TY - JOUR. T1 - Safety studies on intravenous administration of oncolytic recombinant vesicular stomatitis virus in purpose-bred beagle dogs. AU - Leblanc, Amy K.. AU - Naik, Shruthi. AU - Galyon, Gina D.. AU - Jenks, Nathan. AU - Steele, Mike. AU - Peng, Kah Whye. AU - Federspiel, Mark J.. AU - Donnell, Robert. AU - Russell, Stephen J.. PY - 2013/12/1. Y1 - 2013/12/1. N2 - VSV-IFNβ-NIS is a novel recombinant oncolytic vesicular stomatitis virus (VSV) with documented efficacy and safety in preclinical murine models of cancer. To facilitate clinical translation of this promising oncolytic therapy in patients with disseminated cancer, we are utilizing a comparative oncology approach to gather data describing the safety and efficacy of systemic VSV-IFNβ-NIS administration in dogs with naturally occurring cancer. In support of this, we executed a dose-escalation study in purpose-bred dogs to determine the maximum tolerated dose (MTD) of systemic VSV-hIFNβ-NIS, characterize the adverse event ...
Viruses need us. In order to multiply, viruses have to invade a host cell and copy their genetic information. To do so, viruses encode their own replication machinery or components that subvert the host replication machinery to their advantage.. Ebola virus and rabies virus, two of the most lethal pathogens known to humans, belong to an order of RNA viruses that share a common strategy for copying their genomes inside their hosts. Other relatives include Marburg virus, measles, mumps, respiratory syncytial virus and vesicular stomatitis virus (VSV). Scientists study VSV, which causes acute disease in livestock but typically does not lead to illness in people, as a model for viruses that are harmful to humans.. Now a team from Harvard Medical School, using electron cryomicroscopy (imaging frozen specimens to reduce damage from electron radiation), has for the first time revealed the structure of a VSV protein at the atomic level. Called polymerase protein L, it is required for viral replication ...
TY - JOUR. T1 - Vesicular stomatitis virus glycoprotein contains a dominant cytoplasmic basolateral sorting signal critically dependent upon a tyrosine. AU - Thomas, DNette C.. AU - Brewer, Colleen B.. AU - Roth, Michael G.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - To investigate the contribution of the cytoplasmic domain of the vesicular stomatitis virus G glycoprotein to its basolateral expression in polarized epithelial cells, chimeric proteins containing the external and transmembrane domains of an apically targeted protein, the influenza virus hemagglutinin (HA), and either the G cytoplasmic domain or an unrelated cytoplasmic sequence, were introduced into Madin-Darby canine kidney (MDCK) cells. Addition of the cytoplasmic tail of G to a truncated HA resulted in delivery of greater than 95% of the chimeric protein to the basolateral cell surface, indicating that the G cytoplasmic domain contains a dominant basolateral sorting signal. A similar chimera, containing the cytoplasmic tail of herpes ...
The cytopathogenicity of vesicular stomatitis virus (VSV) has been attributed mainly to the host shut-off activity of the viral matrix (M) protein, which inhibits both nuclear transcription and nucleocytoplasmic RNA transport, thereby effectively suppressing the synthesis of type I interferon (IFN). The M protein from persistently VSV-infected cells was shown to harbour characteristic amino acid substitutions (M51R, V221F and S226R) implicated in IFN induction. This study demonstrates that infection of human fibroblasts with recombinant VSV containing the M51R substitution resulted in IFN induction, whereas neither the V221F nor the S226R substitution effected an IFN-inducing phenotype. Only when V221F was combined with S226R were the host shut-off activity of the M protein abolished and IFN induced, independently of M51R. The M33A substitution, previously implicated in VSV cytotoxicity, did not affect host shut-off activity. M-mutant VSV containing all four amino acid substitutions retained cytotoxic
A stable cell line expressing a complementary DNA clone encoding the vesicular stomatitis virus glycoprotein fused and formed polykaryons at pH 5.5. The formation of polykaryons was dependent on the presence of glycoprotein anchored at the cell surface and could be prevented by incubation of cells with a monoclonal antibody to the glycoprotein. Fusion occurred at a pH 0.5 unit lower than that observed for cells infected with vesicular stomatitis virus. ...
