The phylogenetic relationships of 50 reference strains, mostly marine bacteria which require Na+ for growth, were determined on the basis of 600 16S rRNA nucleotides by using reverse transcriptase sequencing. Strains belonging to 10 genera were included (four genera of the family Vibrionaceae, the g …
The cellular fatty acid compositions of 10 Vibrio species, two Aeromoas species, three Photobacterium species, Plesiomonas shigelloides, and Escherichia coli Were determined by using capillary gas-liquid chromatography (GLC). The major fatty acids in all Vibrionaceae species and E. coli were hexadecenoic, hexadecanoic, and octadecenoic acids. Qualitative and quantitative differences in hydroxy, branched, and cyclopropane fatty acids and in isomers of unsaturated 16- and 18-carbon acids were used to divide the 29 strains belonging to 17 species tested into 13 GLC groups. Of the 13 groups, 10 contained one species, 2 contained two species, and 1 contained three species. All of the Vibrionaceae cultures were differentiated from E. coli (GLC group I) because the concentration of hexadecenoic acid was greater than the concentration of hexadecanoic acid; in E. coli, this ratio was reversed. Aeromonas hydrophila (GLC group II) and Aeromonas salmonicida (GLC group III) were differentiated from the Vibrio and
Members of Vibrionaceae (Gammaproteobacteria: Vibrionales) have been known since 1854 (Pacini) and were shown to be distinct much before pulsed-field gel electrophoresis revealed the most distinct diagnostic morphological feature, the existence of two chromosomes [1]. The interest in these bacteria is not surprising given that several species are pathogenic to humans and marine organisms and others are bioluminescent symbionts of marine fishes and squids e.g.[2-7]. Some lesser-known species are psychrophiles (live in cold temperatures), piezophiles (live at high pressures), or halophiles (live at high NaCl concentration; [8]). The diversity of ecologies represented by members of Vibrionaceae has led to enthusiastic genome sequencing in the group, which has focused most heavily on pathogenic species (more than 31 strains of V. cholerae are available on GenBank as of 2012).. A phylogenetic hypothesis based on complete genomes was desired for Vibrionaceae. While the analysis presented in [9] for ...
Given the success of Volume I of this Research Topic, and the rapidly evolving subject area, we are pleased to announce the launch of Volume II: Molecular Adaptations of Vibrionaceae to Changing Environments.Vibrionaceae are Gram-negative bacteria that are present in nearly all aquatic ecosystems throughout the world. They can be found in the water column, in sediments or associated with aquatic organisms. Due to this variability of habitats, it is presumed that these bacteria have, or develop, various mechanisms to adapt to diverse, challenging and changing environments and sometimes be endemic to different ecological niches. Their capability to persist in different ecological habitats is reflected in numerous strategies to survive even in hostile environments. Mechanisms enabling horizontal gene transfer, antimicrobial resistance development, possession of different types of secretion systems, biofilm formation, modifications of cell wall structures and the ability to communicate via quorum sensing
Molecular surveys have revealed tremendous bacterial diversity in the worlds oceans; yet how do these diverse bacteria with the same essential nutrient requirements co-exist in the same environment? This study examines the role of aquatic microenvironments in generating bacterial diversity: closely related organisms may co-exist in the same environment without competing for resources by a combination of habitat, metabolic, and behavioral differentiation. This hypothesis has been approached from several angles: (i) Within the bacterial family Vibrionaceae is there evidence for microenvironmental specialization or functional differentiation? (ii) Is there small scale clustering of bacteria around phytoplankton in the coastal ocean? Microdiverse clusters (, 1% 16S rRNA gene divergence) of Vibrionaceae were found to be differentially distributed between zooplankton-enriched, particulate, and planktonic water column microenvironments. However microhabitat preferences may not correspond to metabolic ...
Vibrio cholerae is a bacterium causing the disease cholera. It is part of the genus Vibrio, in the family Vibrionaceae. Like all Proteobacteria, it is gram negative. V. cholerae can respire aerobically if oxygen is present and can switch to anaerobic respiration if oxygen is not present (fermentation).. ...
Presently, the long term objective of our laboratory is to determine the role of Aeromonas hydrophila virulence factors in the pathogenesis of disease in man. The virulence factors presently reported in the literature associated with A. hydrophila have not been defined clearly. A. hydrophila, a member of the family Vibrionaceae, is considered to be a significant human pathogen. This microorganism is responsible for a variety of diseases including acute bacterial diarrhea, septicemia, meningitis, endocarditis, corneal ulcers, peritonitis, and wound infections. These diseases have occurred in immunocompetent and well as immunocompromised individuals. A. hydrophila produce as well as possess a number of factors which contribute to the overall virulence of the organism. We have characterized two enterotoxins biochemically, biologically and antigenically. We have identified the genes for both enterotoxins and have cloned them. A complete nucleotide sequence analysis of the cytolytic enterotoxin gene ...
