TY - JOUR. T1 - Outlier analysis defines zinc finger gene family DNA methylation in tumors and saliva of head and neck cancer patients. AU - Gaykalova, Daria A.. AU - Vatapalli, Rajita. AU - Wei, Yingying. AU - Tsai, Hua Ling. AU - Wang, Hao. AU - Zhang, Chi. AU - Hennessey, Patrick T.. AU - Guo, Theresa. AU - Tan, Marietta. AU - Li, Ryan. AU - Ahn, Julie. AU - Khan, Zubair. AU - Westra, William H.. AU - Bishop, Justin A.. AU - Zaboli, David. AU - Koch, Wayne M.. AU - Khan, Tanbir. AU - Ochs, Michael F.. AU - Califano, Joseph A.. N1 - Funding Information: The analysis in this article is based on a web database applications provided by Research Information Technology Systems (RITS)-https://www.rits.onc.jhmi.edu/. This work was supported by the National Institute of Dental and Craniofacial Research and National Institute of Health Challenge Grant (RC1DE020324); National Institute of Dental and Craniofacial Research and National Cancer Institute grant (P50 DE 019032 Head and Neck Cancer SPORE) to ...

Spodoptera frugiperda SF-21 cells infected with Autographa californica nuclear polyhedrosis virus mutants which lack a functional p35 gene undergo apoptosis, a type of programmed cell death. To identify p35-homologous genes in other baculoviruses, A. californica nuclear polyhedrosis virus DNA containing a deletion in p35 was cotransfected into SF-21 cells along with genomic DNAs from other baculoviruses. One of the viral DNAs which were able to rescue wild-type infection was from Cydia pomonella granulosis virus (CpGV). The CpGV gene responsible for the effect was mapped to a 1.6-kb SalI-SstI subclone of the SalI B fragment of CpGV. The sequence of the SalI-SstI subclone revealed an open reading frame capable of encoding a polypeptide of 31 kDa which was sufficient to rescue wild-type infection; this gene was thus called iap (inhibitor of apoptosis). The predicted sequence of the IAP polypeptide exhibited no significant homology to P35 but contained a zinc finger-like motif which is also found ...

TY - JOUR. T1 - A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. AU - Yamaji, Naoki. AU - Huang, Chao Feng. AU - Nagao, Sakiko. AU - Yano, Masahiro. AU - Sato, Yutaka. AU - Nagamura, Yoshiaki. AU - Ma, Jian Feng. PY - 2009/10. Y1 - 2009/10. N2 - Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray ...

PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.

In molecular genetics, the Krüppel-like family of transcription factors (KLFs) are a set of zinc finger DNA-binding proteins that regulate gene expression. The following human genes encode Kruppel-like factors: KLF1, KLF2, KLF3, KLF4, KLF5, KLF6, KLF7, KLF8, KLF9, KLF10, KLF11, KLF12, KLF13, KLF14, KLF15, KLF16, KLF17 KLFs are a family of transcription factors that contain three carboxyl-terminal (C-terminal) C2H2-type zinc finger structural motifs that bind to the GC-rich regions in DNA and regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. The C-terminal end binds to the promoter and enhancer regions of a gene. Each KLF also has a unique amino-terminal (N-terminal) end that acts as the functional domain that allows it to bind specifically to a certain partner. KLFs share the similar function of transcription regulation via the recruitment of regulatory proteins. These transcription ...

Nucleases are the enzyme, used to cleave DNA into smaller units. Zinc-finger (ZFN) nucleases are artificial restriction enzyme used to cleave DNA into smaller fragments. It is the class of engineered DNA-binding proteins that creates double standard break at specified locations. It consist of two functional domain, a DNA-binding domain, and a DNA-cleaving domain. DNA binding domain recognizes the unique hexamer sequence of DNA and DNA-cleaving domain consisting nuclease domain of Fok I. The fusion between the DNA-binding domain, and a DNA-cleaving domain creates artificial restriction enzyme known as molecular scissor that cleaves the desired DNA sequence. ZFN is based on the DNA repair machinery and is becoming a prominent tool in the field of genome editing.. Zinc finger nucleases are useful for various biotechnological and life science applications. It is used to manipulate plants and animals for research purpose and is used in the clinical trial of CD4+ human T-cells for the treatment of ...