The particle-bound RNA polymerase activity of vesicular stomatitis virus (VSV) can be demonstrated in vivo. Linear synthesis of viral RNA persists for 5 to 6 hours at 34°C in infected monolayers of chick embryo cells treated with cycloheximide and actinomycin D to block synthesis of protein and cell-specific RNA. At least 55 percent of the RNA made under these conditions is complementary to virion RNA. RNA synthesis mediated by VSV polymerase activity is inhibited in cells first treated with chick-derived interferon or polyriboinosinate• polyribocytidylate, but not by mouse interferon. The RNA product of VSV polymerase activity is present throughout the cytoplasm, and its synthesis is inhibited by the interferon system, as judged by autoradiographs that show the physical distribution, in cells, of RNA produced by virion polymerase in the absence of translation-a demonstration of the transcription product of the viral genome. ...
Our findings demonstrate an important role for PERK in VSV infection. We show that PERK-mediated eIF2α phosphorylation is induced in VSV-infected cells and that this is accompanied by an inhibition of virus replication and apoptosis. PERK also plays an essential role in UPR (31) and, as such, its activation in VSV infection initially indicated an ability of the virus to elicit a UPR. This was consistent with the notion that viruses that use the ER as an integral part of their replication strategy are likely able to induce an ER stress response (1). In fact, previous studies showed that the VSV glycoprotein (VSV-G) oligomerizes in the ER prior to its transport to the cell surface (41). Misfolded and unassembled VSV-G is retained in the ER (8), whereas the interactions of the viral protein with two chaperones, BiP and calnexin, are essential for efficient folding and for retention of partially folded G protein forms in the ER (12). Thus, an overload of VSV-G in the ER during virus replication ...
The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely replaced by the G glycoprotein of vesicular stomatitis virus. Such vectors can be concentrated by ultracentrifugation to titers , 10(9) colony-forming units/ml and can infect cells, such as hamster and fish cell lines, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein. The ability to concentrate vesicular stomatitis virus G glycoprotein pseudotyped vectors will facilitate gene therapy model studies and other gene transfer experiments that require direct delivery of vectors in vivo. The availability of these pseudotyped vectors will also facilitate genetic studies in nonmammalian species, including the important zebrafish developmental system, through the efficient ...
In this study, we show that posttranslational folding of Vesicular Stomatitis virus G protein subunits can involve noncovalent, multimeric complexes as transient intermediates. The complexes are heterogeneous in size (4-21S20,W), contain several G glycopolypeptides, and are associated with BiP/GRP78. The newly synthesized, partially intrachain disulfide-bonded G proteins enter these complexes immediately after chain termination, and are released 1-4 min later as fully oxidized, trimerization-competent monomers. These monomers are properly folded, judging by their binding of conformation-specific mAbs. When the G protein is translated in the presence of DTT, it remains reduced, largely unfolded and aggregated in the ER, but it can fold successfully when the DTT is removed. In this case, contrary to normal folding, the aggregates become transiently disulfide cross-linked. We also demonstrated that the fidelity of the folding process is dependent on metabolic energy. Finally, we established that ...
In a previous study we demonstrated that vesicular stomatitis virus (VSV) can be used as a vector to express a soluble protein in mammalian cells. Here we have generated VSV recombinants that express four different membrane proteins: the cellular CD4 protein, a CD4-G hybrid protein containing the ectodomain of CD4 and the transmembrane and cytoplasmic tail of the VSV glycoprotein (G), the measles virus hemagglutinin, or the measles virus fusion protein. The proteins were expressed at levels ranging from 23-62% that of VSV G protein and all were transported to the cell surface. In addition we found that all four proteins were incorporated into the membrane envelope of VSV along with the VSV G protein. The levels of incorporation of these proteins varied from 6-31% of that observed for VSV G. These results suggest that many different membrane proteins may be co-incorporated quite efficiently with VSV G protein into budding VSV virus particles and that specific signals are not required for this ...