Vibrio: Vibrio, (genus Vibrio), any of a group of comma-shaped bacteria in the family Vibrionaceae. Vibrios are aquatic microorganisms, some species of which cause serious diseases in humans and other animals. Vibrios are microbiologically characterized as gram-negative, highly motile, facultative
Weekly cases of selected notifiable diseases ( ≥ 1,000 cases reported during the preceding year), and selected low frequency diseases, United States and U.S. territories, week ending December 29, 2018 (WEEK 52). TABLE 2r, Vibriosis (any species of the family Vibrionaceae, other than toxigenic Vibrio cholerae O1 or O139 ...
Weekly cases of selected notifiable diseases ( ≥ 1,000 cases reported during the preceding year), and selected low frequency diseases, United States and U.S. territories, week ending September 8, 2018 (WEEK 36). TABLE 2r, Vibriosis (any species of the family Vibrionaceae, other than toxigenic Vibrio cholerae O1 or O139 ...
Foodborne illnesses caused by bacterial microorganisms are common worldwide and constitute a serious public health concern. In particular, microorganisms belonging to the Enterobacteriaceae and Vibrionaceae families of Gram-negative bacteria, and to the Staphylococcus genus of Gram-positive bacteria are important causative agents of food poisoning and infection in the gastrointestinal tract of humans. Recently, variants of these bacteria have developed resistance to medically important chemotherapeutic agents. Multidrug resistant Escherichia coli, Salmonella enterica, Vibrio cholerae, Enterobacter spp., and Staphylococcus aureus are becoming increasingly recalcitrant to clinical treatment in human patients. Of the various bacterial resistance mechanisms against antimicrobial agents, multidrug efflux pumps comprise a major cause of multiple drug resistance. These multidrug efflux pump systems reside in the biological membrane of the bacteria and actively extrude antimicrobial agents from bacterial cells.
Author Summary Multi-drug resistant bacteria continue to emerge and there is a pressing need for the development of new antibiotics. Here, we carried out a cell-based high throughput screen to identify inhibitors of RctB, the initiator of replication of the second chromosome found in all the species of the Vibrionaceae. This family of bacteria includes several human pathogens, including Vibrio cholerae, the cause of cholera, as well as several species that damage economically important marine organisms. We identified a compound-designated vibrepin-that has potent cidal activity against V. cholerae and inhibited growth of all vibrio species tested. Vibrepin blocked RctB unwinding of the origin of replication of the second V. cholerae chromosome, apparently by promoting the formation of large non-functional RctB complexes. Vibrepin represents a new class of antibiotic that specifically targets a particular family of microorganisms (the Vibrionaceae). Such targeted agents will not engender resistance in
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio harveyi ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio harveyi ...
ID A0A084CNT0_9GAMM Unreviewed; 464 AA. AC A0A084CNT0; DT 29-OCT-2014, integrated into UniProtKB/TrEMBL. DT 29-OCT-2014, sequence version 1. DT 20-DEC-2017, entry version 26. DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256,HAMAP-Rule:MF_00377, ECO:0000256,RuleBase:RU000577, ECO:0000256,SAAS:SAAS00724181}; GN Name=dnaA {ECO:0000256,HAMAP-Rule:MF_00377, GN ECO:0000313,EMBL:KEY91459.1}; GN ORFNames=CF67_17030 {ECO:0000313,EMBL:KEY91459.1}; OS Candidatus Photodesmus blepharus. OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; OC Vibrionaceae; Candidatus Photodesmus. OX NCBI_TaxID=1179155 {ECO:0000313,EMBL:KEY91459.1, ECO:0000313,Proteomes:UP000053784}; RN [1] {ECO:0000313,EMBL:KEY91459.1, ECO:0000313,Proteomes:UP000053784} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Ppalp.1 {ECO:0000313,EMBL:KEY91459.1, RC ECO:0000313,Proteomes:UP000053784}; RA Hendry T.A., de Wet J.R., Dunlap P.V.; RT Selection and divergence in the genomes of co-occurring ...