The first three zinc fingers (ZF1-3) of transcription factor IIIA (TFIIIA) from Xenopus have been shown to contribute the majority of the binding energy to the intact TFIIIA-DNA interaction [Liao et al. (1992) J. Mel. Biol, 223, 857-871]. We have expressed a 92-amino acid polypeptide containing the three N-terminal zinc fingers of TFIIIA. This three-fingered polypeptide has

CCCH zinc finger alignments in Postia placenta . Alignments can be refined by adding alignments from other genomes, adding your own sequences and/or aligning to other models from the same superfamily. The display of alignments can also be customised.

Krüppel-like factor (KLF) family members share a three C2H2 zinc finger DNA binding domain, and are involved in cell proliferation and differentiation control in normal as in pathological situations. KLFs can be deregulated in multiple cancers either by loss of heterozygosity (LOH), somatic mutation or transcriptional silencing by promoter hypermethylation. KLF family member proteins play a critical role in the growth and metastasis of numerous tumor types, at least in part by regulating the expression of cell cycle genes. Globally, KLF4 and KLF6 are considered as tumor suppressor gene, whereas KLF5 promotes cell proliferation. Family members have different transcriptional properties and can modulate each others activity by a variety of mechanisms. Since cells can express multiple KLFs, KLF transcription factors build likely a transcriptional network to control cell proliferation. Effects of changes in KLF factors are context-dependent and can appear contradictory, considering differences in ...

From NCBI Gene:. In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5 exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5 exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]. From UniProt: ...

GATA-1 and FOG-1 zinc fingers. Computer model showing the complex of the zinc finger transcription factors GATA-1 (orange) and FOG-1 (friend of GATA, blue). The two zinc ions shown in grey become visible after removing a few atoms hiding them. - Stock Image C035/5609

Colorado Maine Coon Cattery also breeds polydactyl kittens at $2,000 each with a $550 shipping cost if you are unable to pick them up personally. Polydactyl Manx Kittens for sale in Boise, Idaho $150 Share it or review it. Kindly content or require details. If you are interested in purchasing a manx or polydactyl cat or kitten, please E-Mail Us and we will send photos and put you on our waiting list. The information on Amber Technologys There are several kittens to choose from. Supplement Health & Education Act (DSHEA) of 1994 requires us to state: These statements have not been evaluated by the Food and Drug Administration. It is highly recommended you research and Look at pictures of Manx kittens who need a home. Please enable Cookies and reload the page. Being used to dogs would be a benefit, but not a must (if shes young). Let us search for you! These cats are strong, healthy, bright eyed, playful, have strong immune systems, and do NOT get sick - naturally. Herbal Nutraceuticals for Pets ...

Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene ...

Genetic analysis and homology between the phenotypic alterations of the human Greig Cephalopolysyndactyly Syndrome (GCPS) and the mouse mutant extra-toes (Xt) have suggested a dominant mutation in the same gene of both species. Recently, the GLI3 gene, a member of the Kruppel-related zinc finger genes, has been proposed as a candidate gene for GCPS. We examined the expression of the mouse Gli3 gene in both Xt mutant animals and during normal mouse development. Northern and RNAase protection analysis of embryos revealed that Gli3 expression was reduced about 50% in heterozygous Xt/+ mice and completely absent in homozygous Xt/Xt mice. In addition, in situ analysis of wild-type mice documented Gli3 expression in the developing limb and brain, structures affected in Xt mutant mice. This pattern suggests an important function of the Gli3 gene during morphogenesis.. ...

National Cancer Institute. Evi3 is a common site of retroviral integration in B-cell lymphomas of AKXD mice. BLAST searches of Evi3 genomic sequences against the mouse and human databases showed that most viral integrations at Evi3 are located immediately upstream of the first translated exon (exon 2) of a gene encoding a novel zinc finger protein with 30 kr ppel-like zinc finger repeats. Viral integrations at Evi3 upregulate the expression of this gene via promoter sequences present in the viral long terminal repeat. EVI3 protein is highly related to the early B-cell associated zinc finger protein EBFAZ, and all 30 zinc fingers found in EVI3 are conserved in EBFAZ. Ebfaz and Evi3 are coexpressed in many cell types, and EVI3, like EBFAZ, is located in the nucleus. EBFAZ binds to, and negatively regulates, early B cell factor (EBF), a basic helix-loop-helix transcription factor required for B-cell-lineage commitment. EBFAZ also binds to SMAD1 and SMAD4 in response to BMP2 signaling, which in turn ...