TY - JOUR. T1 - Delayed appearance of pseudotypes between vesicular stomatitis virus and influenza virus during mixed infection of MDCK cells. AU - Roth, M. G.. AU - Compans, R. W.. PY - 1981/12/1. Y1 - 1981/12/1. N2 - In intact Madin-Darby canine kidney (MDCK) cell monolayers, vesicular stomatitis virus (VSV) matures only at basolateral membranes beneath tight junctions, whereas influenza virus buds from apical cell surfaces. Early in the growth cycle, the viral glycoproteins are restricted to the membrane domain from which each virus buds. We report here that phenotypic mixing and formation of VSV pseudotypes occurred when influenza virus-infected MDCK cells were superinfected with VSV. Up to 75% of the infectious VSV particles from such experiments were neutralized by antiserum specific for influenza virus, and a smaller proportion (up to 3%) were resistant to neutralization with antiserum specific for VSV. The latter particles, which were neutralized by antiserum to influenza A/WSN virus, ...
Innate immune activation is essential to mount an effective antiviral response and to prime adaptive immunity. Although a crucial role of CD169+ cells during vesicular stomatitis virus (VSV) infections is increasingly recognized, factors regulating CD169+ cells during viral infections remain unclear. Here we show that tumor necrosis factor is produced by CD11b+ Ly6C+Ly6G+ cells following infection with VSV. The absence of TNF or TNF receptor 1 (TNFR1) resulted in reduced numbers of CD169+ cells and in reduced IFN-I production during VSV infection, with a severe disease outcome. Specifically, TNF triggered RelA translocation into the nucleus of CD169+ cells; this translocation was inhibited when paracaspase MALT-1 was absent. Consequently, MALT1 deficiency resulted in reduced VSV replication, defective innate immune activation, and severe disease development. These findings indicate that TNF mediates the maintenance of CD169+ cells and innate and adaptive immune activation during VSV ...
Summary Multiply cloned variants of vesicular stomatitis virus (VSV) were found to generate/amplify defective interfering (DI) particles at a rate greatly exceeding the rates normally observed for wild-type VSV (or for other mutants of VSV). A single undiluted passage of the first clonal pool of this variant virus produced concentrated visible bands of DI particles on sucrose gradients whereas wild-type and other mutant strains of VSV required from three to six or more serial undiluted passages. Since DI particle amplication by wild-type VSV at each undiluted passage can exceed 10000-fold enrichment, these variant virus clones were generating/amplifying DI particles many millions of times more rapidly than were wild-type and other mutant strains of VSV. This rate of generation/amplification is so high that it was not feasible to obtain accurate estimates of the rates of generation (or amplification) of these DI particles.
Vaccines based on live viruses are attractive because they are immunogenic, cost-effective, and can be delivered by multiple routes. However, live virus vaccines also cause reactogenic side effects such as fever, myalgia, and injection site pain that have reduced their acceptance in the clinic. Several recent studies have linked vaccine-induced reactogenic side effects to production of the pro-inflammatory cytokine interleukin-1β (IL-1β) in humans. Our objective was therefore to determine whether IL-1β contributed to pathology after immunization with recombinant vesicular stomatitis virus (rVSV) vaccine vectors, and if so, to identify strategies by which IL-1β mediated pathology might be reduced without compromising immunogenicity. We found that an rVSV vaccine induced local and systemic production of IL-1β in vivo, and that accumulation of IL-1β correlated with acute pathology after rVSV immunization. rVSV-induced pathology was reduced in mice deficient in the IL-1 receptor Type I, but the IL-1R
The Brighton Collaboration Viral Vector Vaccines Safety Working Group (V3SWG) was formed to evaluate the safety of live, recombinant viral vaccines incorporating genes from heterologous viral and other microbial pathogens in their genome (so-called "chimeric virus vaccines"). Many such viral vector vaccines are now at various stages of clinical evaluation. Here, we introduce an attenuated form of recombinant vesicular stomatitis virus (rVSV) as a potential chimeric virus vaccine for HIV-1, with implications for use as a vaccine vector for other pathogens. The rVSV/HIV-1 vaccine vector was attenuated by combining two major genome modifications. These modifications acted synergistically to greatly enhance vector attenuation and the resulting rVSV vector demonstrated safety in sensitive mouse and non-human primate neurovirulence models. This vector expressing HIV-1 gag protein has completed evaluation in two Phase I clinical trials. In one trial the rVSV/HIV-1 vector was administered in a ...