ID C9P233_VIBME Unreviewed; 815 AA. AC C9P233; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 56. DE RecName: Full=Ribonuclease R {ECO:0000256,HAMAP-Rule:MF_01895}; DE Short=RNase R {ECO:0000256,HAMAP-Rule:MF_01895}; DE EC=3.1.13.1 {ECO:0000256,HAMAP-Rule:MF_01895}; GN Name=rnr {ECO:0000256,HAMAP-Rule:MF_01895}; GN ORFNames=VIB_000535 {ECO:0000313,EMBL:EEX38162.1}; OS Vibrio metschnikovii CIP 69.14. OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; OC Vibrionaceae; Vibrio. OX NCBI_TaxID=675813 {ECO:0000313,EMBL:EEX38162.1, ECO:0000313,Proteomes:UP000005604}; RN [1] {ECO:0000313,EMBL:EEX38162.1, ECO:0000313,Proteomes:UP000005604} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CIP 69.14 {ECO:0000313,EMBL:EEX38162.1, RC ECO:0000313,Proteomes:UP000005604}; RG Los Alamos National Laboratory (LANL); RG National Microbial Pathogen Data Resource (NMPDR); RA Munk A.C., Tapia R., Green L., Rogers Y., Detter ...
German officials said late Sunday that domestically grown bean sprouts may be the source of the Escherichia coli outbreak that has killed 22 people and sickened more than 2,100.
A father shows daughter the bean sprouts growing in the raised beds at the Grand Opening celebration of the Vallejo Peoples Garden on April 30, 2011
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The strain of bacteria that has caused lethal food poisoning in Germany is carried by bean sprouts and may have been circulating in people for 10 years ...
Plesiomonas shigelloides ATCC ® 51903D™ Designation: Genomic DNA from Plesiomonas shigelloides strain GNI 14 TypeStrain=False Application:
Plesiomonas shigelloides is the unique member of the Enterobacteriaceae family able to produce polar flagella when grow in liquid medium and lateral flagella when grown in solid or semisolid media. In this study on P. shigelloides 302-73 strain, we found two different gene clusters, one exclusively for the lateral flagella biosynthesis and the other one containing the biosynthetic polar flagella genes with additional putative glycosylation genes. P. shigelloides is the first Enterobacteriaceae were a complete lateral flagella cluster leading to a lateral flagella production is described. We also show that both flagella in P. shigelloides 302-73 strain are glycosylated by a derivative of legionaminic acid (Leg), which explains the presence of Leg pathway genes between the two polar flagella regions in their biosynthetic gene cluster. It is the first bacterium reported with O-glycosylated Leg in both polar and lateral flagella. The flagella O-glycosylation is essential for bacterial flagella formation,
Infections due to Aeromonas hydrophila and Plesiomonas shigelloides in immuno-compromised hosts have been well documented, but the role of these organisms in causing gastrointestinal disease in healthy persons is controversial. Despite difficulties in characterizing the exotoxins produced by Aeromonas species, there is accumulating evidence that these bacteria are capable of causing usually mild, self-limited diarrheal disease in previously healthy adults. Some pediatric patients may have protracted dysenteric symptoms. P.
There exists a rather colorful and winding history of interconnected pathways regarding the taxonomy and nomenclature associated with a group of enteric organisms now known as Plesiomonas shigelloides, the only recognized species in the genus. 16S rRNA sequencing indicates that members of the genera Serratia and Proteus are the closest phylogenetic neighbors to Plesiomonas. This chapter provides a list of the major disease categories associated with P. shigelloides infection and these categories include (i) septicemia; (ii) meningitis; (iii) intra-abdominal infections including peritonitis, abscesses, and hepatobiliary disease; (iv) ocular infections; and (v) a variety of miscellaneous illnesses involving infections of soft tissues and the urogenital tract. Epidemiologic, clinical, and laboratory data supporting the role of plesiomonads in bacterial gastroenteritis are discussed. The chapter also talks about complications that include cirrhosis, pernicious anemia, renal impairment, and diabetes mellitus
TY - JOUR. T1 - Crystal structure of α/β-galactoside α2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum. AU - Iwatani, Toru. AU - Okino, Nozomu. AU - Sakakura, Mai. AU - Kajiwara, Hitomi. AU - Takakura, Yoshimitsu. AU - Kimura, Makoto. AU - Ito, Makoto. AU - Yamamoto, Takeshi. AU - Kakuta, Yoshimitsu. PY - 2009/6/18. Y1 - 2009/6/18. N2 - α/β-Galactoside α2,3-sialyltransferase produced by Photobacterium phosphoreum JT-ISH-467 is a unique enzyme that catalyzes the transfer of N-acetylneuraminic acid residue from cytidine monophosphate N-acetylneuraminic acid to acceptor carbohydrate groups. The enzyme recognizes both mono- and di-saccharides as acceptor substrates, and can transfer Neu5Ac to both α-galactoside and β-galactoside, efficiently. To elucidate the structural basis for the broad acceptor substrate specificity, we determined the crystal structure of the α2,3-sialyltransferase in complex with CMP. The overall structure belongs to the ...
