... on 3T3-L1 cells.. Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid ... Anti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells * Gi-Chang Kim ... Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and ... which regulate adipocyte differentiation in 3T3-L1 cells.. Conclusions : TME may be a potential novel therapeutic agent for the ...
Dive into the research topics of Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line. ... Abundant Active Ingredients of Green Tea Regulate Glucose Metabolism in 3T3-L1 Cell Line. ...
In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form ... Cyclic AMP effects on cell-to-cell junctional membrane permeability during adipocyte differentiation of 3T3-L1 fibroblasts. ... A B Novikoff, P M Novikoff, O M Rosen, C S Rubin; Organelle relationships in cultured 3T3-L1 preadipocytes.. J Cell Biol 1 ... In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form " ...
3T3-L1 cells. 904. 60. 0.8. 1. 2. 3. 14.2. 28.5. 42.8. 0.07. cm2. spot. 17. 33. 50. 1. The response of laser ... Insulin-resistant 3T3-L1 adipocytes and WAT. 635. 5000. 0.072. -. 8. -. 600. daily. for. 10. weeks. The current results ... cell phenotype, cell density, number of cell passages in culture) should be included among eligibility criteria for study ... laser-cell culture surface distance, lid presence during irradiation) and cell-related characteristics (cell type or line, ...
3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. Differentiation of 3T3-L1 ... It is concluded that pro-inflammatory phenotype of differentiated 3T3-L1 adipocyte-like cells, induced by PGF2α is ... PGG enhanced H2O2 production of in PGF2α-treated cells. ... murine fibroblasts into adipocyte-like cells was conducted in ... regulating the metabolism and activity of various cell types and body functions. ...
Cell Culture; Cell Differentiation; Metabolism; Biological Assay; bioassay; In Vitro Techniques; Adipocytes; 3T3-L1 Cells; ... A simple and low-cost bioassay is proposed to quantify glucose consumption in 3T3-L1 adipose cells. Biological Sciences; Cell ... Methods: We worked with 3T3-L1 adipose cells differentiated by 7-8 days, which were exposed to different initial glucose ... Results: The colorimetric assay allowed us to quantify glucose uptake in our cell model, observing a linear response over time ...
3T3-L1 Cells * Adipocytes, White / drug effects * Adipocytes, White / immunology * Adipocytes, White / metabolism* ... 3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. RAW and L1 were then mutually stimulated with ... L1 conditioned media stimulated TNF-α secretion from RAW, and RAW conditioned media increased S100B secretion from L1. The co- ... Results: S100B upregulated TNF-α and M1 markers in RAW, and TNF-α augmented S100B secretion from L1. ...
Sun T, Fu M, Bookout AL, Kliewer SA, Mangelsdorf DJ (2009). "MicroRNA let-7 Regulates 3T3-L1 Adipogenesis". Mol Endocrinol. 23 ... Dröge P, Davey CA (2008). "Do cells let-7 determine stemness?". Cell Stem Cell. 2 (1): 8-9. doi:10.1016/j.stem.2007.12.003. ... "let-7 regulates self renewal and tumorigenicity of breast cancer cells". Cell. 131 (6): 1109-23. doi:10.1016/j.cell.2007.10.054 ... and IL6 links inflammation to cell transformation". Cell. 139 (4): 693-706. doi:10.1016/j.cell.2009.10.014. PMC 2783826. PMID ...
Cell signaling relies extensively on dynamic pools of redox-inactive metal ions such as sodium, potassium, calcium and zinc, ... 3T3-L1 Cells * Animals * Copper / pharmacology* * Cyclic AMP / metabolism* * Cyclic Nucleotide Phosphodiesterases, Type 3 / ... in combination with pharmacological alterations in copper status and imaging studies in a 3T3-L1 white adipocyte model, we ... Cell signaling relies extensively on dynamic pools of redox-inactive metal ions such as sodium, potassium, calcium and zinc, ...
Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 Cell Extracts Inhibit Adipogenesis in 3T3-L1 and HepG2 Cells( ... Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 Cell Extracts Inhibit Adipogenesis in 3T3-L1 and HepG2 Cells ... Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 Cell Extracts Inhibit Adipogenesis in 3T3-L1 and HepG2 Cells ... 3T3-L1 cells treated with a 1:1 ratio of HY7601 and KY1032 during differentiation showed reduced lipid accumulation by Oil Red ...
