TY - JOUR. T1 - The nuclear localization of Y-box binding protein-1 correlates with P-glycoprotein expression in diffuse large B cell lymphoma. AU - Zhou, Lei lei. AU - Xu, Wen lin. AU - Qin, Ru juan. AU - Tang, Hua rong. AU - Shen, Hui ling. AU - Shi, Yang. PY - 2007/5/1. Y1 - 2007/5/1. UR - http://www.scopus.com/inward/record.url?scp=77953721719&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=77953721719&partnerID=8YFLogxK. M3 - Article. C2 - 17706141. AN - SCOPUS:77953721719. VL - 36. SP - 329. EP - 330. JO - Chinese Journal of Pathology. JF - Chinese Journal of Pathology. SN - 0529-5807. IS - 5. ER - ...
This review describes the structure and functions of Y-box binding protein 1 ( YB-1) and its homologs. Interactions of YB-1 with DNA, mRNAs, and proteins are considered. Data on the participation of Y
The Y-box-binding protein 1 (YB-1), a member of the cold-shock domain RNA-and DNA-binding protein family, has pleiotropic functions such as regulation of the cell cycle. The aim of this study was to evaluate if YB-1 is a proliferative marker in breast cancer and elucidate potential downstream targets involved in YB-1-mediated cell cycle regulation using RNA interference technology. YB-1 protein expression was evaluated in tissue microarrays of 131 breast invasive ductal carcinomas by immunohistochemistry, while the YB-1 gene expression profile was evaluated in the T-47D, MDA-MB-231, ZR-75-1 and MCF7 breast cancer cell lines. Silencing of the YB-1 gene in T-47D breast cancer cells was performed using siRNA and the effects of down-regulation of YB-1 on cell growth and regulation of the cell cycle were ascertained. A focused panel of 84 genes involved in cell cycle progression was also examined. In tissue microarrays, YB-1 expression was shown to be associated with proliferating cell nuclear ...
Y-box binding protein-1 (YB-1) is the first reported oncogenic transcription factor to induce the tumor-initiating cell (TIC) surface marker CD44 in triple-negative breast cancer (TNBC) cells. In order for CD44 to be induced, YB-1 must be phosphorylated at S102 by p90 ribosomal S6 kinase (RSK). We therefore questioned whether RSK might be a tractable molecular target to eliminate TICs. In support of this idea, injection of MDA-MB-231 cells expressing Flag-YB-1 into mice increased tumor growth as well as enhanced CD44 expression. Despite enrichment for TICs, these cells were sensitive to RSK inhibition when treated ex vivo with BI-D1870. Targeting RSK2 with small interfering RNA (siRNA) or small molecule RSK kinase inhibitors (SL0101 and BI-D1870) blocked TNBC monolayer cell growth by similar to 100%. In a diverse panel of breast tumor cell line models RSK2 siRNA predominantly targeted models of TNBC. RSK2 inhibition decreased CD44 promoter activity, CD44 mRNA, protein expression, and mammosphere ...
Despite advances in treating breast cancer, disease recurrence rates remain high and secondary tumors are often resistant to chemotherapy and incurable. Currently, the treatment for triple-negative breast cancer (TNBC) relies upon conventional chemotherapeutics as there are no targeted therapies available. Although these tumors initially respond well, they paradoxically have the highest relapse rates.. Y-box binding protein-1 (YB-1) is an oncogenic transcription/translation factor abundantly expressed in TNBC (∼70% of patients). It is activated predominantly by phosphorylation via p90 ribosomal S6 kinase (RSK). Once activated it up-regulates the tumor-initiating-cell (TIC) marker CD44 and induces a TIC phenotype[1]. Due to their inherent drug-resistance, TICs survive chemotherapy and go on to drive relapse[2]. We recently identified RSK2 is critical to the survival of TNBC[3]. Inhibiting RSK2 blocks activation of YB-1 and induces apoptosis in TNBC, including CD44+/CD24- cells. Interestingly, ...
