Recombinant Human Leptin Treatment Does Not Improve Insulin Action in Obese Subjects With Type 2 Diabetes | Diabetes
Leptin impairs metabolic actions of insulin in isolated rat adipocytes. J Biol Chem 1997;272:10585-10593pmid:9099705. ... Brown MS, *Goldstein JL. . Leptin reverses insulin resistance and diabetes mellitus in mice with congenital lipodystrophy. ... Adipocyte JAK2 Regulates Hepatic Insulin Sensitivity Independently of Body Composition, Liver Lipid Content, and Hepatic ... Leptin impairs insulin signaling in rat adipocytes. Diabetes 2004;53:347-353pmid:14747284. ...http://diabetes.diabetesjournals.org/content/60/5/1474.full
Interactions Between Leptin and the Human Sympathetic Nervous System | Hypertension
1 Although extra-adipocyte sites of leptin production are well documented,2-5 it is the leptin released by adipocytes and ... 19 Brown adipose tissue, certainly, is spared from arterial baroreflex-mediated sympathetic activation,35 such as is operative ... Hardie LJ, Guilhot N, Trayhurn P. Regulation of leptin production in cultured mature white adipocytes. Horm Metab Res. 1996; 28 ... On the first point, direct sympathetic innervation of adipocytes does seem to exist,34 although there has previously been ...http://hyper.ahajournals.org/content/41/5/1072
The secretome of brown adipose tissue
... and primary brown adipocytes compared to primary white adipocytes. In vivo studies were performed to evaluate physiological ... To identify brown adipocyte secreted proteins a signal-sequence trap method was used. All genes identified were cloned and ... Open this publication in new window or tab ,,Effects of differentiation on gene expression of certain brown adipocyte-secreted ... in vivo that could be expected to show similar expression patterns as norepinephrine-treated brown adipocytes did not do so. ...http://su.diva-portal.org/smash/record.jsf?pid=diva2:714076
Serotonin prevents differentiation into brown adipocytes and induces transdifferentiation into white adipocytes
... ... Differentiated HIB1B brown adipocytes treated with serotonin had reduced levels of the thermogenic markers uncoupling protein 1 ... In parallel, serotonin led to 3-6-fold reduction in the gene expression of brown adipocyte differentiation markers, that is, ... Non-differentiated HIB1B cells and differentiated HIB1B brown adipocytes were treated with serotonin and their metabolism and ...https://insights.ovid.com/pubmed?PMID=29081505
Synergism between cAMP and PPAR Signalling in the Initiation of UCP1 Gene Expression in HIB1B Brown Adipocytes
... adipocyte differentiation, inflammation, cancer, lung diseases, neurodegenerative disorders, and obesity. ... Signalling in the Initiation of UCP1 Gene Expression in HIB1B Brown Adipocytes. H. Y. Chen, Q. Liu, A. M. Salter, and M. A. ... Signalling in the Initiation of UCP1 Gene Expression in HIB1B Brown Adipocytes," PPAR Research, vol. 2013, Article ID 476049, 8 ...https://www.hindawi.com/journals/ppar/2013/476049/cta/
Frontiers | TGF-β/Smad3 Signaling Regulates Brown Adipocyte Induction in White Adipose Tissue | Endocrinology
Here we review the data supporting this phenomenon and put into perspective the promise of conversion of white fat to a brown ... Here we review the data supporting this phenomenon and put into perspective the promise of conversion of white fat to a brown ... described an important role played by the TGF-beta/Smad3 signaling pathway in modulating the appearance of brown adipocytes in ... described an important role played by the TGF-beta/Smad3 signaling pathway in modulating the appearance of brown adipocytes in ...https://www.frontiersin.org/articles/10.3389/fendo.2012.00035/full
A high-throughput, image-based screen to identify kinases involved in brown adipocyte development | Science Signaling
A high-throughput, image-based screen to identify kinases involved in brown adipocyte development ... A high-throughput, image-based screen to identify kinases involved in brown adipocyte development ... A high-throughput, image-based screen to identify kinases involved in brown adipocyte development ... A high-throughput, image-based screen to identify kinases involved in brown adipocyte development ...http://stke.sciencemag.org/content/10/466/eaaf5357.full
Directed differentiation of human pluripotent stem cells into brown adipocytes - Differentiation protocol - LifeMap Discovery
This protocol describes an effieicent method to generate brown adipocytes using hematopoietic cytokine cocktails. ...https://discovery.lifemapsc.com/stem-cell-differentiation/protocols/adipose-differentiation-directed-differentiation-of-human-embryonic-stem-cells-into-brown-adipocytes?TCCellID=597c5bf5-3ce8-4efb-8271-3fdc658b22c5
Phospholipase C-related Catalytically Inactive Protein Is a New Modulator of Thermogenesis Promoted by β-Adrenergic Receptors...
