We bred PD-1 KO mice with mice carrying the IRES-GFPcre reporter KI at the 3′ untranslated region of the FoxP3 gene (hereafter ... others mutations affecting intronic or noncoding regions that might alter the expression levels are relatively mild (6, 37). ... 5. ex-FoxP3 T cells in autoimmune FoxP3-GFPcreKI/Y PD-1 KO mice. (A) FACS analysis of Tregs, ex-FoxP3 T cells, and CD25+ T ... 5 A and B). In addition, the frequency of GFP+ cells was increased, but these cells may be of a different nature, as many of ...