Gene therapy - an advanced technique developed to insert or inject therapeutic genes into human cells - has shown some success in treating the disease. In a previous study, Xiao and co-investigators at State Key Laboratory of Biotherapy, and the Department of Thoracic Oncology Cancer Center, West China Hospital, Sichuan University, had used a gene therapy approach to induce cancer cell death. Their study found that Vesicular Stomatitis Virus Matrix Protein (VSVMP), when inserted into a cancer cell, compromises the cellular skeletal framework, which is made up of structural proteins. Cell death ensued as a consequence. In the current study, the research team further armed with VSVMP gene delivery vessel with Interleukin-12 (IL-12) - a protein known to recruit and switch on the cancer-killing functions of immune cells. The novel drug particles are based on Heparin-polyethyleneimine (HPEI) nanoparticles. To overcome the high toxicity and non-biocompatible nature of PEI, the team used a method to ...
Gene therapy - an advanced technique developed to insert or inject therapeutic genes into human cells - has shown some success in treating the disease. In a previous study, Xiao and co-investigators at State Key Laboratory of Biotherapy, and the Department of Thoracic Oncology Cancer Center, West China Hospital, Sichuan University, had used a gene therapy approach to induce cancer cell death. Their study found that Vesicular Stomatitis Virus Matrix Protein (VSVMP), when inserted into a cancer cell, compromises the cellular skeletal framework, which is made up of structural proteins. Cell death ensued as a consequence. In the current study, the research team further armed with VSVMP gene delivery vessel with Interleukin-12 (IL-12) - a protein known to recruit and switch on the cancer-killing functions of immune cells. The novel drug particles are based on Heparin-polyethyleneimine (HPEI) nanoparticles. To overcome the high toxicity and non-biocompatible nature of PEI, the team used a method to ...
Upon infection with many different viruses, plasmacytoid dendritic cells (pDC) produce large amounts of type I interferon (IFN-alpha/beta). To address why upon vesicular stomatitis virus (VSV) infection pDC, but not conventional myeloid DC (mDC), are induced to produce IFN-alpha, pDC and mDC were differentiated from bone marrow cells (BM-DC). Upon VSV infection BM-pDC produced IFN-alpha, whereas BM-mDC did not. Notably, upon infection with VSV-M2, a VSV variant expressing a M51R mutant matrix (M) protein that showed a reduced sequestration of host cell metabolism, BM-pDC and BM-mDC mounted massive IFN-alpha responses. Both DC subsets showed comparable RNA levels of retinoic acid inducible gene-I (RIG-I) and Toll-like receptor (TLR) 7 and were able to respond upon triggering with double-stranded RNA (dsRNA) or single-stranded RNA (ssRNA) analogs. Moreover, upon VSV-M2 infection IFN-alpha production by both DC subsets was largely dependent on viral replication. Interestingly, upon virus infection BM-pDC,
Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells
Disease Information. Vesicular stomatitis is a viral disease which primarily affects horses, cattle, and swine. The agent that causes vesicular stomatitis, VSV, has a wide host range and can occasionally infect sheep and goats. In affected livestock, VSV causes blister-like lesions to form in the mouth and on the dental pad, tongue, lips, nostrils, hooves, and teats. These blisters swell and break, leaving raw tissue that is so painful that infected animals generally refuse to eat and drink and show signs of lameness. Severe weight loss usually follows, and in dairy cows a severe drop in milk production commonly occurs. Affected dairy cattle can appear to be normal and will continue to eat about half of their feed intake.. ...