Objective: Investigating the role of Y-box binding protein 1 (YB1) in drug resistant advanced kidney cancer.. Background: Renal cell carcinoma (RCC) is the 6th most common malignancy with approximately 1,800 deaths in 2015 and 2.3% annual increase in Canada. Despite the partial or total surgical removal of kidney in patients with localized RCC, metastatic patients are treated with tyrosine kinase inhibitors (TKIs) in a purely palliative approach. However, TKI-resistance (Sunitinib) is developed after a median time of 10-14 months. Therefore, identifying the factor(s) responsible for TKI-resistance development and disease advancement in RCC is imperative. It is now widely recognized that evolutionarily conserved Y-box binding protein 1 (YB1) is essential for cell growth and survival. Upregulation of YB1 in numerous cancer types was found to be positively correlated with tumor growth, metastasis and drug-resistance development. YB1 is also involved in intercellular communication through its ...
In this work, we identified circulating 18 kDa fragments of YB-1 protein (YB-1/p18) in human plasma using immunoblotting with a monoclonal antibody. The presence of YB-1/p18 in plasma samples may indicate malignant disorders of different origin. We found YB-1/p18 in about 80% of patients with advanced carcinomas and hepatic metastases, but in none of healthy volunteers. Potential confounding variables such as acute inflammations, renal or hepatic dysfunction could be excluded in non-cancerous cohorts. In a very well characterized group of 111 patients with chronic liver diseases, YB-1/p18 had a high sensitivity and reasonable specificity to identify patients with malignant tumors, suggesting its clinical potential as a tumor marker for screening high-risk patient populations.. Although many tumor markers are widely used in monitoring cancer patients during therapeutic interventions, lack of sensitivity and specificity preclude the use of most existing markers for the early detection of ...
DNA binding protein A (dbpA) belongs to the Y-box binding protein family, characterized by an 80 amino-acid cold shock domain that imparts DNA-binding activity. It is also known as cold shock domain protein A (CSDA), CSDA1, ZO-1-associated nucleic acid-binding protein (ZONAB), and single-strand DNA-binding protein NF-GMB. DbpA has been reported to bind to the promoter for granulocyte-macrophage colony-stimulating factor (GM-CSF) and act as a repressor of transcription. It also binds to full-length mRNA and small RNAs containing the consensus site UCCAUCA, suggesting a role as a repressor of translation. Mutations in the CSDA gene have been associated with hepatocarcinogenesis.. ...
DNA binding protein A (dbpA) belongs to the Y-box binding protein family, characterized by an 80 amino-acid cold shock domain that imparts DNA-binding activity. It is also known as cold shock domain protein A (CSDA), CSDA1, ZO-1-associated nucleic acid-binding protein (ZONAB), and single-strand DNA-binding protein NF-GMB. DbpA has been reported to bind to the promoter for granulocyte-macrophage colony-stimulating factor (GM-CSF) and act as a repressor of transcription. It also binds to full-length mRNA and small RNAs containing the consensus site UCCAUCA, suggesting a role as a repressor of translation. Mutations in the CSDA gene have been associated with hepatocarcinogenesis.. ...
Complete information for YBX3 gene (Protein Coding), Y-Box Binding Protein 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for YBX1 gene (Protein Coding), Y-Box Binding Protein 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
We have analyzed the clinical relevance of YB-1 expression in breast cancer by evaluating patients treated with postoperative (primarily anthracyclin-containing) chemotherapy and low-risk patients who did not receive any postoperative chemotherapy. In both groups, high YB-1 expression in breast cancer tissue is linked with an unfavorable clinical course of the disease, indicating that YB-1 plays a role in clinical drug resistance as well as tumor aggressiveness. Moreover, not only high YB-1 expression in tumor cells but also high YB-1 expression in peritumoral epithelial cells is associated with poor clinical outcome. This indicates that YB-1 expression in the adjacent epithelial cells reflects a distinct, obviously more aggressive tumor phenotype.. How peritumoral YB-1 expression is brought about is currently unknown, but it is likely that a paracrine effect between breast cancer and adjacent normal tissue is responsible for this phenomenon. Expression of tumorigenesis-associated factors in ...
