p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerass or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and P. olevorans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.
TY - JOUR. T1 - Development of a DNA microarray chip for the identification of sludge bacteria using an unsequenced random genomic DNA hybridization method. AU - Byoung, Chan Kim. AU - Ji, Hyun Park. AU - Man, Bock Gu. PY - 2004/12/15. Y1 - 2004/12/15. N2 - A tool, based upon the DNA microarray chip, for the identification of specific bacteria from activated sludge, using the hybridization of genomic DNA with random probes, is described. This chip was developed using the genomic DNAs from Gordonia amarae, the natural filamentous actinomycete that causes sludge foaming and bulking, as well as a nonfilamentous floc forming bacterium (Zoogloea ramigera) and the skin pathogen Mycobacterium peregrinum without any sequence information. The sets of target probes on amine-coated glass were made from a genomic library, constructed with PCR products derived from randomly fragmented genomic DNAs extracted from pure cultures of the three strains. Initial hybridization results, when pure cultures were ...
When I arrived home, I dumped the bacteria culture into the crock along with two bottles of wine. Over a period of three months I watched the wine transform into vinegar. The transformation began when a hazy veil covered the liquid. The veil is referred to as bacteria zoogloea. Undisturbed and relying on oxygen, the zoogloea forms on the surface of the vinegar to protect the contents underneath. As it grows it becomes leather-like but upon touching it you discover a slimy, slippery yet solid rubbery gelatinous mass - like raw liver. If left undisturbed it will keep growing and could eventually take over the entire container. Allowing alcohol additions to wet the surface of the zoogloea cuts off oxygen. It will sink to the bottom lifeless allowing another to form to protect the contents (description partially taken from Brian J. Helsaples A - Z vinegar glossary which is no longer online).. Mother of Vinegar is defined as a slimy, gummy substance made up of various bacteria - specifically ...
When I arrived home, I dumped the bacteria culture into the crock along with two bottles of wine. Over a period of three months I watched the wine transform into vinegar. The transformation began when a hazy veil covered the liquid. The veil is referred to as bacteria zoogloea. Undisturbed and relying on oxygen, the zoogloea forms on the surface of the vinegar to protect the contents underneath. As it grows it becomes leather-like but upon touching it you discover a slimy, slippery yet solid rubbery gelatinous mass - like raw liver. If left undisturbed it will keep growing and could eventually take over the entire container. Allowing alcohol additions to wet the surface of the zoogloea cuts off oxygen. It will sink to the bottom lifeless allowing another to form to protect the contents (description partially taken from Brian J. Helsaples A - Z vinegar glossary which is no longer online).. Mother of Vinegar is defined as a slimy, gummy substance made up of various bacteria - specifically ...
Due to the growing need to find a more efficient and environmentally friendly alternative to conventional waste removal methods, industries are paying more attention to the function of bacteria and their EPSs in bioremediation.[14]. Researchers found that adding EPSs from cyanobacteria to wastewaters removes heavy metals such as copper, cadmium and lead.[14] EPSs alone can physically interact with these heavy metals and take them in through biosorption.[14] The efficiency of removal can be optimized by treating the EPSs with different acids or bases first before adding them to the wastewaters.[14] Contaminated soils contain high levels of Polycyclic aromatic hydrocarbons (PAHs); EPSs from two bacteria, Zoogloea sp. and Aspergillus niger, are efficient at removing these toxic compounds.[15] EPSs contain enzymes such as oxidoreductase and hydrolase, which are capable of degrading PAHs.[15] The amount of PAHs degradation depends on the concentration of EPSs added to the soil. This method proves to ...
Cyanobacteria are diverse in their habitats, structure, and metabolism that can grow as unicellular or long filaments and secreting high molecular mass polymers (extracellular polymer substances, EPS), which can either be released into the surrounding environment or remain attached to the cell surface. Four different cyanobacterial environmental samples (soil crust, microbial mats, pond water and marine bloom) were studied to compare between the cyanobacteria species that grow and form cyanomatrix and cyanofilms in those environmental habitats using light and scanning electron microscope (SEM). Diatoms were also detected with cyanobacteria at microbial mats and pond water samples. All samples had variable concentrations and compositions of extracellular matrix and biofilm. Microscopic observation and analyses also revealed complex network of filamentous cyanobacteria and extracellular polymer secretions, which binds and traps particles of soil and minerals ...
