List of causes of Decreased LOC and Nerve symptoms and Sensation of intense heat and Skin symptoms and Heart rhythm symptoms and Vibrio infections, alternative diagnoses, rare causes, misdiagnoses, patient stories, and much more.
There are many ways via which climate change poses both direct and indirect consequences on the health of human beings. For instance, increased heat waves have led to the rise of heat-related illnesses. The emergence of intense floods and prolonged periods of rainfall have resulted to increased water-borne diseases such as amebiasis, Campylobacteriosis and Cholera. It has also resulted to amplified cases of Hepatitis A, Giardiasis, Viral Gastroenteritis, Vibrio infections, Cryptosporidiosis and
http://ir.library.oregonstate.edu/concern/administrative_report_or_publications/pg15bf53w Published January 1975. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog. ...
ABSTRAK Salah satu penyakit bacterial yang sering menimbulkan mortalitas tinggi di tempat tempat pembenihan udang windu (Penaeusmonodon Fab.) adalah penyakit kunang-kunang (hmtuescet vibriosis) yangdiakibatkan oleh bakteri ...
TY - JOUR. T1 - Characterization of a new plasmid-like prophage In a pandemic Vibrio parahaemolyticus O3:K6 strain. AU - Lan, Shih Feng. AU - Huang, Chung Ho. AU - Chang, Chuan Hsiung. AU - Liao, Wei Chao. AU - Lin, I. Hsuan. AU - Jian, Wan Neng. AU - Wu, Yueh Gin. AU - Chen, Shau Yan. AU - Wong, Hin Chung. PY - 2009/5/1. Y1 - 2009/5/1. N2 - Vibrio parahaemolyticus is a common food-borne pathogen that is normally associated with seafood. In 1996, a pandemic O3:K6 strain abruptly appeared and caused the first pandemic of this pathogen to spread throughout many Asian countries, America, Europe, and Africa. The role of temperate bacteriophages in the evolution of this pathogen is of great interest. In this work, a new temperate phage, VP882, from a pandemic O3:K6 strain of V. parahaemolyticus was purified and characterized after mitomycin C induction. VP882 was a Myoviridae bacteriophage with a polyhedral head and a long rigid tail with a sheath-like structure. It infected and lysed high ...
Vibrio parahaemolyticus O7兔多克隆抗体(ab79631)经Agg实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Editorial Note: V. vulnificus was first described as a cause of human illness in 1979 (2 ). Although there is no national surveillance for infections caused by this pathogen, regional surveillance in four states along the Gulf Coast indicates an annual incidence for V. vulnificus infections of at least 0.6 per 1 million persons and a case-fatality rate of 22% (3). V. vulnificus, a free-living bacterium, occurs naturally in the marine environment, rather than as a result of pollution by human or animal fecal waste. This organism is commonly found in estuarine waters of the Gulf of Mexico, where it may contaminate oysters and other shellfish. Legal harvesting of oysters is limited to areas free of fecal contamination; however, V. vulnificus is ubiquitous in warm ocean waters, and oysters harvested from approved sites may be contaminated. Therefore, regardless of the source of the oysters, the potential for infection exists whenever raw oysters are consumed. Ingestion of raw or undercooked ...
Category:Pages edited by students of Tyrrell Conway at the University of Oklahoma]] {{Curated}} [[Image:OULOGOBIANCO.JPEG,thumb,230px,right,University of Oklahoma Study Abroad Microbiology in Arezzo, Italy[http://cas.ou.edu/study-abroad/]]] ==Etiology/Bacteriology== ===Taxonomy=== , Domain = [[Bacteria]] , Phylum = [[Proteobacteria]] , Class = [[Gammaproteobacteria]] , Order = [[Vibrionales]] , Family = [[Vibrionaceae]] , Genus = [[Vibrio]] , species = [[Vibrio parahaemolyticus]] [[Image: Vibrio-chol.jpg ,thumb,400px,right, ,i>Vibrio parahaemolyticus,/i>gram stain ,br /> From: http://www.google.it/imgres?imgurl&imgrefurl=http%3A%2F%2Fpathmicro.med.sc.edu%2Ffox%2Fenterobact.htm&h=0&w=0&sz=1&tbnid=sVqbkr3-2fJPxM&tbnh=190&tbnw=265&prev=%2Fsearch%3Fq%3Dvibrio%2Bparahaemolyticus%26tbm%3Disch%26tbo%3Du&zoom=1&q=vibrio%20parahaemolyticus&docid=YGvy0-93pwLbzM&hl=en&ei=aqLyUYrKB8aqO4uMgNgG&ved=0CAMQsCU]] ===Description=== ,i>Vibrio parahaemolyticus,/i> is a self-limiting, enterotoxic bacterium, typically ...
