Changes in F-actin organization. In order to investigate the effects of exposure to vesicles obtained from size exclusion ... b-d) HCS analysis of fluorescein-phalloidin staining was used to observe changes in actin filaments organization (green) and ... To further characterize the effect of SVEVs obtained from SEC on HUVEC cells, changes in actin organization were investigated. ... Cytotoxicity assays on HUVEC demonstrated the ability of SVEVs in reducing cell viability and changing actin organization. ...

Structure of the F-actin-tropomyosin complex Electron cryomicroscopy reveals the three-dimensional structure of F-actin at a ...

One of their key characteristics is that they recruit actin and actin-binding proteins such as CORONIN1B and WASH which ... Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons ... WDFY2 localizes to actin-stabilized endosome tubules positive for the small GTPase RAB4 and shows a preference for highly ... Depolymerization of actin with Latrunculin caused rapid changes in the morphology of GFP-WDFY2-labeled tubules. We observed ...

Here we show that a minimal reconstituted system consisting of actin filaments, crosslinking molecules and molecular-motor ... is found in a minimal active-gel consisting of actin filaments, molecular-motor filaments and crosslinkers. ... Structure formation and constant reorganization of the actin cytoskeleton are key requirements for the function of living cells ... MacLean-Fletcher, S. & Pollard, T. D. Identification of a factor in conventional muscle actin preparations which inhibits actin ...

... from actin marks older actin filaments for disassembly. Here, the authors show how Pi exits the F-actin interior through a ... The great escape - how inorganic phosphate is released from actin filaments The release of inorganic phosphate (Pi) from actin ... Molecular mechanisms of inorganic-phosphate release from the core and barbed end of actin filaments Release of inorganic ... we show how actin filaments release Pi through a molecular backdoor and demonstrate that this arrangement is distorted in a ...

Structural basis for the target specificity of actin histidine methyltransferase SETD3 *Shaobo Dai ...

Interestingly, mitochondrial association of ADF and n-cofilin during apoptosis was preceded by, and dependent on, actin that ... actin depolymerizing factor), or all ADF/cofilin isoforms to well-characterized apoptosis inducers. Cytochrome c release, ... is a member of the ADF/cofilin family of actin depolymerizing proteins. Recent studies reported a mitochondrial translocation ... STS-induced mitochondrial translocation of n-cofilin and ADF is mediated by actin. (a) Translocation of actin to mitochondria ...

Extended Data Figure 9 Gain and loss of cp110 affects MCC basal bodies, but not the apical actin meshwork.. a, cp110 ... Cilia: Ac-α-Tub (red), Actin: Phalloidin-488 (green) and nuclei: DAPI (blue). g, Expression of cp110, foxj1 and miR-34/449 RNAs ... miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics. Nature Cell Biol. 14, 697-706 (2012) ... Together with the lack of cp110 knockdown to rescue the apical actin meshwork in miR-34/449 morphants, these data indicate an ...

e, Images of day 6 SEMs showing phospho-ezrin, radixin and moesin (ph-ERM; top), podocalyxin (PODXL; bottom) and F-actin. f, ... F-actin (red), nuclei (DAPI, grey). 11-19 s, slicing through the 3D volume showing the inner structure of the multiple ... a, Representative immunofluorescence image of a day 6 SEM showing YS-like, PE-like and VE-like (SOX17) compartments, F-actin ... g, Top, image of a day 6 SEM showing CK7, F-actin and nuclei (DAPI). Bottom, zoom into the multinucleated syncytiotrophoblast- ...

Identification of a novel actin-dependent signal transducing module allows for the targeted degradation of GLI1 *Philipp ...

... β-actin mRNAs as a controls because spinal expression was constant throughout the day. Primer sequences were listed in ... Actin (1:1,000; sc1616-HRP, Santa Cruz Biotechnology) or POL2 (1:1,000; sc900, Santa Cruz Biotechnology). The specificities of ...

Specifically, SNAREs, actin and its associated motor protein myosin shape the vacuole by actin-dependent constrictions. While ... polyclonal rabbit anti-actin antibody (#AS 13 2640, Agrisera, Vännäs, Sweden, raised against Arabidopsis thaliana actin-1/-2/-3 ... Actin-dependent vacuolar occupancy of the cell determines auxin-induced growth repression. Proc. Natl Acad. Sci. USA 113, 452- ... Interestingly, a faint actin signal was observed at the plasma membrane at ≥20 DAP, whereas the signal of tubulin at the plasma ...

7 Subcellular RhoA activity, actin turnover, and LPR distribution in single motile endothelial cells.. a, Montage of actin ... Blanchoin, L., Boujemaa-Paterski, R., Sykes, C. & Plastino, J. Actin dynamics, architecture, and mechanics in cell motility. ... Lifeact: a versatile marker to visualize F-actin. Nat. Methods 5, 605-607 (2008). ... timelapse in a migratory endothelial cell; maximum projection of change in actin structure (Lifeact difference image, obtained ...