Vesicular Stomatitis ("VS") is a viral disease that affects horses, and less commonly cattle, pigs, llamas, alpacas, and other livestock. We see periodic outbreaks of Vesicular Stomatitis in our region of the Southwest. VS is a reportable disease, meaning that when a case is suspected by a veterinarian, we are required to involve the United States Department of Agriculture: Animal and Plant Health Inspection Service (USDA: APHIS).. Reporting is required because VS resembles "Foot and Mouth Disease" in cattle, which is greatly feared in the livestock industry. When VS is confirmed in the United States, non-affected states and most foreign countries initiate transport embargoes to prevent spread into their territories. Movement of livestock is hindered and affected premises are quarantined. The entire livestock industry is adversely affected. After reporting a potential occurrence of the disease the animals in question must be inspected by USDA:APHIS. Laboratory work is performed to determine ...
Viral vectors have been available in various fields such as medical and biological research or gene therapy applications. Targeting vectors pseudotyped with distinct viral envelope proteins that influence cell tropism and transfection efficiency is a useful tool not only for examining entry mechanisms or cell tropisms but also for vaccine vector development. Vesicular stomatitis virus (VSV) is an excellent candidate for development as a pseudotype vector. A recombinant VSV lacking its own envelope (G) gene has been used to produce a pseudotype or recombinant VSV possessing the envelope proteins of heterologous viruses. These viruses possess a reporter gene instead of a VSV G gene in their genome, and therefore it is easy to evaluate their infectivity in the study of viral entry, including identification of viral receptors. Furthermore, advantage can be taken of a property of the pseudotype VSV, which is competence for single-round infection, in handling many different viruses that are either difficult
TY - JOUR. T1 - Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, and vesicular stomatitis virus. AU - Bosselman, Robert A.. AU - Price, Joseph A.. AU - Lee Burns, A.. AU - Kaulenas, Mindaugas S.. AU - Norkin, Leonard C.. PY - 1978/1/1. Y1 - 1978/1/1. N2 - The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the presence of one additional protein associated with T-22 monosomes.. AB - The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the ...
The presence of sulphate groups on various saccharide residues of N-linked carbohydrate units has now been observed in a number of glycoproteins. To explore the cell specificity of this post-translational modification, we evaluated sulphate incorporation into virus envelope glycoproteins by a variety of cells, since it is believed that assembly of their N-linked oligosaccharides is to a large extent dependent on the enzymic machinery of the host. Employing the vesicular stomatitis virus (VSV) envelope glycoprotein (G protein) as a model, we noted that the addition of [35S]sulphate substituents into its complex carbohydrate units occurred in Madin-Darby canine kidney (MDCK), Madin-Darby bovine kidney, LLC-PK1 and BHK-21 cell lines but was not detectable in BRL 3A, BW5147.3, Chinese hamster ovary, HepG2, NRK-49F, IEC-18, PtK1 or 3T3 cells. The sulphate groups were exclusively located on C-3 of galactose [Gal(3-SO4)] and/or C-6 of N-acetylglucosamine [GlcNAc(6-SO4)] residues in the ...
Plays a major role in assembly and budding of virion. Condensates the ribonucleocapsid core during virus assembly. Shut off cellular transcription by inhibiting mRNA nuclear export through direct interaction with host RAE1-NUP98 complex. This shutoff presumably inhibits interferon signaling and thus establishment of antiviral state in virus infected cells. Induces cell-rounding, cytoskeleton disorganization and apoptosis in infected cell (By similarity).
Define gonococcal stomatitis. gonococcal stomatitis synonyms, gonococcal stomatitis pronunciation, gonococcal stomatitis translation, English dictionary definition of gonococcal stomatitis. n. Inflammation of the mucous tissue of the mouth. n inflammation of the mouth stomatitic adj n. inflammation of the mouth. Noun 1. stomatitis -...
