TY - JOUR. T1 - Deletions in herpes simplex virus glycoprotein D define nonessential and essential domains. AU - Feenstra, Veronica. AU - Hodaie, Mojgan. AU - Johnson, David. PY - 1990. Y1 - 1990. N2 - Herpes simplex virus glycoprotein D (gD) is a major component of the virion envelope and infected cell membranes and is essential for virus entry into cells. We have recently shown that gD interacts with a limited number of cell surface receptors which are required for virus penetration into cells. To define domains of gD which are required for aspects of virus replication including receptor binding, deletion mutations of 5 to 14 amino acids were constructed by using oligonucleotide-directed mutagenesis. Plasmids containing mutant genes for gD were assayed for the ability to rescue a recombinant virus, F-gDβ, in which β-galactosidase sequences replace gD-coding sequences. Effects on global folding and posttranslational processing of the molecules were assessed by using a panel of monoclonal ...
gB, but not gD or gH/gL, binds to liposomes.Our laboratory previously showed that HSV virions bind to liposomes when incubated in the presence of a soluble form of the gD receptor, HVEM, at pH 5.0 (55). Second, gB730t, but not soluble forms of gD or gC, can bind to cholesterol-rich lipid rafts (10). Together, these data suggest that HSV may interact with lipids through gB. In support of this hypothesis, mutations in the fusion loops of two class II fusion proteins from flavivirus (2) and Semliki Forest virus (35) impair the ability of these proteins to associate with liposomes. Therefore, we theorized that if gB inserts into target membranes via the putative fusion loops, gB730t should associate and float with liposomes, while the soluble forms of the fusion loop mutants should be impaired in this association.. To test this hypothesis, purified soluble gB, gD, and gH/gL were each incubated with two different compositions of liposomes, i.e., PC only or PC/C, at 37°C for 1 h. We then added KCl to ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
TY - JOUR. T1 - Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells. AU - Inudoh, M.. AU - Nyunoya, H.. AU - Tanaka, T.. AU - Hijikata, M.. AU - Kato, N.. AU - Shimotohno, K.. PY - 1996/12/1. Y1 - 1996/12/1. N2 - A putative second envelope glycoprotein (E2) of hepatitis C virus (HCV) was constitutively produced in a Chinese hamster ovary cell line stably transformed with a plasmid expressing E2 protein under the control of an exogenous promoter and a signal sequence. E2 protein that lacked part of the C-terminal hydrophobic region was glycosylated with high-mannose type oligosaccharides and retained in the cells. On the other hand, E2 protein lacking the entire C-terminal hydrophobic region was glycosylated with complex type oligosaccharides (complex form) and excreted into the culture medium. Immunoreactivity of the high-mannose and complex forms of E2 proteins against sera from HCV infected patients were analyzed. We found that the antigenicity of the ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
This invention provides an isolated nucleic acid which comprises a nucleotide segment having a sequence encoding a viral envelope protein comprising a viral surface protein and a corresponding viral transmembrane protein wherein the viral envelope protein contains one or more mutations in amino acid sequence that enhance the stability of the complex formed between the viral surface protein and transmembrane protein. This invention also provides a viral envelope protein comprising a viral surface protein and a corresponding viral transmembrane protein wherein the viral envelope protein contains one or more mutations in amino acid sequence that enhance the stability of the complex formed between the viral surface protein and transmembrane protein. This invention further provides methods of treating HIV-1 infection.
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The coronavirus E protein is a small membrane protein that has an important role in the assembly of virions. Recent studies have indicated that the E protein has functions during infection beyond assembly, including in virus egress and in the host stress response. Additionally, the E protein has ion channel activity, interacts with host proteins, and may have multiple membrane topologies. The goal of this review is to highlight the properties and functions of the E protein, and speculate on how they may be related.