Education. -1998 PhD (4 years program) at University of Rome La Sapienza in Human Biology: Cellular and Molecular Biology Bases. -1991 Degree in Biology, University of Rome La Sapienza Department of Human Biopathology. University of Rome La Sapienza, Rome, Italy. Experimental Thesis, summa cum laude.. Current position and research experience. -From 2009 to present CNR Researcher (III level) at Istituto Biologia e Patologia Molecolari (IBPM), CNR, Rome, Italy.. -From 1999 to 2008 post-doctoral fellow at CNR, Istituto Biologia e Patologia Molecolari (IBPM), Rome, Italy.. -From 1994 to1998 PhD (4 years program) at University of Rome La Sapienza in Human Biology: Cellular and Molecular Biology Bases. Thesis on artificial zinc finger transcription factors programmed to upregulate utrophin gene in Duchenne Muscular Dystrophy (DMD). Supervisor: Dr. Claudio Passananti.. -From 1995 to 1993 research experience as PhD/fellow in the field of Regulation of gene expression and development in the ...

PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.

In order to study the mechanism of neural patterning in Xenopus, we used subtractive cloning to isolate genes activated early during this process. One gene isolated was opl, (odd-paired-like) that resembles the Drosophila pair-rule gene odd-paired and encodes a zinc finger protein that is a member of the Zic gene family. At the onset of gastrulation, opl is expressed throughout the presumptive neural plate, indicating that neural determination has begun at this stage while, by neurula, opl expression is restricted to the dorsal neural tube and neural crest. opl encodes a transcriptional activator, with a carboxy terminal regulatory domain, which when removed increases opl activity. opl both sensitizes animal cap ectoderm to the neural inducer noggin and alters the spectrum of genes induced by noggin, allowing activation of the midbrain marker engrailed. Consistent with the later dorsal neural expression of opl, the activated form of opl is able to induce neural crest and dorsal neural tube ...

Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA. The epithelium lining the intestine undergoes rapid and continuous renewal. Growth factors play a role in intestinal epithelial growth regulation in vitro and in vivo. In this study, transforming growth factor alpha (TGF alpha) is shown to act as a mitogen and induce the expression of two zinc finger-containing immediate early genes [Zif268 (zinc finger protein 268) and Nup475 (nuclear protein 475)] in rat intestinal epithelial (RIE-1) cells in culture. These two gene products were initially isolated from serum-treated fibroblasts and represent growth-stimulated transcription factors. In TGF alpha-treated RIE-1 cells, nuclear run-on experiments demonstrate that TGF alpha induction of these two genes is regulated predominantly at the level of gene transcription. Utilizing in situ hybridization techniques, we show that systemic administration of TGF alpha induces expression of these two genes in the rat ...

Exploring the Recognition of Quadruplex DNA by an Engineered Cys2-His2 Zinc Finger Protein. Biochemistry 45 (5) , pp. 1393-1399. 10.1021/bi050229x ...

1CS3: Structure-function studies of the BTB/POZ transcriptional repression domain from the promyelocytic leukemia zinc finger oncoprotein.

The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. UBZ domains coordinate a zinc ion with cysteine or histidine residues; depending on their amino acid sequence, UBZ domains are classified into several families [1,2]. Type 1 UBZs are CCHH-type zinc fingers found in tandem UBZ domains of TAX1-binding protein 1 (TAX1BP1) [3,4,5], type 2 UBZs are CCHC-type zinc fingers found in FAAP20 which is a subunit of the Fanconi anemia (FA) core complex [6,7], type 3 UBZs are CCHH-type zinc fingers found only in the Y-family translesion polymerase eta [8,9,10], and type 4 UBZs are CCHC-type zinc fingers found in Y-family translesion polymerase kappa, Werner helicase-interacting protein 1 (WRNIP1), and Rad18 [11,12,13]. The UBZ domain consists of two short antiparallel β-strands followed by one α-helix. The α-helix packs against the β-strands with a zinc ion sandwiched between the ...

Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases - Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided ...

Zinc is an essential trace element that plays a crucial role in catalytic, structural and regulatory functions of many proteins including enzymes and transcription factors; thus maintenance of zinc balance is critical for normal cellular function. Cellular mechanisms that maintain zinc balance include the regulation of genes coding for proteins that play vital roles in zinc homeostasis. These proteins include zinc transporters belonging to the ZIP (SLC39A) and ZnT (SLC30A) families as well as the zinc-binding metallothionein proteins. In contrast to bacterial and yeast systems, a transcription factor responsible for mediating transcriptional repression of a suite of genes in response to elevated zinc levels in mammals has hitherto not been identified. Using Caco-2 cells as a model of human intestinal epithelial cells and detection of protein binding by electrophoretic mobility shift analysis, we show that zinc finger protein ZNF658 binds specifically to the zinc transcriptional regulatory ...

