Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008 ...
Rab GTPases serve as molecular switches to regulate eukaryotic membrane trafficking pathways. The transport protein particle (TRAPP) complexes activate Rab GTPases by catalyzing GDP/GTP nucleotide exchange. In mammalian cells, there are two distinct TRAPP complexes, yet in budding yeast, four distinct TRAPP complexes have been reported. The apparent differences between the compositions of yeast and mammalian TRAPP complexes have prevented a clear understanding of the specific functions of TRAPP complexes in all cell types. In this study, we demonstrate that akin to mammalian cells, wild-type yeast possess only two TRAPP complexes, TRAPPII and TRAPPIII. We find that TRAPPIII plays a major role in regulating Rab activation and trafficking at the Golgi in addition to its established role in autophagy. These disparate pathways share a common regulatory GTPase Ypt1 (Rab1) that is activated by TRAPPIII. Our findings lead to a simple yet comprehensive model for TRAPPIII function in both normal and ...
Vesicle-associated membrane protein-associated protein A is a protein that in humans is encoded by the VAPA gene. Together with VAPB it forms the VAP protein family. The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. VAPA has been shown to interact with OSBP. This interaction is mediated by the FFAT motif in OSBP. GRCh38: Ensembl release 89: ENSG00000101558 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000024091 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Nishimura Y, Hayashi M, Inada H, Tanaka T (Feb 1999). "Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane ...
VAPB antibody for detecting human vesicle-associated membrane protein-associated protein B/C. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
Following the mutation screening of genes known to cause amyotrophic lateral sclerosis (ALS) in index cases from 107 familial ALS (FALS) kindred, a point mutation was identified in vesicle-associated membrane protein-associated protein B (VAPB), or VAMP-associated protein B, causing an amino acid change from threonine to isoleucine at codon 46 (T46I) in one FALS case but not in 257 controls. This is an important finding because it is only the second mutation identified in this gene that causes ALS. In order to investigate the pathogenic effects of this mutation, we have used a motor neuron cell line and tissue-specific expression of the mutant protein in Drosophila. We provide substantial evidence for the pathogenic effects of this mutation in abolishing the effect of wild type VAPB in the unfolded protein response, promoting ubiquitin aggregate formation, and activating neuronal cell death. We also report that expression of the mutant protein in the Drosophila motor system induces aggregate ...
VAPA antibody for detecting human vesicle-associated membrane protein-associated protein A. Validated on up to 12 cell lysates for western blotting. Try a trial size today.
In eukaryotic cells, membrane-bound vesicles carry cargo between intracellular compartments, to and from the cell surface, and to the extracellular environment. Many conserved families of proteins are required for properly localized vesicle fusion, including the multi-subunit tethering complexes and the SNARE complexes. These protein complexes work together to promote proper vesicle fusion in other trafficking pathways. Contrary to these other pathways, our lab previously suggested that the exocyst subunit Sec6, a component of the exocytosis-specific tethering complex, inhibited Sec9:Sso1 SNARE complex assembly due to interactions in vitro with the SNARE protein Sec9 (Sivaram et al., 2005). My goal for this project was to test the hypothesis that Sec6 inhibited SNARE complex assembly in vivo. I therefore chose to generate Sec6:Sec9 loss-of-binding mutants, and study their effect both in vitro and in vivo. I identified a patch of residues on Sec9 that, when mutated, are sufficient to disrupt the novel
Advanced multifunctional protein particles encapsulated enzymes and antibodies were developed for enzymatic bioassays and immunoassays with colorimetric and fluorescent channels. A colorimetric channel based on color-substrate precipitation was assigned for enzymatic bioassays for the measurement of hydrogen peroxide with the lowest detectable concentration of 10 μM. A fluorescent channel based on fluorescent labeled antibodies was assigned for immunoassays for the measurement of mouse immunoglobulin G (M IgG) with the lowest detectable concentration of 1.25 μg L−1. The protein microparticles were fabricated with a template-assisted self-assembly technique termed "Protein Activation Spontaneous Self-assemble" (PASS). The multifunctional protein particles prepared with the PASS method have the advantages of high loading of analytical biomolecules, integrated biological functions, porous structure, and more importantly, they are optically transparent and fluorescence inactive. These unique ...
