nanostructures Prepared via Polymerization-Induced Self-Assembly by Spyridon ... Proteins and peptides, built from precisely defined amino acid sequences, are an important class of biomolecules that play a ... Proteins and peptides, built from precisely defined amino acid sequences, are an important class of biomolecules that play a ... In this review article, the different strategies employed for direct construction of protein-, (poly)peptide-, and amino acid- ...

This review aims to analyze various peptide-copper complexes and the mechanism of their action. ... peptides and proteins) playing an essential role in many biological and physiological pathways in the human body. The resulting ... The β-amyloid peptide is an amyloid precursor protein (APP protein) fragment that consists of 40-43 amino acid residues. Aβ40 ... Spike (S) Protein. The spike protein, which consists of 1273 amino acid residues, is present on the protein-lipid envelope of ...

... nucleic acids, lipids, and meteorite components that induced symmetry imbalance. ... probably selected for their efficient catalytic activities during evolution from large libraries of vesicles containing amino ... A Few Experimental Suggestions Using Minerals to Obtain Peptides with a High Concentration of L-Amino Acids and Protein Amino ... amino acid was plausibly improved by CPL [39] or from the presence of enantiomorphic crystals. Thus, amino acid sequences ...

... we mapped a total of 1204 acetylated peptides corresponding to 603 proteins, including chromatin modifying proteins and ... Changes in overall protein expression as well as histone and protein acetylation were measured following Hdac1 deletion or ... We found changes in overall protein abundance and increased acetylation of histones and non-histone proteins, many of which ... Hyperacetylated proteins were involved in processes such as transcription, RNA metabolism and DNA damage repair (DDR). The DDR ...

C37 is a 37-amino acid peptide containing sequences from the C-terminal region of the HIV transmembrane protein gp41. C37 ... The wild-type protein from the alga contained an uncommon amino acid of 151.05 Da at position 31 that was replaced by alanine ( ... Two out of 12 β-strands (16 amino acids) swap from one monomer to the other to form a β-prism of three four-stranded sheets. ... Amino acids located within beta strands are colored red and the black arrows correspond to secondary structure. X represents an ...

In the present study, the thermal stability of α-glucosidase was significantly improved by constructing cyclized proteins. We ... indicating that the rigidity of the cyclized protein increased. This study provides an efficient method for improving the ... Catcher is able to specifically bind the Tag peptide and catalyze the formation of an isopeptide bond between two amino acid ... Primers used to construct the cyclized proteins; Table S3: Amino acid sequence of cyclized protein. ...

The entire gene codes for a protein consisting of 661 amino acids, comprising a putative signal peptide and an active domain. ... Antimicrobial peptides (AMPs) are naturally occurring innate immune response peptides with therapeutic [...] Read more. ... Antimicrobial peptides (AMPs) are naturally occurring innate immune response peptides with therapeutic potential. However, ... Proteins are shown using a "sausage" cartoon representation where the thickness of the tube indicates the B-factor. The color ...

... we find that current methods are not well suited to distinguish lncRNAs or parts thereof from other non-protein-coding input ... Eukaryotes (both protein-coding and non-coding genes). peptide length, amino acid composition, predicted secondary structure ... The selected features consist of peptide length (four features), amino acid composition (20 features), predicted protein ... Peptide length. The hypothetically translated amino acid (AA) sequence of a given ORF can be analyzed for physico-chemical and ...

Proteases (EC 3.4.21.62) are enzymes that perform proteolysis by hydrolysing the peptide bonds that link amino acids together ... Transglutaminases (E.C. 2.3.2.13, protein-glutamine-γ-glutamyl transferase) are a class of transferase enzymes which catalyse ... Acid hydrolysis of sucrose results in the 50% conversion of sucrose to invert sugar. Moreover, the acid hydrolysis product also ... This enzyme system consists of endoxylanases, β-xylosidases, ferulic acid esterase, p-coumaric acid esterase, acetylxylan ...

