nih 3t3, 3t3-l1 Cells | Thermo Fisher Scientific - US
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ... Embryonic mouse fibroblast; Properties: contact inhibition; transformation; transfection; cell biology; ability to ...http://www.thermofisher.com/us/en/home/technical-resources/cell-lines/n/cell-lines-detail-334.html
Molecules | Free Full-Text | Modulation of HO-1 by Ferulic Acid Attenuates Adipocyte Differentiation in 3T3-L1 Cells
Therefore, we demonstrated that FA is a positive regulator of HO-1 in 3T3-L1, and may be an effective bioactive compound to ... We investigated whether HO-1 can be activated by FA and suppress adipogenic factors in 3T3-L1. Our results showed that FA ... In addition, HO-1 inhibitor stimulated lipid accumulation, while FA attenuated lipid accumulation in 3T3-L1 treated with HO-1 ... Keywords: Ferulic acid; 3T3-L1; HO-1; adipogenesis; obesity Ferulic acid; 3T3-L1; HO-1; adipogenesis; obesity ...http://www.mdpi.com/1420-3049/22/5/745
Aspirin Breaks the Crosstalk between 3T3-L1 Adipocytes and 4T1 Breast Cancer Cells by Regulating Cytokine Production
Subsequently, culture of 4T1 cells in 3T3-L1 adipocyte-conditioned medium (Ad-CM) and co-culture of 3T3-L1 and 4T1 cells using ... Aspirin inhibited inflammatory MCP-1 adipokine production by 3T3-L1 adipocytes and the cell growth and migration of 4T1 cells. ... α or conditioned medium from RAW264.7 cells. In 4T1 cells, treatment with aspirin decreased cell viability and migration, ... The aim of this study is to investigate the anti-inflammatory and anti-cancer effects of aspirin on 3T3-L1 adipocytes, 4T1 ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147161
IJMS | Free Full-Text | Insulin Induces an Increase in Cytosolic Glucose Levels in 3T3-L1 Cells with Inhibited Glycogen...
Here, we used the fluorescent indicator protein (FLIPglu-600µ) to monitor cytosolic glucose dynamics in mouse 3T3-L1 cells in ... The results show that cells exhibit a low resting cytosolic glucose concentration. However, in cells with inhibited glycogen ... In untreated cells with sensitive glycogen synthase activation, insulin stimulation did not result in a change in the cytosolic ... It has been thought for a long time that glucose entering the cytosol is swiftly phosphorylated in most cell types; hence the ...http://www.mdpi.com/1422-0067/15/10/17827
Molecules | Free Full-Text | Buddleja officinalis Maximowicz Extract Inhibits Lipid Accumulation on Adipocyte Differentiation...
... we elucidated that Buddleja officinalis Maximowicz extract significantly inhibited lipid accumulation during 3T3-L1 adipocyte ... When their adipose tissue morphology was investigated for histochemical staining, the distribution of cell size in the high-fat ... Buddleja officinalis Maximowicz Extract Inhibits Lipid Accumulation on Adipocyte Differentiation in 3T3-L1 Cells and High-Fat ... "Buddleja officinalis Maximowicz Extract Inhibits Lipid Accumulation on Adipocyte Differentiation in 3T3-L1 Cells and High-Fat ...https://www.mdpi.com/1420-3049/17/7/8687
Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as...
This study investigated the changes in the soluble proteome of 3T3-L1 cells upon expression of the WT and the mutant (R316Q) ... Exogenous Expressions of FTO Wild-Type and R316Q Mutant Proteins Caused an Increase in HNRPK Levels in 3T3-L1 Cells as ... Protein extracts prepared from 3T3-L1 cells expressing either the WT or the mutant FTO proteins were used in DIGE experiments. ... This increase may codictate the metabolic changes occurring in the cell and may attribute a significance to HNRNPK in FTO- ...https://www.hindawi.com/journals/bmri/2017/8216180/abs/
Capsaicin and nonivamide similarly modulate outcome measures of mitochondrial energy metabolism in HepG2 and 3T3-L1 cells -...