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In previous studies, the effects of environmental conditions on the growth rates of V. vulnificus were described for strains of unknown biotype (16, 35). We have determined that the growth rate of V. vulnificus in a nonselective, defined broth differed by biotype and that it was strongly affected by temperature and salinity and to a lesser extent by pH. These effects were observed whether the enumeration method was by culture (plate count) or qPCR analysis.. During our study, the fastest average generation time observed by culture methods was 15 min for biotype 1 (37°C, pH 7.0, 15 to 30‰ salinity). The generation time estimated by qPCR measurements under the same conditions was 24 min. The genome of V. vulnificus, as well as that of other members of the family Vibrionaceae, consists of two chromosomes. It has been suggested that the presence of two chromosomes could contribute to the high growth rate of V. vulnificus and other Vibrionaceae species (37). For example, V. parahaemolyticus and V. ...
Many clinical microbiology laboratories require that their identification systems perform at an accuracy of 90% or better, and many require a 95% accuracy. The Phoenix system accurately identifies isolates of the family Enterobacteriaceae only 89% of the time. Even with a weighted set of organisms, the accuracy is 89%.. In the only other published study of the Phoenix system that used conventional biochemicals for a reference method, Colodner et al. reported 90.2% accuracy when identifying 51 isolates of Vibrio vulnificus biotype 3 as Vibrio vulnificus (2). Because the present study did not include any biogroup 3 isolates, it is not known how the present results compare to those of Colodner and coworkers (2).. Several evaluations that used other commercial identification products as the reference method have been performed. Endimiani et al. tested 136 nonfermenting gram-negative bacilli and reported 95.6% agreement between the Phoenix 100 ID/AST system and the ATB/ID32GN system (bioMérieux, ...
Australia/Canberra: Forest Gate Organics recalls certain batch/lots of Mung Bean Sprouts due to suspected Salmonella contamination, a serious health hazard. ACCC: http://ht.ly/TRjc301jkCZ Direct link: http://www.recalls.gov.au/content/index.phtml/itemId/1088244 Additional information: The Australian Competition & Consumer Commission (ACCC) reports the following Bean Sprouts are subject to this recall: Mung Bean Sprouts sold in 200-gram plastic tubs with a best-before date…
For nearly half a century the Willamette River in Oregon experienced severe dissolved-oxygen problems related to large loads of organically rich waste waters from industries and municipalities. Since the mid-1950 s dissolved oxygen quality has gradually improved owing to low-flow augmentation, the achievement of basinwide secondary treatment, and the use of other waste-management practices. As a result, summer dissolved-oxygen levels have increased, salmon runs have returned, and the overall effort is widely regarded as a singular water-quality success. To document the improved dissolved-oxygen regimen, the U.S. Geological Survey conducted intensive studies of the Willamette during the summer low-flow seasons of 1973 and 1974. During each summer the mean daily dissolved-oxygen levels were found to be higher than 5 milligrams per liter throughout the river. Because of the basinwide secondary treatment, carbonaceous deoxygenation rates were low. In addition, almost half of the biochemical oxygen demand
Minimization of stochastic complexity (SC) was used as a method for classification of genotypic fingerprints. The method was applied to fluorescent amplified fragment length polymorphism (fAFLP) fingerprint patterns of 507 Vibrionaceae representatives. As the current BinClass implementation of the optimization algorithm for classification only works on binary vectors, the original fingerprints were discretized in a preliminary step using the sliding-window band-matching method, in order to maximally preserve the information content of the original band patterns. The novel classification generated using the BinClass software package was subjected to an in-depth comparison with a hierarchical classification of the same dataset, in order to acknowledge the applicability of the new classification method as a more objective algorithm for the classification of genotyping fingerprint patterns. Recent DNA-DNA hybridization and 16S rRNA gene sequence experiments proved that the classification based on ...
Coagulation factor X (FX) plays an important role in the immune response of mammals. In this study, the full length cDNA sequence of the ayu FX gene, 1817 bp in length excluding 3-polyA tail, was determined for the first time. The sequence contained an open reading frame, which encoded a protein of 453 amino acids with a molecular weight of 5.07 × 104. The predicted protein had motifs typical of animal FX, and its N-terminal 24 residues were the signal peptides. Sequence comparison showed that ayu FX shared 53% amino acid sequence identity with zebrafish FX. In healthy ayu, FX mRNA was expressed mainly in the liver and weakly in the brain and gill. After Listonella anguillarum ...
Bean sprouts are created by sprouting the seeds of certain beans, typically mung beans or soy. Youll find them in a lot of Eastern Asian dishes where they add a much-needed crunch to recipes like spring rolls, pad thai, fried rice and more. Theyre also great as salad mix-ins, sandwich toppers or stir-fry enhancers.. ...
1. Are bean sprouts safe for guinea pigs? 2. What about corn husks? 3. Arent all vegetables off limits to my pet guinea pig? 4. I heard people feed their pets beans and peas, but what do they mean by that exactly? 5. Why ...