Inhibitor of apoptosis proteins (IAPs) constitute a conserved family of molecules, which regulate both apoptosis and receptor signalling. They are often deregulated in cancer cells and represent potential targets for therapy. In my work, I investigated the effect of IAP inhibition in vivo to identify novel down-stream genes expressed in an IAP-dependent manner that could contribute to cancer aggressiveness. To this end, immunocompromised mice engrafted subcutaneously with the triple negative breast cancer (TNBC) cell line MDA-MB231 were treated with SM83, a Smac mimetic developed in our laboratory that acts as a pan-IAP inhibitor, and tumour nodules were profiled for gene expression. The analysis revealed that the inhibition of IAPs significantly reduces the expression of SNAI2, a zinc finger transcriptional repressor often associated with cancer aggressiveness, resistance to therapy and metastatic potential, especially in breast cancer. By testing several TNBC cell lines, I found that SNAI2 ...

Krüppel-like factor 5 (intestinal) or Krüppel-like factor 5 (KLF5) is a zinc finger-containing transcription factor and involved in important biological processes including cell proliferation and differentiation. However, clinical significance of KLF5 protein has remained largely unknown in breast cancer. Therefore, in this study, we immunolocalized KLF5 in 113 human breast carcinoma cases. KLF5 immunoreactivity was frequently detected in the nuclei of breast carcinoma cells, and median value of the ratio of KLF5-positive carcinoma cells was 30% and was positively associated with the status of androgen receptor. KLF5 immunoreactivity was also significantly associated with increased risk of recurrence and worse clinical outcome in breast cancer patients by univariate analyses, and subsequent multivariate analyses demonstrated that KLF5 immunoreactivity was an independent prognostic factor for both disease-free and breast cancer-specific survival of the patients. We then examined possible regulation of

TY - JOUR. T1 - Finger inter-dependence. T2 - Linking the kinetic and kinematic variables. AU - Kim, Sun Wook. AU - Shim, Jae Kun. AU - Zatsiorsky, Vladimir M.. AU - Latash, Mark L.. N1 - Funding Information: This study was supported in part by NIH Grants AG-018751, AR-048563, and NS-35032.. PY - 2008/6. Y1 - 2008/6. N2 - We studied the dependence between voluntary motion of a finger and pressing forces produced by the tips of other fingers of the hand. Participants moved one of the fingers (task finger) of the right hand trying to follow a cyclic, ramp-like flexion-extension template at different frequencies. The other fingers (slave fingers) were restricted from moving; their flexion forces were recorded and analyzed. Index finger motion caused the smallest force production by the slave fingers. Larger forces were produced by the neighbors of the task finger; these forces showed strong modulation over the range of motion of the task finger. The enslaved forces were higher during the flexion ...

Each competitor and mentor would love to have something that would avoid finger wounds. A torn tendon can prompt to lessen playing time for a competitor. There is additionally the danger of lasting handicap. These conceivable outcomes cause numerous competitors to wear a finger prop practically speaking and even in amusements. Concentrates on have demonstrated that the prophylactic finger support can forestall harm yet there are clashing reports also. The finger is uncovered and exceptionally helpless amid athletic action and in view of its huge size it is frequently harmed. In sports, finger supports have two purposes. They are intended to ensure the competitor with past finger wounds. The finger prop underpins the finger to diminish torment. It likewise keeps the finger from being harmed again as it mends. The prop additionally ensures the finger amid substantial physical games and keeps the finger from being harmed. Browse around this website ...

Dergai, O., Cousin, P., Gouge, J., Satia, K., Praz, V., Kuhlman, T., Lhote, P., Vannini, A., Hernandez, N. (May 2018) Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. Genes Dev, 32 (9-10). pp. 711-722. ISSN 0890-9369 Denissov, S., Van Driel, M., Voit, R., Hekkelman, M., Hulsen, T., Hernandez, N., Grummt, I., Wehrens, R., Stunnenberg, H. (February 2007) Identification of novel functional TBP-binding sites and general factor repertoires. Embo Journal, 26 (4). pp. 944-954. ISSN 0261-4189 Emran, F., Florens, L., Ma, B., Swanson, S. K., Washburn, M. P., Hernandez, N. (August 2006) A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription complexes. Gene, 377. pp. 96-108. ISSN 0378-1119 (Print) Kim, Y. S., Kim, J. M., Jung, D. L., Kang, J. E., Lee, S., Kim, J. S., Seol, W., Shin, H. C., Kwon, H. S., Van Lint, C., Hernandez, N., Hur, M. W. (June 2005) Artificial zinc finger fusions targeting Sp1-binding sites and the trans-activator-responsive element ...

