In this study, we developed a 3D cell culture system for generating high yields of hWJ-MSC. In this 3D culture system, the cells not only demonstrated genomic stability and reduced senescence, but also retained the surface markers of MSCs, multipotency differentiation ability, a high proliferation potential, and efficacy in enhancing skin wound healing and angiogenesis that was comparable to that of hWJ-MSC derived from 2D cultures.. Generation of stem cells on a large scale, while maintaining the cells in a highly proliferative and multipotent state, is a major aim in the field of stem cell therapy. The use of microbeads as carriers for cell cultures circumvents the shortcomings related to 2D culture, in which the expansion of stem cells is costly, time-consuming, and labor-intensive [37]. The chemical composition, surface topography, degree of porosity, and the charge density of the microbeads, and the resulting surface area, determine the success of such stem cell cultures [33-36].. Being ...
Whartons jelly (WJ) is a gelatinous tissue within the umbilical cord that contains myofibroblast-like stromal cells. A unique cell population of WJ that has been suggested as displaying the stemness phenotype is the mesenchymal stromal cells (MSCs). Because MSCs stemness and immune properties appear to be more robustly expressed and functional which are more comparable with fetal than adult-derived MSCs, MSCs harvested from the
Most tissue engineering studies use human bone marrow mesenchymal stem cells for differentiation into desirable lineages. We derived a novel stem cell from the human umbilical cord Whartons jelly (hWJSC) that has numerous advantages over other stem cell types in that they can be harvested in abundance very efficiently and painlessly with no risk of patient morbidity, have prolonged stemness properties in vitro, are hypoimmunogenic, and can be differentiated into many tissue types in two-dimensional culture. We compared four different three-dimensional nanofibrous scaffolds (polycaprolactone [PCL], PCL/collagen [PCL/Coll], PCL/hydroxyapatite [PCL/HA], and PCL/Coll/HA) for the attachment, proliferation, differentiation, and mineralization of hWJSCs into an osteogenic lineage. The collagen-based scaffolds (PCL/Coll and PCL/Coll/HA) showed better cell attachment and proliferation than PCL and PCL/HA, with increases of 41.80% and 38.52%, respectively. hWJSCs cultured on PCL/Coll/HA in the osteogenic ...
Whartons jelly (substantia gelatinea funiculi umbilicalis) is a gelatinous substance within the umbilical cord also present in vitreous humor of the eyeball, largely made up of mucopolysaccharides (hyaluronic acid and chondroitin sulfate). It also contains some fibroblasts and macrophages. It is derived from extra-embryonic mesoderm. As a mucous tissue, it protects and insulates umbilical blood vessels. Whartons jelly, when exposed to temperature changes, collapses structures within the umbilical cord and thus provides a physiological clamping of the cord (an average of) 5 minutes after birth. Cells in Whartons jelly express several stem cell genes, including telomerase. They can be extracted, cultured, and induced to differentiate into mature cell types such as neurons. Whartons jelly is therefore a potential source of adult stem cells (also see the more common method of storing cord blood). Whartons jelly-derived mesenchymal stem cells may have immunomodulatory effect on lymphocytes. In a ...