A 3-year environmental and clinical Vibrio vulnificus survey using simple-sequence repeats typing shows that V. vulnificus biotype 3 constitutes ≈21% of the bacterium population in tested aquaculture ponds as opposed to ≈86% of clinical cases. Simple-sequence repeats proved to be a useful epidemiologic tool, providing information on the environmental source of the pathogen.
Vibrio vulnificus, a recently described strain of the halophilic Vibriospecies, was isolated from the blood of a 73-year-old man, who developed rapidly progressive wound infection and fatal...
Vibrio vulnificusis a gram-negative bacterium that can cause serious wound infections, septicemia, and diarrhea. It is the leading cause of shellfish-associated deaths in the United States. Infections due toV. vulnificusare most common in individuals
In developing countries, the fraction of treated wastewater effluents being discharged into watersheds have increased over the period of time, which have led to the deteriorations of the qualities of major rivers in developing nations. Consequently, high densities of disease causing bacteria in the watersheds are regularly reported including incidences of emerging Vibrio fluvialis. Vibrio fluvialis infection remains among those infectious diseases posing a potentially serious threat to public health. This paper addresses the epidemiology of this pathogen; pathogenesis of its disease; and its clinical manifestations in humans.
Abcam provides specific protocols for Anti-Vibrio parahaemolyticus O2 antibody (ab79616) : Vibro parahaemolyticus Agglutination Protocol
V. parahaemolyticus is autochthonous to the marine environment and causes seafood-borne gastroenteritis in humans. Generally, V. parahaemolyticus recovered from the environment and/or seafood is thought to be non-pathogenic and the relationship between environmental isolates and acute diarrhoeal disease is poorly understood. In this study, we explored the virulence potential of environmental V. parahaemolyticus isolated from water, plankton and assorted seafood samples collected from the Indian coast. Twenty-two V. parahaemolyticus isolates from seafood harboured virulence associated genes encoding the thermostable-direct haemolysin (TDH), TDH-related haemolysin (TRH), and Type 3 secretion systems (T3SS) and 95.5% of the toxigenic isolates had pandemic strain attributes (toxRS/new+). Nine serovars, with pandemic strain traits were newly identified and an O4:K36 tdh−trh+V. parahaemolyticus bearing pandemic marker gene was recognised for the first time. Results obtained by reverse transcription PCR
The CDC data on vibriosis includes all vibrio species except cholera, so its unclear how much of the increase in the past five years is due to infection by the flesh-eating bacteria that can cause death. [...] in a June 2012 letter to BP, Dr. Thomas Miller, the deputy director for medical affairs at the Alabama Department of Public Health stated, There is no epidemiological evidence to indicate increased rates of Vv (Vibriosis vulnificus) infections. In response to the EcoHealth article by Arias, Tao and Bullard, the U.S. Coast Guards on-scene coordinator for the oil spill management asked the National Oceanic and Atmospheric Administration to review the risk of Vibrio vulnificus infection due to tar ball exposure. In regard to the paper by Tao, Aris and Bullard, the NOAA report said, It is not surprising to find high levels of bacteria associated with these oil sources, although why V. vulnificus is present at such high levels is an intriguing question....The implications to public health also