Lin28 cold-shock domain complex. Computer model showing the structure of a Lin28 cold-shock domain (purple) complexed with the single-stranded DNA (deoxyribonucleic acid) fragment heptathymidine (yellow). - Stock Image C035/5941
Transcript Variant: This variant (4) contains an alternate 5 terminal exon, and lacks an exon in the 3 coding region (causing a frame-shift) compared to variant 1. This results in translation initiation from an alternate start codon, and a shorter isoform (4) with distinct N- and C- termini compared to isoform 1 ...
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The AuthorDB and ArticleDB grouping is used for holding information related to PubMed articles. As the PubMed ID is a unique feature, it is used in this grouping as the primary key. The Author field is set currently to only 64 characters maximum as no current value even approaches that maximum. This field maximum can be adjusted if a value were to supersede this arbitrary default. The Abstract field is set to longtext due to the varying length of article abstracts. Utilizing the MyISAM engine allows for searching of this field for keywords at the lowest level which is preferable to creating a piece of software to accomplish the same task.. GeneDB, GeneSynonymDB, SpeciesDB, BindingSiteDB, and TF_connect_TFBS_DB form the main grouping of tables used for holding gene and binding site information. The TF_connect_TFBS_DB acts as a directory to allow a gene to know what binding sites it has and for a binding site to know what gene it belongs to. The GeneDB table includes a Cell_memo field that is used ...
Previous studies involving knock-out mice have directly implicated NFIA and NFIB in CNS development, in particular in the formation of midline glia (das Neves et al., 1999; Shu et al., 2003; Steele Perkins et al., 2005). Recently, NFIA also was found to be required for gliogenesis within the developing chick spinal cord (Deneen et al., 2006). NFIB also has been directly implicated in hippocampal and pons development (Steele Perkins et al., 2005). However, knowledge of the broader functions and targets for NFI proteins in developing neurons has remained limited. Our cell culture, in situ and Nfi knock-out studies indicate that NFI family members are central transcriptional regulators of CGN development, their function being important for several major aspects of their postmitotic maturation: axon formation in the PMZ, migration, and dendrite formation within the IGL. Furthermore, we demonstrated previously that NFI directly regulates expression of the extrasynaptic neurotransmitter receptor ...
NF-κB作用机制。在此图中,将以Rel与p50蛋白组成的NF-κB异质二聚体为例。当处于激活状态时,NF-κB位于细胞质中且与抑制蛋白IκBα形成复合体。通过内在膜受体的介导,一些胞外信号物质可激活一种称为IκB激酶(IKK)的酶。IKK转而磷酸化IκBα蛋白,这将导致后者的泛素化,使得IκBα从NF-κB上脱离下来,最终IκBα被蛋白酶体所降解。被激活的NF-κB接下来转移到细胞核内,在这里会结合到DNA上被称为反应元件(RE)的特异性序列上。DNA/NF-κB 复合体接下来会招募其它蛋白,如辅激活物与RNA聚合酶,这些蛋白将下游的DNA转录为mRNA并转而被翻译为蛋白质,这些蛋白最终导致细胞功能发生改变[1][2][3] ...
NF-κB signaling was activated in response to LPS stimulation.Notes: (A and B) H7402 and HepG2 cells were treated with LPS (10 μg/mL) for different time. Then
The cold shock domain (CSD) is a nucleic acid binding domain that is widely conserved from bacteria to higher plants and animals. In Escherichia coli, cold shock proteins (CSPs) are composed solely of a CSD and function as RNA chaperones that destabilize RNA secondary structures. Cellular RNAs tend to be folded into unfavorable structures under low temperature conditions, and RNA chaperones resolve these structures, recovering functionality of the RNAs. CSP functions are associated mainly with cold adaptation, but they are also involved in other biological processes under normal growth conditions. Eukaryotic CSD proteins contain auxiliary domains in addition to the CSD and regulate many biological processes such as development and stress tolerance. In plants, it has been demonstrated that CSD proteins play essential roles in acquiring freezing tolerance. In addition, it has been suggested that some plant CSD proteins regulate embryo development, flowering time and fruit development. In this review, we
CSDE1 antibody [N2C1], Internal (cold shock domain containing E1, RNA-binding) for ICC/IF, IHC-P, WB. Anti-CSDE1 pAb (GTX116218) is tested in Human samples. 100% Ab-Assurance.