G protein-coupled receptors (GPCRs) belong to the largest class of drug targets. Approximately half of the members of the human ... Umami molecules are proteogenic amino acids or peptides, but also small molecules [129,130,131]. Bitter molecules include ions ... D-amino acids (e.g., D-Tryptophane), proteins (e.g., thaumatin), plant-derived sweeteners (e.g., neohesperidin dihydrochalcone ... Cho, M.J.; Unklesbay, N.; Hsieh, F.H.; Clarke, A.D. Hydrophobicity of bitter peptides from soy protein hydrolysates. J. Agric. ...

This review will critically discuss both the experimental approaches and prominent protein candidates identified. Many proteins ... including synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in ... Numerous proteins regulating the rearrangement of the cytoskeleton are indicative of the functional and structural dynamics of ... the interaction of presynaptic proteins with their targets and opens novel insights into the functional role of these proteins ...

... amino acid identity), with the exception of the polymerase basic 1 (PB1) protein (58% amino acid identity) [31]. Despite this, ... T cells express a clonal T cell receptor (TCR), which recognizes linear fragments of viral peptides that are 9-15 amino acids ... T cell epitopes in the PB1 protein show complete amino acid conservation or only slight variation amongst influenza A and B ... and recombinant peptide approaches for mosaic of conserved peptides or NP with M2e proteins. Viral vectors have different ...

... of 15-mer peptides that cover the entire amino acid sequence of the respective human protein in steps of 11 amino acids. All ... Although such Level 3-reactive T cells recognize autoantigens that in terms of amino acid sequence are shared between tumor- ... Melanocyte protein Pmel 17gp100 (PM-GP100), and NY-ESO-1 (PM-NYE). All five MA peptide pools were tested at a final ... CEF peptides, was not impaired compared to the HC cohort (Figure S1F). The CEF peptide pool consists of immune dominant ...

... study is described to identify novel potential peptides designed by employing a database of non-natural amino acids. For this ... Finally, the latter peptides in complex with PBX1 protein and DNA were exploited to run MD simulations and the ΔGbinding ... HOX proteins execute their activity by exploiting a cooperative binding to PBX proteins and DNA. Therefore, an increase or ... According to these results, the peptides were point-mutated and used for Molecular Dynamics (MD) simulations in complex with ...

A signature peptide for each specific splice variant of Lamin A/C was selected. A LC-MS/MS assay based on the selected ... Lamin A/C cumulative protein expression of all variants is reported by semi-quantitative Western blotting. To date, there have ... signature peptides and their labeled internal standards was established to measure the expression of Lamin A/C transcript ... Lamin A/C proteins have key roles in nuclear structural integrity and chromosomal stability. ...

51] developed an 11-amino acid peptide, based upon the apoB protein that successfully facilitated the transport of siRNA ... an amino-bridged nucleic acid (AmNA)-modified ASO showed greater intercellular stability, while retaining the ability to lower ... These are small peptides that induce B-cell activation while being too small to elicit the potentially deleterious T-cell ... Specific peptides can also be used to improve siRNA delivery to the nervous system. Spencer et al. [ ...

... as they lie within CD8+ T cell epitopes that are commonly targeted in individuals and are predicted to abrogate HLA-peptide ... Epitope mutants comprising any ambiguous amino acid were discarded. The count of each identified epitope mutant, i.e., the ... This is because a loss of peptide-HLA binding is irreparable since such a peptide would not be expressed on the cell surface ... Of the 83 epitope mutants, 16 are derived from the spike (S) protein, which could be particularly important given that most ...

Gluten peptides enter the pancreas where they affect the morphology and might induce beta-cell stress by enhancing glucose- and ... Moreover, studies have demonstrated that gluten peptides, after crossing the intestinal barrier, lead to a more inflammatory ... The major amino acid constituents of gluten are proline, glutamine, and hydrophobic amino acids [9], which make gluten ... Short Chain Fatty Acids Prevent High-fat-diet-induced Obesity in Mice by Regulating G Protein-coupled Receptors and Gut ...

In 31 epitope candidates (78%) amino acid differences were detected, and in 17 (43%) of the epitope candidates the ... However, limited data are available about sequence variability in CD8+ T cell epitope regions of mumps virus (MuV) proteins. ... The eluted peptides that could be assigned to MuV proteins were selected for final confirmation by MS analysis using synthetic ... MuV Protein. Amino Acid Sequence. X-Mer. Protein Location. Described [Ref]/Novel. JL Eluted. Seq Variation. Reduced Binding. ...