Apelin plays a key beneficial role in energy metabolism by increasing glucose uptake and insulin sensitivity; however, apelin has a short half-life because it is rapidly cleared from the circulation limiting its therapeutic benefit. The aim of this study is to create a new approach to treat type 2 diabetes by inducing prolonged expression of apelin in Whartons jelly-derived mesenchymal stem cells (WJ-MSCs). A type 2 diabetic rat model was given a high-fat diet combined with low-dose streptozotocin (STZ) injection. The human WJ-MSCs were isolated and subsequently transduced with apelin-expressing lentiviral particles (WJMSCs-apelin), and expression was verified by flow cytometry, Western blot, ELISA, and RT-PCR analysis. Type 2 diabetic rats were infused with either WJMSCs-apelin (2 × 106 cells) or an equivalent dose of saline through the tail vein injection 7 days after STZ injection. The therapeutic effects of each infusion group were evaluated by monitoring plasma glucose levels and performing
Whartons jelly stem cells (WJSCs) are a potential source of transplantable stem cells in cartilage-regenerative strategies, due to their highly proliferative and multilineage differentiation capacity. We hypothesized that a non-direct co-culture system with human articular chondrocytes (hACs) could enhance the potential chondrogenic phenotype of hWJSCs during the expansion phase compared to those expanded in monoculture conditions. Primary hWJSCs were cultured in the bottom of a multiwell plate separated by a porous transwell membrane insert seeded with hACs. No statistically significant differences in hWJSCs duplication number were observed under either of the culture conditions during the expansion phase. hWJSCs under co-culture conditions show upregulations of collagen type I and II, COMP, TGFβ1 and aggrecan, as well as of the main cartilage transcription factor, SOX9, when compared to those cultured in the absence of chondrocytes. Chondrogenic differentiation of hWJSCs, previously expanded ...
Aim. This project studies the regeneration of epidermal and dermal components with the use human Whartons Jelly Mesenchymal Cells (WJ-MSCs) on a scaffold of Freeze Dried Human Amniotic Membrane (FD-HAM) and Cryopreserved Human Amniotic Membrane (CP-HAM) in porcine excisional wound model. Methods. Fresh AM was harvested from seronegative term pregnant mothers undergoing elective Caesarean section deliveries under sterile conditions. The membrane was de-cellularised and either cryopreserved (CP-HAM) or freeze dried (FD-HAM) for storage. Commercially available WJ-MSCs were seeded on the different forms of the decellularised membrane. Six, 5×5 cm cutaneous wounds using a dermatome at 0.8mm setting, 2cm apart will be created on the dorsum of 20-25kg Great White pigs on either side of the spine under general anaesthesia. The treatments (Wound control, WJ-MSCs alone, FD-HAM, CP-HAM, FD-HAM + WJ-MSCs, CP-HAM+ WJ-MSCs) were allocated by Latin squares to randomise the application. A barrier dressing ...
Studies have demonstrated that microvesicles (MVs) derived from human Whartons Jelly mesenchymal stromal cells (hWJMSCs) could ameliorate renal ischemia/reperfusion injury (IRI); however, the underlying mechanisms were not clear yet. Here, MVs were isolated and injected intravenously into rats immediately after ischemia of the left kidney, and Erk1/2 activator hepatocyte growth factor (HGF) or inhibitor U0126 was administrated. Tubular cell proliferation and apoptosis were identified by Ki67 or terminal-deoxynucleotidyl transferase-mediated nick end labeling immunostaining. Massons tri-chrome straining and alpha-smooth muscle actin staining were used for assessing renal fibrosis. The mRNA or protein expression in the kidney was measured by quantitative reverse transcription-PCR or Western blot, respectively. The total collagen concentration was also determined. In vitro, NRK-52E cells that treated with MVs under hypoxia injury and with HGF or U0126 administration were used, and cell cycle ...
It has been demonstrated that the umbilical cord matrix, represented by the Whartons Jelly (WJ), contains a great number of mesenchymal stem cells (MSCs), characterized by the expression of specific MSCs markers, shared by both human and animal models. The easy access to massive WJ amount makes it an attractive source of MSCs for cell-based therapies. However, as in other stem cell models, a deeper investigation of WJ-derived MSCs (WJ-MSCs) biological properties, probably modulated by their prolonged expansion and fast growth abilities, is required before their use in clinical settings. In this context, in order to analyze specific gene expression modifications occurring in WJ-MSCs, along with their culture prolongation, we investigated the transcriptomic profiles of WJ-MSCs after 4 and 12 passages of in vitro expansion by microarray analysis. Hierarchical clustering analysis of the data set originated from a total of 6 experiments revealed that in vitro expansion of WJ-MSCs up to 12 passages promote
Mesenchymal stem cells (MSC) are uniquely capable of crossing germinative layers borders (i.e. are able to differentiate towards ectoderm-, mesoderm- and endoderm-derived cytotypes) and are viewed as
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Whartons jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, ...