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Nuclear factor-κB (NF-κB) has been long considered a master regulator of inflammation and immune responses. Additionally, aberrant NF-κB signaling has been linked with carcinogenesis in many types of cancer. In recent years, the study of NF-κB members in NF-κB unrelated pathways provided novel attractive targets for cancer therapy, specifically linked to particular pathologic responses. Here we review specific functions of IκB kinase complexes (IKKs) and IκBs, which have distinctly tumor promoting or suppressing activities in cancer. Understanding how these proteins are regulated in a tumor-related context will provide new opportunities for drug development ...
TY - JOUR. T1 - Etoposide enhances antitumor efficacy of MDR1-driven oncolytic adenovirus through autoupregulation of the MDR1 promoter activity. AU - Su, Bing Hua. AU - Shieh, Gia Shing. AU - Tseng, Yau Lin. AU - Shiau, Ai Li. AU - Wu, Chao Liang. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Conditionally replicating adenoviruses (CRAds), or oncolytic adenoviruses, such as E1B55K-deleted adenovirus, are attractive anticancer agents. However, the therapeutic efficacy of E1B55K-deleted adenovirus for refractory solid tumors has been limited. Environmental stress conditions may induce nuclear accumulation of YB-1, which occurs in multidrug-resistant and adenovirus-infected cancer cells. Overexpression and nuclear localization of YB-1 are associated with poor prognosis and tumor recurrence in various cancers. Nuclear YB-1 transactivates the multidrug resistance 1 (MDR1) genes through the Y-box. Here, we developed a novel E1B55K-deleted adenovirus driven by the MDR1 promoter, designed Ad5GS3. We tested the ...
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题目】白介素受体激活MYD88-ARNO-ARF6级联反应以干扰血管稳定性. 【译文】固有免疫反应对于抵御感染性疾病至关重要。巨噬细胞和其他细胞通过释放细胞因子,如白介素-1β (IL-1β),对感染产生应答。白介素1β (IL-1β)反过来可以激活髓样分化因子88(MYD88)介导的可以引起炎症细胞激活和募集的核转录因子κB (NF-κB)依赖的转录通路。 上皮细胞通常作为炎症细胞移除血液、移入组织的屏障,它们也是炎症反应的重要调控因子。矛盾的是,对于成功免疫防御重要的细胞因子也对上皮细胞-细胞相互作用具有干扰作用,并可以触发屏障功能降低和组织结构分离。这种屏障分离的机制及其与常规NF-κB通路的关系还不十分清楚。本研究表明人体外细胞模型中IL-1β对上皮稳定性的直接、立即及破坏性作用是NF-κB非依赖的,而不是通过小GTP酶ADP核糖基化因子6 ...
BUFFALO, N.Y. - The absence of one copy of a single gene in the brain causes a rare, as-yet-unnamed neurological disorder, according to new research that builds on decades of work by a University at Buffalo biochemist and his colleagues.. First authors on the paper are Ina Schanze, PhD, from the Institute of Human Genetics at University Hospital Magdeburg and Jens Bunt, PhD, of the Queensland Brain Institute of the University of Queensland, Australia.. Co-author Richard M. Gronostajski, PhD, is a professor of biochemistry in the Jacobs School of Medicine and Biomedical Sciences at UB, director of its Genetics, Genomics and Bioinformatics Graduate Program and a researcher at UBs New York State Center of Excellence in Bioinformatics and Life Sciences. He has been studying the family of nuclear factor I (NFI) proteins, which play important roles in the differentiation of stem cells in the brain.. So far, the absence of some of the proteins has been found to cause several rare diseases from birth ...
The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011 ...
Deficiency of Capn4 gene inhibits nuclear factor-κB (NF-κB) protein signaling/inflammation and reduces remodeling after myocardial infarction.: Calpain has been
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