NutritionFacts.org, by Michael Greger: In 1979, an epidemic of breast enlargement was noted in Italian children. Poultry or veal was suspected, given that estrogens may be fed to farm animals to accelerate their weight gain. After this episode, the European Union banned the application/use of anabolic growth promoters in agriculture, as well as the importation of American meat from animals injected with drugs like Zeranol, sold as Ralgro Magnum.. Zeranol, one of the most potent known endocrine disrupters, is 100,000 times more estrogenic than the plastics chemical, BPA, for example, and is the subject of my video Zeranol Use in Meat and Breast Cancer. Zeranol constitutes a special case among potential endocrine disrupters, because Zeranol, in contrast to all other oestrogenic endocrine disrupting chemicals, is present in human food because it is deliberately used in the production of consumer products. Furthermore, Zeranol is designed to be a potent, fairly persistent [estrogen] whereas ...

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This study was conducted to assess the impact of implanting intact beef males with protein anabolic agents at varying intervals throughout life. Ninety-six intact males were assigned to three implant treatments: 1) not implanted, 2) implanted at 9 wk of age, weaning and at 56-d intervals thereafter with a 36-mg zeranol implant or 3) estradiol implant at 9 wk of age and 68 d post-weaning. During the 118-d, post-weaning growing period, eight animals per treatment (one replication) were castrated. After a 114-d finishing period, cattle were slaughtered (average age of 13 to 14 mo). Feedlot performance, carcass and palatability data were obtained. Average daily gains and feed efficiency did not differ (P|.05) between zeranol and estradiol-implanted intact males. Regardless of implant treatment, steers had lighter carcass weights (P|.05) and higher (P|.01) quality grades than intact males. Implanting either intact males or steers with zeranol or estradiol resulted in higher (P|.05) numerical yield grades.

Mycotoxins are low-molecular-weight natural products produced, as secondary metabolites, by filamentous fungi. These molecules represent a wide chemical group with different toxicity effects in human and other animals. These toxins can accidentally occur in food and feed, due to a direct or indirect contamination. The aim of this work was to develop a method for the first time to quantitatively determine zearalenone and its metabolites (α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol, zearalanone) in bovine and human hair using LC-MS/MS. Once the method was set-up for bovine hair, it was successfully validated according to Decision 657/2002/CE on three analytes, with satisfying performances. Moreover the applicability of the method was tested on human hair in a one-day validation with reasonable performances. This method could be a useful tool to evaluate natural feed contamination or detect illegal use of α- zearalanol in bovines and to perform a first inventory of the occurrence of ...

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A second implant of Ralgro or Synovex in mid-summer may add another 10 to 15 pounds of gain per head for steers and heifers grazing Missouri pastures.. Missouri field trials used 19 herds with 900 head of steers to compare one initial implant of Ralgro or Synovex with a second application of the same implant 70 to 100 days later for summer grazing periods of 140 to 200 days.. A reimplant of Ralgro increased the average gain per head by 9.5 pounds in 11 trials averaging 166 days. The second implant of Synovex increased average total gain per head by 6.3 pounds in eight trials averaging 181 days. In five trials, where average daily rate of gain ranged from 1.23 to 2.29 pounds after the second implant of Synovex, total gain per head was increased by 14.82 pounds.. These trials indicate that when average daily gain is kept above 1.25 pounds in late summer with good pastures or supplemental grain feeding, a second implant of Ralgro or Synovex can add 15 pounds or more total gain to steers grazing ...

OBJECTIVES: /st, A functional polymorphism leading to reduced levels of growth and differentiation factor 5 (GDF5) was recently identified as a susceptibility factor for osteoarthritis. The authors studied the potential mechanisms of GDF5 involvement in osteoarthritis using haploinsufficient Gdf5(Bp-J/+) mice. METHODS: /st, Gdf5(Bp-J/+) mice were challenged in the collagenase-induced arthritis model, the medial meniscus destabilisation model, the papain-induced arthritis model and a treadmill running model. Bone density and subchondral bone parameters were determined using dual energy x-ray absorptiometry and peripheral quantitative CT. Additional in-vitro and ex-vivo analyses studied cartilage metabolism, gait and collagen characteristics. RESULTS: /st, Gdf5(Bp-J/+) mice appeared phenotypically normal. No difference in osteoarthritis severity was found in the different models, with the exception of increased synovial hyperplasia in the joints of Gdf5(Bp-J/+) mice in the treadmill model. ...