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Lipid A is a major constituent of the lipopolysaccharides (or endotoxins), which are complex amphiphilic macromolecules anchored in the outer membrane of Gram-negative bacteria. The glycolipid lipid A is known to possess the minimal chemical structure for LPSs endotoxic activity, able to cause septic shock. Lipid A isolated from extremophiles is interesting, since very few cases of pathogenic bacteria have been found among these microorganisms. In some cases their lipid A has shown to have an antagonist activity, i.e., it is able to interact with the immune system of the host without triggering a proinflammatory response by blocking binding of substances that could elicit such a response. However, the relationship between the structure and the activity of these molecules is far from being completely clear. A deeper knowledge of the lipid A chemical structure can help the understanding of these mechanisms. In this manuscript, we present our work on the complete structural characterization of the lipid A
Its hard to believe, but there are similarities between bean sprouts and human cancer. The same mechanisms that result in bigger bean sprouts also cause cancer metastasis and tumor development.
In order to sprout the moong, first it has to be sorted to remove stones, washed, and then soaked in water for 6-8 hours. Wash the moong again and drain the Water. Subsequently, hang the soaked moong in a moist cloth for 10-12 hours (depending on the climate) or till you see the sprouts coming out of the muslin cloth. You may sprinkle water frequently if the cloth becomes dry. When the bean sprouts have developed short shoots, they are ready to eat ...
Pho ga, a Vietnamese chicken noodle soup, is topped with bean sprouts, lime juice, and Sriracha sauce for a refreshing and spicy meal.
The bacterium Vibrio natriegens grows even faster than E. coli, and it could cut in half the amount of time geneticists spend on routine experiments.
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Category:Pages edited by students of Tyrrell Conway at the University of Oklahoma]] {{Curated}} [[Image:OULOGOBIANCO.JPEG,thumb,230px,right,University of Oklahoma Study Abroad Microbiology in Arezzo, Italy[http://cas.ou.edu/study-abroad/]]] ==Etiology/Bacteriology== ===Taxonomy=== , Domain = [[Bacteria]] , Phylum = [[Proteobacteria]] , Class = [[Gammaproteobacteria]] , Order = [[Vibrionales]] , Family = [[Vibrionaceae]] , Genus = [[Vibrio]] , species = [[Vibrio parahaemolyticus]] [[Image: Vibrio-chol.jpg ,thumb,400px,right, ,i>Vibrio parahaemolyticus,/i>gram stain ,br /> From: http://www.google.it/imgres?imgurl&imgrefurl=http%3A%2F%2Fpathmicro.med.sc.edu%2Ffox%2Fenterobact.htm&h=0&w=0&sz=1&tbnid=sVqbkr3-2fJPxM&tbnh=190&tbnw=265&prev=%2Fsearch%3Fq%3Dvibrio%2Bparahaemolyticus%26tbm%3Disch%26tbo%3Du&zoom=1&q=vibrio%20parahaemolyticus&docid=YGvy0-93pwLbzM&hl=en&ei=aqLyUYrKB8aqO4uMgNgG&ved=0CAMQsCU]] ===Description=== ,i>Vibrio parahaemolyticus,/i> is a self-limiting, enterotoxic bacterium, typically ...
The effect of L. anguillarum and V. splendidus on the bacterial community associated with rotifers and seawater of rearing tanks was analysed by DGGE of PCR-amplified 16S rDNA fragments. The bacterial community of rotifers did not present a marked species dominance. The incorporation of L. anguillarum or V. splendidus did not reduce bacterial diversity and shifts could be explained by bacterial exchange between rotifers and seawater. Main bacterial groups were identified by sequencing the DNA extracted from Marine Agar (MA) bacterial isolates and DGGE excised bands. Only 2 DGGE bands corresponded to bacteria isolated from MA plates, suggesting that bacterial groups present in rotifers may not be easily cultivable. The bacterial community of rotifers was composed by Gram negative bacteria belonging to α-Proteobacteria (Ruegeria spp), γ-Proteobacteria (Alteromonas alvinellae, Marinobacter sp, an Oceanospirillaceae bacterium and Pseudoalteromonas sp), Cytophaga-Flexibacter-Bacteroides group ...
PDF - 29 pages] of healthy persons in which 6% reported eating bean sprouts in the week before they were interviewed. Most ill persons reported consuming bean sprouts at Asian-style food service establishments.. In this investigation, state and local officials identified five clusters of illnesses in three states: Massachusetts, Rhode Island, and Vermont. Among the ill persons who were part of these illness clusters, all reported consuming menu items that contained bean sprouts. Investigating clusters of illnesses can provide critical clues about the source of an outbreak. A cluster of illnesses is defined as more than one unrelated ill person (i.e., they do not know or live with each other) who report eating at the same restaurant location, attending a common event, or shopping at the same location of a grocery store before becoming ill. If several unrelated ill persons ate or shopped at the same location of a restaurant or store within several days of each other, it suggests that the ...