Zinc finger protein 64 homolog, isoforms 1 and 2 is a protein that in humans is encoded by the ZFP64 gene. Homolog Protein isoform Zinc finger GRCh38: Ensembl release 89: ENSG00000020256 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000027551 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Mack HG, Beck F, Bowtell DD (Mar 1997). A search for a mammalian homologue of the Drosophila photoreceptor development gene glass yields Zfp64, a zinc finger encoding gene which maps to the distal end of mouse chromosome 2. Gene. 185 (1): 11-7. doi:10.1016/S0378-1119(96)00607-5. PMID 9034307. Entrez Gene: ZFP64 zinc finger protein 64 homolog (mouse). Maruyama K, Sugano S (1994). Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138 (1-2): 171-4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). Construction and characterization of a full ...

Author Summary The ability of sexually reproducing organisms to produce viable offspring depends on their ability to faithfully execute meiosis. Meiosis is a specialized set of two cell divisions that ensures that each sperm and egg receives only one copy of each pair of chromosomes. Thus, in human females, although virtually all somatic cells carry 23 pairs of homologous chromosomes (for a total of 46 chromosomes), the egg needs to possess only one copy of each chromosome (for a total of 23). This reduction in chromosome number requires three basic steps: the pairing of homologous chromosomes, the linking of those pairs by recombination, and the separation of those pairs into two daughter cells at the first meiotic division. Unfortunately, little is known about the mechanism(s) by which the sites of recombination are chosen. Here we describe a Drosophila protein called Trem that both binds to meiotic chromosomes and defines the first known step of recombination initiation in Drosophila. Our studies of

hypothetical protein, PAC1, Anapl_07643, AS27_06939, AS28_03976, bcd1, B-cell-derived protein 1, C86813, CB1_000294041, cba1, copeb, core promoter element binding protein, core promoter element-binding protein, cpbp, FM2, FM6, GBF, GC-rich binding factor, GC-rich sites-binding factor GBF, Ierepo1, Ierepo3, immediate early response, erythropoietin 1, klf6-a, klf6-b, Krueppel-like factor 6, Krueppel-like factor 6-like, Krueppel-like factor 7, Kruppel-like factor 6, Kruppel-like zinc finger protein Zf9, M91_01956, M959_03695, MDA_GLEAN10016415, N300_13616, N301_13483, N302_03995, N303_03824, N305_14493, N306_00708, N307_00582, N308_06795, N309_01017, N312_07173, N320_02403, N321_14125, N322_09693, N324_04458, N325_02508, N326_01608, N327_05534, N328_08868, N329_08139, N330_10147, N331_09768, N332_08191, N334_00881, N335_03384, N336_10967, N339_05665, N340_06919, N341_10990, PAL_GLEAN10001872, PANDA_010138, proto-oncogene BCD1, protooncogene B-cell derived 1, R75280, ST12, suppression of ...

Its been a while since weve seen something from Amon Tobins alter ego - Two Fingers, but thats gonna get fixed really soon. A brand new EP. Check out Six Rhythms by Two Fingers, Noisia on Beatport. Two Fingers, the alias of our longtime friend Amon Tobin emerges on Division. Six new tracks including a Noisia feature, pressed on 1 x grams 12 vinyl. Mixmag - Premiere: Two Fingers - Cashew Rhythm (Original) Amon Tobin - Two Fingers - Saint Rhythm [Nest HQ Premiere] Amon Tobin - Two Fingers - Tasm Fet. Two Fingers, the alias of our longtime friend Amon Tobin emerges on Division. Six new tracks including a Noisia feature. Artwork by. View credits, reviews, tracks and shop for the Vinyl release of Six Rhythms on Discogs. Discover releases, reviews, credits, songs, and more about Two Fingers - Six Rhythms at Discogs. Complete your Two Fingers collection. Six Rhythms. By Two Fingers. • 6 songs. Play on Spotify. 1. Cashew Listen to Six Rhythms in full in the Spotify app. Play on Spotify. Playing. ...

ZC3H10 - ZC3H10 (GFP-tagged) - Human zinc finger CCCH-type containing 10 (ZC3H10) available for purchase from OriGene - Your Gene Company.