Book Dave Dugan for your next event, a one-of-a-kind delivery accompanied by impeccable timing and imaginative comedy will leave your guests wanting more!
When biofilms (aggregations of bacteria and extracellular polymer secretions) in samples from the Carter Sandstone of Alabama were prepared for scanning electron microscopy (SEM) using different dehydration techniques, the organic material had visibly different textures and distributions. In order to assess whether the variation was attributable to sample preparation or to inherent biofilm heterogeneity, each of five techniques were tested 3 to 10 times on small (1 cm) pieces of the Carter Sandstone containing either a strain of bacteria cultured from and reintroduced into the rock, or an in situ biofilm grown by injection of nutrients through core samples. The techniques tested were (1) air drying alone; (2) fixation in 10% glutaraldehyde with air drying; (3) ethanol dehydration with hexamethyldisilazane (HMDS) drying [2.5% glutaraldehyde, ethanol dehydration, and HMDS]; (4) ethanol dehydration with critical-point drying; and (5) ethanol and acetone dehydration with critical-point drying. ...
Brain Donors is a game attempt to redo the Marx Brothers A Night at the Opera in a contemporary setting. John Turturro plays the Groucho
Dragons coach Steve Price said he would speculate as to if Dugan would miss any game time from the injury, saying the club will wait until the results of scans before commentating more.. Josh has been put in a brace and will undergo scans on Monday, Price said.. We will not have any further updates until we receive the scan results so it is too early at this stage to address any expected return dates.. ...
St George Illawarra Dragons fullback Josh Dugan believes the individual form of his NSW Blues teammates is irrelevant ahead of the second State of Origin fixture against the Queensland Maroons at the Melbourne Cricket Ground on Wednesday, June 17.
CRONULLA star Valentine Holmes insists he is ready to fight off any plans new recruits Josh Dugan and Matt Moylan may have to steal his beloved fullback jumper ...
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Relationship between transport of bacteria and their clogging efficiency in sand columns. Effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns
Chapter 1 of this hyptertextbook presented a general introduction to biofilms. We will begin this chapter by presenting a brief review emphasizing biofilm processes which are of fundamental significance and also important to biotechnology applications.. Figure 1 shows that microbial cells are initially present in aqueous solution in suspended or planktonic state. If these cells find their way near a surface they have a very strong tendency to adhere to the surface. If environmental conditions in the aqueous fluid are favorable for growth (i.e. sufficient substrate and growth nutrients) then the attached cells will grow, divide and form new cells along with matrix of extracellular polymer (EPS) which binds the cells to each other and to the surface (substratum). The aggregate of cells and EPS, together with any trapped inert particles and organic matter is termed the biofilm. Products of cell metabolism and biotransformation are released back into the aqueous phase along with cells which ...
Exploring life within the biofilm and how free-living microbes thrive, and focus on life history patterns of symbionts. Finally, big dairy (Parmalat- South Africa) was found guilty of repeatedly hiding serious infections (Salmonella and Listeria,)from their consumers. General microbiome Biofilm formation enables free-living nitrogen-fixing rhizobacteria to fix nitrogen under aerobic conditions. Wang D. ISME Journal…
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what does it mean when your wbc is high normal is 4, 600-10, 000 mine is 11, 500? Answered by Dr. Michael Dugan: Slight WBC: Elevation is seen in infection, stress of another illness,...