Vibrio parahaemolyticus is a major foodborne pathogen, particularly in Asian countries. Increased occurrence of outbreaks of V. parahaemolyticus gastroenteritis in China indicates the need to evaluation of the prevalence of this pathogenic species. V. parahaemolyticus distribution in shellfish from the eastern coast of China has been reported previously. However, to date, the prevalence of V. parahaemolyticus in retail aquatic products in North China has not been determined. To investigate the prevalence of V. parahaemolyticus in aquatic products in North China, 260 aquatic product samples were obtained from retail markets in 6 provinces of North China from November to December in 2012 and July to August in 2013. V. parahaemolyticus was detected in 94 (36.2 %) of the samples by the most probable number method. The density of V. parahaemolyticus ranged from 1.50 to 1100 MPN/g. V. parahaemolyticus was detected at a rate of 50.0 % and 22.7 % in summer
The aim of this study was to detect and quantify Vibrio parahaemolyticus using flow cytometry (FCM) in combination with a polyclonal antibody developed in our laboratory. Experiments were carried out using V. parahaemolyticus cells in pure and mixed bacteria culture suspensions in either artificial or natural seawater. Using FCM, V. parahaemolyticus cells labelled with the polyclonal antibody and a secondary fluorescein isothiocyanate-conjugated antibody were detected and rapidly quantified at low cell densities (103 cells ml-1) in both the pure and mixed cultures. To determine the specificity of our antibody, its crossreactivity with other ATCC bacterial strains and some environmental Vibrio spp. and Gram-positive isolates was also assessed. Significant immunoreactivity levels above background were obtained for V. harvey 64, V. parahaemolyticus 704 and V. alginolyticus 1407, although the intensities were significantly less than for V. parahaemolyticus Conero. The experiments carried out in ...
Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, showed the appearance of the O4[ratio ]K68 serovar for the first time in March 1998 alongside the continued predominant incidence of the O3[ratio ]K6 serovar. Strains belonging to both these serovars have been reported to possess pandemic potential. The genomes of O3[ratio ]K6 and O4[ratio ]K68 strains and for comparison, non-O3[ratio ]K6 and non-O4[ratio ]K68 strains isolated from two different countries, India and Thailand, were examined by different molecular techniques to determine their relatedness. The O3[ratio ]K6 and O4[ratio ]K68 strains from Calcutta and Bangkok carried the tdh gene but not the trh gene. Characterization of representative strains of these two serovars by ribotyping and by arbitrarily primed-polymerase chain reaction (AP-PCR) showed that the isolates had identical ribotype and DNA fingerprint. Pulsed-field gel electrophoresis (PFGE) performed with the same set of ...
Vibrio parahaemolyticus, a Gram-negative motile bacterium that inhabits marine and estuarine environments throughout the world (7, 23, 36), is a major food-borne pathogen that causes life-threatening diseases in humans through the consumption of raw or undercooked seafood (5, 21, 34). V. parahaemolyticus infection can also occur through open wounds. The U.S. Centers for Disease Control and Prevention (CDC) reported cases of death resulting from wound infections by V. parahaemolyticus in 2005 (15). Given the harmful effects and prevalence of V. parahaemolyticus, it is very important to investigate its pathogenesis.. It is well known that V. parahaemolyticus contains many different kinds of toxins, such as thermostable direct hemolysin (TDH), TDH-related hemolysin (TRH), and some noncharacterized proteins. TDH is considered one of the major virulence factors of V. parahaemolyticus, and its function has been well characterized and discussed (25). TRH is another hemolysin of V. parahaemolyticus that ...