Mesalam, Ahmed Atef and Desombere, Isabelle and Farhoudi, Ali and Van Houtte, Freya and Verhoye, Lieven and Ball, Jonathan and Dubuisson, Jean and Foung, Steven K.H. and Patel, Arvind H. and Persson, Mats A.A. and Leroux-Roels, Geert and Meuleman, Philip (2018) Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1. Virology, 514 . pp. 30-41. ISSN 0042-6822 Desombere, Isabelle and Mesalam, Ahmed Atef and Urbanowicz, Richard A. and Van Houtte, Freya and Verhoye, Lieven and Keck, Zhen-Yong and Farhoudi, Ali and Vercauteren, Koen and Weening, Karin E. and Baumert, Thomas F. and Patel, Arvind H. and Foung, Steven K.H. and Ball, Jonathan and Leroux-Roels, Geert and Meuleman, Philip (2017) A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo. Antiviral Research, 148 . pp. 53-64. ISSN 1872-9096 Khera, Tanvi and Todt, Daniel and Vercauteren, Koen and ...
Shop Large envelope protein ELISA Kit, Recombinant Protein and Large envelope protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Stamataki, Zania and Coates, Stephen and Evans, Matthew J and Wininger, Mark and Crawford, Kevin and Dong, Christine and Fong, Yiu-Lian and Chien, David and Abrignani, Sergio and Balfe, Peter and Rice, Charles M and McKeating, Jane A and Houghton, Michael (2007) Hepatitis C virus envelope glycoprotein immunization of rodents elicits cross-reactive neutralizing antibodies. Vaccine, 25 (45). pp. 7773-84. ISSN 0264-410X. ...
Stamataki, Zania and Coates, Stephen and Evans, Matthew J and Wininger, Mark and Crawford, Kevin and Dong, Christine and Fong, Yiu-Lian and Chien, David and Abrignani, Sergio and Balfe, Peter and Rice, Charles M and McKeating, Jane A and Houghton, Michael (2007) Hepatitis C virus envelope glycoprotein immunization of rodents elicits cross-reactive neutralizing antibodies. Vaccine, 25 (45). pp. 7773-84. ISSN 0264-410X. ...
FUNCTION: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties. TISSUE SPECIFICITY: Low expression in placenta and testis. DOMAIN: Contains the CKS-17 immunosuppressive domain present in many retroviral envelope proteins. As a synthetic peptide, it inhibits immune function in vitro and in vivo (By similarity ...
Retroviral (MLV-based) and lentiviral (HIV-1-based) vectors were harvested once at 1.5 days and again at 2.5 days post-transfection. Retroviral genomic particles pseudotyped with VSVGs (gMLV-VSVG) were produced at a higher titer during the first harvest than during the second harvest (Fig. 1A). However, more retroviral transducing particles (functional ones, tMLV-VSVG) were produced during the second harvest than during the first harvest. The number of gMLV-VSVG particles required for the transduction of a single cell (genomic to transducing particle ratio) can be calculated to be 2,200 for the first harvest, but 1,200 for the second harvest. This result indicates that gMLV-VSVG particles assembled at later time points post-transfection are more infectious than the ones assembled earlier.. In contrast, both lentiviral genomic and transducing particles pseudotyped with VSVGs (gHIV1-VSVG and tHIV1-VSVG, respectively) were produced at a higher titer during the first harvest than during the second ...
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.
RSV Glycoprotein G research reagents are researched and produced in house with premium quality at affordable price. Bulk in stock.