Early biochemical experiments established that the minimal RNA synthesis machinery of NNS RNA viruses comprises the N encased genomic RNA associated with the viral polymerase, an L-P complex (Emerson and Yu, 1975; Mellon and Emerson, 1978). The atomic structure of N‐RNA complexes from VSV and rabies virus provided evidence that the RNA must somehow be dissociated from N for copying by the polymerase (Albertini et al, 2006; Green et al, 2006). The co‐crystal structure of the PCTD of VSV with the N‐RNA complex led to a model in which P brings L to the RNA template by binding directly between N molecules, and this interaction is perhaps also required to keep L associated with the N‐RNA during copying (Green and Luo, 2009). By now providing the first direct evidence that L can actually use RNA in the absence of the N and P, we have defined the minimal RNA synthesis components as L and RNA. We conclude that while N and P play important roles in viral RNA synthesis they are not essential for ...
To localize the virus, we used a green fluorescent reporter gene coupled to the VSV-G gene in the position of the fifth VSV gene. This would shift the viral L-gene to the sixth position, resulting in attenuated L-protein synthesis and a slight reduction in replication (Dalton and Rose, 2001), an advantage when considering treatment of the brain. Live microscopic imaging of the brain allowed us for the first time to follow the time course with single-cell resolution, from before inoculation to a point when VSV had spread throughout the tumor. In experiments in which we imaged VSV oncolysis through a glass window above the brain using time-lapse confocal laser microscopy, green viral infection of red cortical tumors spread throughout the tumor, mostly by local expansion of the area of infected cells. Blood vessels appeared mostly undamaged even late in tumor infection. Furthermore, vessel cells that stained positive for the endothelial marker von Willebrand factor appeared to be spared from ...
Highly pathogenic viruses with zoonotic potential such as H5N1 influenza viruses, Nipah virus, rabies virus, SARS coronavirus, Lassa fever virus, and Ebola virus require handling in BSL-3/4 containment, which makes diagnosis of and studies on these viruses difficult and expensive. Propagation-incompetent pseudotype viruses represent an elegant solution for this problem. Pseudotype viruses are equipped with the envelope proteins of heterotypic viruses and therefore behave similar to these in terms of cell tropism or antibody-mediated neutralization.. Vesicular stomatitis virus (VSV) is known to incorporate foreign viral glycoproteins in a more or less unspecific manner, in particular in the absence of the VSV G glycoprotein. Pseudotype virus can be generated by propagating the glycoprotein-deficient mutant VSVΔG on cells that express the viral envelope protein of interest. The virus particles released are capable of performing a single round of infection, which is mediated by the incorporated ...
Several states including Florida, Kentucky, Illinois, North Carolina, and Oklahoma have enhanced the entry requirements for Texas livestock, including horses, due to the cases of vesicular stomatitis.
The New Mexico Livestock Board has issued a new directive regarding Vesicular Stomatitis (VS), signed by state veterinarian Dr. Dave Fly.. Read the rest of the story New Mexico Horse Breeders Association ...
Vaccines based on viruses have the ability to provide lifelong protection from disease through induction of robust immunological memory. The Rose laboratory has developed vaccine platforms based on recombinant viruses that can be engineered to express high levels of foreign antigens. One of the major platforms is based on recombinant vesicular stomatitis virus (VSV), a cattle virus that induces potent immune responses in a wide variety of animal species. The virus has been attenuated so that it no longer causes disease and then engineered to express protective antigens from other viruses or bacteria. Immunization with such vectors protects animals from infection and disease caused by numerous pathogens including influenza virus, respiratory syncytial virus, simian immunodeficiency virus (SIV), Ebola virus, and Yersinia pestis, the bacterium that caused the notorious bubonic plagues.. Research projects in the Rose laboratory are focused on further development and testing of the VSV vaccine ...
Plasmid pHEF-VSVG from Dr. Sergey Kasparovs lab contains the insert Vesicular Stomatitis Virus Glycoprotein and is published in Physiol Genomics. 2003 Feb 6. 12(3):221-8. This plasmid is available through Addgene.