Notably, under conditions in which no killing of cells occurred, exposure of yeast over hundreds of generations to increasing concentrations of AmB has yielded resistant strains with permanent changes in the expression of genes such as yor1 and pdr16 (41), which are members of the ATP-binding cassette (ABC) family of transporters (9). The activation of yor1 and pdr16 is controlled by the zinc finger transcription factors Pdr1 and Pdr3, which activate proteins involved in multidrug resistance and in the translocation of plasma membrane phospholipids (9). Among the stably overexpressed genes that also confer resistance to AmB (41) are ict1, which encodes a lysophosphatidic acid acyltransferase that is responsible for enhanced phospholipid synthesis and increased resistance to antifungal drugs, and ygr035C and ypl088, which are activated by Yrm1q and Yrr1, the yeast zinc finger transcription factors which are also controlled by the pleiotropic drug resistance (PDR) gene network (9).. Another yeast ...

Buy our Recombinant Human MBD2 Interacting Zinc Finger MIZF protein. Ab161859 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA…

The binding of Au(iii) complexes to the zinc finger domain of the anticancer drug target PARP-1 was studied using a hyphenated mass spectrometry approach combined with quantum mechanics/molecular mechanics (QM/MM) studies. Competition experiments were carried out, whereby each Au complex was exposed to two t

LIM (Lin-1, Isl1, Mec3) domain proteins all contain least one double zinc finger motif (Fig. 1). LIM family proteins contain between one and five LIM domains plus other that have specific functions such as actin-binding, kinases, and nuclear translocation motifs. We are the first to examine 33 LIM proteins (including three that bind to but do not themselves contain LIM domains) that are implicated in either the development of the heart, heart disorders and failure or both.. We then assembled a cellular snapshot of the LIM proteins known to be expressed in the heart that helps explain how mutations in these proteins may play a role in the development of heart failure. Furthermore, we name the complexity of LIM domain protein interactions as a LIM interactome.. ...

Numbness in right index finger - Hi, I have a random, tingling, and numbness to my right index finger at random times of the day. I havent hit my arm or jammed my finger or any sorts? More information. Many possibilities, more information is needed. If one taps on your median nerve in the wrist does your finger tingle. If one puts pressure on the proper digital nerve in the palm or finger can you reproduce the symptoms. If the problem was in a nerve root (C6) you would also get shooting pains to from the neck down to the finger. Time will tell. It will either or worse.

Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five...

The zinc-finger transcription factor Insulinoma-associated 1 (Insm1, previously IA-1) is expressed in the developing anxious and neuroendocrine systems, and is required for cell type specific differentiation. maintained in accordance with established protocols for zebrafish husbandry (Westerfield, 1995). Larvae and Embryos had been housed at 28C, on the 14 h light:10 h dark routine. Fish had been anaesthetized with Ethyl 3-aminobenzoate methanesulfonate sodium (MS-222, Tricaine, Sigma-Aldrich, St. Louis, MO). Embryos had been staged as previously referred to (Kimmel et al., 1995). Crazy type strains included the Ekwill stress (Ekwill Fish Plantation, Gibsonton, FL), the Stomach strain extracted from the Zebrafish International Analysis Middle (ZIRC, Eugene, OR) and hybrids made by crossing the Ekwill and Stomach strains. The Tg MK-0812 (XRho: distance43-mCFP) q13 transgenic range, called XOPS-mCFP hereafter, continues to be previously referred to (Morris et al., 2011; Morris et al., 2008a). This ...

Arsenic, an ancient drug used in traditional Chinese medicine, has attracted wide interest because it has therapeutic activity in patients with acute promyelocytic leukemia (APL). The drug acts by promoting degradation of an oncogenic protein, PML-RARα, a fusion protein containing sequences from the PML zinc finger protein and retinoic acid receptor α, which is found specifically in APL cells and helps drive their growth. Zhang et al. (see the Perspective by Kogan) now explain how arsenic initiates the molecular events leading to PML-RARα degradation. Arsenic was found to bind directly to cysteine residues within zinc finger domains of PML. Arsenic binding then induced oligomerization of PML, which in turn enhanced its association with an enzyme that helps catalyze SUMOylation, a posttranslational modification that can target proteins for degradation.. X.-W. Zhang, X.-J. Yan, Z.-R. Zhou, F.-F. Yang, Z.-Y. Wu, H.-B. Sun, W.-X. Liang, A.-X. Song, V. Lallemand-Breitenbach, M. Jeanne, Q.-Y. ...

Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...

Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...

Substance P is a member of the tachykinin family of neuropeptides that plays an important role in pain transmission, neurogenic inflammatory diseases and the adaptive response to stress. Substance P exerts its biological activities via binding to a G-protein coupled receptor of the neurokinin (NK) receptor family. Here, we show by Western blot experiments that substance P induced a transient synthesis of the zinc finger transcriptional regulator Egr-1 in human glioma cells. Substance P-induced stimulation of Egr-1 biosynthesis was completely inhibited by the mitogen-activated protein kinase kinase inhibitor PD98059 and by AG1487, an epidermal growth factor (EGF) receptor-specific tyrosine kinase inhibitor. These results indicate that transactivation of the EGF receptor as well as stimulation of the mitogen activated/extracellular signal-regulated protein kinase (ERK) are essential for substance P/NK-1 receptor-induced activation of Egr-1 biosynthesis. Moreover, we show that the signaling cascade

Two-dimensional NMR studies of the zinc finger motif: solution structures and dynamics of mutant ZFY domains containing aromatic substitutions in the

DNA-binding domain of thyroid hormone receptors (TRs) is composed of two C4-type zinc fingers. DNA-binding domain of thyroid hormone receptors (TRs) is composed of two C4-type zinc fingers. Each zinc finger contains a group of four Cys residues which co-ordinates a single zinc atom. TR interacts with the thyroid response element, which is a DNA site with direct repeats of the consensus sequence 5-AGGTCA-3 separated by one to five base pairs, upstream of target genes and modulates the rate of transcriptional initiation. Thyroid hormone receptor (TR) mediates the actions of thyroid hormones, which play critical roles in growth, development, and homeostasis in mammals. They regulate overall metabolic rate, cholesterol and triglyceride levels, and heart rate, and affect mood. TRs are expressed from two separate genes (alpha and beta) in human and each gene generates two isoforms of the receptor through differential promoter usage or splicing. TRalpha functions in the heart to regulate heart rate ...

Oops. Have omitted to comment on apparent finger width, depending on whether the fingers are held together or spread apart. Notice anything? The fingers look more spindly and unreal in the imprints where they are touching. Reason? The linen tends to bridge the fingers, being unable to penetrate the narrow groove that separates them . When the fingers are held apart, the linen can loop down between the fingers, making contact with more of their sides. Result: some lateral imaging, aka distortion, that makes the fingers look fatter than in the bunched-together configuration. Having said that, the fat fingers are probably closer to their real width, as measured in a photograph, than the spindly ones. The spindly fingers of the man on the Shroud are prima facie evidence for imaging by contact, and ONLY by contact. Its time that imaging via models that depend on collimated radiation of unspecified wavelength, severely attenuated by air to explain 3D properties and apparent 3.7cm cut-off, are ...

The problem with doing a finger joint with all square fingers is that the maximum strength of the joint is the strength of the fingers. But the fingers can only be half as strong as the pieces of wood they join, seeing that half is cut away for the other fingers. When buying finger joined material, the fingers are always trapezoidal, or triangular. Provided that the glue is strong enough, and the angle narrow enough, this can produce a joint that is nearly as strong as the wood it joins. Ideally, I would have a sawblade that makes a trapezoidal cut, but such a thing doesnt exist, so I experimented a little with trying other variations on the end to end finger joint. ...

Ikaros family zinc finger 1 (IKZF1) is a haematopoietic transcription factor required for mammalian B-cell development. IKZF1 deficiency also reduces plasmacytoid dendritic cell (pDC) numbers in mice, but its effects on human DC development are unknown. Here we show that heterozygous mutation of IKZF1 in human decreases pDC numbers and expands conventional DC1 (cDC1). Lenalidomide, a drug that induces proteosomal degradation of IKZF1, also decreases pDC numbers in vivo, and reduces the ratio of pDC/cDC1 differentiated from progenitor cells in vitro in a dose-dependent manner. In addition, non-classical monocytes are reduced by IKZF1 deficiency in vivo. DC and monocytes from patients with IKZF1 deficiency or lenalidomide-treated cultures secrete less IFN-α, TNF and IL-12. These results indicate that human DC development and function are regulated by IKZF1, providing further insights into the consequences of IKZF1 mutation on immune function and the mechanism of immunomodulation by lenalidomide. ...