3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ... 3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ... 3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ... 3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ...
... Inhibitory Effect of Carallia Brachiata Extract Through Regulation of Adipogenesis Pathways in 3T3-L1 Cells / Pages that link ... Inhibitory Effect of Carallia Brachiata Extract Through Regulation of Adipogenesis Pathways in 3T3-L1 Cells. Pharmacognosy ... to Inhibitory Effect of Carallia Brachiata Extract Through Regulation of Adipogenesis Pathways in 3T3-L1 Cells ...
The insulin/Ras pathway of adipocytic differentiation of 3T3 L1 cells: Dissociation between Raf-1 kinase and the MAPK/RSK ...
3T3-L1 Cells, Adipocytes, Adipogenesis, Anilides, Animals, Biphenyl Compounds, Cell Differentiation, Central Nervous System ... Pimozide, a novel fatty acid binding protein 4 inhibitor, promotes adipogenesis of 3T3-L1 cells by activating PPARγ. ... Pimozide, a novel fatty acid binding protein 4 inhibitor, promotes adipogenesis of 3T3-L1 cells by activating PPARγ. ...
It is a lipophilic molecule that rapidly permeates cell membrane. Curcumin has been used as a pharmacological traditional ... 3T3-L1 cells treated with curcumin (0-30 μM) ↓Adipocyte differentiation and fat accumulation. ↓Cell viability. ↓C/EBPβ, PPARγ ... 3T3-L1 cells treated with curcumin (0-30 μM) ↑Apoptosis at 30 μM. ↓Glycerol release. ↓MAPK phosphorylation. 2 and 24 h [121]. ... 3T3-L1 cells treated with curcumin (0-50 μM) for ↑AMPK. PPARγ. ↑Phosphorylation of ACC. ↓Fat accumulation. 8 days. [124]. ...
Reusch, J. E., Colton, L. A., and Klemm, D. J. (2000). CREB activation induces adipogenesis in 3T3-L1 cells. Mol. Cell. Biol. ... Rg3-treated 3T3-L1 cells showed reduced lipid accumulation and total TG levels, and alleviated obesity-induced insulin ... 10 μM ginsenoside Rb1 treatment increased glucose uptake in 3T3-L1 cells and promoted mRNA expression of brown fat marker ... and inhibit lipid accumulation during the differentiation of 3T3-L1 adipogenic cells through AMPK and PPAR-signaling pathways ...
3T3-L1 cell culture and differentiation.. 3T3-L1 preadipocytes were cultured in Dulbeccos modified Eagles medium (DMEM; ... When 3T3-L1 cells were infected with Ad-RAGE, and the cells were induced to differentiate for 6 days, significant numbers of ... or Ad-RAGE-infected 3T3-L1 cells were stained for 1 h with 0.5 μmol/L BODIPY to analyze the proportion of lipid-laden cells ... or Ad-RAGE-infected 3T3-L1 cells were stained for 1 h with 0.5 μmol/L BODIPY to analyze the proportion of lipid-laden cells ...
Western blotting. 3T3-L1 cells were treated as described, and whole-cell extracts were made in RIPA buffer. Equal amounts of ... OLR1 is induced by PPARγ ligands in 3T3 L1 adipocytes. (A) Comparison of OLR1 and aP2 mRNA levels in 3T3-L1 preadipocytes ( ... cells were treated with appropriate reagents (see figure legends) for 48 hours. Total RNA was extracted from 3T3-L1 cells, ... Orlicky, DJ, Schaack, J. Adenovirus transduction of 3T3-L1 cells. J. Lipid Res. 2001. 42:460-466. View this article via: PubMed ...
Co-culturing 3T3L-1 adipocytes and mouse macrophage cells showed incubating adipocytes with IS increased macrophage ... 3T3-L1 cells were seeded in a 96-well plate and allowed to differentiate into adipocytes. After cell differentiation, the cells ... 3T3-L1 cells were seeded on the lower layer and differentiated to adipocytes and mouse macrophage cells (RAW264.7) were then ... OAT expression in differentiated 3T3-L1 adipocytes. Human renal proximal tubular epithelial cells (HK-2 cells) were used as a ...