The Herpes Stomatitis is also commonly referred to as Stomatitis Herpetic. The condition is a viral infection of the mouth which triggers both inflammation and ulcers. The mouth ulcers are not akin to canker sores that are triggered by a dissimilar virus. What Causes Herpes Stomatitis? Herpes Stomatitis is a transmissible virus disease that is […]. ...
Nicotinic stomatitis is not considered a pre cancerous condition, however there are exceptions to every rule. The image above shows nicotinic stomatitis in an elderly man who has been smoking a pipe for many years. He does not have teeth, nor does he have a denture to protect his palate. The thick, white keratinization on the edentulous (toothless) ridges are probably at least partly due to years of chewing on bare gums, made worse by the pipe smoke which was probably habitually aimed more at this area of the mouth than others. The irregular red and white lesion proved to be squamous cell carcinoma. While nicotinic stomatitis is not considered to be pre cancerous, leukoplakia definitely is! (See the images below.) Both leukoplakia and nicotinic stomatitis are composed of keratinized tissue, and the difference in carcinogenicity may, in fact, be due mostly to the differences in the resistances of the tissues on which they are found. Perhaps very long exposure of the palatal tissues to hot, ...
Creative Biolabs provides Human anti-vesicular stomatitis virus octapeptide (VSV8) (aa 52-59) T cell receptor, pCDTCR1 product for Biopharmaceutical research,preclinical and clinical trials.
Although viruses are the smallest organisms with the shortest genomes, they have major impacts on human health, causing deadly diseases (e.g., influenza, AIDS, cancer) on a global scale. To reproduce, a virus particle must infect a living cell and divert biosynthetic resources toward production of virus components. A better mechanistic understanding the infection cycle could lead to insights for more effective anti-viral strategies. However, simulating the virus infection cycle is a computationally hard problem because some species fluctuate rapidly while others change gradually in number. We study vesicular stomatitis virus, an experimentally accessible virus for which we have developed a deterministic kinetic model of growth (Lim et al, PLoS Comp Bio, 2006). Stochastic simulation of VSV genome encapsidation is a computationally-intensive chain reaction that produces rapid fluctuations in the nucleocapsid(N) protein that associates with the genome. Analytical results from order statistics ...
Parametric models are of limited use unless values of the model parameters are estimated. My research focuses on the estimation of parameters and confidence intervals for the both stochastic and deterministic viral models using experimental data obtained in Yin Group. Yin Group performs experiments to study and understand viral spread and replication of viruses like Influenza A and Vesicular Stomatitis Virus (VSV). The process of modeling and parameter estimation is a challenging problem for two reasons -- (a) relatively sparse experimental data does not allow parameter estimation in detailed mechanistic models, and (b) parameter estimation in stochastic models is computationally expensive. I attempt to solve these problems by building mathematical (stochastic and deterministic) models of reasonable size which allow parameter estimation and developing new approaches to perform stochastic parameter estimation with much less computational cost ...
Javascript is disabled in this browser. This page requires Javascript. Modify your browsers settings to allow Javascript to execute. See your browsers documentation for specific instructions ...
Cytoplasmic Compartmentalization of the Protein and Ribonucleic Acid Species of Vesicular Stomatitis Virus: The cytoplasmic sites of synthesis in L cells of the
Is Stomatitis a common side effect of Biotene? View Stomatitis Biotene side effect risks. Female, 82 years of age, was diagnosed with dry mouth and took Biotene . Patient was hospitalized.
Doctor answers on Symptoms, Diagnosis, Treatment, and More: Dr. Fisher on treatments for stomatitis: Eczema is an inflammatory condition of the skin that is in the allergy-family of reactions. Most commonly, eczema is treated with topical steroid creams or ointments, starting at the milder strengths and working our way up as needed. See your doctor to confirm the diagnosis and to discuss a treatment plan. for topic: Treatments For Stomatitis
Care guide for Tobacco Stomatitis (Ambulatory Care). Includes: possible causes, signs and symptoms, standard treatment options and means of care and support.