... and results in several amino acids-tyrosine, alanine, and lysine [28]-from the digestion of proteins. Bromelain has also been ... when it comes to stimulating collagenase and breaking down collagen by dissolving the peptide bonds that hold their proteins ... which breaks other proteins apart by cutting the chains of amino acids. Even more specifically, bromelain is a cysteine ... meaning that it breaks apart proteins wherever they have a cysteine amino acid [21]. Bromelain, the pineapple protease, ...

Amino acid sequences of viral proteins were modified to increase their expression level and optimize their immunological ... peptide, or protein, Lentiviral vector and dendritic cell have been used in numerous clinical trials. Despite the major ... amino acids 676-860) were removed to obtain a soluble form of the HIV-1 envelope glycoprotein, region 500-534 containing the ... and Nef proteins was used. Peptides were synthesized at the Research Institute of Ultra Pure Biologicals (St. Petersburg, ...

The contributions of individual amino acid residues or groups of amino acids to antioxidant activities of some food protein ... that will efficiently generate potent antioxidant peptide mixtures from food proteins without the need for costly peptide ... The contributions of individual amino acid residues or groups of amino acids to antioxidant activities of some food protein ... Chemometric Analysis of the Amino Acid Requirements of Antioxidant Food Protein Hydrolysates by Chibuike C. Udenigwe ...

Given the role of amino-acid vibrations and reorientation in the dehydration process at the protein-water-substrate interface, ... As might be expected, the 40 proteins that gave rise to the 50 affected phospho-peptides mostly function in processes that have ... Surprisingly, our simulations revealed that single amino-acid mutations gave rise to a quenching of protein vibrations ... While a number of the more-affected sites resemble the CTD in amino acid sequence and are likely direct CDK12 substrates, other ...

Static electric field strengths of 0.001 V/nm and 0.002 V/nm induced conformational changes in the protein but had no ... This study demonstrated that it is necessary to gain insight into protein dynamics under external electric field stress, in ... modeling approach was applied to evaluate the effect of external electric field on gliadin protein structure and surface ... They both are characterized by their high content of glutamine and proline amino acids [1]. The presence of these amino acids ...

... of all possible amino acid pairs in a protein, only about 2% are in contact [140]. Prediction of post-translation modifications ... low sensitivity in protein and peptide detection, and faltered metabolite measurement by tandem mass spectrometry, etc. [106, ... Wei, Z.-S.; Han, K.; Yang, J.-Y.; Shen, H.-B.; Yu, D.-J. Protein-protein interaction sites prediction by ensembling SVM and ... Wei, Z.-S.; Yang, J.-Y.; Shen, H.-B.; Yu, D.-J. A cascade random forests algorithm for predicting protein-protein interaction ...

While computational protein design has enabled the development of symmetric protein complexes with spherical shapes and hollow ... Peptides have also been applied to self-assembly, and it is of interest to explore such short sequences as building blocks of ... Coiled-coil peptides are promising subunits as they have a symmetric structure that can undergo further assembly. Here, an α- ... The design strategy allowed breaking the side chain conformational symmetry within the peptide dimer that formed the building ...

This protein migrates in several bands by Western blot analysis (WB). Interestingly, PNGase F treatment of human brain ... This resembles the β-amyloid precursor protein (APP) in Alzheimer disease (AD), which can be physiologically processed by α-, β ... Nevertheless, there is a strong correlation between the neurotoxicity caused by prion proteins and the blockade of their normal ... The human cellular prion protein (PrPC) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N- ...

... ferulic acid, ergothioneine, pyroglutamyl leucine, pyranonigrin A, resistant proteins, deferriferrichrysin, polyamines, ... angiotensin I-converting enzyme inhibitor peptides, 14-dehydroergosterol, beta-glucan, biotin, and citric acid. This review ... Recent research has elucidated that koji contains medicinal substances such as Taka-diastase, acid protease, koji ... glycosylceramide, kojic acid, oligosaccharides, ethyl-α-d-glucoside, ...