The purpose of this research was to study the morphological features of cells and test the porcine follicular fluid (pFF) supplements in Vero cell lines culture. The pFF in the estrous cycle was collected and kept sterile properly. The fluid was categorized into three types: small size (1-3 mm), medium size (4-6 mm) and large size (,7 mm) follicles. In the control group, Vero cell lines were cultured in dulbeccos modified eagle medium (DMEM). In the positive control group, Vero cell lines were cultured in DMEM and supplemented using 10% heat-treated fetal bovine serum (HTFBS). In the third group, Vero cell lines were cultured in DMEM and supplemented using pFF from small, medium and large sized ovarian follicles at 2, 4, 20, 40, 200, 400, 500 and 600 μg protein/mL concentrations for 24 h, using MTT assay. The results related to pFF from small-, medium- and large-sized follicles indicated that the percentage of cell viability in tested group at 400-600 µg protein/mL showed the percentage of ...
BioAssay record AID 216212 submitted by ChEMBL: Evaluated in vitro for antiviral activity in vero cell cultures against Sindbis virus.
Parasite international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology
Video articles in JoVE about encephalitis virus western equine include Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors, Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay, Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses, Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids, In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus, Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems, Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain, High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum, High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale
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Elements of the cytoskeletal and microtubule networks were imaged in the Vero cell culture illustrated in this section by treating the fixed and permeabilized cells with mouse anti-alpha-tubulin primary antibodies, followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568.
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Before DNA testing is done, mosquito samples sent to the Texas Department of State Health Services will be run through a Vero cell culture. In this method mosquito juice is squeezed onto a specially prepared plate with a thin layer of living cells. After a few days of incubating the cells with the mosquito juice, a technician can look at the cells under magnification and determine if they look healthy. The presence of sick cells tells the technician that the mosquito pool carried one of several viruses that attack these cells. The health department prefers the Vero cell culture method because it is sensitive to many kinds of mosquito borne viruses. When a sample is positive for virus, then one of the molecular tests for specific virus RNA is run. These tests are highly specific, so that a test for WNV will not detect say St. Louis encephalitis (SLE) or chikungunya (CHIK), other important mosquito-borne diseases. Several tests will be run on any cell culture positive sample of mosquito juice. The ...
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BioAssay record AID 336244 submitted by ChEMBL: Antiviral activity against 10-3 MOI HSV1 infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect.
Statement of the Problem: Parvoviral diseases are emerging as a constant threat to penaeid culture due to their ability to cause slow growth and mass mortality...
Pak1 is required for ASFV entry in Vero cells.A) ASFV activates Pak1 at early times post infection. Cells were infected (MOI 5) and phosphorylation of Pak1 (Thr
Does anyone has ever heard of macropinocytosis events or macropinocytosis stimulators usefull with Vero cells? Thanxs Rose-may Delrue URBM / FUNDP Lab Immunol. & Microbiol. 61, rue de Bruxelles B-5000 Namur Belgium ...
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Bio-imaging and drug carriers for delivery have created a huge demand for crystals. Crystals are fascinating materials that have been grown for a long time but obtaining biocompatible fluorescent crystals is a challenging task. We report on the growth of fluorescent crystals using a carbon dot (C-dot) solution by a hydrothermal process. The crystallization pattern of these C-dots exhibited a unique dendritic structure having a feather-like morphology. The growth temperature and pressure were maintained at 60℃ and 200 mmHg, respectively, for crystal growth. A green fluorescence (under UV light) that was observed in the C-dot solution was retained in the crystals formed from the solution. Cytotoxicity studies on Vero cells revealed the crystals to be extremely biocompatible. These fluorescent crystals are extremely well suited for biomedical and optoelectronic applications. ...