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The athlete challenged the doping charge and was afforded the opportunity to seek legal counsel to present his case and version of events to the Independent Doping Tribunal Panel, South Africas Institute for Drug-free Sport said in a statement.. The panel reached a decision first on the merits of the case where they found the player guilty of a doping offence.. The Zeranol positive case was his third positive dope test during his playing career within a ten-year period, however the players first positive case was ruled a no fault decision and he then received a reprimand.. That positive test was at the end of the Springboks year-end tour to Europe in 2010.. The panel had to refer to sports jurisprudence to determine the appropriate sanction which they ruled to be the players second doping offence, it added.. Ralepelle, who last played for the Sharks in 2019, has 21 days to appeal.. © AAP. ...

Forty-nine Simmental X Hereford and Hereford calves (24 bulls and 25 steers) were used to study the effect of Ralgro on growth, carcass traits, sex drive, sperm production, and development of sex organs. Approximately half ...

LIST OF ALL EURO-DIAGNOSTICA PRODUCTS AND DATA SHEETS ». Zeranol is a xenobiotic anabolic agent with estrogenic growth promoting activities, which is legally used in several countries. However, in the EC the anabolics have been banned since 1986. Because of close structural resemblance the antibody cross-reacts with both zeranol and its main metabolites and with the mycotoxin zearalenone and its main metabolites. The Zeranol-EIA is recommended for the screening of urine samples on the presence of zeranol (100%), taleranol (133%), zearalanone (100%), a. -zearalenol (90%), zearalenone (75%) and ß. -zearalenol (70%). The stilbenes (diethylstilbestrol, hexestrol and dienestrol), 17ß-oestradiol, progesterone and testosterone show a cross-reactivity of less than 0.01%. With this Zeranol-EIA a detection limit of 0.1 ng/ml urine can be obtained.. ...

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article{680923, abstract = {A high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) was developed and validated for the detection of zearalenone (ZON), alpha-zearalenon (alpha-ZOL) and beta-zearalenol (beta-ZOL) in in vitro biological samples. Furthermore, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the detection of ZON, alpha-ZOL, beta-ZOL, alpha-zearalanol (alpha-ZAL) and beta-zearalanol (beta-ZAL) in in vitro biological samples. Zearalanone (ZAN) was used as internal standard in both methods. The limit of detection/limit of quantitation (LOD/LOQ) values of ZON, alpha-ZOL and beta-ZOL were 2/7, 2/7 and 4/13 mu g 1(-1), respectively, for the HPLC-FLD method. For the LC-MS/MS method LOD/LOQ values for ZON, alpha-ZOL, beta-ZOL, alpha-ZAL and beta-ZAL were 6/20, 5/17, 4/14, 9/30 and 6/19 mu g 1(-1), respectively. Within-day and between-day precision were less then 11 and 14\%, respectively for the HPLC-FLD ...

Some of the approved drugs are synthetic versions of the natural hormones, such as trenbolone acetate and zeranol. Just like the natural hormone implants, before FDA approved these drugs, FDA required information and/or toxicological testing in

On July 16, 2021, Japans Ministry of Health, Labour and Welfare (MHLW) proposed revisions to Japans maximum residue levels for 11 agricultural chemicals (MCPA, MCPB, Captan, Cyazofamid, Pyriofenone, Fluoxastrobin, Procymidone, Mandestrobin, Cypermethrin, Albendazole, and Zeranol) for various agricultural commodities ...

On July 16, 2021, Japans Ministry of Health, Labour and Welfare (MHLW) proposed revisions to Japans maximum residue levels for 11 agricultural chemicals (MCPA, MCPB, Captan, Cyazofamid, Pyriofenone, Fluoxastrobin, Procymidone, Mandestrobin, Cypermethrin, Albendazole, and Zeranol) for various agricultural commodities ...