STARTUPS TO START NOTICING: SIP CITY BRIGHTESTYOUNGTHINGS | Kaylee Dugan Maybe youre sick of kombucha. Maybe youve tried out every possible brand and flavor at Whole Foods and youre tired of fighting your way through the tech bros and fitness influencers to the display case. Or maybe youve been making it at home. Y
Bacterial strain CCBAU 25048T was isolated from root nodules of Kummerowia stipulacea grown in Shandong province of China. Cells of the strain were Gram-negative, strictly aerobic, non-spore-forming, motile short rods. Phylogeny of 16S rRNA gene sequences revealed that the strain belonged to the genus Shinella, a member of family Rhizobiaceae. Its closest phylogenetic relatives were Shinella granuli Ch06T and Shinella zoogloeoides IAM 12669T, respectively showing 98.3 and 98.9 % 16S rRNA gene sequence similarity. Strain CCBAU 25048T had DNA-DNA relatedness of 43.5 and 34.8 %, respectively, with S. zoogloeoides JCM 20728T and S. granuli JCM 13254T. In addition, in TP-RAPD analysis, different patterns were obtained for these three strains and some rhizobial strains. The nifH, nodC and nodD sequences of CCBAU 25048T were identical or very similar to those of bean-nodulating Rhizobium tropici strains. Several phenotypic characteristics, including the use of citrate and d-ribose as carbon sources and growth
On a sunny January morning, shortly after Great Notion Brewing opened the doors to its Portland, Oregon, taproom for the first time, it had a visitor. He was an employee from a brewery a few miles away who had come to sample the newcomers wares, and he chose a pint of RIPE, a 7% American IPA. But when Great Notion co-founder and brewer James Dugan set the beer before him, the visitor seemed incredulous. He lifted the glass high in the air, squinting at it. I just want you to know, the man told Dugan, This style of beer is not going to fly in Portland. He went on to tell Dugan that Great Notion needed to work on its filtration or no one would be willing to buy its ales.. The problem? The IPA Dugan poured for him was cloudy.. Now, cloudiness absolutely can be a sign of a low-quality beer. If there are issues with yeast and its fermentation, certain adjuncts or, yes, filtration, tiny particles remain suspended in a beer, murking it up and contributing off-putting flavors. But, Dugan says, ...
Notice: This opinion is subject to correction before publication in the Pacific Reporter. Readers are requested to bring errors to the attention of the Clerk of the Appellate Courts, 303 K Street, Anchorage, Alaska 99501, phone (907) 264-0608, fax (907) 264-0878, e-mail [email protected]. THE SUPREME COURT OF THE STATE OF ALASKA MICHAEL R. DUGAN, ) ) Supreme Court No. S-11133 Appellant, ) ) Superior Court No. v. ) 3AN-01-33545 CI ) ATLANTA CASUALTY ) COMPANIES, ) O P I N I O N ) Appellee. ) [No. 5900 - June 3, 2005] ) Appeal from the Superior Court of the State of Alaska, Third Judicial District, Anchorage, Mark Rindner, Judge, and John Suddock, Judge. Appearances: Jeffrey J. Barber and Steve Sims, Law Offices of Steve Sims, Anchorage, for Appellant. James B. Wright, Jerald L. Marcey and James B. Wright & Associates, and Rebecca Hozubin, Wilkerson & Associates, Anchorage, for Appellee. Before: Bryner, Chief Justice, Matthews, Eastaugh, Fabe, and Carpeneti, Justices. ...
Jeff Pierson, orthopedic surgeon and medical director for the St. Vincent Center for Joint Replacement, is featured on the cover of the current issue of Indianapolis Monthly. Dr. Pierson, a 1982 graduate of DePauw University, is among the Top Docs listed inside the magazine and is the subject of a full-page profile, as is his DePauw classmate, Thomas Dugan, president of Cancer Care Group.
Available in: DVD. Max Dugan (Jason Robards Jr.) is an elderly neer-do-well whose tenuous mob connections have made him persona non grata with his
LAS VEGAS, NEVADA: As the legendary five-time Grammy-winning punk rock trio Green Day filled out a project studio in anticipation of recording its twelfth studio album, their longtime engineer, Chris Dugan (U2, Iggy Pop, Smash Mouth), collaborated on the equipment choices. They filled it with their favorites, including API, BAE, Manley, Vintech, Universal Audio, Chandler […]
Wiesenthal,Tom Dugan, director Jenny Sullivan, Holocaust, hope, tolerance, universality, video interviews, Oct. 24th, Acorn Theatre-Oct.24-Feb.1
Dr. James Dugan MD practices as a Urologist provider at 8091 Township Line Rd in Indianapolis, Indiana. View directions, phone number, accepted insurance, procedures, Medicare costs, new patient status, language support, ratings, and NPI.
Blues centre Josh Dugan got through a second consecutive full training session unscathed and is confident hell be right to play, but will know on Saturday.
The microfiltration characteristics of Bacillus subtilis broths under different culture conditions were studied. In the no medium addition culture condition the major component in the broth was sole B. subtilis cells. The filtration curve of dt/dv vs. v (reciprocal of filtration flux vs. filtrate volume) can be divided into three regions. After a short relaxation time the tangent slope increases drastically. This implies that significant cell deformation and cake compression occurs in the second region. The average specific cake filtration resistance will reach the maximum value at the filtration curve inflection point. The cake compressibility gradually decreases in the third region because most solid compressive pressures are depleted by the formed compact cake. In the medium addition culture condition the B. subtilis cells and extracellular polymer substances (EPS) flocs concentrations in the broth increase markedly with culture time. The filtration curve tangent slope increases continuously ...