6E), suggesting high lipolytic activity in Prip-KO brown adipocytes. In β-AR-stimulated cultured brown adipocytes from Prip-KO ... Primary Culture of Mouse Brown Adipocytes and Oil Red O Staining Brown adipocyte precursors were isolated using a previously ... lipolytic products can activate Ucp1 gene expression in brown adipocytes (43). In Prip-KO cultured primary brown adipocytes, ... The culture medium was changed every 2 days until the differentiated cells resembled brown adipocytes. The primary brown ...http://pubmedcentralcanada.ca/pmcc/articles/PMC4759193/
Inhibitory Effects of Toll-Like Receptor 4, NLRP3 Inflammasome, and Interleukin-1β on White Adipocyte Browning | Springer for...
... on white adipocyte browning. We report that TLR4 activation by lipopolysaccharide repressed white adipocyte browning in ... TLR4 NLRP3 inflammasome IL-1β uncoupling protein 1 beige adipocytes brown adipocytes β3-adrenergic receptor ... Mitochondria in white, brown, and beige adipocytes. Stem cells International 2016: 6067349.CrossRefPubMedCentralPubMedGoogle ... Intrinsic properties of brown and white adipocytes have differential effects on macrophage inflammatory responses. Mediators of ...https://rd.springer.com/article/10.1007/s10753-017-0718-y
Hibernoma formation in transgenic mice and isolation of a brown adipocyte cell line expressing the uncoupling protein gene. -...
Most of the transgenic mice developed brown fat tumors (hibernomas) in their interscapular brown adipose tissue. Hibernoma ... All of the tumor tissue expressed the brown fat-specific uncoupling protein (UCP) gene as well as the aP2 gene. Several of the ... used to establish cultured cell lines and at least one of these lines can be induced to differentiate into brown adipocytes. ... The cultured adipocytes express mRNA for UCP upon stimulation with N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate, ...https://www.semanticscholar.org/paper/Hibernoma-formation-in-transgenic-mice-and-of-a-the-Ross-Choy/c390ded822b4761e6477c7741e0e5ab13c1dcbe7
Na+-dependent, alpha-adrenergic mobilization of intracellular (mitochondrial) Ca2+ in brown adipocytes.
... Connolly, E ... The existence and significance of a hormone-sensitive, rapidly mobilizable intracellular pool of Ca2+ in hamster brown-fat ...http://kau.diva-portal.org/smash/record.jsf?pid=diva2:657586
Alpha 1-adrenergic inositol trisphosphate production in brown adipocytes is Na+ dependent.
... Nånberg, Eewa Uppsala universitet, ... In order to investigate the ionic requirements for inositol trisphosphate production, brown adipocytes were prelabelled with ...http://kau.diva-portal.org/smash/record.jsf?pid=diva2:657597
De Novo Reconstruction of Adipose Tissue Transcriptomes Reveals Long Non-coding RNA Regulators of Brown Adipocyte Development
... ... Brown adipose tissue (BAT) protects against obesity by promoting energy expenditure via uncoupled respiration. To uncover BAT- ... lnc-BATE1 inhibition impairs concurrent activation of brown fat and repression of white fat genes and is partially rescued by ... We show that lnc-BATE1 binds heterogeneous nuclear ribonucleoprotein U and that both are required for brown adipogenesis. Our ...https://dr.ntu.edu.sg/handle/10220/39722
Assessment of Brown Adipocyte Thermogenic Function by ...
