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*  Suv39h1 Mediates AP-2α-Dependent Inhibition of C/EBPα Expression during Adipogenesis | Molecular and Cellular Biology
E) 3T3-L1 preadipocytes were treated with MSCV vector or MSCV AP-2α. Cell lysates were prepared, and ChIP-qPCR was performed to ... A) 3T3-L1 cells were treated with control or Suv39h shRNA. Knockdown of Suv39h was confirmed by RT-qPCR and Western blotting at ... B) 3T3-L1 preadipocytes were treated with control or AP-2α shRNA. Cell lysates were prepared, and ChIP-qPCR was performed to ... F) MSCV-vector or MSCV-AP-2α-overexpressing 3T3-L1 cells were treated with MSCV-vector or MSCV-HA-C/EBPα. Oil Red O stain was ...
https://mcb.asm.org/content/34/12/2330
*  Caspase Cleavage of Initiation Factor 4E-Binding Protein 1 Yields a Dominant Inhibitor of Cap-Dependent Translation and Reveals...
Cell culture and cytotoxic treatment.Swiss 3T3 cells were treated and maintained as described before (38), and HEK293 cells ... A) Swiss 3T3 cells were treated with etoposide for the times indicated. The samples were analyzed for total eIF4G1 (top) and 4E ... B) Swiss 3T3 cells were treated with staurosporine (St) for 24 h, and extracts were subjected to affinity chromatography on m7 ... To do this, Swiss 3T3 cells were treated with staurosporine and Δ4E-BP1 was copurified with eIF4E on m7GTP-Sepharose and then ...
https://mcb.asm.org/content/22/6/1674
*  Control of Mitochondrial Transcription Specificity Factors (TFB1M and TFB2M) by Nuclear Respiratory Factors (NRF-1 and NRF-2)...
Cell culture and transfections.BALB/3T3 cells used in transfections were maintained in Dulbecco's modified Eagle's medium ( ... In a second system, 3T3-L1 cells were allowed to differentiate into adipocytes, a process that is accompanied by enhanced ... induced during adaptive thermogenesis but is induced upon cell proliferation and down-regulated when cells exit the cell cycle ... Although C2C12 cells normally do not express PGC-1α, its expression is easily detected in infected cells (Fig. 10). Under these ...
https://mcb.asm.org/content/25/4/1354?ijkey=8be96b5cc155c90c379c666286ea2ce500318481&keytype2=tf_ipsecsha
*  Hypoxia-Inducible Factor 1α Induces Fibrosis and Insulin Resistance in White Adipose Tissue | Molecular and Cellular Biology
Fibronectin modulation of cell shape and lipogenic gene expression in 3t3-adipocytes. Cell 35:657-666. ... S17 in the supplemental material). 3T3-L1 cells serve as a model for the differentiation of fibroblast-like preadipocytes to ... Lysyl oxidase: properties, specificity, and biological roles inside and outside of the cell. J. Cell. Biochem. 88:660-672. ... The differentiation of white adipose cells. An electron microscope study. J. Cell Biol. 18:663-679. ...
https://mcb.asm.org/content/29/16/4467.long
*  Table of Contents | Molecular and Cellular Biology
Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA. R Sasada, T Kurokawa, M Iwane, K ... Cell cycle expression of RNA duplex unwindase activity in mammalian cells. R W Wagner, K Nishikura ... The cell-specific elastase I enhancer comprises two domains. F Kruse, C T Komro, C H Michnoff, R J MacDonald ... Yeast STE7, STE11, and STE12 genes are required for expression of cell-type-specific genes. S Fields, D T Chaleff, G F Sprague ...
https://mcb.asm.org/content/8/2
*  Modulation of Transcriptional Regulation by LEF-1 in Response to Wnt-1 Signaling and Association with β-Catenin | Molecular and...
A) NIH 3T3 cells were transfected with an expression plasmid encoding Wnt-1 or CD8-β-catenin fusion protein. Cytosolic cell ... Cell culture, transient transfections, and reporter gene assays.NIH 3T3, HeLa, and Cos7 cells were cultured at 37°C in ... B) Whole-cell lysates from transfected NIH 3T3 cells were prepared, and β-catenin expression was analyzed as described above. ... we used murine NIH 3T3 cells, which contain endogenous β-catenin but lack LEF-1 (68). Cells were transfected transiently with a ...
https://mcb.asm.org/content/18/8/4807?ijkey=82f5b7dfbbf721cde8ec54758e670592580145eb&keytype2=tf_ipsecsha
*  CREB-Binding Protein Acetylates Hematopoietic Transcription Factor GATA-1 at Functionally Important Sites | Molecular and...