With enhanced promotor, RSV Glycoprotein G cDNA ORF Clone, RSV in pCMV3-C-OFPSpark is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, RSV Glycoprotein G cDNA ORF Clone, RSV in pCMV3-untagged is expression-ready, and confirmed by full-length sequence & expression validation
TY - JOUR. T1 - Different antibody response to a neutralizing epitope of human cytomegalovirus glycoprotein B among seropositive individuals. AU - Ayata, Minoru. AU - Sugano, Tohru. AU - Murayama, Tsugiya. AU - Sakamuro, Daitoku. AU - Takegami, Tsutomu. AU - Matsumoto, Yoh‐Ichi ‐I. AU - Furukawa, Toru. PY - 1994/8. Y1 - 1994/8. N2 - The amino‐terminal portion of human cytomeg‐alovirus glycoprotein B (HCMV‐gB) was expressed as a fusion protein to analyze the neutralizing epitope recognized by human monoclonal antibody C23 and the humoral immune response to this epitope. The linear neutralizing epitope was further localized to the pep‐tide within 17 amino acids (position 68‐84) which were conserved between two HCMV laboratory strains. Ten out of 17 HCMV‐seropositive human sera contained the antibody against this epitope. Although seven sera were negative for reacting with the fusion protein, the viruses isolated from the same patients retained the epitope. The immunogenicity of the ...
The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely replaced by the G glycoprotein of vesicular stomatitis virus. Such vectors can be concentrated by ultracentrifugation to titers , 10(9) colony-forming units/ml and can infect cells, such as hamster and fish cell lines, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein. The ability to concentrate vesicular stomatitis virus G glycoprotein pseudotyped vectors will facilitate gene therapy model studies and other gene transfer experiments that require direct delivery of vectors in vivo. The availability of these pseudotyped vectors will also facilitate genetic studies in nonmammalian species, including the important zebrafish developmental system, through the efficient ...
TY - JOUR. T1 - Expression of murine leukemia virus envelope glycoprotein gp69/71 on mouse thymocytes. Evidence for two structural variants distinguished by presence vs absence of G(ix) antigen. AU - Tung, J. S.. AU - Fleissner, E.. AU - Vitetta, E. S.. AU - Boyse, E. A.. PY - 1975. Y1 - 1975. N2 - Thymocytes of several mouse strains were tested for expression of the gp69/71 envelope component of murine leukemia virus by surface iodination (125I), followed by immunoprecipitation and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. These strains included two congenic lines differing from their partner stocks with respect to expression of G(IX) antigen demonstrable in the cytotoxicity assay. It is concluded that two structural variants of gp69/71 can be expressed on mouse thymocytes, that these are distinguishable by a small difference in mobility in SDS gels, that one carries G(IX) antigen and the other not, that they are coded, or their expression is regulated, by different ...
Background & Objectives: Interaction of cytomegalovirus glycoprotein B with toll-like receptors of dendritic cells leads to early signaling and innate immune responses. The aim of this study is to evaluate the effects of cytomegalovirus glycoprotein B on the maturation and function of monocyte-derived dendritic cells in treated groups in comparison with control ...
TY - JOUR. T1 - Vesicular stomatitis virus glycoprotein contains a dominant cytoplasmic basolateral sorting signal critically dependent upon a tyrosine. AU - Thomas, DNette C.. AU - Brewer, Colleen B.. AU - Roth, Michael G.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - To investigate the contribution of the cytoplasmic domain of the vesicular stomatitis virus G glycoprotein to its basolateral expression in polarized epithelial cells, chimeric proteins containing the external and transmembrane domains of an apically targeted protein, the influenza virus hemagglutinin (HA), and either the G cytoplasmic domain or an unrelated cytoplasmic sequence, were introduced into Madin-Darby canine kidney (MDCK) cells. Addition of the cytoplasmic tail of G to a truncated HA resulted in delivery of greater than 95% of the chimeric protein to the basolateral cell surface, indicating that the G cytoplasmic domain contains a dominant basolateral sorting signal. A similar chimera, containing the cytoplasmic tail of herpes ...
