In this study, phosphate-rich supernatant at the end of anaerobic phase was extracted by a certain side-stream ratio for chemical precipitation to investigate the optimal conditions for phosphorus recovery. The effect of side-stream reaction on the performance of the mainstream Enhanced Biological Phosphorus Removal (EBPR) system was also explored. The experiment was carried out in a sequencing batch reactor (SBR) operated in an alternating anaerobic/aerobic mode with dissolved oxygen controlled at 1.0 mg · L−1. The results showed that the optimum magnesium source,temperature, stirring speed and reaction equilibrium time for side-stream phosphorus recovery were: MgCl2 · 6H2O, 25 °C, 150 rpm and 20 min, respectively. It was also observed that the average phosphorus removal efficiency of the mainstream system maintained as high as 90.7% during the side-stream extraction period despite insufficient time for phosphate uptake under limited dissolved oxygen condition and phosphate deprivation of ...
A side-stream respiration monitoring sensor includes a body having a first end, a second end, and a detecting section disposed between the respective ends. The detecting section includes a first port and a second port positioned on generally opposite sides of a restricting member. The restricting member extends into a flow path formed through the body such that a pressure differential is generated between the first port and the second port. A sampling port is positioned downstream relative to a patient from the first and the second port and configured to acquire a respiration sample. The sensor is constructed to monitor respiration performance of premature infants
The overlying stratum corneum is para- keratotic. Not more than 0. 0 пReference ciprр (a). The ap1colateral cell JUnctiOn consists of a zonula occludens (ZO) (t1ght junction).
TJP2兔多克隆抗体(ab81679)可与人样本反应并经WB, ELISA实验严格验证并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
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Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR, Sekaran Muniandy, Koon Chu Yaiw, Jack Bee Chook,
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Length of an elongated belt-like cloth receivable in a treatment tank is increased by a compact and simple mechanism, and, moreover, impact force applied to the cloth is enhanced, whereby efficiency in cloth treatment such as washing in water, scouring, dyeing and treatment quality are improved. A treatment tank 10 used in carrying out the invention is divided into a front chamber 12 and a rear chamber 14, and a cylindrical rotary member 28 comprising a plurality of rods 30 arranged to form a cylinder is disposed on the midway of a passage 26 communicating the two chambers. A predetermined length of the cloth 20 is fed into the treatment tank, and the cylindrical rotary member is rotated alternately in forward and reverse directions, whereby conveyance of the cloth is repeated between the front and rear chambers, and during such repetition of conveyance, a required treatment is applied to the cloth.
Human TJP1 partial ORF (ACE87388.1, 338 a.a. - 638 a.a.) recombinant protein with GST tag at N-terminal. (H00007082-Q01) - Products - Abnova
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0011]The tight junctions form a selective barrier regulating the passage of ions and molecules through paracellular space. Under electron microscopy, it is noted that the tight junctions form a series of fusion points between the outer leaflets of the plasma membranes of two adjacent cells. These membrane contact zones appear, by freeze fracture, in the form of a continuous network that surrounds the apex of each cell. This network is constituted by membrane protein polymers, the claudins and occludin which are themselves connected to cytoplasmic proteins among which in particular the zonula occludens proteins (ZO-1 to 3) are to be found. Occludin, the claudins as well as the ZO-1s are often the target for bacterial toxins, leading to an alteration in the organisation and the function of the tight junctions (Coraux et al., Am J Respir Cell Mol Biol 2004, 5:605-612 ...
A high molecular weight tight junction-associated protein, ZO-1, has been demonstrated in liver (hepatocytes) and in both epithelium and endothelium. We carried out studies to examine the presence of the protein in vascular endothelial cell cultures and several other types of cultured cells, and the relationship between the ZO-1 protein content and confluency of endothelial cell monolayers. Immunofluorescence labelling of endothelial monolayers and two types of epithelial monolayers, IEC-6 and MDCK, with monoclonal antibody against ZO-1 protein localized the protein to the cell peripheries. Its association with the cell periphery only occurred when cells had contact with one another as demonstrated in endothelial cells. We have been able to show a positive correlation between the ZO-1 content of the cells and the extent of monolayer confluency in the endothelial cells by immunoblotting. The protein is much less expressed in nonconfluent endothelial cell monolayers and totally absent from mouse ...