Capsaicin and nonivamide similarly modulate outcome measures of mitochondrial energy metabolism in HepG2 and 3T3-L1 cells ... Capsaicin and nonivamide similarly modulate outcome measures of mitochondrial energy metabolism in HepG2 and 3T3-L1 cells C. M ... accumulation was reduced to a similar extent after treatment with both test substances during the maturation of 3T3-L1 cells by ... tissue-specific effects of capsaicin and nonivamide on parameters of mitochondrial energy metabolism in 3T3-L1 and HepG2 cells ...http://pubs.rsc.org/en/content/articlelanding/2017/fo/c7fo01626c
AID 744061 - Agonist activity at PPAR-gamma in mouse 3T3-L1 cells assessed as adipocyte differentiation at 1 to 1000 nM after 6...
Agonist activity at PPAR-gamma in mouse 3T3-L1 cells assessed as adipocyte differentiation at 1 to 1000 nM after 6 days by Oil ...https://pubchem.ncbi.nlm.nih.gov/bioassay/744061
The Transcription Factor Fos-Related Antigen 1 Is Induced by Thiazolidinediones During Differentiation of 3T3-L1 Cells |...
B, two days later, confluent 3T3-L1 cells and 3T3-L1 cells treated for 7 days with Dex, Ins, and BRL were treated with ... A, nuclear run-on transcripts were isolated from 3T3-L1 cells 2 days after confluence and from 3T3-L1 cells treated for 7 days ... Induced expression of fra-1 by BRL during differentiation of 3T3-L1 cells. A, differential display analysis of 3T3-L1 cells ... Expression of Fra-1 and JunD proteins during differentiation of 3T3-L1 cells. Nuclear cell extracts were isolated from 3T3-L1 ...http://molpharm.aspetjournals.org/content/59/3/567
Shikonin suppresses ERK 1/2 phosphorylation during the early stages of adipocyte differentiation in 3T3-L1 cells | BMC...
In this study, we investigated the effect of shikonin on adipocyte differentiation and its mechanism of action in 3T3-L1 cells ... Oil Red O staining was performed to determine the lipid accumulation in 3T3-L1 cells. To elucidate the anti-adipogenic ... To investigate the effects of shikonin on adipocyte differentiation, 3T3-L1 cells were induced to differentiate using 3- ... To further confirm that shikonin inhibits adipogenic differentiation through downregulation of ERK 1/2 activity, 3T3-L1 cells ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-207
Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells | Biochemical...
The induction of these two genes was observed only in growth-arrested 3T3-L1 cells, and not in proliferating cells. In NIH-3T3 ... Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells. Makoto ... Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells ... Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells ...http://www.biochemj.org/content/361/3/629
Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells | Korea Science
Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells - Rare Earth Elements;3T3-L1 Cells; ... on proliferation and fatty acids accumulation in 3T3-L1 cell cultures. In Experiment 1, 3T3-L1 preadipocytes in 96-well plates ... confluent 3T3-L1 preadipocytes but inhibits cell proliferation only in preconfluent cells. J. Nutr. 129:602- 606 ... Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells. He, M.L.; Yang, W.Z.; Hidari, H.; ...http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1DMBP_2006_v19n1_119
Gene Expression during 3T3-L1 Cell... | ERA
Gene Expression during 3T3-L1 Cell Differentiation. * Author(s) / Creator(s). *Fu, A. ... a study was undertaken to examine the changes that occur daily in global gene expression as 3T3-L1 cells differentiate from ... Global gene expression patterns spanning 3T3-L1 preadipocyte differentiation. Canadian Journal of Animal Science, 84(3), 367- ... microarray to allow the time-course analysis of the gene expression profile in these differentiating cells. Self-organizing ...https://era.library.ualberta.ca/items/5fca2c9c-40d0-47a3-810d-7d70f2fde7b5
Basal and PGF2α-stimulated secretion of pro-inflammatory cytokines from 3T3-L1 adipocyte-like cells