Here we provide protocols for measuring respiration in adherent intact and plasma membrane permeabilized brown adipocytes using ... A key parameter in assessing brown adipocyte thermogenic capacity is mitochondrial uncoupling as determined by respiration. ... Brown adipose tissue (BAT) has the unique ability to dramatically increase mitochondrial uncoupled fuel oxidation for ... Figure 2. Representative images of pre-adipocytes 24 h after isolation and mature brown-adipocytes after 7 days of ...https://en.bio-protocol.org/e1641
"Brown and Beige Adipocytes: Effects of Inflammation and Nutritional In" by Jiyoung Bae
... the roles of PRR activation in brown adipocytes and brown adipose tissue (BAT) have not been studied. In addition, in vitro and ... The results suggest that PRR-mediated inflammation in brown adipocytes may be a potential target for regulating BAT development ... Understanding of the regulation of brown and beige adipocytes will provide novel strategies to reach the goal. Pattern ... activation of PRRs reduces adipogenesis and suppresses expression of brown-specific genes in both classic brown adipocytes and ...https://trace.tennessee.edu/utk_graddiss/3541/
The origin and definition of brite versus white and classical brown adipocytes - Research Collection
Brown adipose tissue; Brown fat; Brite adipocyte; Beige adipocyte; Non-shivering thermogenesis; Bi-directional interconversion ... The origin and definition of brite versus white and classical brown adipocytes. * Mendeley ...https://www.research-collection.ethz.ch/handle/20.500.11850/99732
Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes
This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced ... Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes ... Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes ... Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes ...http://www.recercat.cat/handle/2072/266503
FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation -...
... subcutaneous adipose tissues in terms of macromolecular content and investigate transdifferentiation between white and brown ... FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation ... FTIR imaging of structural changes in visceral and subcutaneous adiposity and brown to white adipocyte transdifferentiation F. ... with a decrease of UCP1 protein content which might be due to the transdifferentiation of brown adipocytes to white adipocytes ...https://pubs.rsc.org/en/content/articlelanding/2015/an/c4an02008a
Poster Barbarloin Regulates Brown Adipocyte Differentiation via Phosphorylation of AMPK | Journal of Nutritional Biology...
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-Gu, Seoul, 02447, Republic of Korea Hye-Lin Kim ...http://www.gratisoa.org/journals/index.php/GJFN/article/view/475
Irisin Stimulates Browning of White Adipocytes Through Mitogen-Activated Protein Kinase p38 MAP Kinase and ERK MAP Kinase...
To investigate the browning effect of r-irisin on adipocytes, we used both 3T3-L1-derived adipocytes and primary rat adipocytes ... Irisin stimulates adipocyte browning via p38/ERK pathways. Primary rat adipocytes and 3T3-L1-derived adipocytes were treated ... White adipocytes store energy (e.g., triglycerides), whereas brown adipocytes consume energy (5,6). Many studies have shown ... Browning effect of r-irisin on rat primary adipocytes. Primary rat adipocytes were treated with r-irisin at indicated ...http://diabetes.diabetesjournals.org/content/63/2/514.full
The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron
... 2013. ... The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role ... The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by ... In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a ...http://ibiss-r.rcub.bg.ac.rs/handle/123456789/1030
Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype | Journal of Translational...
However, the pre-adipocyte cell line SGBS presents a versatile phenotype. A transit expression of classical brown markers such ... Molecular characterization of human brown and white adipocytes, using a myriad of techniques including high-throughput RNA ... showed that PAZ6 and SW872 cells exhibit classical molecular and phenotypic markers of brown and white adipocytes, respectively ... Conversely, white adipocyte markers, including Tcf21, showed reciprocal behavior. Interestingly, leptin levels peaked at day 28 ...https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-015-0480-6
Abscisic acid enhances glucose disposal and induces brown fat activity in adipocytes in vitro and in vivo. | Sigma-Aldrich
Abscisic acid enhances glucose disposal and induces brown fat activity in adipocytes in vitro and in vivo.. [Laura Sturla, ... ABA stimulates glucose uptake by myocytes and pre-adipocytes in vitro and oral ABA improves glycemic control in rats and in ... Here we investigated the role of the ABA/LANCL2 system in the regulation of glucose uptake and metabolism in adipocytes. ... As compared with insulin, ABA treatment of adipocytes induced lower triglyceride accumulation, CO ...https://www.sigmaaldrich.com/catalog/papers/27871880
Brown adipocyte progenitor population is modified in obese and diabetic skeletal muscle. | MRC Centre for Regenerative Medicine
Brown adipocyte progenitor population is modified in obese and diabetic skeletal muscle. Title. Brown adipocyte progenitor ... Brown adipose tissue mitochondria express the unique thermogenic uncoupling protein-1. Recently, brown adipocyte progenitors ... Adipocytes, Brown, Adult, Antigens, CD31, Antigens, CD34, Blood Glucose, Body Mass Index, Cholesterol, Diabetes Mellitus, Type ... The aims of this study were firstly to determine if obesity and diabetes have altered amounts of muscle brown adipocyte ...http://crm.ed.ac.uk/publications/brown-adipocyte-progenitor-population-modified-obese-and-diabetic-skeletal-muscle