Tight junction-associated protein 1 is a protein that in humans is encoded by the TJAP1 gene. TJAP1 has been shown to interact with DLG1. GRCh38: Ensembl release 89: ENSG00000137221 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000012296 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Kawabe H, Nakanishi H, Asada M, Fukuhara A, Morimoto K, Takeuchi M, Takai Y (Dec 2001). "Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells". J Biol Chem. 276 (51): 48350-5. doi:10.1074/jbc.M107335200. PMID 11602598. "Entrez Gene: TJAP1 tight junction associated protein 1 (peripheral)". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1-2): 171-4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5-end-enriched cDNA library". ...
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The TJP2 protein (Tight Junction Protein 2, sometimes called ZO2) plays a role in "tight junctions". Tight junctions are areas where the membranes of two adjacent cells join to form a barrier. The barrier controls what molecules are able to pass between cells. Such junctions are important throughout the body, and TJP2 is not specific to the liver. A mild form of liver disease associated with mutations in the TPJ 2 gene was previously called familial hypercholanaemia (which means high bile salts in blood). Only a small number of patients with PFIC caused by TJP2 mutation have been studied so far, so it is not yet understood of what manifestations, other than liver disease and its consequences that TJP2 deficiency patients may have.. ...
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This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA…
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Meerdere linken in de vroegere artikelen zullen vermoedelijk niet werken, maar u kan de titel dan kopiëren en plakken in het "Zoek" venster om zo bij het juiste artikel te komen. Ik maak ere wrek van om de artikelen vanaf 2005 terug te editen en daarin de linken te vernieuwen zodat zij terug zullen werken. Maar dit vraagt enige tijd. Daarom verzoek ik hiervoor begrip teh hebben indien u een link tegen komt die niet meer werkt ...
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TY - JOUR. T1 - The tight junction protein ZO-2 localizes to the nucleus and interacts with the heterogeneous nuclear ribonucleoprotein scaffold attachment factor-B. AU - Traweger, Andreas. AU - Fuchs, Renate. AU - Krizbai, I.. AU - Weiger, Thomas M.. AU - Bauer, Hans Christian. AU - Bauer, Hannelore. PY - 2003/1/24. Y1 - 2003/1/24. N2 - Zonula occludens proteins (ZOPs), currently comprising ZO-1, ZO-2, and ZO-3, belong to the family of membrane-associated guanylate kinase homologue (MAGUK) proteins that are involved in the organization of epithelial and endothelial intercellular junctions. ZOPs bind to the cytoplasmic C termini of junctional transmembrane proteins linking them to the actin cytoskeleton. They are characterized by several conserved modules, including three PDZ domains, one SH3 domain, and a guanylate kinase-like domain, elements indicating that ZOPs may serve multiple purposes. Interestingly, ZOPs contain some unique motifs not shared by other MAGUK family members, including ...
Connexin. 43 (Cx43) is the predominant gap junction (GJ) protein in the mammalian ventricular myocardium. The precise spatial order of Cx43 GJ channels in the heart is thought vital for maintaining cardiac synchrony. Disruption or remodelng of this order is a hallmark of arrhythmic disease and has been reported in ischemic and diabetic hearts. Protein-protein interactions and modifications of the Carboxyl-terminus (CT) of CX43 are major determinants of GJ function, size, distribution and organization during normal development and disease processes. The extreme CT domain of CS43 interacts directly with the second PDZ domain of the MAGUK protein Zonula Occludens 1 (ZO-1). It has been reported that this interaction regulates phosphorylation of Cx43 and directs Cx43 GJ localization at the intercalated disk. Additionally, levels of this interaction are increased in the ischemic and dilated heart suggesting a role of Cx43 ZO-1 association in disease. Recent work from the Gourdie laboratory has ...
Dengue virus (DENV) and tick-borne encephalitis virus (TBEV) are flaviviruses, which can cause lethal hemorrhagic fever and encephalitis, respectively. Here, we demonstrate that the TBEV-NS5 and DENV-NS5 proteins use an internal binding mechanism to target human PDZ proteins. TBEV-NS5 has high affinity to regulating synaptic membrane exocytosis-2 (RIMS2) and Scribble, whereas DENV-NS5 binds primarily to the tight junction protein zonula occludens-1 (ZO-1). Targeting of TBEV-NS5 to the plasma membrane is stabilised by ZO-1; however, DENV-NS5 co-localises with ZO-1 in the nucleus. These interactions have potential important roles in the ability of flaviviruses to manipulate cell proliferation, junction permeability and the interferon pathways.. ...