In this study, we show that posttranslational folding of Vesicular Stomatitis virus G protein subunits can involve noncovalent, multimeric complexes as transient intermediates. The complexes are heterogeneous in size (4-21S20,W), contain several G glycopolypeptides, and are associated with BiP/GRP78. The newly synthesized, partially intrachain disulfide-bonded G proteins enter these complexes immediately after chain termination, and are released 1-4 min later as fully oxidized, trimerization-competent monomers. These monomers are properly folded, judging by their binding of conformation-specific mAbs. When the G protein is translated in the presence of DTT, it remains reduced, largely unfolded and aggregated in the ER, but it can fold successfully when the DTT is removed. In this case, contrary to normal folding, the aggregates become transiently disulfide cross-linked. We also demonstrated that the fidelity of the folding process is dependent on metabolic energy. Finally, we established that ...
Glycoprotein D (gD) of HSV has been shown to be a potent immunogen. To analyze the T cell antigenic determinants on gD, a series of 28 overlapping 20-mer peptides that span the extracellular portion of gD-1 were examined for their ability to stimulate T cells from rgD-1 or infectious HSV-1-primed H-2d mice in vitro. rgD-1-primed cells responded exclusively to peptide 241-260, the immunodominant determinant of gD in H-2d mice. In contrast, infectious HSV-primed T cells were shown to respond to 17 (and up to 22) of 28 synthetic gD peptides. These results indicate an extensive diversity in the T cell repertoire to gD in H-2d mice with T cells directed to a broad array of peptide determinants being recruited during the acute phase of an HSV infection. ...
Structure of a trimeric variant of the Epstein-Barr virus glycoprotein B. - Marija Backovic, Richard Longnecker, Theodore S Jardetzky
Previous work with HSV and the related alphaherpesviruses PrV and varicella-zoster virus has clearly demonstrated that gE-gI mediates or facilitates cell-to-cell spread, especially in solid tissues such as epithelium, in the nervous system, and with certain cultured cells, e.g., normal fibroblasts, epithelial cells, and neurons, cells that form extensive cell junctions (2, 24, 25). Other HSV glycoproteins function similarly in cell-to-cell spread, but unlike these glycoproteins, gE-gI functions in cell-to-cell spread but not in the entry of extracellular virions. Therefore, interactions between gE-gI and cell junctions may provide molecular details of how a herpesvirus moves directly from cell to cell.. When gE-gI was expressed in HEC-1A cells or in other epithelial cells by recombinant Ad vectors, the protein accumulated specifically along the lateral surfaces of cells and was not found on either the apical or basal surfaces or at tight junctions. Sorting of gE-gI to the basolateral surfaces of ...
Human cytomegalovirus (HCMV) infections are life-threating to people with a compromised or immature immune system. Upon adhesion, fusion of the virus envelope with the host cell is initiated. In this step, the viral glycoprotein gB is considered to represent the major fusogen. Here, we present for the first time structural data on the binding of an anti-herpes virus antibody and describe the atomic interactions between the antigenic domain Dom-II of HCMV gB and the Fab fragment of the human antibody SM5-1. The crystal structure shows that SM5-1 binds Dom-II almost exclusively via only two CDRs, namely light chain CDR L1 and a 22-residue-long heavy chain CDR H3. Two contiguous segments of Dom-II are targeted by SM5-1, and the combining site includes a hydrophobic pocket on the Dom-II surface that is only partially filled by CDR H3 residues. SM5-1 belongs to a series of sequence-homologous anti-HCMV gB monoclonal antibodies that were isolated from the same donor at a single time point and that ...
Dr Butters works in the Glycobiology Institute which is headed by Professor Raymond Dwek. His expertise in glycoprotein biology led to him being contacted by Dr Rossignol.. Dr Rossignol approached our group to see if we could help him find a mechanism of action for thiazolides. If we understand the mechanism better, then well be able to improve drug design, explains Dr Butters.. His group works with small molecules that interfere with the cellular processes responsible for glycoprotein production. He is trying to develop these into drugs against the hepatitis C virus which also uses a viral envelope glycoprotein.. If more can be uncovered about how thiazolides work, this may also open up the possibility of using them in combination with compounds Dr Butters own lab is developing.. We have a known mechanism of action for our compounds, and our initial experiments suggest that the drug works in a slightly different way. Both interfere with glycoprotein maturation of viral envelope proteins, ...