Plasmid pCAG-VSVG from Dr. Arthur Nienhuiss lab contains the insert VSV G protein and is published in Unpublished This plasmid is available through Addgene.
Biscuit underwent full mouth extraction in a single session, and all surgical flaps were closed with 5-0 Monocryl in a simple interrupted pattern (Figure 2A). Six weeks later, the degree of oral inflammation was significantly improved (Figure 2B). Biscuit had gained 1.5 pounds since the procedure, and his owners reported that Biscuit was "like a new cat." All medications were discontinued and Biscuit appears to be a clinical cure after extractions. ...
口腔炎(stomatitis)是一種疾病,指口腔黏膜或舌黏膜发生的炎症。. 該病的成因可以分為與口部有關及與全身有關,前者包括口部衛生欠佳、裝得不妥當的假牙、或是由灼熱食物造成的燙傷;後者則包括藥物反應、過敏或感染。. 涉及齒齦發炎的口腔炎稱為齒齦口腔炎。. ...
Vesicular stomatitis virus of the New Jersey serotype (VSV-NJ) causes vesicular disease in cattle, pigs, and horses throughout the Americas. Vesicular disease is clinically indistinguishable from foot-and-mouth disease (FMD). Therefore, outbreaks of vesicular disease in FMD-free areas must be rapidly diagnosed by laboratory methods and affected farms must be quarantined until laboratory results confirm the absence of FMD. Diagnosis is currently performed in high-containment (biosafety level 3) laboratories by using complement fixation and virus isolation in tissue culture. We describe here an alternative method for the detection of VSV-NJ RNA in clinical samples. This method includes a rapid acid guanidine-phenol RNA extraction procedure coupled with a one-tube polymerase chain reaction (PCR) using reverse transcriptase. By using this test, we were able to detect the largest number of positive samples (53 of 58), followed by complement (48 of 58) and isolation in tissue culture (43 of 58). The ...
A full-length cDNA copy of the phosphoprotein (NS) mRNA of vesicular stomatitis virus (New Jersey serotype) was inserted into pGEM4 vector downstream of the promoter for bacteriophage SP6 RNA polymerase. Transcription of the cDNA in vitro resulted in the synthesis of NS mRNA, which was subsequently translated into NS protein in a cell-free rabbit reticulocyte system. The biological activity of the expressed NS protein was demonstrated by in vitro synthesis of mRNA by transcription-reconstitution with purified viral L protein and N-RNA template. Deletion mapping of the NS gene defined a specific domain between amino acid residues 213 and 247, which was essential for in vitro transcription. Removal of the COOH-terminal 21 amino acids, on the other hand, did not have a significant effect on transcription. This domain appears to be involved in efficient binding of NS protein to the N protein-RNA template. ...
VSIV is an arbovirus. Natural VSIV infections encompass two steps, cytolytic infections of mammalian hosts and transmission by insects. In insects, infections are noncytolytic persistent. One confirmed vector of the virus is the phlebotomine sand fly Lutzomyia shannoni.[3] Vesicular stomatitis Indiana virus (VSIV) is the prototypic member of the genus Vesiculovirus of the family Rhabdoviridae. The genome of the virus is a single molecule of negative-sense RNA that encodes five major proteins: G protein (G), large protein (L), phosphoprotein, matrix protein (M) and nucleoprotein. The genome is 11,161 nucleotides long.[4] The VSIV G protein enables viral entry. It mediates viral attachment to an LDL receptor (LDLR) or an LDLR family member present on the host cell.[5] Following binding the VSIV-LDLR complex is rapidly endocytosed It then mediates fusion of the viral envelope with the endosomal membrane. VSIV enters the cell through partially clathrin-coated vesicles; virus-containing vesicles ...
Search the ASPR TRACIE Resource Library and view tailored Topic Collections comprised of current healthcare system preparedness resources.