Author Summary Vibrio vulnificus causes disease both by infection of wounds from seawater and by consumption of contaminated foods, especially oysters. Wound infection results in necrotizing fasciitis and edema in extremities with mortality of ∼25% as the incidence of septicemia is low. Contaminated food consumption by contrast can lead to highly invasive infections that progress rapidly from an intestinal infection to primary septicemia. Case-fatality rates are ≥50%, with rates as high as 100% in individuals who receive no antibiotic therapy. The aim of this study is to elucidate virulence mechanisms of food-borne infection of the most highly virulent strains of V. vulnificus. We developed a novel intragastric infection model for a highly virulent clinical isolate from Korea in which we can observe the bacterial load in live mice and applied this to study of wild type and strains genetically altered to delete genes for two secreted cytotoxins. Using this model, we show that both the multifunctional
Vibrio parahaemolyticus is an emerging world-wide human pathogen that is associated with food-borne gastroenteritis when raw or undercooked seafood is consumed. Expression of virulence factors in this organism is modulated by the phenomenon known as quorum sensing, which permits differential gene regulation at low versus high cell density. The master regulator of quorum sensing in V. parahaemolyticus is OpaR. OpaR not only controls virulence factor gene expression, but also the colony and cellular morphology associated with growth on a surface and biofilm formation. Whole transcriptome Next Generation sequencing (RNA-Seq) was utilized to determine the OpaR regulon by comparing strains BB22OP (opaR+, LM5312) and BB22TR (∆opaR1, LM5674). This work, using the published V. parahaemolyticus BB22OP genome sequence, confirms and expands upon a previous microarray analysis for these two strains that used an Affymetrix GeneChip designed from the closely related V. parahaemolyticus RIMD2210633 genome ...
Vibrio parahaemolyticus is a food borne gastro-intestinal pathogen of major global importance. The organism induces severe inflammatory diarrhea in individuals who become infected due to the consumption of contaminated seafood. V. parahaemolyticus is known to exert pathogenic effects upon host cells by the translocation of effector proteins into the host cell, using a type three secretion injectisome, however little is known about the molecular interactions which allow colonisation and persistence of the organism in the intestinal epithelium. This study analysed the adherence and invasion of V. parahaemolyticus using the established intestinal cell line Caco-2. V. parahaemolyticus was found to adhere at high levels to Caco-2 cells. A small number of the adherent bacteria became internalised and were subsequently capable of intracellular proliferation. A genomic library based approach was undertaken in order to identify novel proteins conferring adhesive and invasive traits (adhesins/invasins) ...
As I explain in my book, Safe Food: The Politics of Food Safety, the FDA for more than a decade has been trying to prevent deaths caused by Vibrio vulnificus bacteria that contaminate raw oysters grown in the Gulf of Mexico. These "flesh-eating" bacteria proliferate in warm months and are especially deadly; they kill half of the thirty or so people who develop infections from it each year.. In 2001, the oyster industry trade association, the Interstate Shellfish Sanitation Conference (ISSC), promised the FDA that this industry would substantially reduce Vibrio infections in oysters within seven years through a program of voluntary self-regulation and education aimed at high-risk groups.. If this program failed to reduce the infection rate, the ISSC agreed that the FDA could require oysters to be treated after harvesting to kill pathogenic Vibrio.. Postharvest processing involves techniques such as quick freezing, frozen storage, high hydrostatic pressure, mild heat, or low dose gamma ...
In this study, we analyzed 260 aquatic product samples and detected V. parahaemolyticus contamination in 94 samples. Thus, the overall prevalence of V. parahaemolyticus in aquatic product samples was determined to be 36.2 %, which was in accordance with the results reported in a study from France [49] and in our previous study [18]. Notably, the prevalence of V. parahaemolyticus in summer (50.0 %) was higher than that in winter (22.7 %), and the levels of V. parahaemolyticus in the summer were significantly different from those in the winter. These results may be related to the differences in the average temperature of the two seasons. These observations were in agreement with the results of previous studies that showed a seasonal variation in the occurrence of this pathogen [50-52]. These results, which also confirm the conclusions of the WHO risk assessment for V. parahaemolyticus [53], can be useful for defining efficient monitoring programs in harvesting areas, based on temperature values ...