3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. Differentiation of 3T3-L1 ... It is concluded that pro-inflammatory phenotype of differentiated 3T3-L1 adipocyte-like cells, induced by PGF2α is ... PGG enhanced H2O2 production of in PGF2α-treated cells. ... murine fibroblasts into adipocyte-like cells was conducted in ... regulating the metabolism and activity of various cell types and body functions. ...https://biodiscovery.pensoft.net/article/17757
Changes in protein levels of adipokines in 3T3-L1 cells | Open-i
Changes in protein levels of adipokines in 3T3-L1 cells treated with HCV core protein.(A) The protein level of IL-6 was ... The levels of mRNA are shown as ratios relative to GST treated cells. Mentions: First, we treated mouse 3T3-L1 cells, which ... pone.0131346.g002: Changes in protein levels of adipokines in 3T3-L1 cells treated with HCV core protein.(A) The protein level ... pone.0131346.g002: Changes in protein levels of adipokines in 3T3-L1 cells treated with HCV core protein.(A) The protein level ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4487891_pone.0131346.g002&req=4
Ivy gourd (Coccinia grandis L. Voigt) root suppresses adipocyte differentiation in 3T3-L1 cells | Lipids in Health and Disease ...
Effects of hexane fraction on the expression of lipid metabolism-related genes in 3T3-L1 cells. The cells were cultured in the ... Adipogenesis or the process of fat cell formation in 3T3-L1 cells is known to be sequentially regulated by a network of ... Oil red O staining of the 3T3-L1 adipocytes. The 3T3-L1 adipocytes were washed twice with phosphate buffered saline at pH 7.4 ... the root extract apparently lowered the lipid levels in 3T3-L1 adipocytes. The amounts of accumulated lipid in the 3T3-L1 ...https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-13-88
The Impacts of Testosterone on Insulin Sensitivity in C57BL/6 Mice, 3T3-L1 Adipocytes and HepG2 Liver Cells and Their Related...
The Impacts of Testosterone on Insulin Sensitivity in C57BL/6 Mice, 3T3-L1 Adipocytes and HepG2 Liver Cells and Their Related ... PartⅡThe impacts of testosterone on insulin sensitivity in 3T3-L1 adipocytes and its related molecular mechanismsObjectives:To ... glucose to differentiated 3T3-L1 adipocytes. Phosphorylation and protein expression of insulin receptor（InsR） and its ... PartⅢThe impacts of testosterone on insulin sensitivity in HepG2 liver cells and its related molecular mechanismsObjectives:Our ...https://www.dissertationtopic.net/doc/1553226
Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity - pdf descargar
Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity. . Biblioteca virtual ... This study examined the anti-obesity effect and mechanism of action of blueberry peel extracts BPE in 3T3-L1 cells and high-fat ... Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity - Descarga este documento ... Blueberry Peel Extracts Inhibit Adipogenesis in 3T3-L1 Cells and Reduce High-Fat Diet-Induced Obesity. ...http://libros.duhnnae.com/2017/jun8/149828047433-Blueberry-Peel-Extracts-Inhibit-Adipogenesis-in-3T3-L1-Cells-and-Reduce-High-Fat-Diet-Induced-Obesity.php
Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR VDR GCNF ROR HNF4 LXR LXR ...
... α 0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D h 2d d Induction MediumDifferentiation Medium NR Expression during Adipogenesis of 3T3- ... L1 Cells (2) Fu et al., Mol. Endo. 19: 2437 (2005) Nur77 PPAR γ NUR77 LXR PPAR ... 2 VDR and COUP-TFII Expression during Adipogenesis of 3T3-L1 Cells 0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D VDR COUP-TFII 0 6 12 18 ... 5 Other Nuclear Receptors 0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D 0 2 4 8 18h 1 2 3 4 5 6 7 8 3T3-L1 cells Inducing MediumDjffer. ...http://slideplayer.com/slide/3347478/
Toxicogenomics in the 3T3-L1 cell line, a new approach for screening of obesogenic compounds - Institutional Repository...