Pulmonary delivery of proteins requires particles for delivery to be in the aerodynamic size range 1-5 μm for deep lung deposition. However, the traditional particle size reduction technique of jet-milling normally used for inhalation is not suitable for processing these protein particles because of their lability brought about by the weak physical interactions making up their higher order structures. Advanced techniques such as spray drying, spray freeze drying and the use of supercritical fluid technology have been developed to produce particles in the suitable size range and morphology for deep long deposition without altering the native conformation of these biomolecules. Judicious use of excipients and operating conditions are some of the factors needed for a successful particle design.. ...
GIRDers of actIN filament (Girdin) belongs to a family of proteins characterized by the presence of a coiled-coil domain. Girdin is an actin-binding protein that is activated by the serine/threonine kinase Akt. Girdin is a key modulator of the AKT-mTOR signaling pathway that controls the timing of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development, and synapse formation. It also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors (GPCRs). Elevated levels of Girdin expression are associated with cancer metastasis. Girdin is also known as coiled-coil domain containing 88A (CCDC88A), G alpha-interacting vesicle-associated protein, Hook-related protein 1, Akt-phosphorylation enhancer (APE), GIV, GRDN, HkRP1, and KIAA1212.. ...
GIRDers of actIN filament (Girdin) belongs to a family of proteins characterized by the presence of a coiled-coil domain. Girdin is an actin-binding protein that is activated by the serine/threonine kinase Akt. Girdin is a key modulator of the AKT-mTOR signaling pathway that controls the timing of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development, and synapse formation. It also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors (GPCRs). Elevated levels of Girdin expression are associated with cancer metastasis. Girdin is also known as coiled-coil domain containing 88A (CCDC88A), G alpha-interacting vesicle-associated protein, Hook-related protein 1, Akt-phosphorylation enhancer (APE), GIV, GRDN, HkRP1, and KIAA1212.. ...
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Although human blood is believed to be a sterile environment, recent studies suggest that pleomorphic bacteria exist in the blood of healthy humans. These studies have led to the development of
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
Complete information for TMED1 gene (Protein Coding), Transmembrane P24 Trafficking Protein 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
There is a large body of evidence linking small GTPases to the functional regulation of the exocyst complex (Mizuno-Yamasaki et al. 2012). At the same time, very few data exist on the contribution of phosphorylation-dephosphorylation cycles on exocyst regulation. In the present study, we set out to identify kinases and phosphatases that are functionally linked with the exocyst. For this a targeted RNAi screen was carried out in C. elegans animals mildly defective in the exocyst subunits Exo70 and Exo84. The screen identified seven kinases and seven phosphatase genes, down-regulation of which caused a variable degree of loss of viability when combined with exocyst mutations. For kin-20, the seventh kinase hit, a surprising partial rescue of the severely sick phenotype was observed in exoc-7 animals, and a full rescue was seen in exoc-8 mutants and in exoc-7; exoc-8 double mutants. kin-20 is the C. elegans homolog of the Drosophila circadian clock gene doubletime that is required to maintain ...
Rabbit polyclonal antibody raised against recombinant TMED8. Recombinant protein corresponding to amino acids of human TMED8. (PAB20042) - Products - Abnova
Kit Component:- KN317682G1, Tmed5 gRNA vector 1 in pCas-Guide vector- KN317682G2, Tmed5 gRNA vector 2 in pCas-Guide vector- KN317682D, donor vector…
Abstract: In order to understand the mechanism of molecular interactions at active sites of trafficking protein particle complex (TRAPPC) subunit 3-like protein (Accession number Q5T215) homology modeling and docking studies were taken up. We generated a three-dimensional (3D) model of target protein based on the Crystal structure of Human BET3 protein (PDB code 1SZ7) using modeller software. Under the process of homology modeling 25 models were generated, and the model having the lowest modeler objective function value was chosen for further assessment. The generated model was assessed and validated using PROCHECK software and found to be reliable.With the generated model, we carried out a flexible docking study using the FlexX docking tool available on the Sybyl Software in order to find better antagonist site for drug binding. We carried out a flexible docking with the Palmatic acid and Donepezil as ligands; these were found to bind at LEU87, LYS84 and THR90 residues on given generated ...
Fast delivery of TMED8 knockout Human Cell Lines for the study of gene function. Created by CRISPR/Cas9 genome editing. Includes matched wildtype control.
TMED10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 177 aa (32-185) having a molecular mass of 20.0kDa.
Complete information for VPS35 gene (Protein Coding), VPS35, Retromer Complex Component, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
YIF1A - YIF1A (untagged)-Human Yip1 interacting factor homolog A (S. cerevisiae) (YIF1A) available for purchase from OriGene - Your Gene Company.