When encountering new environments or changes to their external milieu, bacteria use elaborate mechanisms to respond accordingly. Here we describe how Vibrio parahaemolyticus coordinates two such mechanisms - differentiation and chemotaxis. V. parahaemolyticus differentiates between two distinct cell types: short rod-shaped swimmer cells and highly elongated swarmer cells. We show that the intracellular organization of chemotactic signaling arrays changes according to the differentiation state. In swimmer cells chemotaxis arrays are strictly polarly localized, but in swarmer cells arrays form both at the cell poles and at irregular intervals along the entire cell length. Furthermore, the formation of lateral arrays increases with cell length of swarmer cells. Occurrence of lateral signaling arrays is not simply a consequence of the elongated state of swarmer cells, but is instead differentiation state-specific. Moreover, our data suggest that swarmer cells employ two distinct mechanisms for
Vibrio parahaemolyticus K30兔多克隆抗体(ab79495)经Agg实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Authorities believe that vibriosis infection and spectic shock is the cause. It may not have been completely the parasites fault, as doctors believe that years of alcohol abuse caused his body to become weakened.. "We present a case of Vibrio vulnificus septic shock and cellulitis in a patient with chronic liver disease that occurred after obtaining a leg tattoo with subsequent seawater exposure in the Gulf of Mexico," the summary from the British Medical Journal states. "Initial suspicion for V. vulnificus was high and he was started on empiric doxycycline and ceftriaxone at admission. Blood and wound cultures grew oxidase positive and comma-shaped Gram-negative rods ultimately confirmed to be V. vulnificus. Despite aggressive initial treatment, the patient developed septic shock and died. This case highlights the association of chronic liver disease and high mortality associated with infections of V. vulnificus. Health providers should remain vigilant for V. vulnificus infections in patients ...
Coloured scanning electron micrograph (SEM) of Vibrio parahaemolyticus, halophilic, facultative anaerobic, rod bacterium that causes a foodborne illness known as seafood poisoning. Usually transmitted through eating raw or undercooked seafood such as oysters. Less commonly, this organism can cause an infection in the skin when an open wound is exposed to warm seawater. Magnification: x4,000 when shortest axis printed at 25 millimetres. - Stock Image C032/1727
4E3R: Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin.
Vibrio parahaemolyticus is considered to be an emerging, yet understudied, human pathogen. The V. parahaemolyticus BB22OP genome was sequenced to allow for a comparative analysis between the genome of BB22OP and another previously sequenced, pathogenic strain of V. parahaemolyticus, RIMD2210633. V. parahaemolyticus BB22OP is interesting because it exhibits a spontaneous phenotypic switch in colony morphology due to the loss of a functional OpaR; this also influences virulence. OpaR is the major quorum-sensing regulator in V. parahaemolyticus homologous to LuxR from V. harveyi. When opaR is removed from the RIMD2210633 genome, the same phenotypic switch is not seen indicating a difference between the quorum-sensing systems in these two strains. Understanding the regulatory variation in these two strains has the potential to provide key insights into the control of pathogenesis in this organism. Initially, the BB22OP genome sequencing results aligned into 125 contigs. The genome has now been ...
Ph.D. - Texas A&M University. Research Interests:. My recent research has focused on microbial aspects of fresh and marine water quality and animal sources of fecal contamination affecting watersheds. This involves characterizing (antibiotic resistance and carbon source profiling) bacteria normally found in the intestinal tracts of animals such as E. coli and enterococci, using PCR and qPCR to detect host-specific genetic markers, and examining persistence of fecal bacteria in soils and sediments, as well as surface waters. We are presently conducting experiments to determine how well enterococci can survive in soils and later contaminate waters, under a range of environmental conditions. My students are also sampling a creek and an artificial lake for water quality and characterizing geese fecal bacteria. I have also been working with the estuarine pathogen Vibrio vulnificus, which causes severe wound infections, comparing environmental strains with clinical strains isolated from patients, for ...
BioAssay record AID 406643 submitted by ChEMBL: Antimicrobial activity against Vibrio anguillarum serotype O2b 04/09/367-5043 isolate containing gyrA position 83 Ser-Ile substitution mutation from Atlantic cod at 30 ug by disc diffusion assay.
Answers from specialists on vibriosis treatment options. First: Also named acitretin, is a retinoid used orally to treat psoriasis. Also, mometasone cream. Best to consult a dermatologist.