Phenylephrine induces elevated RhoA activation and smooth muscle α-actin expression in Pkd2+/− vascular smooth muscle cells * ...

FSCN1-fascin actin-bundling protein 1; and FES-fes proto-oncogene. ...

C3085 and anti-β-actin (AC15) Cat. No. A5441 antibodies were from Sigma-Aldrich. Rabbit monoclonal anti-cleaved caspase 3 ( ... β-actin was used to monitor equal sample loading. ...

FN1, fibronectin; COL1A1, collagen; ACTA2, smooth muscle actin alpha 2. h Functional assays for IPF hallmarks in Ctrl or IPF ... 10d-g). FACTin treatment of IPF hPCLS reduced the levels of the fibrotic markers COL1A1, FN1, smooth muscle actin alpha 2 ( ...

... chicken beta-actin short promoter. ...

Beta-actin was used as loading control. Longer exposition is shown for HepG2 with AXL antibody. (B) Leiomyosarcoma cells were ... and actin (A54441; Sigma-Aldrich) were used, followed by a secondary HRP-linked antibody. Protein bands were detected with ...

Rhodamine phalloidin was used to stain actin fibers within the muscle leg and CellTracker Green was added for AO correction. a) ... 2P-ISIM volume comparison (AO off, AO on + deconvolution) using labeled actin in Drosophila third instar larval brain. See also ... 2P-ISIM volume comparison (AO off, AO on, AO on + deconvolution) using immunolabeled actin in fixed endothelial cells embedded ...

F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins. Nat. Cell Biol. 15, 395-405 (2013). ... Forces that shape cells and tissues can be produced by the contraction of actin filament (F-actin) meshworks by the molecular ... a) F-actin is modelled by polar filaments (red) and motors by springs (green). As motor exerts force on filaments, filaments ... 3b,d). Because ROCK localization depends on its own activity and the actin cytoskeleton37,38,39, the formation of ROCK rings/ ...

Murine beta-actin was used as housekeeping gene (n = 6). d Acute inflammation experimental model workflow (early inflammation ... Beta-actin was used as housekeeping gene. c Immunoblot and quantification of DNMT3A protein expression in human colonic ... Phalloidin (red) and DRAQ5 (purple) was used to visualize actin filaments and nuclei, respectively. Spheroids area (µm2) was ... Beta-actin was used as housekeeping gene. b Gene expression analysis of DNMT3A, CCND1, and CXCL10 in human colonic organoids ...

AFAP1L1 interaction with Nck2 recruits actin-nucleating complexes. Significantly, in osteosarcoma cell lines, knockdown of ... a direct filamentous-actin binding domain and (iii) phospho-tyrosine motifs (pY136 and pY566) that specifically bind Vav2 and ... AFAP1L1 inhibits phosphorylated MLC2 recruitment to filamentous-actin structures, disrupts invadopodia formation, cell ... actin filament-associated protein) family. We show that AFAP1L1 can transform cells, promote migration and co-expression with ...

Human β-actin-forward: 5-CACCAACTGGGACGACAT-3; Human β-actin- reverse: 5-ACAGCCTGGATAGCAACG-3. ... Primers designed to amplify B7-H3 (4Ig and 2Ig isoforms) and β-actin were as follows (Supplementary Table 6). Human B7-H3- ... Reverse transcription-polymerase chain (RT-PCR) reaction of two isoforms and housekeeping gene β-actin in multiple tumor cell ...

c) Vascular smooth muscle cell actin (SMa; yellow) and pericyte marker CD13 in Pdgfrb+/+ control mice illustrating that ...

... anti-actin (1:10,000; Millipore, clone C4), anti-NDUFS3 (1:2000; Abcam, clone 17D95), anti-SDHA (1:1000; GeneTex, GTX101689), ...

Actin was used to demarcate cell boundaries. h, DNA FISH with chromosome paint probes showing Y-chromosome-specific cytoplasmic ...

Actin was used as a loading control. Shown is representative data from three independent experiments. c, NTA of the numbers of ... Actin was used as a loading control. Shown is representative data from three independent experiments. b, Western blot analysis ...

... whereas Cdc42 activates actin-associated proteins, including fascin, formin (mDia2) and Ena/VASP, to induce F-actin bundles in ... which is followed by retraction caused by the depletion of the globular actin pool. Meanwhile, actin polymerization propagates ... also reported V-shaped edge elongation in motile cells16; according to their model, active Rac1 induces actin polymerization ... Chhabra, E. S. & Higgs, H. N. The many faces of actin: matching assembly factors with cellular structures. Nature cell biology ...