EXOC7 overexpression lysate, 0.1 mg. Transient overexpression lysate of exocyst complex component 7 (EXOC7), transcript variant 5
Kit Component:- KN317680G1, Tmed3 gRNA vector 1 in pCas-Guide vector- KN317680G2, Tmed3 gRNA vector 2 in pCas-Guide vector- KN317680D, donor vector…
Pasta de dinti BIO pentru copii cu capsuni si zmeura, Basis Sensitiv - LAVERA, 75ml Descriere Inlatura placa bacteriana si ofera protectie impotriva cariilor. Nu contine tenside sau fluor. Dintii sanatosi...
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Acestea sunt insa recomandate doar pentru persoanele care prezinta anumite proteine in celulele canceroase, prin urmare este necesata o biopsie prealabila.
کنترل زمان گل‌دهی یکی از مهم‌ترین اجزای اثر متقابل بین گیاهان و محیط رشد آن‌ها می‌باشد که نه تنها برای میزان محصول تولیدی بلکه برای کیفیت دانه برنج نیز عامل مهمی به‌-حساب می‌آید. در این تحقیق مطالعات فنوتیپی و مولکولی بر روی 45 رقم برنج محلی و اصلاح شده انجام شد. ابتدا چندشکلی ژن‌های Ehd1 و Ehd3 در بین ارقام و سپس ارتباط این دو ژن با زمان خوشه‌دهی مورد بررسی قرار گرفت. نتایج مطالعات فنوتیپی حاکی از وجود تنوع بیشتر در ارقام محلی نسبت به ارقام اصلاح شده بود. ارقام محلی به‌طور متوسط 8 روز زودرس‌تر از ارقام اصلاح شده بودند و تفاوت زمان خوشه‌دهی آن‌ها معنی‌دار
Assembly of primary cilia relies on vesicular trafficking towards the cilium base and intraflagellar transport (IFT) between the base and distal tip of the cilium. Recent studies have identified several key regulators of these processes, including Rab GTPases such as Rab8 and Rab11, the Rab8 guanine nucleotide exchange factor Rabin8, and the transport protein particle (TRAPP) components TRAPPC3, -C9, and -C10, which physically interact with each other and function together with Bardet Biedl syndrome (BBS) proteins in ciliary membrane biogenesis. However, despite recent advances, the exact molecular mechanisms by which these proteins interact and target to the basal body to promote ciliogenesis are not fully understood. We surveyed the human proteome for novel ASPM, SPD-2, Hydin (ASH) domain-containing proteins. We identified the TRAPP complex subunits TRAPPC8, -9, -10, -11, and -13 as novel ASH domain-containing proteins. In addition to a C-terminal ASH domain region, we predict that the N-terminus of
Exocyst complex component 2 is a protein that in humans is encoded by the EXOC2 gene. The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. GRCh38: Ensembl release 89: ENSG00000112685 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000021357 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Murthy M, Garza D, Scheller RH, Schwarz TL (Feb 2003). "Mutations in the exocyst component Sec5 disrupt neuronal membrane traffic, ...
The exocyst is a protein complex that has been found to be essential for exocytosis underlying neurite outgrowth (Hsu et al., 2004). Several models have been proposed to explain how the exocyst complex promotes exocytosis, including modulating cytoskeletal activity and tethering vesicles to the plasma membrane. Targeting of the exocyst complex to spatially defined domains, such as growth cones, is expected to be essential for a focused function of the exocyst complex. In this regard, exocyst subunits have been found to associate with various scaffold proteins such as PSD95 and SAP102 that target plasma membrane proteins to specific plasma membrane subdomains (Riefler et al., 2003; Sans et al., 2003) or with plasma membrane receptors, such as the glycine receptor GLYT1 (Cubelos et al., 2005).. In this manuscript, we identify NCAM as a novel binding partner of the exocyst complex. Several studies have shown that NCAM plays an important role in neural development by regulating neurite outgrowth. In ...
The results of yeast and mammalian two-hybrid assays previously indicated complex formation between prenylated Rab acceptor 1 (PRA1) and the cytoplasmic domain of gp41 (gp41CD) for both the human and simian immunodeficiency viruses [ Evans, D. T., Tilman, K. C. & Desrosiers, R. C. (2002). J Virol 76, 327-337 ]. The assembly and release of infectious virus particles was studied under conditions of PRA1 overexpression in a transient transfection assay or suppression by RNA interference. Although a clear pattern of co-localization of PRA1 and gp41 was observed, no changes in virion release, infectivity or envelope content were observed as a result of either PRA1 suppression or overexpression. These data show that PRA1 co-localizes with gp41 inside cells and they are consistent with a direct or indirect interaction between these proteins. However, variation in the levels of PRA1 expression did not influence virion production, infectivity or envelope incorporation under the conditions of these assays.