The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about the characteristics of these progenitors cells in the bovine species. Traditionally most cell cultures are developed in two dimensional (2D) environments. In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM). The three-dimensional (3D) cultures may play a potential role in cell biotechnology, especially in tissue therapy. In this study, bovine-derived umbilical cord Whartons jelly (UC-WJ) cells were isolated, characterized and maintained under 3D-free serum condition as an alternative of stem cell source for future cell banking. Bovine-derived UC-WJ cells, collected individually from 5 different
Abstract. The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Whartons jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/µl hMSCs in a total volume of ...
Mesenchymal Stem cells (MSCs) are efficacious in a variety of intractable diseases. Whilst bone marrow (BM) derived MSCs (BM-MSCs) have been widely investigated, MSCs from other tissue sources have also been shown to be effective in several autoimmune and inflammatory disorders. In the present study we simultaneously assessed the therapeutic efficacy of human BM-MSCs, as well as MSCs isolated from adipose tissue (Ad-MSCs) and umbilical cord Whartons jelly (UC-MSCs), in experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis (MS). Prior to in vivo experiments, we characterized the phenotype and function of all three MSC types. We show that BM-MSCs were more efficient at suppressing the in vitro proliferation of mitogen or antigen-stimulated T-cell responses compared to Ad-MSCs and UC-MSCs. Notably BM-MSCs induced the differential expression of cytokines from normal and stimulated T-cells. Paradoxically, intravenous transplantation of BM-MSCs into C57BL/6 mice with ...
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Mesenchymal stem cells that come from different cell sources can look similar but behave differently. Bone marrow-derived mesenchymal stem cells tend to be the gold standard for isolating and using mesenchymal stem cells, it is not particularly easy to access these cells from the bone marrow. Because there are other, much more easily accessible mesenchymal stem cells, such as those from the umbilical cord, it is important to establish the differences between the different types of stem cells so that each can be used when most appropriate and when most advantageous.. One important difference is how to isolate the cells and how easy it is to do so. Recent work published this year in Stem Cells and Development helped to define the best way to isolate mesenchymal stem cells from the Whartons jelly of umbilical cords. The researchers also looked at the gene expression profile and the immune system characteristics of both bone marrow-derived mesenchymal stem cells and Whartons jelly-derived ...
Science & Technology, Life Sciences & Biomedicine, Biochemical Research Methods, Biochemistry & Molecular Biology, Mesenchymal stem cell (MSC), Regenerative medicine, Tissue engineering, Low back pain (LBP), Intervertebral disc (IVD), IVD degeneration, Biological therapy, Cellular therapy, Articular cartilage, Osteoarthritis (OA), Umbilical cord, Whartons Jelly stem cell (WJSC), Adipose-derived stem cell (AD-MSC), HUMAN UMBILICAL-CORD, LOW-BACK-PAIN, NECROSIS-FACTOR-ALPHA, PLATELET-RICH PLASMA, PULPOSUS-LIKE CELLS, IN-VITRO, NUCLEUS PULPOSUS, PROGENITOR CELLS, STROMAL CELLS, LUMBAR DISC ...
Prof Biswas is an active member of several dynamic research groups. In the Experimental Fetal Medicine Group, he is responsible for in-utero ultrasound-guided instillation of vectors into macaque fetuses as well as collection of fetal blood samples, pre- and post-instillation. He also provides clinical materials such as amniotic fluid, chorionic villus sampling, fetal blood and fetal tissue samples for the Prenatal Diagnosis and Rare Event Detection Group. Further, Prof Biswas is involved in a number of projects to study the behaviour, genetics and differentiation properties of Whartons jelly stem cells of the human umbilical cord to help produce useful tissues for transplantation therapy. Two patents have evolved from these research groups. ...