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TY - JOUR. T1 - Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes. AU - Beasley, David W.C.. AU - Morin, Merribeth. AU - Lamb, Ashley R.. AU - Hayman, Edward. AU - Watts, Douglas M.. AU - Lee, Cynthia K.. AU - Trent, Dennis W.. AU - Monath, Thomas P.. PY - 2013/9/1. Y1 - 2013/9/1. N2 - Serial passaging of yellow fever virus 17D in Vero cells was employed to derive seed material for a novel inactivated vaccine, XRX-001. Two independent passaging series identified a novel lysine to arginine mutation at amino acid 160 of the envelope protein, a surface-exposed residue in structural domain I. A third passage series resulted in an isoleucine to methionine mutation at residue 113 of the NS4B protein, a central membrane spanning region of the protein which has previously been associated with Vero cell adaptation of other mosquito-borne flaviviruses. These studies confirm that flavivirus adaptation to growth in Vero cells can ...
in Journal of Virology (1993), 67(7), 4379-4385. Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a protein with a predicted molecular weight of 51,540 presenting amino acid sequence homology with the immediate-early regulatory protein ... [more ▼]. Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a protein with a predicted molecular weight of 51,540 presenting amino acid sequence homology with the immediate-early regulatory protein ICP27 of herpes simplex virus type 1. To investigate the regulatory properties of the ORF4 gene product, we performed a series of transient expression assays in Vero cells, using a plasmid expressing ORF4 as effector and several VZV genes and heterologous genes as targets. The VZV target plasmids contained promoter/regulatory regions from genes belonging to the three putative VZV kinetic classes fused to the chloramphenicol acetyltransferase (CAT) gene. The heterologous target plasmids consisted of promoter/regulatory regions of ...
Two Cellmatics (Difco) Herpes simplex virus (HSV) detection systems, one with mink lung cells (ML) and the other with primary rabbit kidney cells (PRK) and the Cultureset (Ortho) system with Vero cells were compared for their ability to detect previously positive specimens. One hundred fifty patients specimens positive for HSV in either Vero or PRK cells prior to freezing at -70 degrees C were thawed once, diluted to 1:8, and inoculated in duplicate into each cell system. Positive cultures were detected using each kits immunoperoxidase method. All three cell lines were stained at 24 hr followed by a final staining of negative cultures at 48 hr for Cultureset and at 72 hr for Cellmatics as per kit instructions. At 24 hr percent detection was: Cellmatics (ML) = 85%, Cellmatics (PRK) = 81%, Cultureset (Vero) = 74%. At final staining percent detection was 94%, 92%, and 84%, respectively. The Cellmatics system has the advantage of fewer steps in the staining procedure and no manipulation of coverslips
According to Mikovits, one contaminated cell line is the Vero monkey kidney cell line called Vero E6, which was given by Fort Detrick - a U.S. Army Medical Command installation that hosts many of our national biological defense programs and houses the National Cancer Institute laboratory where she used to work - to the biosafety 4 laboratory (BSL-4) in Wuhan, China. This cell line is what the Wuhan lab used to grow and study coronaviruses, she says.. The Vero cell line is listed in the 2015 paper,8 "A SARS-like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence," co-written by University of North Carolina researchers and Dr. Shi Zhengli, a Chinese virologist at the Wuhan lab who in 2010 published a paper9 discussing the weaponization of the SARS virus.. The contaminated Vero monkey kidney cells were also used in the production of polio vaccines, Mikovits notes. The original polio vaccines were passed through mice brains, as we didnt have cell lines in the 1930s when ...
De novo and reference-guided assembly.De novo assembly of the short reads was performed to generate new HSV-1 genomes from the sequence data, followed by a reference-guided assembly of the resulting blocks of contiguous sequences, or contigs. The short sequence reads were first passed through a series of computational filters that removed (i) mononucleotide sequences, (ii) host sequence contamination, and (iii) low-quality sequence. For step i, sequences that consisted of a single nucleotide or a single nucleotide with some N (noncalled) bases were removed. (Step ii) Since virus stocks were prepared on Vero cells, it was critical to identify and remove host DNA sequences. Because the vervet monkey (Vero cell parent) genome sequence is not known, the sequence data were mapped to the human genome (version 36) using the Mapping and Alignment with Qualities (MAQ) software package (32). Sequences homologous to human DNA varied from 0.2 to 15% of the data (see Table S1 in the supplemental material); ...
Theodore G. Andreadis is the author of this article in the Journal of Visualized Experiments: Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
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