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB).
Prenylated Rab acceptor 1 domain family, member 2 (PRAF2) is a novel 19-kDa protein with four transmembrane-spanning domains that belongs to the PRAF protein family. Neuroblastoma (NB) is the most common malignant extracranial solid tumor of childhood that originates in primitive cells of the developing sympathetic nervous system. We investigated the correlation of PRAF2 mRNA expression to NB clinical and genetic parameters using Affymetrix expression analysis of a series of 88 NB tumors and examined the functional role of PRAF2 in an NB cell line using RNA interference. We found that high PRAF2 expression is significantly correlated to several unfavorable NB characteristics: MYCN amplification, high age at diagnosis, poor outcome and high INSS stage. The shRNA-mediated PRAF2 downregulation in the SK-N-SH NB cell line resulted in decreased cellular proliferation, migration and matrix-attachment. These findings were confirmed in NB patient tumor samples, where high PRAF2 expression is ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and it encodes a protein similar to the yeast class C Vps33 protein. The mammalian class C VPS proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq, Jul 2008 ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008 ...
On page 1029, Supek et al. illustrate how a peripheral membrane protein organizes a coat protein complex involved in secretory vesicle formation.. The protein in question, yeast Sec16p, is an ER resident required in vivo for COPII-dependent vesicle budding. In vitro, Sec16p is not necessary for budding from liposomes reconstituted with pure cytosolic COPII proteins. However, this in vitro reaction depends on a nonhydrolyzable form of GTP, probably because the COPII coat falls apart when Sar1p (the initiator of coat assembly) hydrolyzes GTP. Until now, the function of Sec16p in liposome budding could not be tested, because the protein was difficult to purify. Supek et al. report conditions that stabilize Sec16p and have purified enough protein for in vitro studies. Microsomal membranes stripped of endogenous Sec16p were stimulated in vesicle budding by the purified. protein, but only in the presence of hydrolyzable GTP. Thus, the in vivo function of Sec16p may be either to slow GTP hydrolysis ...
antibody-antibodies.com is the marketplace for research antibodies. Find the right antibody for your research needs. Deregulation of Rab and Rab Effector Genes in Bladder Cancer
TNF receptor-associated protein 1 (TRAP1), the main mitochondrial member of the heat shock protein (HSP) 90 family, is induced in most tumor types and is involved in the regulation of proteostasis in the mitochondria of tumor cells through the control of folding and stability of selective proteins, such as Cyclophilin D and Sorcin. Notably, we have recently demonstrated that TRAP1 also interacts with the regulatory protein particle TBP7 in the endoplasmic reticulum (ER), where it is involved in a further extra-mitochondrial quality control of nuclear-encoded mitochondrial proteins through the regulation of their ubiquitination/degradation. Here we show that TRAP1 is involved in the translational control of cancer cells through an attenuation of global protein synthesis, as evidenced by an inverse correlation between TRAP1 expression and ubiquitination/degradation of nascent stress-protective client proteins. This study demonstrates for the first time that TRAP1 is associated with ribosomes and ...
Protein synthesis. Computer artwork of protein being synthesized by a ribosome. Ribosomes are protein particles that are found in cell cytoplasm. Each ribosome has a large and a small subunit. Messenger ribonucleic acid (mRNA, purple) passes between the two subunits and provides the instructions for the assembly of a protein (polypeptide) chain (yellow) from amino acids. The mRNA originates in the cells nucleus, and is a copy of the information coded in DNA (deoxyribonucleic acid). The process of protein synthesis from mRNA in ribosomes is known as translation. Different sequences of mRNA produce different proteins. - Stock Image G110/0840
Antibodies developed by researchers at Rensselaer Polytechnic Institute are unusually effective at preventing the formation of toxic protein particles linked to Alzheimers disease and Parkinsons disease, as well as Type ...
EEA1 antibody [C3], C-term (early endosome antigen 1) for ICC/IF, IHC-P, WB. Anti-EEA1 pAb (GTX109638) is tested in Human, Mouse, Rat, Hamster samples. 100% Ab-Assurance.
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InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
EXOC2 antibody (exocyst complex component 2) for ELISA, IHC-P. Anti-EXOC2 pAb (GTX85724) is tested in Human samples. 100% Ab-Assurance.
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