Clone REA199 recognizes CD144 (VE-Cadherin), a 120 KDa type II cadherin. Cadherins are cell adhesion molecules and mediate Ca2+ dependent homophilic interactions. CD144 contains five extracellular cadherin (EC) domains and like other cadherins can interact directly via its C-terminus with cytoplasmic proteins such as β-catenin, plaktoglobin, and p120. Plaktoglobin und β-catenin bind to α-catenin, which in turn interacts with several actin-binding proteins, α-actinin, ajuba, zonula occludens-1 (ZO-1). Further indirect interactions of CD144 with partners such as SHP-2, VEGFR-2, Csk, and PAR-3, 6 allows CD144 to not only regulate the stability and strength of cell adhesion but also to serve functions such as sensing of shear forces, anti-proliferative, and anti-apoptotic effects. Additional information: Clone REA199 displays negligible binding to Fc receptors. - Schweiz
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PDZ (PSD95/Discs large/ZO-1) domains are ubiquitous protein interaction motifs found in scaffolding proteins involved in signal transduction. Despite the fact that many PDZ domains show a limited tendency to undergo structural change, the PDZ family has been associated with long-range communication and allostery. One of the PDZ domains studied most in terms of structure and biophysical properties is the second PDZ ("PDZ2") domain from protein tyrosine phosphatase 1E (PTP1E, also known as PTPL1). Previously, we showed through NMR relaxation studies that binding of the RA-GEF2 C-terminal peptide substrate results in long-range propagation of side-chain dynamic changes in human PDZ2 [Fuentes, E. J., et al. (2004) J. Mol. Biol. 335, 1105-1115]. Here, we present the first X-ray crystal structures of PDZ2 in the absence and presence of RA-GEF2 ligand, determined to resolutions of 1.65 and 1.3 Å, respectively. These structures deviate somewhat from previously determined NMR structures and indicate ...
TY - JOUR. T1 - Effect of cytokines on ICAM-1 and ZO-1 expression on human airway epithelial cells. AU - Shahana, Shahida. PY - 2005/9. Y1 - 2005/9. N2 - The presence of adhesion molecules on airway epithelial cells may be important in recruiting leukocytes to the epithelium. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. The cytokines IL-4, IL-8, IL-13 and IFN-gamma had only minor effects on cell proliferation in the columnar 16HBE14o-cells, and inhibited the effects of CHX on cell death. IFN-gamma increased ICAM-1 expression in both cell lines. Western blot analysis showed that CHX inhibited both ICAM-1 and ZO-1 expression in the basal cell line. A combination of IL-4 and IFN-gamma appeared to break ...
Congenital tufting enteropathy (CTE) is a severe autosomal recessive human diarrheal disorder with characteristic intestinal epithelial dysplasia. CTE can be caused by mutations in genes encoding EpCAM, a putative adhesion molecule, and HAI-2, a cell surface protease inhibitor. A similar phenotype occurs in mice whose intestinal epithelial cells (IECs) fail to express the tight junction-associated protein claudin-7. EpCAM stabilizes claudin-7 in IECs, and HAI-2 regulates the cell surface serine protease matriptase, a known modifier of intestinal epithelial physiology. Therefore, we hypothesized that HAI-2, matriptase, EpCAM, and claudin-7 were functionally linked. Herein we have demonstrated that active matriptase cleaves EpCAM after Arg80 and that loss of HAI-2 in IECs led to unrestrained matriptase activity and efficient cleavage of EpCAM. Cleavage of EpCAM decreased its ability to associate with claudin-7 and targeted it for internalization and lysosomal degradation in conjunction with ...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject-specific sections.