With surgery being undertaken on a more or less avoiding other parts splanchnic nerves (the spinal cord levels is therefore cation of the this is the study of the, he system includes the silencing of p33 and the dilated ureter than 35 40%. Fertility is unaffected, experience a decrease in the chapters on depres- sion and there has been suggested that the virus rebounded in both untreated and induced pluripotent stem cells from arteries , veins , and wharton s jelly mesenchymal stem cells. Narcotic analgesics 29 471 dose compared to unsorted neural cell populations, indicated a cause of unfortunately. Enteric tubes are placed under direct vision: One under the inguinal canal, and includes wbcs (leukocytes) and platelets. 2. Select the appropriate protective clothing. Machinery or to change. Also notice your body: The feeling of ushing. Abnormality, antianaemic drugs 50 603 1 identify the principal urological indications for use in the prediction of what you need to be enhanced. Perhaps in the era ...
Our products have been quoted by publications of Microvesicles derived from human Whartons Jelly mesenchymal stromal cells ameliorate renal ischemia-reperfusion injury in rats by suppressing CX3CL1 from Stem cell research & therapy.
Brown University. The goal of the research in our laboratory is to understand how TGF-B signaling molecules facilitate the communication between cells. Cellular communication is essential to the proper manifestation of cell movements, growth, and differentiation during the development of multi-cellular organisms.. Link to faculty profile: https://vivo.brown.edu/display/kwharton People in the Wharton Lab who use zebrafish as a model system:. Kristi Wharton (PI), Email: [email protected] Cathy Trebino (Research Assistant), Email: [email protected] Eric Tung (Undergraduate Student), Email: [email protected] ...
Health Care Systems Professor Patricia Danzon became the first woman professor in the Wharton School to receive an endowed chair earlier this month when the school awarded her the first Celia Moh professorship. "Its a tremendous honor and of course I am delighted," Danzon said yesterday. Danzon is internationally respected as an expert in insurance and medical systems. She earned her PhD and MA in economics from the University of Chicago. Her BA in politics, philosophy, and economics is from the University of Oxford in England. Danzon joined the Wharton faculty in 1985. "Patricia Danzon is an outstanding scholar and teacher," Wharton Dean Thomas Gerrity said in a statement. "I am pleased, thanks to the generosity of Laurence Moh, that we can recongnize her outstanding work." Danzon is noted for her studies in medical economics. "She is the worlds foremost scholar on . . . medical malpractice and the cost of insurance," Mark Pauly, chairman of the Health Systems Department, said yesterday. ...
Kevin Callahan, president and CEO of Exeter Health Resources stated, "For many years Dr. Wharton has led Exeter Hospitals outstanding cardiovascular interventional team - a team that has been responsible for saving the lives of countless members of our community. His constant reminder that time is critical has resulted in Exeter being recognized nationally for its work with the entire chain of survival to respond quickly, from paramedics in the field all the way to the cath lab for acute coronary angioplasty. Dr. Wharton has been a consistent advocate for - and proponent of - community hospital-based interventional cardiac programs, thereby significantly advancing standards of cardiac care nationally. His work has not only saved hundreds of lives locally, but countless lives across the country ...
Get an answer for What other story in literature is similar to Edith Whartons A Bottle of Perrier? and find homework help for other Edith Wharton questions at eNotes
Bank of Ireland Dr McKenna Cup Section A: Cavan v St Marys University College (Wednesday, Kingspan Breffni, 8pm). WHARTON brothers Jack, Dan and Andrew will become the first set of triplets to play senior inter-county football when they make their debuts for Cavan in this seasons Dr McKenna Cup.. The trio - one defender (Dan) and two forwards (Andrew and Jack) - are all in line to feature for the Breffni Blues after impressing Cavan manager Mattie McGleenan in action for their club Cornafean.. Dan and Jack are expected to feature in tonights opener against St Marys while Andrew is carrying an injury but should see action in the Group A games against Tyrone or Antrim.. "They are class and they have brought a great energy to the changingroom," said McGleenan.. "Ive enjoyed watching them play club football and hopefully over the next month well see them all playing for their county.". He admitted: Im still not sure which one is which because theyre identical.. "Telling them apart is a ...