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Rabbit polyclonal Vibrio parahaemolyticus K33+K34+K36+K37+K38+K39+K40 antibody validated for Agg. Immunogen corresponding to tissue, cells or virus
4E3R: Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin.
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In a comparison of 2 formalin killed vaccines, SHAWOGEN using an isolate (AVC U-13220) of Vibrio anguillarum (serotype 2) isolated from cod, and a ...
This kit was designed for use by the first responding unit on scene, to set up an early command and triage structure while backup units are on their way
We present a case of a 40-year-old man with decompensated alcoholic liver cirrhosis presenting with atraumatic cellulitis of one extremity and severe sepsis that rapidly progressed to compartment syndrome despite broad-spectrum antibiotics. Local cultures following debridement revealed Vibrio vulnificus, and subsequent history revealed consumption of raw oysters 48 h before presentation. Our case points out the unique susceptibility of those with cirrhosis and elevated iron saturation to Vibrio septicaemia, as well as the rapidity and severity of the disease progression.
According to Centers For Disease Control and Prevention, Vibrio vulnificus is a kind of bacteria that may cause infections from eating raw or undercooked seafood. These infections can also happen through an open wound if the wound comes into contact with raw or undercooked seafood, its drippings or with saltwater.. "One species, Vibrio vulnificus, can even cause life-threatening wound infections. Many people with Vibrio vulnificus infection require intensive care or limb amputations, and about one in five people with this infection die, sometimes within a day or two of becoming ill," the CDC says.. "Some Vibrio vulnificus infections lead to necrotizing fasciitis, a severe infection in which the flesh around an open wound dies. Some media reports call this kind of infection "flesh-eating bacteria," even though necrotizing fasciitis can be caused by more than one type of bacteria." ...
During 1996-1997, a new Vibrio vulnificus biotype 3, which caused severe soft tissue infection after fishbone injury, emerged in Israel. We conducted a follow-up study from 1998 through 2005 to assess changing trends, outcomes, and molecular relatedness of the implicated strains. A total of 132 cases (71% confirmed and 29% suspected) of V. vulnificus biotype 3 infection were found. Most infections (95%) were related to percutaneous fish exposure, mainly tilapia (83%) or common carp (13%). Bacteremia, altered immune status, and history of ischemic heart disease were identified as independent risk factors for death, which reached a prevalence of 7.6%. Pulsed-field gel electrophoresis patterns of strains from 1998 through 2005 and from 1996 through 1997 showed a high degree of homogeneity and were distinct from those of V. vulnificus biotype 1. Infections caused by V. vulnificus biotype 3 continue affect the publics health in Israel.
The hfq gene is conserved in a wide variety of bacteria and Hfq is involved in many cellular functions such as stress responses and the regulation of gene expression. It has also been reported that Hfq is involved in bacterial pathogenicity. However, it is not clear whether Hfq regulates virulence in Vibrio parahaemolyticus. To evaluate this, we investigated the effect of Hfq on the expression of virulence-associated genes including thermostable direct hemolysin (TDH), which is considered to be an important virulence factor in V. parahaemolyticus, using an hfq deletion mutant. The production of TDH in the hfq deletion mutant was much higher than in the parental strain. Quantification of tdh promoter activity and mRNA demonstrated that transcription of the tdh gene was up-regulated in the mutant strain. The hfq-complemented strain had a normal (parental) amount of tdh expression. The transcriptional activity of tdhA was particularly increased in the mutant strain. These results indicate that Hfq is
A story map has been created to describe the different Vibrio Predictive Models produced by NCCOS. These predictive models help ensure oysters are harvested when they are safer to eat, assisting coastal managers and oyster growers in oyster harvest decision making nationwide.. Vibrio spp. are bacteria that occur naturally in our coastal waters. The majority of the cases annually are associated with Vibrio parahaemolyticus, usually resulting in self limiting gastroenteritis from the consumption of raw seafood. Vibrio vulnificus is another species of Vibrio bacteria that can cause human illness. Although cases are rare, Vibrio vulnificus infections stemming from water contact with open wounds or consumption can be serious and sometimes fatal.. The story map presents an assortment of predictive models and weather related tools to address issues surrounding safe harvest of shellfish around the nation. In partnership with the US Food and Drug Administration, state and academic partners, NOAA is ...