C) Whole-cell lysates from transfected NIH 3T3 cells were immunoprecipitated as in panel B. ... At 24 h later, G1E cells (1.5 × 106/flask) were cocultivated with virus-producing BOSC23 cells for 42 h. Infected G1E cells ... The failure to detect acetylated GATA-1 in NIH 3T3 cells in the absence of cotransfected CBP is due to the presence of ... To determine whether CBP can stimulate GATA-1 acetylation in vivo, we cotransfected NIH 3T3 cells with GATA-1 and CBP and then ...
https://mcb.asm.org/content/19/5/3496?ijkey=9517af90c6376297f6f96a7c5af034e60ae85cf1&keytype2=tf_ipsecsha
*  Atypical Protein Kinases Cλ and -ζ Associate with the GTP-Binding Protein Cdc42 and Mediate Stress Fiber Loss | Molecular and...
Cdc42 induces translocation of aPKCλ from the nucleus to the cytosol in NIH 3T3 cells. NIH 3T3 cells were transfected with Flag ... F) The numbers of cells with stress fibers were counted in untransfected cells, V12 Rac-expressing cells, and cells expressing ... COS cells were transfected by the DEAE-dextran method. NIH 3T3 cells and 293 cells were transfected with Lipofectamine (Gibco ... Cdc42 and PKCλ expression in NIH 3T3 fibroblasts reduces the stress fiber content. NIH 3T3 cells were transfected with ...
https://mcb.asm.org/content/20/8/2880?ijkey=94a3f04e323a6bbe79706b007a837af5483e8c50&keytype2=tf_ipsecsha
*  Table of Contents | Molecular and Cellular Biology
Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media. X Zhan, M Goldfarb ... Repair-deficient xeroderma pigmentosum cells made UV light resistant by fusion with X-ray-inactivated Chinese hamster cells. D ... Novel promoter upstream of the human c-myc gene and regulation of c-myc expression in B-cell lymphomas. D L Bentley, M Groudine ... UV light-induced cyclobutane pyrimidine dimers are mutagenic in mammalian cells. M Protić-Sabljić, N Tuteja, P J Munson, J ...
https://mcb.asm.org/content/6/10
*  At Least One Intron Is Required for the Nonsense-Mediated Decay of Triosephosphate Isomerase mRNA: a Possible Link between...
Cell culture, cell transfection, and RNA purification.Mouse Ltk− cells and NIH 3T3 cells were propagated in minimal essential ... NIH 3T3 cell transfections and the purification and analysis of total-cell RNA were as described in the legend to Fig. 1. The ... NIH 3T3 cell transfections and the purification and analysis of total-cell RNA were as described in the legend to Fig. 1. ... NIH 3T3 cell transfections and the purification and analysis of total-cell RNA were as described in the legend to Fig. 1. ...
https://mcb.asm.org/content/18/9/5272
*  Lipid-Overloaded Enlarged Adipocytes Provoke Insulin Resistance Independent of Inflammation | Molecular and Cellular Biology
... it appears that the 3T3-L1 adipocyte has its own merits as a "pure" fat cell model without the influences of other cells found ... Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells. Stem Cell Rev 6:74-85. doi:10.1007/s12015-010- ... Two days after the cells reached confluence (day 0), differentiation of the 3T3-L1 cells was induced in DMEM containing 10% ... Early B cell factor 1 regulates adipocyte morphology and lipolysis in white adipose tissue. Cell Metab 19:981-992. doi:10.1016/ ...
https://mcb.asm.org/content/35/10/1686?ijkey=3aacea3cabb782673847e83682f252d2218f2238&keytype2=tf_ipsecsha
*  In Exponentially Growing Saccharomyces cerevisiae Cells, rRNA Synthesis Is Determined by the Summed RNA Polymerase I Loading...
In NIH 3T3 cells, chemical inhibitors of DNA methylation and histone deacetylation result in an increase in endogenous rRNA ... were active per G1 cell in log-phase NOY88642C cells, while ∼78 to 95 genes (54 to 67% of 143) were active per G1 cell in log- ... the cells most frequently used for these studies are not representative of normally multiplying cells, as are the yeast cells ... Figure 3 shows examples of dispersed nucleoli from two NOY88642C cells (Fig. 3A and B) and from two NOY1051143C cells (Fig. 3C ...
https://mcb.asm.org/content/23/5/1558
*  Oxygen-Dependent Ubiquitination and Degradation of Hypoxia-Inducible Factor Requires Nuclear-Cytoplasmic Trafficking of the von...
Similar data were obtained in other VHL-competent cell lines, including HeLa, NIH 3T3, 293, and Cos-7 cells (data not shown). ... Cell culture, adenoviral infections, drugs, and hypoxia treatment.The 786-0 (VHL−/−) renal clear cell line (i.e., the RCC cells ... VHL−/− RCC cells (cell line 786-0) or the VHL(+) WT-7 stable cell line (expressing HA-tagged VHL) were either not infected (−) ... Cells were also treated with DMOG (1 mM) for 8 h (panels m to r). Cells were stained with an anti-HIF-1α antibody or with ...
https://mcb.asm.org/content/22/15/5319
*  HER2 (neu) Signaling Increases the Rate of Hypoxia-Inducible Factor 1α (HIF-1α) Synthesis: Novel Mechanism for HIF-1-Mediated...