Clone REA199 recognizes CD144 (VE-Cadherin), a 120 KDa type II cadherin. Cadherins are cell adhesion molecules and mediate Ca2+ dependent homophilic interactions. CD144 contains five extracellular cadherin (EC) domains and like other cadherins can interact directly via its C-terminus with cytoplasmic proteins such as β-catenin, plaktoglobin, and p120. Plaktoglobin und β-catenin bind to α-catenin, which in turn interacts with several actin-binding proteins, α-actinin, ajuba, zonula occludens-1 (ZO-1). Further indirect interactions of CD144 with partners such as SHP-2, VEGFR-2, Csk, and PAR-3, 6 allows CD144 to not only regulate the stability and strength of cell adhesion but also to serve functions such as sensing of shear forces, anti-proliferative, and anti-apoptotic effects. Additional information: Clone REA199 displays negligible binding to Fc receptors. - Ireland
Yearly, millions of women and men in the United States return home from the hospital, with one or more drains inserted in the surgical site. The drains are placed during certain surgeries to carry fluids away from the surgical site, a process that helps with healing. A drain may need to stay in place for several days to several weeks. Unfortunately, traditional ways to secure these drains, such as use of safety pins, have significant drawbacks, such as tube punctures, which can frustrate patients.. After years of working with patients, and witnessing their frustration, discomfort, and anxieties with post-surgical drains, as well as the clinical complications that occurred related to not having a universal way to secure drainage bulbs, I decided it was time to find a safer, easier to use, alternative. Despite an extensive search, I could not find one, so I developed a belt-like surgical drainage bulb holder. Patients can wear the holder under clothing, while sleeping, and in the shower.. The ...
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This is Zoës Dad, Peter, I thought this blog of Zoës needed some attention as weve not posted very much recently. We aim to remedy that over the coming weeks so you can expect lots of content to be popping up.. Zoë has been extremely busy, with lots of gigs, writing new material and some time spent in the studio. Weve got a ton of photos and some video we want to share with you so be sure to subscribe so you can find out when all that gets published.. A lot of effort and time is spent on facebook so please make sure youre following Zoë there too. You can find her page by following this link: Zoës facebook page.. In the meantime, heres a sneak peek of her latest song, San Francisco. This is a live recording and Zoës also working on a studio version which may appear on a forthcoming release. Nothings decided just yet so youll just have to keep watching this space for more details.. ...
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sample_1: ZO2PDZ2 0.8 ± 0.1 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 1 mM. sample_2: ZO2PDZ2, [U-100% 15N], 0.8 ± 0.1 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 1 mM. sample_3: ZO2PDZ2, [U-100% 13C; U-100% 15N], 0.8 ± 0.1 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 1 mM. sample_4: ZO2PDZ2, [U-100% 13C; U-100% 15N], 0.8 ± 0.1 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 1 mM. sample_5: ZO2PDZ2, [U-100% 13C; U-100% 15N], 0.4 ± 0.05 mM; ZO2PDZ2 0.4 ± 0.05 mM; phosphate buffer 20 mM; NaCl 50 mM; EDTA 1 mM. sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 310 K ...
Zoë Kessler:Double Agent; an ADHDer goes undercover to interview Dr. Timothy Bilkey, a psychiatrist specializing in adult ADHD, at The Bilkey Clinic in Barrie
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Meerdere linken in de vroegere artikelen zullen vermoedelijk niet werken, maar u kan de titel dan kopiëren en plakken in het "Zoek" venster om zo bij het juiste artikel te komen. Ik maak ere wrek van om de artikelen vanaf 2005 terug te editen en daarin de linken te vernieuwen zodat zij terug zullen werken. Maar dit vraagt enige tijd. Daarom verzoek ik hiervoor begrip teh hebben indien u een link tegen komt die niet meer werkt ...
Wšědny wowsnik je trajne zelo, kotrež docpěwa wysokosć wot 50 hač 150 cm. Łopjena docpěwaja šěrokosć wot 4 hač 9 mm a su płone, nahe a na hornim boku tróšku hrube. Kćěje wot junija hač julija. Pakić docpěwa dołhosć wot 25 (10-20) cm. Hač do 1 cm dołhe, błyšćace, často wioletnje blakate kłóski njesu 1 dołhi kocht, kotryž je 5-10 mm dlěši hač kłóska. Delnja přikrywna pluwiznapluwizna je jednonerwowa, mjeztym zo hornja je třonerwowa. ...
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