On August 7, this report was posted as an MMWR Dispatch on the MMWR website (http://www.cdc.gov/mmwr). During May 20--July 31, 2006, New York City, New York state, Oregon, and Washington health departments reported a total of 177 cases of Vibrio parahaemolyticus infection, of which 122 have been associated with 17 clusters. A cluster has been defined as a group of two or more ill persons who were linked to the same shellfish source (e.g., shared a meal at the same restaurant or obtained shellfish from the same seafood market). Certain clusters were associated with restaurants, certain clusters with seafood markets, and certain clusters with recreational harvesting. Three patients were hospitalized; no fatalities have been reported. No demographic (e.g., age, sex, or race) or medical history (e.g., predisposing conditions) information is yet available regarding affected persons. A confirmed case of V. parahaemolyticus infection is defined as an infection confirmed by isolation of the organism ...
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Here in the state of Florida, dozens of confirmed cases of the bacterial infection caused by Vibrio vulnificus occur annually, along with dozens more in the other Gulf states.. My guest, Infectious Disease specialist, a Forbes contributor, Judy Stone, MD said in a recent piece, "Infections from Vibrio vulnificus are a summer staple, and are increasing along with global warming.". Dr. Stone joined me to discuss Vibriosis caused by Vibrio vulnificus and the effects of warming seawaters on its presence in more parts of the world.. ...
TY - JOUR. T1 - An iron-regulated gene required for utilization of aerobactin as an exogenous siderophore in Vibrio parahaemolyticus. AU - Funahashi, Tatsuya. AU - Tanabe, Tomotaka. AU - Aso, Hiroaki. AU - Nakao, Hiroshi. AU - Fujii, Yoshio. AU - Okamoto, Keinosuke. AU - Narimatsu, Shizuo. AU - Yamamoto, Shigeo. PY - 2003/5/1. Y1 - 2003/5/1. N2 - A previous investigation using the Fur titration assay system showed that Vibrio parahaemolyticus possesses a gene encoding a protein homologous to IutA, the outer-membrane receptor for ferric aerobactin in Escherichia coli. In this study, a 5.6 kb DNA region from the V. parahaemolyticus WP1 genome was cloned and two entire genes, iutA and alcD homologues, were identified which are absent from Vibrio cholerae genomic sequences. The V. parahaemolyticus IutA and AlcD proteins share 43 % identity with the Escherichia coli IutA protein and 24 % identity with the Bordetella bronchiseptica AlcD protein of unknown function, respectively. Primer extension ...
TY - JOUR. T1 - Functional domains of a zinc metalloprotease from Vibrio vulnificus. AU - Miyoshi, Shin-ichi. AU - Wakae, H.. AU - Tomochika, K. I.. AU - Shinoda, S.. PY - 1997. Y1 - 1997. N2 - Vibrio vulnificus, an opportunistic human pathogen causing wound infection and septicemia, secretes a 45-kDa metalloprotease (V. vulnificus protease; VVP). A plasmid which carries the entire vvp gene subcloned into pBluescriptIIKS+ was transformed into Escherichia coil DH5α for overproduction of the protease. The 45-kDa recombinant protease (rVVP) was isolated from the periplasmic fraction of the transformant by ammonium sulfate precipitation followed by column chromatography on phenyl Sepharose. Biochemical characterization of the isolated rVVP showed that the recombinant protease was identical to that produced by V. vulnificus. When rVVP was incubated at 37°C, a 35-kDa fragment was generated through autoproteolytic removal of the C-terminal peptide. This 35-kDa fragment (rVVP-N) was found to have ...
Context In May and June 1998, reported Vibrio parahaemolyticus infections increased sharply in Texas. Objective To determine factors that contributed to the increase in V parahaemolyticus infections. Design, Setting, and ...