The facultative anaerobic bacterium Escherichia coli is frequently forced to adapt to changing environmental conditions. One important determinant for metabolism is the availability of oxygen allowing a more efficient metabolism. Especially in large scale bioreactors, the distribution of oxygen is inhomogeneous and individual cells encounter frequent changes. This might contribute to observed yield losses during process upscaling. Short-term gene expression data exist of an anaerobic E. coli batch culture shifting to aerobic conditions. The data reveal temporary upregulation of genes that are less efficient in terms of energy conservation than the genes predicted by conventional flux balance analyses. In this study, we provide evidence for a positive correlation between metabolic fluxes and gene expression. We then hypothesize that the more efficient enzymes are limited by their low expression, restricting flux through their reactions. We define a demand that triggers expression of the demanded enzymes
d,e) Aerobiosis analysis. The cladograms report the taxa (highlighted by small circles and by shading) showing different ...
by-product formation rates and glucose uptake rates of mutants with linear electron transport chain at different aerobiosis ... Dephosphorylation and therefore inactivation of ArcA started at lower aerobiosis levels than in the wild-type strain. Notably, ... Determination of by-product formation and glucose uptake of mutants with linear respiratory chain at different aerobiosis ... Determination of by-product formation and glucose uptake of mutants with linear respiratory chain at different aerobiosis ...
Aerobiosis * Anaerobiosis * Carbon Dioxide / metabolism * Cyclohexanols / metabolism* * Kinetics * Pseudomonas / growth & ...
Aerobiosis. Altitude*. Animals. Body Weight. Demography. Oxygen Consumption. Peromyscus / physiology*. Temperature*. From ...
Aerobiosis. Anaerobic Threshold* / physiology. Energy Metabolism. Glycogen / metabolism. Humans. Lactates / blood*, metabolism ...
Aerobiosis * Anaerobiosis * Cystic Fibrosis / complications* * Cystic Fibrosis / metabolism* * Cystic Fibrosis / microbiology * ...
Anaerobic growth corresponds to an aerobiosis value of 0%. An aerobiosis value of 100% is defined as the steady state with the ... Using the aerobiosis scale, results of different laboratories using different reactors can be compared. The aerobiosis scale ... the citric acid cycle shows the switch from its branched form at low aerobiosis values to the cyclic form at high aerobiosis ... aerobiosis units. The aerobiosis scale allows the reproducible analysis of microaerobic states on the physiological (Alexeeva ...
2002) Aerobiosis increases the genomic guanine plus cytosine content (GC%) in prokaryotes. J Mol Evol 55:260-264. ...
Cultivo y aislamiento en agar sangre a 37º en aerobiosis. Colonias blancas cremosas, lisas brillantes (24hs.). Colonias rojo ...
The growth of these mutants was then tested under different conditions (aerobiosis and aerobiosis enriched with CO2) and on ... or of arginine/ml in aerobiosis or CO2-enriched air. Aerobiosis was obtained by incubation in ordinary air. To calibrate the ... All these mutants were isolated under CO2-enriched growth conditions (aerobiosis enriched with 4% CO2), since CPS-A activity ... These mutants displayed the characteristic growth dependency on the CO2 supply; on DLA medium, no growth in aerobiosis was ...
2009) Late Archean molecular fossils from the Transvaal Supergroup record the antiquity of microbial diversity and aerobiosis. ... 2008) Methylhopane biomarker hydrocarbons in Hamersley Province sediments provide evidence for Neoarchean aerobiosis. Earth ...
aerobiosis - life sustained in the presence of air or oxygen. 12.. life - an account of the series of events making up a ...
2). In wild-type cells, ethanol (EtOH) production ceased at 70% aerobiosis (qEtOH , 0.05 mmol · g−1 · h−1); in contrast, the ... This high ratio was observed at up to 90% aerobiosis. Above this range, the NADH/NAD ratio in the ΔarcA cultures, much as in ... 6). Moreover, the change in the NADH/NAD ratio in response to a very low oxygen supply (5 to 10% aerobiosis) in the ΔarcA ... Much as in the wild-type strain, an increase in the extent of aerobiosis led also to a gradual decrease of the rate of ...
Species Differences in Edema Formation and Energy Metabolism of the Heart Muscle During Aerobiosis and Anaerobiosis +). Methods ...
Relationships between mean SR and possible driving factors among sites.SR represented soil respiration; The driving factors were soil temperature (ST) at 5 cm d
Annual SR for the six sites.SR means soil respiration. Different letters denote significant differences among means (á = 0.05) as determined by Turkeys
Aerobiosis is characteristic of the overwhelming majority of animals, plants, and... Explanation of aerobiotic ... Aerobiosis. (redirected from aerobiotic). Also found in: Dictionary, Thesaurus, Medical. aerobiosis. [‚e·rō‚bi′ō·səs] (biology ... Aerobiosis. life in the presence of free oxygen. Aerobiosis is characteristic of the overwhelming majority of animals, plants, ...
Ydenberg, R. C. and Clark, C. W. (1989). Aerobiosis and anaerobiosis during diving by Western Grebes: an optimal foraging ...
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... but it is required for normal growth in aerobiosis. As expected, the inactivation of ASC1 in the hap1−hem1− strain W3/H/C ... These results clearly show that only the ORF is involved in normal growth in aerobiosis and in the inability to grow on TE in ... because cells in which HAP1 is inactivated do not present an obvious phenotype in aerobiosis and heme-sufficient conditions. In ...
Aerobiosis analysis under aerobic, anaerobic, and microaerobic conditions. The cladogram shows the aerobiosis analysis of the ... aerobiosis directory. The two files represent the taxonomic tree of the HMP project and the results of LEfSe executed on the ...
All plates were incubated at 35 °C under aerobiosis for 24 h. ... C under aerobiosis for 24 h and isolated colonies sub-cultured ...
The samples were incubated under aerobiosis, at 37 °C, for 24 h. All assays were performed in duplicate. Chloroform was used in ... The samples were incubated under aerobiosis, at 37 °C, for 24 h. The evaluation of antibacterial activity of the substances 4, ...
Effect of aerobiosis and temperature onsod::lacZ expression in CAL2. Mid-log-phase CAL2 cells were grown for 2.5 h under the ... In the present study, a loss of viability of more than 4 orders of magnitude within 1 h of aerobiosis was noted. Apart from ... Furthermore, while CAL1 is highly sensitive to aerobiosis, it demonstrates a modest tolerance to exogenous ROS, equivalent to ...