Toxicogenomics in the 3T3-L1 cell line, a new approach for screening of obesogenic compounds ...https://repository.uantwerpen.be/link/irua/118061
Molecules | Free Full-Text | Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte...
... the highly galloylated compound 2 was also found to induce potent inhibition of adipocyte differentiation in 3T3-L1 cells. ... Activity-guided isolation of a methanolic extract of Galla Rhois using pancreatic lipase and 3T3-L1 adipocytes led to the ... 3T3-L1; 2-O-digalloyl-1,3,4,6-tetra-O-galloyl-b-D-glucose Galla Rhois; gallotannins; pancreatic lipase; protoaphin-fb; 3T3-L1; ... Pancreatic Lipase Inhibitory Gallotannins from Galla Rhois with Inhibitory Effects on Adipocyte Differentiation in 3T3-L1 Cells ...https://www.mdpi.com/1420-3049/18/9/10629
3T3-L1 Transfection Reagent (CL-173) | Altogen Biosystems: Transfection Reagents for Cell Lines and In Vivo Tissue Delivery
Altogen CRO offers in vivo RNAi services, tumor xenograft models, toxicology testing, stable cell line generation, and cell ... over 100 pre-optimized in vitro transfection kits for cell lines and primary cells, and electroporation delivery products. ... 3T3 Cell Line:. The 3T3-L1 cell line was established in 1974 from a Swiss albino mouse (Mus musculus) embryo. This cell line ... 3T3-L1 cell line undergoes a pre-adipose to adipose-like conversion. 3T3-L1 is an embryonic mouse fibroblast cell line widely ...https://altogen.com/product/3t3-l1-transfection-reagent-emb-fibroblast-cells-cl-173/?full-site=true
Alliin, a Garlic (Allium sativum) Compound, Prevents LPS-Induced Inflammation in 3T3-L1 Adipocytes
... biological response modifiers and the family of cell adhesion-promoting molecules. ... Mouse preadipocyte 3T3-L1 cells are the most commonly studied and available adipogenic cell line. This cell line has been quite ... Cell Culture. 3T3-L1 cells were obtained from American Type Culture Collection (ATCC CL-173) and cultured at 37°C in 5% CO2/95 ... Gene Expression Profile of Alliin Pretreated 3T3-L1 Cells after LPS Stimulus Is Consistent with a Shift in Cell Response to ...https://www.hindawi.com/journals/mi/2013/381815/
Stevioside from Stevia rebaudiana Bertoni Increases Insulin Sensitivity in 3T3-L1 Adipocytes
3T3-L1 cells were cultured and differentiated in 6-well plates. Cells were given TNF-α to induce insulin resistance, as ... Cell Culture and Differentiation. 3T3-L1 preadipocytes were cultured in Dulbecco's Modified Eagle's Media (DMEM). Cells were ... Stevioside was noncytotoxic to 3T3-L1 cells as cell viability was reduced by a maximum of 17%, making it impossible to ... "Effect of 4-nonylphenol on cell proliferation and adipocyte formation in cultures of fully differentiated 3T3-L1 cells," ...https://www.hindawi.com/journals/ecam/2013/938081/
Preparation of cell lysates from 3T3-L1 cells.. 3T3-L1 cells were washed twice with cold PBS and lysed in TNET buffer (1% ... C: Estrogen directly represses Acrp30 levels in 3T3-L1 adipocytes. Mature (day 8) 3T3-L1 adipocytes were incubated for 12 h in ... A: Acrp30 levels in 3T3-L1 adipocytes after overnight E2 exposure. Top panel: Intracellular Acrp30 levels in 3T3-L1 adipocytes ... Cell culture.. 3T3-L1 murine fibroblasts were propagated and differentiated as previously described (15). Dulbecco's modified ...http://diabetes.diabetesjournals.org/content/52/2/268