3T3 and 3T3/NEU (NEU) cells were incubated under nonhypoxic or hypoxic conditions, and cell lysates were prepared. Aliquots ... Hypoxia did not induce AKT activity in 3T3 or 3T3/NEU cells, in agreement with previous studies of prostate cancer cells (65). ... The expression of both reporters was increased approximately threefold in 3T3/NEU cells relative to that in 3T3 cells under ... Stable transfectants of mouse NIH 3T3 cells expressing HER2 (21) and parental 3T3 cells were incubated under nonhypoxic and ...
https://mcb.asm.org/content/21/12/3995?ijkey=ab82446b91319f946f0bb94263bab50aee08b661&keytype2=tf_ipsecsha
*  Nerve Growth Factor Activates Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Pathways To...
NIH 3T3 cells and COS cells were maintained under 5% CO2 in DMEM supplemented with 10% calf serum and 10% fetal bovine serum, ... PC12 cells exit the cell cycle and differentiate into sympathetic neuron-like cells (29). The effects of NGF on PC12 cells are ... In addition, p38 MAPK is activated by growth factors such as EGF and FGF in PC12 cells and NIH 3T3 cells. Thus, the ERK/RSK and ... bFGF-treated SK-N-MC cells, and serum-treated NIH 3T3 cells, respectively (14, 66, 76). In this study, we have shown that both ...
https://mcb.asm.org/content/18/4/1946?ijkey=bd6ec37fabbd856453ad8036367896becf5298f4&keytype2=tf_ipsecsha
*  Regulation and expression of a growth arrest-specific gene (gas5) during growth, differentiation, and development. | Molecular...
In Friend leukemia and NIH 3T3 cells, the levels of gas5 gene mRNA were high in saturation density-arrested cells and almost ... Run-on experiments indicated that the gas5 gene is transcribed at the same level in both growing and arrested cells. On the ... These results indicate that in density-arrested cells accumulation of gas5 mRNA is controlled at the posttranscriptional level ... sulfoxide-induced differentiating cells a sharp decrease in the rate of transcription was observed shortly before the cells ...
https://mcb.asm.org/content/12/8/3514?ijkey=3b2c3060bf5f6aa9a78059b85ab4b9759a27c337&keytype2=tf_ipsecsha
*  Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein ...
... and the cells subsequently fused to mouse NIH 3T3 cells to form heterokaryons (33). Before and during fusion, the cells were ... At 24 h posttransfection, cells were incubated with cycloheximide, subsequently fused with mouse NIH 3T3 cells using ... After 24 h, the medium was replaced, and the cells were incubated 4 h later in the presence of 1 × 106 to 2 × 106 mouse NIH 3T3 ... 1995) When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells. RNA 1:453-465. ...
https://mcb.asm.org/content/21/1/209?ijkey=fd1d905b96a7cd76cf9e8c9a1ab7b9730c2b57b2&keytype2=tf_ipsecsha
*  PLEKHG2 Promotes Heterotrimeric G Protein βγ-Stimulated Lymphocyte Migration via Rac and Cdc42 Activation and Actin...
... of the full-length or C-terminally truncated form of PLEKHG2 caused morphological transformation of NIH 3T3 cells, suggesting ... Jurkat T cells (ATCC), HL60 cells (ATTC), and the B lymphocyte cell lines GM19116C and GM18500B (Coriell Cell Repositories) ... Stromal cell-derived factor 1alpha activates LIM kinase 1 and induces cofilin phosphorylation for T-cell chemotaxis. Mol. Cell ... A) Western blotting of PLEKHG2 expression in leukemia cell lines and human T cells. dHL60, differentiated HL60 cells; GMB and ...
https://mcb.asm.org/content/33/21/4294
*  Oxidative and Electrophilic Stresses Activate Nrf2 through Inhibition of Ubiquitination Activity of Keap1 | Molecular and...
Cell culture, transfection, and luciferase reporter analysis.NIH 3T3, 293T, and Cos7 cells were maintained in Dulbecco's ... We transfected Keap1 or Nrf2 expression plasmid into 293T cells, and whole-cell extracts were prepared. Before the whole-cell ... The cell extracts were mixed with extracts of wild-type cells or Nrf2-expressing cells to form the Keap1-Nrf2 complex, followed ... The results of the experiments with cotransfection of Nrf2 and/or Keap1 mutants into NIH 3T3 cells along with the luciferase ...
https://mcb.asm.org/content/26/1/221?ijkey=d7d3bc72b772cbc6b899c96b347f9ebf00a7a9f0&keytype2=tf_ipsecsha
*  Contribution of a TANK-Binding Kinase 1-Interferon (IFN) Regulatory Factor 7 Pathway to IFN-γ-Induced Gene Expression |...