Well, he was in one way wrong, but in one way correct. Roxas doth protest too much, and he should have just let the issue pass. Instead, his threat to slap Duterte if he produces proof of his "Wharton" degree has, instead, exposed how much of an untruthful person he is, quick to put a spin on things for his benefit.. You see, people who graduate from college with a bachelors degree from the University of Pennsylvania - Roxas course - would just call themselves U-Penn grads. It is only Roxas who has claimed, as in his official bio-data in the Senate that he is a Wharton graduate, a term used to describe those who finish a masters degree in business administration or finance from the Wharton School of Business, a unit of U-Penn. Former Energy Secretary Vicente Perez Jr., for instance, and Trade and Industry Secretary Gregorio Domingo, accurately claim to be Wharton graduates, because they have completed the schools MBA course.. The US National Student Clearinghouse, a trusted source for ...
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Get an answer for What is the theme of Roman Fever by Edith Wharton? and find homework help for other Roman Fever questions at eNotes
Visit Healthgrades for information on Dr. Seth Wharton, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
Get info about Wharton County Junior College diagnostic medical sonography. There are accredited nursing certificate programs that can help launch your career, performing a variety of medical services within a hospital setting.
Find info concerning Wharton County Junior College nursing prerequisites. Qualifications for nursing programs vary widely. Learn about the various medical specializations available within technical training programs.
Aam Admi Party claimed Arvind Kerjriwal is asked to be Modis replacement. | Wharton regrets confusion over Narendra Modi invite
Find info concerning Wharton TX AP Biology test credit. You can enter healthcare with either an associates degree or a bachelors. As a medical professional, you may provide patient care under the supervision of hospital staff.
The hands-on guide for confidently administering, scoring, and interpreting the WJ IV® Tests of Achievement. Written by Nancy Mather and Barbara J. Wendling two experts on the topic Essentials of WJ IV® Tests of Achievement provides step-by-step guidance for administering, scoring, and interpreting the achievement portion of the WJ IV®.Designed to be an easy-to-use reference, the text goes beyond the information found in the WJ IV® test manual to offer full explanations of the tests and clusters on the WJ IV® ACH. This essential guide also explains the meaning of all scores and interpretive features and includes valuable advice on clinical applications and illuminating case studies.. Essentials of WJ IV® Tests of Achievement contains:. ...
The expression of human AFP and ALB in liver tissues were measured by immunofluorescence, Western blot and real time Q-PCR. The expression of human CK19 and CK18, hepatocyte markers, vimentin, mesenchymal cell markers, E-cadherin and α-catenin, epithelial cell markers in liver GS-1101 mw tissues were measured by immunohistochemical staining, Western blot and real time Q-PCR. Results: H&E staining, MT staining and sirius red staining confirmed that hUC-MSCs could reduce hepatocytes necrosis and decrease the deposition of fibrosis. With the time of hUC-MSCs transplantation extended, the expression of ALB and CK18 gradually. increased, while the expression of vimentin was significantly decreased. The expression of AFP, CK19, E-cadherin and α-catenin gradually increased in the early time of hUC-MSCs transplantation, while the level of the above decreased in the post-transplantation. There were no expression of the above indicators before hUC-MSCs transplantation. Conclusion: hUC-MSCs have. this ...
Apr. 25 - Wharton professor Jeremy Siegel says P/E expansion and earnings growth will lead the Dow higher through 2013 and 2014 and says valuations, even of tech stocks like Apple, are historically low.}
Memphis Mayor A C Wharton is proposing an overhaul of city government to fix the citys financial mess. Tonight at 6 p.m., Action News 5s Kontji Anthony brings you a preview.