One of the most important resistance to antibiotics mechanism displayed by pathogens microorganisms is the growth in biofilms. The biofilm matrix, may act as a barrier limiting the diffusion of antimicrobials, favouring the enzymatic inactivation of antibiotics, or its binding to extracellular polymers. It is currently estimated that more than 60% of the bacterial infections in humans imply the formation of biofilms, such as those from P. aeruginosa mucoid strains in the lung of cystic fibrosis patients. The ultra-small (, 200 nm diameter) solid lipid nanoparticles (USNA) are highly stable against heat, oxidation, and hydrolytic attacks. Their highly negative Z potential together with an unusual small size are optimal to slide into the biofilm cavities. USNA are currently being tested to improve the delivery of the antibiotic colistin on the biofilm produced by a mucosa strain of P. aeruginosa. ...
Side effects from Dabigotran range from gastrointestinal irritation to life-threatening and even fatal hemorrhaging. Usually, this bleeding takes place in the GI tract, which can result in bloody stools as well as vomiting. However, patients have also experienced dark and bloody urine, bleeding gums and excessive menstrual flow as well as bruises that do not heal - and even get worse. In the most serious cases, cerebral hemorrhaging (bleeding in the brain) can occur.. Warfarin is also known to cause excessive bleeding. However, according to studies published in a number of medical journals, this can be mitigated by treating the patient with intravenous doses of Vitamin K, fresh frozen plasma and/or prothrombin complex concentrate (PCC), a combination of substances that promote blood clotting. These treatments do not appear to help in the case of hemorrhaging due to dabigotran.. In addition to risks associated with hemorrhaging, a study published in the January 9, 2012 issue of Archives of ...
For The Love Of Dogs Unforgettable, are the sounds of someone heaving their guts out, stomach contents splattering on the bare floor, and the sounds from the observers nearby who own the floor. In this particular incident, the observers were my sister and her husband, and the someone was Dugan, my 130 lb, 8-month old Irish Wolfhound.. We had gone to a holiday Christmas party around noon, and we had left my two dogs at my sisters house with her dog. I suggested taking my two dogs in my minivan so I could keep an eye on them, but my sister reassured me that the three dogs would be fine. She was wrong. When we arrived home around 5:30, there was obvious destruction. Mostly it was destruction of dog toys and a pair of Jims shoes. Evidence was strewn all over the living room, dining room and kitchen. We could only wonder how much of the evidence had been eaten. All seemed well with Dugan until dinnertime when he wasnt interested in eating his food. He has always been interested in his food. It ...
Explore how modern day B2B marketing is both art and science, and the challenges in balancing brand building with performance marketing.
It was susequently reported at the following Annual General Meeting on 8th May 1909 that the Council had entered into negotiations with the tenants adjoining the 9 hole course, and succeeded in arranging with Mr. James Dugan and Mr. Robert Dugan for sufficient ground to make an additional nine holes at an increased rent of £24 and £18-10s respectively for a thirty-eight years lease. Sir H.J.L. Bruce, who took a keen interest and gave great assistance in all the proceedings connected with the extension, extended the lease of the ground held under him for a like period at the nominal rent of 5s per year for which the Club tendered its heartiest thanks.. The Formal Opening of the New Eighteen Hole Course was carried out by Sir Hervey and Lady Bruce on 15th July 1909 when the former drove the first ball from the first tee, in the presence of a large number of ladies and gentlemen, and declared the new course open for play ...