2012) in varying importance but not investigated in humans so far to the best of our knowledge. However, due to an average urinary excretion rate of 72% ( Turner et al., 2010) it can be derived that excretion via feces is not the main route in humans. Two recent in vitro studies examined the metabolism of DON and its plant metabolite DON-3-Glc by the human fecal microbiota and found that DON can be. released from its glycosylated form ( DallErta et al., 2013 and Gratz et al., 2013). Zearalenone metabolism was studied in various animals, especially Selleck NVP-BGJ398 in pigs as they are particularly sensitive to associated adverse effects such as decreased fertility. Biotransformation takes place in two major pathways: Hydroxylation forms the phase-I-metabolites α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL), while conjugation of ZEN GW3965 manufacturer and its reduced forms with glucuronic acid and sulfate leads to the formation of typical phase-II-conjugation products. This was recently ...

The objective of this study was to determine the effect of cow breed type on (a) cow serum and colostrum immunoglobulin (IgG1) concentrations and (b) subsequent calf serum IgG1 concentration and zinc sulphate turbidity (ZST) units. Five cow breed types were examined: LF (Limousin × Friesian), LLF (Limousin × (Limousin × Friesian)), L (Limousin), C (Charolais) and SLF (Simmental × (Limousin × Friesian)). Three blood samples were taken by jugular venipuncture from the cows at approximately 90, 60 and 30 days pre partum, at parturition and at 15 days or more post partum and from the calves at 48 (40 to 56) h post partum. Prior to suckling a 20 ml sample of colostrum was obtained. Milk yield was estimated using the weigh-suckleweigh technique. The decrease in serum IgG1 concentration in cows between 90 days pre partum and parturition was greater (P , 0.01) for LF cows than all other breed types, except SLF. There was no difference between LLF, L, C and SLF cows. There was no effect of cow breed ...

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Abstract: AGPs have been a sensitive issue for some time and there has been a developing controversy surrounding the use of antibiotics as growth promoters for food animals. A review by Philip A Thacker tackles this subject.

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Two commercially available progestogen products for cattle, melengestrol acetate (MGA) and norgestomet (SMB) , were evaluated for their ability to induce synchronized estrus in anovulatory ewes. Seasonally anestrous ewes (n=232; determined by blood serum progesterone concentration) of mixed breeding were randomly assigned within broad age groups to one of seven treatments: 1) control (C); 2) MGA only (OMGA); 3) MGA + zeranol (RMGA); 4) MGA + PG-600 (PMGA; 400 IU pregnant mares serum gonadotropin + 200 IU human chorionic gonadotropin in a 5 mL dose); 5) 5MB only (OSMB); 6) 5MB + zeranol (RSMB); and 7) 5MB + PG-600 (PSMB). Beginning 10 d before breeding, OMGA, RMGA, and PMGA ewes were fed .3 mg MGA/d provided through a mixture of shelled com and a commercially prepared pelleted supplement containing MGA. Concomitantly, OSMB, RSMB, and PSMB ewes were given a 3 mg norgestomet implant inserted subcutaneously on the back of the ear. Immediately preceding initiation of the MGA and 5MB treatments, RMGA ...

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Single crystals of magnesium-aluminate spinel were implanted with 170 keV He{sup +} ions to fluences ranging from 1 x 10{sup 16}--1 x 10{sup 21} ions/m{sup 2} at 120 K. The effects of ion implantation were studied using optical absorption spectroscopy, Rutherford Backscattering Spectroscopy and Ion Channeling (RBS/C) and Transmission Electron Microscopy (TEM). In absorption spectra obtained from the implanted samples, growth of an F-center band at 5.3 eV was observed. At the fluence of 3 x 10{sup 20} ions/m{sup 2}, the growth of this band not only ceases but the intensity suddenly decreases. This may be due to formation of a new phase at this fluence. This is partially confirmed by the fact that beginning at this dose, a modulated absorbance becomes apparent in the absorption spectrum of spinel. This effect is caused by formation of a buried layer with refraction index lower than that of an unimplanted sample. RBS/C and TEM measurements show that spinel is not amorphized over the fluence range examined

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The University of Texas Medical Branch(UTMB) project improves outcomes for severely burned children by instituting and evaluating two modifications to traditional rehabilitation: (1) an intensive rehabilitation program including active resistance exercise; (2) long term administration of anabolic agents.. Effectiveness is assessed by comparison with functional outcomes achieved in traditional outpatient rehabilitation programs. Results indicate improvement in strength, endurance and bone density with these modifications. The project also maintains a longitudinal database that includes measures of cardiopulmonary function, growth and maturation, bone density, range of motion, and psychosocial adjustment. ...

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