Two SEAFDEC/AQD scientists attended the 9th Symposium on Diseases in Asian Aquaculture (DAA9) from 24 to 28 November 2014 held at Ho Chi Minh City, Viet Nam.. During the conference, Dr. Rolando Pakingking Jr talked about his study on the Load and composition of the bacterial microbiota of tilapia (Oreochromis niloticus) cultured in earthen ponds in the Philippines. He reported that a total of 20 bacterial genera and 31 species were identified, majority of which were gram-negative bacteria constituting 84% of the overall bacterial isolates examined. Dominant bacteria identified in the gills and intestine of tilapia were Aeromonas hydrophila, Bacillus spp., Plesiomonas shigelloides, Shewanella putrefaciens, Pseudomonas fluorescens, Staphylococcus spp., and Vibrio cholerae. These bacteria were also dominant in the pond sediment and rearing water, except S. putrefaciens and V. cholerae which are not present in the water samples examined. According to Dr. Pakingking, this only indicates that ...
Enteric infections cause significant morbidity and mortality and have a significant financial cost worldwide. This chapter discusses the molecular techniques for the clinical identification of the bacteria, viruses, and parasites most commonly responsible for infections of the lower gastrointestinal tract. Numerous studies have reported on the development of PCR assays for the detection of enteric pathogens. Overall, multiplex, real-time PCR assay is favored because of its speed and elimination of postamplification carryover. The chapter summarizes the advancements achieved for the detection of enteric bacterial pathogens by molecular methods and discusses the remaining challenges. It also discusses the special case of hospital-acquired Clostridium difficile infection and the role of molecular methods in its diagnosis. Except for Plesiomonas shigelloides, which causes disease only in some patients, Salmonella, Shigella, and Yersinia enterocolitica are intrinsic enteric pathogens in humans. Importantly,
Gonz lez, Mario J et al. Aislamiento de Plesiomonas shigelloides y Aeromonas veronii biotipo sobria en heces de lobo marino com n sudamericano, Otaria flavescens (Shaw, 1800). Rev. biol. mar. oceanogr., Dic 2009, vol.44, no.3, p.763-765. ISSN 0718- ...
A case report of pneumonia and egg retention in a river terrapin was presented in this paper. A 20 year-old river terrapin (Batagur baska) was presented with complaint of coughing with blood discharge, wheezing breathing and inappetance. Plain radiograph showed both right and left lung lobes were consolidated due to pneumonia 90% and 70%, respectively. Tentative diagnosis of pneumonia due to opportunistic bacterial infection of Chromobacterium sp. and Plesiomonas shigelloides was made in this case based on bacterial isolation and response to antibiotic and anti-inflammatory drugs. Enrofloxacin and flunixin meglumine were administered from Day-1 to -5. Enrofloxacin was then changed to gentamicin since it showed resistance in those bacteria. No clinical changes were observed. Gentamicin was given at rate 2.5 mg/kg once a week intramuscularly and prednisolone 2 mg/kg once on Day-6 given intramuscularly, showed a response on Day-10 of hospitalisation with the evidence of reduction in lung ...
A couple of weeks ago I wrote a guest blog post on the China Law Blog describing the impact. The impact I suggested in that post was increased crackdowns on tainted products. Yesterday evidence of increased crackdowns came from a bean sprout bust.. The Legal Daily newspaper said in an online report that police in Shenyang had seized 40 tons of bean sprouts treated with the chemical compounds sodium nitrite and urea, as well as antibiotics and a plant hormone called 6-benzyladenine. Police arrested 12 people in connection with the seizure from six distribution centres and other locations around the city over three days last week. The newspaper estimated the seizure to amount to nearly one-third of all the bean sprouts in the citys markets. (Sodium nitrite hinders bacteria growth in food but can be toxic for humans and is carcinogenic. The plant hormone that was used is also banned as a food additive).. The newspaper explained Shenyangs problem with poison bean sprouts has been going on for ...
Fig. 4. Multilocus sequence typing analysis of bacteria presumed to be V. parahaemolyticus and other Vibrionaceae isolates. The phylogenetic tree was produced using the Jukes-Cantor model, with complete deletion of gaps and missing data, use of 1,000 bootstrap replicates, and the gamma 2.0 correction calculated using MEGA 3.1. Isolates are identified in a sample date code (e.g., the first sampling in May 2007 is 05071), followed by location (in a creek at high tide [H], ebb tide [E], low tide [L], or flood tide [F]; in a burrow in tall Spartina [TB], short Spartina [SB], and Juncus [JB]; and in pore water in tall Spartina [TP], short Spartina [SP], and Juncus [JP]) and isolate number. Asterisks indicate isolates presumed not to be V. parahaemolyticus. ...