Angiogenesis is meticulously controlled by a fine balance between positive and negative regulatory activities. Vascular endothelial growth factor (VEGF) is a predominant angiogenic factor and its dosage is precisely regulated during normal vascular formation. In cancer, VEGF is commonly overproduced, resulting in abnormal neovascularization. VEGF is induced in response to various stimuli including hypoxia; however, very little is known about the mechanisms that confine its induction to ensure proper angiogenesis. Chromatin insulation is a key transcription mechanism that prevents promiscuous gene activation by interfering with the action of enhancers. Here we show that the chromatin insulator-binding factor CTCF binds to the proximal promoter of VEGF. Consistent with the enhancer-blocking mode of chromatin insulators, CTCF has little effect on basal expression of VEGF but specifically affects its activation by enhancers. CTCF knockdown cells are sensitized for induction of VEGF and exhibit elevated

Prader-Willi syndrome (PWS) is characterized by neonatal hypotonia, developmental delay and hyperphagia/obesity and is caused by the absence of paternal contribution to chromosome 15q11-q13. Using induced pluripotent stem cell (iPSC) models of PWS, we previously discovered an epigenetic complex that is comprised of the zinc-finger protein ZNF274 and the SET domain bifurcated 1 (SETDB1) histone H3 lysine 9 (H3K9) methyltransferase and that silences the maternal alleles at the PWS locus. Here, we have knocked out ZNF274 and rescued the expression of silent maternal alleles in neurons derived from PWS iPSC lines, without affecting DNA methylation at the PWS-Imprinting Center (PWS-IC). This suggests that the ZNF274 complex is a separate imprinting mark that represses maternal PWS gene expression in neurons and is a potential target for future therapeutic applications to rescue the PWS phenotype.. ...

GATA transcription factors mediate cell differentiation in diverse tissues, and their dysfunction is associated with certain congenital human disorders. The six classical vertebrate GATA proteins, GATA-1 to GATA-6, are highly homologous, bear two tandem zinc fingers of the C(4) (GATA) type, and activate transcription. TRPS1, the only other vertebrate protein with the GATA motif, is a large, multitype zinc finger protein that harbors a single DNA-binding GATA domain and represses transcription. Monoallelic TRPS1 mutations cause two dominantly inherited human developmental disorders of the hair, face, and digits, tricho-rhino-phalangeal syndrome (TRPS) types I (MIM 190350) and III (MIM 190351); missense GATA domain mutations account for the more severe type III form. Here we report that heterozygous mice with deletions of the TRPS1 GATA domain (TRPS1(+/Deltagt)) display facial anomalies that overlap with findings for TRPS, whereas TRPS1(Deltagt/Deltagt) mice additionally reveal a complete

The novel zinc finger protein 121 (ZNF121) has been demonstrated to physically and functionally associate with the MYC oncoprotein to regulate cell proliferation and likely breast cancer development. To further understand how ZNF121 functions in cell proliferation and carcinogenesis, we identified and characterized the interaction of ZNF121 with zinc finger and BRCA1-interacting protein with a KRAB domain 1 (ZBRK1), a breast and ovarian cancer susceptibility protein 1 (BRCA1)-interacting protein, using the yeast two-hybrid assay and other approaches. We also found that ZNF121 bound to BRCA1. Functionally, ZFN121 suppressed the expression of ANG1 and HMGA2, two common downstream targets of ZBRK1 and BRCA1. Interestingly, ZNF121 also regulated the expression of BRCA1 and ZBRK1. These findings suggest that ZNF121 is likely a member of the BRCA1/CtIP/ZBRK1 repressor complex that plays a role in breast cancer ...

The novel zinc finger protein 121 (ZNF121) has been demonstrated to physically and functionally associate with the MYC oncoprotein to regulate cell proliferation and likely breast cancer development. To further understand how ZNF121 functions in cell proliferation and carcinogenesis, we identified and characterized the interaction of ZNF121 with zinc finger and BRCA1-interacting protein with a KRAB domain 1 (ZBRK1), a breast and ovarian cancer susceptibility protein 1 (BRCA1)-interacting protein, using the yeast two-hybrid assay and other approaches. We also found that ZNF121 bound to BRCA1. Functionally, ZFN121 suppressed the expression of ANG1 and HMGA2, two common downstream targets of ZBRK1 and BRCA1. Interestingly, ZNF121 also regulated the expression of BRCA1 and ZBRK1. These findings suggest that ZNF121 is likely a member of the BRCA1/CtIP/ZBRK1 repressor complex that plays a role in breast cancer ...