Parray, H.A., Yun, J.W. Cannabidiol promotes browning in 3T3-L1 adipocytes. Mol Cell Biochem416, 131-139 (2016). ... Parray, H. A., & Yun, J. W. (2016). Cannabidiol promotes browning in 3T3-L1 adipocytes. Molecular and cellular biochemistry, ... Brown fat cells demonstrate higher metabolic activity than white cells. They easily burn off energy as heat, prompting ... Mitochondria are the cells powerhouse. When insulin transports glucose to the cells, the mitochondria transform it into the " ...
... expression in 3T3-L1 cells." in: Journal of cellular biochemistry, Vol. 116, Issue 6, pp. 1153-63, (2015) (PubMed). Zhang, Peng ... PPARG Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum ... PPARG Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum ... PPARG Reactivity: Rabbit Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum ...
Differentiation of 3T3-L1 cells was augmented by MST2 and SAV1 expression and inhibited by knockdown of MST1/2 or SAV1. Title: ... Predicted to be active in cell-cell junction; cytoplasm; and dendrite. Is expressed in several structures, including ... Mammalian ste20-like kinase and SAV1 promote 3T3-L1 adipocyte differentiation by activation of PPARγ. ... oval cells) and eventually develop hepatomas. Title: The Hippo-Salvador pathway restrains hepatic oval cell proliferation, ...
17, 18] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. Inactivation of ... Shu L, Sauter NS, Schulthess FT, Matveyenko AV, Oberholzer J, Maedler K. Transcription factor 7-like 2 regulates beta-cell ... Transcription factor TCF7L2 genetic study in the French population: expression in human beta-cells and adipose tissue and ... it is involved in the development of a wide variety of cell lineages and organs. [16] Potential mechanisms through which TCF7L2 ...
In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. However ... HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes. ECU Author/Contributor (non-ECU co-authors, if there ... HuR binds to a single site on the C/EBP - beta mRNA of 3T3-L1 Adipocytes. http://hdl.handle.net/10342/3315. The described ...
J Cell Mol Med 2015;19:2413 * Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes. Authors: Fernández-Galilea Et al. ... Cell Commun Signal 2012;109:9179 * Protection of protease-activated receptor 2 mediated vasodilatation against angiotensin II- ... Prostaglandin receptor EP3 regulates cell proliferation and migration with impact on survival of endometrial cancer patients. ...
cell line: 3T3-L1. treatment: differentiated TNF. passage: less than 10. Treatment protocol. Before starting any insulin ... 3T3-L1 cells (ATCC) were maintained in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% bovine serum (Invitrogen ... Cells were then incubated in DMEM containing 10% FBS and 10 µg/ml insulin for 2 more days. After Day 4, Cells were maintained ... For the TNFα treatment, cells were treated with 2.5µM of TNFα (R & D Systems) for 24h. For hypoxia treatments, cells were ...
Monosodium glutamate restricts the adipogenic potential of 3T3L1 preadipocytes through mitotic clonal expansion. Cell Biology ... Monosodium glutamate restricts the adipogenic potential of 3T3L1 preadipocytes through mitotic clonal expansion. Cell Biology ... Monosodium glutamate restricts the adipogenic potential of 3T3L1 preadipocytes through mitotic clonal expansion. Cell Biology ... Monosodium glutamate restricts the adipogenic potential of 3T3L1 preadipocytes through mitotic clonal expansion. Cell Biology ...
The cells were selected by FACS. Cell culture and Oil Red O staining HEK-293T and 3T3-L1 cells were cultured in DMEM (Hyclone) ... E) 3T3-L1-Tet-on-shSenp2 cell were treated with or without doxycycline at day 12 of differentiation procedure. The cell lysates ... E) 3T3-L1-Tet-on-shSenp2 cell were treated with or without doxycycline at day 12 of differentiation procedure. The cell lysates ... Plasmid and stable cell line construction To generate stable 3T3-L1-Tet-on-shSenp2 cells, shRNA-targeting Senp2 gene was ...
Current research is focused on the mechanisms that regulate SMAD activity to evoke cell-type-specific and context-dependent ... α as a positive regulator of insulin sensitivity through direct activation of phosphatidylinositol 3-kinase in 3T3-L1 ... A ligand that is secreted by cells and, by spreading to other cells, determines distinct cell fates in a dose-dependent manner. ... Nature Reviews Molecular Cell Biology (Nat Rev Mol Cell Biol) ISSN 1471-0080 (online) ISSN 1471-0072 (print) ...