In the current study, the construction of a toxR+ V. cholerae strain that expresses the ToxR-repressed porin OmpT in place of the ToxR-activated porin OmpU has allowed us to dissect the role these porins play in V. cholerae pathogenesis. Amazingly, merely substituting one outer membrane porin for another resulted in a number of attenuated pathogenic properties, including bile resistance, virulence factor expression, and intestinal colonization. ToxR-dependent regulation of outer membrane porins appears to have preceded the evolution of V. cholerae, because ToxR performs similar functions in other members of the Vibrionaceae (14, 17). However, these other bacteria do not have the additional regulatory factors TcpP and ToxT whose genes are located on the V. cholerae-specific pathogenicity island VPI (30). The recruitment of the ancestral ToxR protein into the virulence regulatory cascade involving recently acquired elements ties porin regulation to pathogenesis. We suggest that the contribution of ...
Vibrio alebo vibrión[1] je rod patriaci do čeľade Vibrionaceae. Najdôležitejšími druhmi sú Vibrio cholerae a Vibrio El Tor (dnes považované skôr za variant v rámci druhu V. cholerae), pôvodcovia cholery. Okrem nich existujú ďalšie patogénne druhy, ktoré môžu vyvolať ochorenia tráviaceho traktu i celkové ochorenia človeka. Typickým znakom rodu je rast tejto skupiny vo vode a citlivosť na koncentrácie solí v nej.. ...
SUMMARY: The metabolism of phospholipids in Vibrio costicola, a moderately halophilic bacterium, has been investigated in relation to sudden changes in salinity. Both the absqlute and relative rates of biosynthesis of phosphatidylglycerol and phosphatidylethanolamine depend on the salt concentration of the medium; a sudden rise in salt concentration ha? an instantaneous inhibitory effect on phospholipid biosynthesis, but this inhibition lessens as, the bacteria adapt to the higher salinity. There is no turnover of phospholipids during isotonic growth, nor when the salt concentration is suddenly altered. The alterations in biosynthetic rates of phosphatidylglycerol and phosphatidylethanolamine that occur after sudden changes in salt concentration are consistent with the known compositional changes. We conclude that the mechanisms of changes in phospholipid composition during adaptation to raised or lowered salt concentrations are not necessarily the same.
Vibrio costicola grown in the presence of different NaCl concentrations contains cell-associated Na+ and K+ ions whose sum is equal to or greater than the external Na+ concentration. In the presence of 0.5 M NaCl, virtually no in vitro protein is synthesized in extracts of cells grown in 1.0 M NaCl. However, we report here that active in vitro protein synthesis occurred in 0.6 M or higher concentrations of Na2SO4, sodium formate, sodium acetate, sodium aspartate, or sodium glutamate, whereas 0.6 M NaF, NaCl, or NaBr completely inhibited protein synthesis as measured by polyuridylic acid-directed incorporation of [14C]phenylalanine. Sodium glutamate, sodium aspartate, and betaine (0.3 M) counteracted the inhibitory action of 0.6 M NaCl. The cell-associated Cl- concentration was 0.22 mol/kg in cells grown in 1.0 M NaCl. Of this, the free intracellular Cl- concentration was only 0.02 mol/kg. Cells contained 0.11 mol of glutamate per kg and small concentrations of other amino acids. All of the ...
Nutrition Facts for Canned Mung Bean Sprouts - Get a bar chart of the top 10 nutrients, and click to see an expanded list of over 151 nutrients, including amino acids.
Egg Rolls Originalle Recipes containing ingredients bean sprouts, cabbage, carrots, egg whites, ginger root, green onions, hamburger, peanut butter, soy sauce,
Although many gut issues do not need lab testing, some people may be ordered to. People who have been traveling abroad are likely to be tested. According to the Centers for Disease Control and Prevention (CDC), 30 to 70 percent of those traveling outside the United States will get a form of gut infection. Other people who may need testing are the very young, the very old, and people with weakened immune systems. Another reason testing may occur if other symptoms like fever and dehydration are severe. If tests come back positive for bacteria such as Campylobacter, Salmonella, or Shigella, treatment may include antibiotics, but this is not always necessary. If the test is negative, it could be a less common bacterium such as Aeromonas, Plesiomonas, Yersinia enterocolitica, or a virus, and more tests may be ordered. If still negative for microbes like the ones above, a doctor may ask for another stool sample to investigate further. It is possible, but rare, to have more than one microbe present at ...