NIH 3T3 cells, or rat oligodendroglial progenitor cells (13, 15, 38).. The IRF7 promoter contains two IFN-γ response regions, a ... It is produced by activated T cells and NK cells and enhances cell-mediated immunity, most prominently by its contribution to ... In vitro natural cell-mediated cytotoxicity against Candida albicans: macrophage precursors as effector cells. J. Immunol. 134: ... A notable exception from this rule is a subset of dendritic cells, plasmacytoid dendritic cells (pDCs), often referred to as ...
https://mcb.asm.org/content/32/6/1032.long
*  N-Terminal Ubiquitination of Extracellular Signal-Regulated Kinase 3 and p21 Directs Their Degradation by the Proteasome |...
A) Parental BALB/c 3T3 (A31) and E1-thermosensitive mutant (ts20) cells were transfected with the indicated ERK3 constructs or ... A) HEK 293 cells were transfected with HA-tagged ERK3Δ or ERK3Δ-0K constructs. After 36 h, the cells were treated for 12 h with ... B) HEK 293 cells were transfected as described for panel A. After 44 h, the cells were pretreated with dimethyl sulfoxide or MG ... Cell culture and transfections.Human embryonic kidney 293 (HEK 293), A31, and ts20 cells were cultured as described previously ...
https://mcb.asm.org/content/24/14/6140?ijkey=828106204c654557b1002e76669f440bd1550ab9&keytype2=tf_ipsecsha
*  Identification and Characterization of Pancreatic Eukaryotic Initiation Factor 2 α-Subunit Kinase, PEK, Involved in...
These results suggest that the kinase may function as a tumor suppressor, since expression of a mutant PKR in NIH 3T3 cells ... 1985) Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. I. ... 1995) Nuclear localization of the interferon-inducible protein kinase PKR in human cells and transfected mouse cells. Exp. Cell ... It is possible that in the transfection studies overexpression of the dominant-negative form of PKR in NIH 3T3 cells interfered ...
https://mcb.asm.org/content/18/12/7499?ijkey=40fdf07f18ea631c83f427a26a272d5044949d5e&keytype2=tf_ipsecsha
*  Phosphorylation and Stabilization of HURP by Aurora-A: Implication of HURP as a Transforming Target of Aurora-A | Molecular and...
A has been found in many tumors and has also been shown to be oncogenic in murine NIH 3T3 or Rat1 cells (5, 54), although no ... Cell culture, cell cycle synchronization, and transient transfection.All cell lines were purchased from the American Type ... The cell cycle stages, as determined by Q-RT-PCR of several markers (p21, PCNA, and cyclin B2) of each cell cycle stage, are ... The expression profiles for Aurora-A and HURP in a HeLa cell cycle panel (A) and in different cell lines and tissues (B) were ...
https://mcb.asm.org/content/25/14/5789?ijkey=205b0f669cbe443aea85a2115694ddfa82fc1a98&keytype2=tf_ipsecsha
*  FRS2 Proteins Recruit Intracellular Signaling Pathways by Binding to Diverse Targets on Fibroblast Growth Factor and Nerve...
To ascertain the interaction between FRS2α and the FGF receptor, we used three cell lines (Fig.1): NIH 3T3 cells which express ... we used parental PC12 cells and PC12 cells overexpressing both TrkA and FRS2α (PC12-Trk-FRS2α). Quiescent PC12 cells were ... B) NIH 3T3 (mouse fibroblast), L6 (rat myoblast), and PC12 (rat pheochromocytoma) cells stably transfected with FGFR1 were ... Human 293 cells were cultured in the same medium. PC12 cells stably expressing TrkA and FRS2α (PC12-TrkA-FRS2α) or FGFR1 and ...
https://mcb.asm.org/content/20/3/979?ijkey=83748833d6a9bbede69449e685ae7e3e484d000f&keytype2=tf_ipsecsha
*  Stage-Specific Repression by the EKLF Transcriptional Activator | Molecular and Cellular Biology
... in NIH 3T3 cells as a non-erythroid cell type. The results show that, unlike the erythroid K562 line, GAL/EKLF does not repress ... Second, K562 cells are human and MEL cells are murine. To remove these issues from consideration, we investigated the HOX11- ... Cell lines, plasmids, and antibodies.The cell lines, plasmids, and antibodies used in this study were previously described (13 ... Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. Annu. Rev. Cell Dev. Biol. 17: ...
https://mcb.asm.org/content/24/23/10416?ijkey=795c55d79e01770fb3517b5b1eb024107cf8174b&keytype2=tf_ipsecsha