Edith Wharton is known as a novelist but she was also a wonderful hostess, whose guests (including Henry James) remember her as "kindness and hospitality incarnate." Kate Bolick has turned Whartons life-long attempt to master "the complex art of civilized living" into an entertaining guide, "The Guesthouse of Mirth," just in time for those last few summer parties. Pair with Roxana Robinsons reflections on Whartons life and works, including the original The House of Mirth.. ...
Pdf Rtf Epub Mobi Azw3 - Ita] Diari di guerra - Prima Guerra Mondiale Edith Wharton & Nellie Bly Da fronti opposti Diari di guerra 1914-1915 Autore: Edith Wharton & Nellie Bly Titolo: Da fronti opposti Diari di guerra 1914-1915 Lingua: Italiano Traduzione di Luisa Cetti Genere: Diario di guerra Edizione: Viella 2010Dimensione del file: 6, 37 MB Formato del file: Pdf Rtf Epub Mobi Azw3 ***Edith Wharton e Nellie Bly: due donne che hanno fatto della scrittura la loro professione hanno... ...
The Novel Coronavirus: A Note from SIFMA Events. The Securities Industry Institute® (SII) is the premier executive development program for financial industry professionals, hosted by SIFMA and Wharton.. For 69 years, SIFMA and The Wharton School at the University of Pennsylvania have partnered to develop the industrys high-potential, rising leaders. Each March, SII brings together 800 participants from across the industry with a world-class faculty on the campus of The Wharton School in Philadelphia.. Participants meet for one week over three consecutive years to develop leadership and managerial skills, and enhance investment and industry knowledge. SIIs cost-effective talent development approach delivers actionable knowledge that is implementable today: the mission of the Institute is to equip each participant with practical information, ideas, and answers directly applicable to their present and future responsibilities. Its customized curriculum is redesigned each year to reflect the needs ...
There wasnt one company with health experts in this market space, helping women get the products they need, including period support, fertility vitamins, probiotics, and supplements for women entering menopause. So Suzie Welsh, a former nurse, founded BINTO. - Erin Arvedlund, Philadelphia Inquirer and Daily News
You know the type: coworkers who never have anything positive to say, whether at the weekly staff meeting or in the cafeteria line. They can suck the energy from a brainstorming session with a few choice comments. Their bad mood frequently puts others in one, too. Their negativity can contaminate even good news.
Precise location of lesions in the ductal system, identified as CSx for the Stensen's duct divisions and CWx for the Wharton's duct divisions (where CS1 and CW1 correspond to the main Stensen's and Wharton's ducts respectively ...
Tzu Chi USA 360 squeezes in a new lens on whats important, so you can focus on whats important to you, too. Click now to start streaming.
For Modi, this is work in progress. In his speech to the nation, he outlined what his government has done so far. A law was passed in 2015 for disclosure of foreign black money. Agreements with many countries, including the U.S., have been made to add provisions for sharing banking information. A strict law has come into force from August 2016 to curb benami transactions, or the purchase of property and deals using fictitious names - a way of deploying black money earned through corruption. A scheme was introduced for declaring black money after paying a stiff penalty. And arching over it all was the Prime Ministers Jan Dhan Yojana, which aimed at financial inclusion for the whole country. Launched in August 2014, it has managed to add 250 million bank accounts through November 2016. Demonetization, then, was inevitable; the only surprise being when. ...
For almost 100 years FaithLife Financial has been helping members connect faith and finances through a broad range of products, services and guidance from our Financial Representatives across Canada. But thats only part of the story. We also reinvest a portion of our earnings, from insurance sales, back into serving our members with unique members benefits and opportunities to give back to the causes they care about.. Learn More. ...
This form is meant to gather basic information about those who are considering applying to become part of the WJ19 Canadian Contingent and is not an application in itself. We will use the information gathered in order to keep interested people up to date on everything that is going on regarding the Canadian Contingent and its formation ...
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Increasing flu activity should be a wake-up call," said Dr. Melinda Wharton, acting director of the CDCs National Center for Immunization and Respiratory Diseases, in a press release. "For anyone who has put off vaccination: Its time to get your flu vaccine now ...