This article contributes several significant findings to the literature. The first is that we provide a sound mathematical framework that connects cognitive limits with macroscopic social structures using layered ER networks. Kudo and Dunbar [22] have suggested that social link formation is related to social group sizes but this is the first study to show how such discrete links between individuals structurally define the aggregate layering of society. We are also able to describe a specific cognitive process-that of forming multiple one-to-one social bonds-that is quantifiable from large datasets and then to show that typically humans are able to manage four or five such connections. Specifically, in extending from a smaller to a larger social group, this expansion can be identified with the addition of (on average) a single one-to-one link for every individual such that the majority of individuals form a unique core of connected individuals around which the group can then socially cohere. Some ...
In the process of beans become sprout, has outlined 90 percent olisakarida chain become simple carbohydrates, so that the compound is easily absorbed by the body, without producing gas. Because it contains a lot of fiber and water, sprouts help, draining impurities in the colon. It is become double power sprouts in the fight against cancer. By pushing dirt immediately left of the colon, so that no toxic substances in the dirt that can absorbed by the body. And this will prevent the buildup of toxic substances that can stimulate the blossoming of the seeds of cancer ...
Ed did have to finish the digging as I predicted. We shifted all the rubble between us (well dig up more next week but well have to get rid of it ourselves). No less than two other allotment holders gave us punnets of strawberries, and one told us to take any strawberry runners we wanted to overwinter, and another gave us a courgette plant. At the end of the day I nipped out to the supermarket and we had a barbecue on the site with allotment strawberries and cream for dessert. The Good Life? You bet ...
German officials announced on Friday that they believe bean sprouts are indeed the source of the deadly E. coli infections in that country, the worst such outbreak in recorded history.
When I was growing up, bean sprouts were the only Asian ingredient you could get and you could tell a good fried rice by the peas! Since moving to Byron Bay, its the flavours of Melbournes Chinatown and Victoria Street that I miss the most so sometimes you have to make it yourself. This is comfort food - which can be healthy in its own right if it makes you feel good and you only have it occasionally. I bought all the ingredients at a local Asian retailer and I cant vouch for the … [Read more...] about Special fried rice ...
Note: Read part 1 from Tam for the phở recipe and other tips. [Above photograph: Robyn Lee / Other photographs: Chichi Wang] Its impossible to keep phở a secret, Tam informed me. Such was the start of our phở get-together. She arrived at my apartment lugging a backpack crammed with gallons of phở broth, chunks of deboned oxtail, and little jars of fish sauce. Neatly sorted packets of noodles, bean sprouts, and mint completed the ensemble. If shed brought a portable stove, she couldve set up shop on my curb. Defatting the broth. Unlike Tam, I didnt grow up eating phở. My education of the broth began in Vietnamese noodle joints, where bowls of noodles are assembled and slapped onto...
Yes, I did share my dinner with Gracie this past weekend when I had Chinese takeout. Takeout and any kind of fast food is nothing I want to feed my dog (or myself) on a regular basis. Yet occasionally I enjoy it very much. Gracie can see and sense my enjoyment and its only natural for her to want to be part of the fun. And its only natural for me to want to share with her. Im sure Gracie thought last weekends Chinese takeout smelled irresistible. I knew if I didnt give her some shed watch me eat with sad, hopeful eyes while droplets of drool fell from her mouth. So I incorporated some Chinese takeout into Gracies dinner for the evening. To home-cooked chicken and brown rice I added some pork fried rice, some chopped broccoli, a few bean sprouts and two sesame shrimp with most of their battered coatings removed ...
Bean sprouts arent just for hippies. Theres method in that madness -- reducing the harmful effects of lectins, substances found in many foods. If youre experiencing GI distress, lectins may be the culprit. Read more about these ubiquitous substances, and how to control their effects.
If the Jews would just die without making a lot of noise, the Nice People could get on with the really important things in life, stuffing their faces with salmon and bean sprouts, watching the Rev. Billy Don Moyers pontificate on PBS, and making more Nice People.
Wild Rice is quite abundant in lakes of northwestern Ontario, but the southern Ontario variety has declined considerably and is ranked as S3. It is greatly affected by water levels. It seems to do best when the water levels are high in the spring, which suppresses some of the vegetation, but drops almost to the point of exposed mudflats by mid summer. If that happens, it can be the dominant vegetation. The large drainage ditch along Erieau Road just north of the McGeachy Pond area is quite full of Wild Rice. This particular photo shows only a single plant, but is easily identified by its height and form. Wild Rice is a favourite of wildlife. Ducks love it, and if you come across a dense stand in late August when Soras are migrating, toss a pebble into the stand during an evening visit and you may hear a chorus of peeps. I have done that in a very large stand of it and on occasion estimated there were at least 100 Soras peeping back at me ...