The flagship monthly journal of SPIE, |i|Optical Engineering|/i| (OE) publishes peer-reviewed papers reporting on research and development in all areas of optics, photonics, and imaging science and engineering.
Clone REA716 recognizes the human CD36L1 antigen, an integral membrane protein, also known as SR-BI. CD36L1 is widly expressed, e.g. on macrophages, dendritic cells, adrenocortical cells, adipocytes, and trophoblastic cells. In hepatocytes, CD36L1 acts as a receptor for hepatitis C. It has been reported, that CD36L1 is able to bind HCV envelope glycoprotein E2, participate in entry of HCV pseudotype particles, and modulate HCV infection. Furthermore, CD36L1 is a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, vitamin E, phosphatidylserine, and apoptotic cells. Additional information: Clone REA716 displays negligible binding to Fc receptors. - Canada
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Cellular entry of enveloped viruses is often dependent on attachment proteins expressed on the host cell surface. Viral envelope proteins bind these receptors, and, in an incompletely understood proce
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plasma proteins, enzymes, cytokines, ion channels, virus envelope proteins HIGH MOLECULAR MASS MULTIMER PROTEIN COMPLEXES, antobodies
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I have often referred to my disdain--hatred, really--of zines that arrive in the mail sans envelope, usually just taped or stapled shut with the address and stamp directly on the back cover. Oh how I despise this. I can see that you save some money; if you need 6x9 envelopes you save a whopping 7.2 cents an issue. So if your print run is 500 issues, youre saving like $36, which for some folks is a significant proportion of their rent or food budget that month--and lets face it, DIY publishers tend towards the poor side of existence, since we obviously have absolutely no concept of the value of our time ...
TreeHugger has a pretty soft spot in its modernist, hippie heart for flat-packing furniture; show us something that can be shipped in an (occasionally very large) envelope or slipped under a (really big) door and we start to salivate. Berlin-based
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A) Cells treated with different concentrations of SIN-1. 24 hpi, virus was . 4B, the luciferase activity in the cells infected with pseudotyped virus bearing S was In order to exclude any effect on the binding of the S protein to the ACE2 receptor. ...
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Plasmid pCerulean-VSVG from Dr. Jennifer Lippincott-Schwartzs lab contains the insert VSVG and is published in Nature. 1997 Sep 4. 389(6646):81-5. This plasmid is available through Addgene.
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TY - JOUR. T1 - Retraction. T2 - Dengue virus envelope protein domain I/II hinge determines long-lived serotype-specific dengue immunity (Proc Natl Acad Sci USA (2014) 111, (1939-1944) DOI: 10.1073/pnas.1317350111). AU - Messer, William. AU - De Alwis, Ruklanthi. AU - Yount, Boyd L.. AU - Royal, Scott R.. AU - Huynh, Jeremy P.. AU - Smith, Scott A.. AU - Crowe, James E.. AU - Doranz, Benjamin J.. AU - Kahle, Kristen M.. AU - Pfaff, Jennifer M.. AU - White, Laura J.. AU - Sariol, Carlos A.. AU - De Silva, Aravinda M.. AU - Baric, Ralph S.. PY - 2015/5/19. Y1 - 2015/5/19. UR - http://www.scopus.com/inward/record.url?scp=84929484676&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84929484676&partnerID=8YFLogxK. U2 - 10.1073/pnas.1506982112. DO - 10.1073/pnas.1506982112. M3 - Article. C2 - 25941397. AN - SCOPUS:84929484676. VL - 112. SP - E2738. JO - Proceedings of the National Academy of Sciences of the United States of America. JF - Proceedings of the National Academy of ...
Peptide microarray displaying 123 peptides derived from a peptide scan through Envelope glycoprotein E of West Nile virus (strain NY99-flamingo 382-99). Incubation with 20 samples...