PtJ ./s, 1/ () ~ f!..1t1L ~; ioye,iti ·fioretti MARIAN COLLEGE Indianapolis, Indiana LITERARY ANTHOLOGY VOLUME 42 NO. / /983 - ,rtf THE LIGHTS OF GODSEND/ Deb Sears DUSTY SANDALS/ Lisa Bernhard 15 YOUVE GOT ITl USE IT II Richard Peak 1.6 THE POEM IN ROOM 2(b/ Kent Daniels 21 MR. JOHN/ Lauren Emst 22 AMERICAN HIGHWAY/ Hichae 1 Dugan 28 HAIK.U/ Drew Appleby 29 ONE THAT GOT AWAY/ Terence Hanley 30 ARRP1AL AT GRANNYS/ Deb Sears 33 HAPPy BIRTHDAY--2oo3/ Audrey Pearson 35 PROFESSOR OF CHEMISTRY/ Deb Sears 39 5:00 A.M./ Thomas OBrien 40 COVER/ Jill Thompson pp. )~, ~, 38/ Ellen Petrai1iB p. 21/ Jeanne Byrnes LETTERING/ Sister Stella 2if Ill!!&-- Debbie Sears Kent Daniels Sister Stella Debbie Sears Michae 1 Dugan Terence Hanley c!lijjdp~ Sister H. Stella Gampfer, OSF 1( 675 0 · - .... ~. \~. ,central plains edge of a city: Godsend anno Domini 2375 ••. She stood before the threshold stone, trembling with cold and fatigue. Dry grass and dead cornstalks rustled in the garden behind her; a ...
Hanks gained 30 pounds to play baseball coach Jimmy Dugan in A League of Their Own and dropped from 225 pounds to 170 for Castaway.
Dillon JJ, DeSimone CV, Sapir Y, Somers VK, Dugan JL, Bruce CJ, Ackerman MJ, Asirvatham SJ, Striemer BL, Bukartyk J, Scott CG, Bennet KE, Mikell SB, Ladewig DJ, Gilles EJ, Geva A, Sadot D, Friedman PA. Noninvasive potassium determination using a mathematically processed ECG: proof of concept for a novel blood-less, blood test. J Electrocardiol. 2015 Jan-Feb; 48: (1)12-8 ...
McDonald CJ, Blevins L, Dexter P, Schadow G, Hook J, Abernathy G, Dugan T, Martin A, Phillips R, Davis M. Demonstration of the Indianapolis SPIN Query Tool for De-identified Access to Content of the Indiana Network for Patient Cares (a Real RHIO) Database [Demo] AMIA Annu Symp Proc. 2006:1194 ...
McDonald CJ, Blevins L, Dexter P, Schadow G, Hook J, Abernathy G, Dugan T, Martin A, Phillips R, Davis M. Demonstration of the Indianapolis SPIN Query Tool for De-identified Access to Content of the Indiana Network for Patient Cares (a Real RHIO) Database [Demo] AMIA Annu Symp Proc. 2006:1194 ...
Lynn Dugan prides herself as a registered dietitian, guiding people toward nutritious meals and leading them to healthier, active lifestyles. Yet this Glen Ellyn mom has her own challenge: finding the time to feed her own busy family. I know how hard it is to feed active kids a healthy diet, she said. If I have trouble with my own family, I cant imagine how other people would struggle in their own kitchens.
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Kruglanski, Arie; McCauley, Clark; Ellis, Devin; Dugan, Laura; Boyd, Marcus A.; Wilson, Margaret; Jensen, Michael; Asal, Victor ...
Dr. Dugan responded: Skin lesions are. Very difficult to assess without a visual. If the bumps are serious have your doctor evaluate them and help to make a plan.
Al Pacino romantically pursuing a cross-dressing Adam Sandler around a medieval castle should be stunningly surreal, so its a not-inconsiderable failure that Jack and Jill manages only dim, desperate outrageousness instead. Credit some of that misfire to director Dennis Dugan (I Now Pronounce You Chuck & Larry, Happy Gilmore), whose...
THE Raiders will field a full-strength side for the must-win clash against the Sharks with both Josh Dugan and Sam Williams passing searching fitness tests yesterday.
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You should get a Nerd in your life! And if you have one, be nice to him or her. Regina Dugan, recently the head of the Defense Advanced Research Projects Agency (DARPA) suggested if we actively sought out the Nerd in ourselves we may evolve a greater understanding of the amazing possibilities within each of us.. What would you attempt to do if you knew you could not fail? This is a question we adults are advised to ask ourselves at least once a week by leadership and motivation experts. And it is a good question to pose to all the young people exploring their freedom this summer. Sports camps, science camps, space camp, swimming camp. Each of us needs time for renewal. It is also a way to work harder and stretch just a bit beyond your comfort zone.. I embraced the Nerd in myself years ago, probably in the early eighties. I had to. There were no typesetting computers in Las Cruces so I had to buy one for my business. Then I had to fix it, because there were no repair people here either. It is not ...
No. 10: Geena Davis is the star player in A League of Their Own, but Tom Hanks steals a number of scenes as drunken manager Jimmy Dugan.
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