TY - JOUR. T1 - Coffee induces vascular endothelial growth factor (VEGF) expression in human neuroblastama SH-SY5Y cells. AU - Kakio, Shota. AU - Tago, Megumi. AU - Kobata, Kenji. AU - Tamura, Hiroomi. PY - 2016/1/21. Y1 - 2016/1/21. N2 - Recent evidence indicates that hypoxia-inducible vascular endothelial growth factor (VEGF) has neurotrophic and neuroprotective effects on neuronal and glial cells. On the other hand, recent epidemiological studies showed that daily coffee consumption has been associated with a lower risk of several neuronal disorders. Therefore, we investigated the effect of coffee on VEGF expression in human neuroblastoma SH-SY5Y cells. We found that even low concentration of coffee (. AB - Recent evidence indicates that hypoxia-inducible vascular endothelial growth factor (VEGF) has neurotrophic and neuroprotective effects on neuronal and glial cells. On the other hand, recent epidemiological studies showed that daily coffee consumption has been associated with a lower risk ...
Table of Contents. Table of Contents 2. List of Tables 5. List of Figures 5. Introduction 6. Global Markets Direct Report Coverage 6. Placenta Growth Factor (Vascular Endothelial Growth Factor Related Protein or PGF) Overview 7. Therapeutics Development 8. Placenta Growth Factor (Vascular Endothelial Growth Factor Related Protein or PGF)-Products under Development by Stage of Development 8. Placenta Growth Factor (Vascular Endothelial Growth Factor Related Protein or PGF)-Products under Development by Therapy Area 9. Placenta Growth Factor (Vascular Endothelial Growth Factor Related Protein or PGF)-Products under Development by Indication 10. Placenta Growth Factor (Vascular Endothelial Growth Factor Related Protein or PGF)-Pipeline Products Glance 11. Late Stage Products 11. Early Stage Products 12. Placenta Growth Factor (Vascular Endothelial Growth Factor Related Protein or PGF)-Products under Development by Companies 13. Placenta Growth Factor (Vascular Endothelial Growth Factor Related ...
Background: The development of biomarkers predictive of response to immune checkpoint inhibitor (ICI) therapies in advanced melanoma is an area of great interest in oncology. Our study evaluated the potential role of serum vascular endothelial growth factor (VEGF) as a predictive biomarker of clinical benefit and response to treatment with ICIs.Methods: Pre-treatment peripheral blood samples were obtained from advanced melanoma patients undergoing ICI therapy as monotherapy or in combination at two tertiary care hospitals in Western Australia. Serum VEGF levels were correlated with response to therapy and survival outcomes.Results: Serum VEGF samples were collected from a total of 130 patients treated with ICI therapy (pembrolizumab 73, ipilimumab 15, and ipilimumab/nivolumab combination 42). Median serum VEGF level was significantly higher in the non-responders (82.15 pg/mL) vs. responders (60.40 pg/mL) in the ipilimumab monotherapy cohort (P Conclusions: The results of our study confirm previous
TY - JOUR. T1 - Role of copper and vascular endothelial growth factor (VEGF) on endometrial angiogenesis. AU - Chianeh, Yousef Rezaei. AU - Rao, Pragna. PY - 2013. Y1 - 2013. N2 - The formation of new blood vessels is the initial step in neovascularisation. The first stage in angiogenesis is the activation of endothelial cells. Copper ions stimulate proliferation and immigration of endothelial cells. It has been shown that serum copper concentration increases as the cancer disease progresses and correlates with tumour incidence and burden. Copper ions also activate several proangiogenic factors, e.g., vascular endothelial growth factor, basic fibroblast growth factor, and interleukin 1. This review concerns a brief introduction into the basics of blood vessel development as well as the regulatory mechanisms of this process. The role of copper ions in angiogenesis is discussed.. AB - The formation of new blood vessels is the initial step in neovascularisation. The first stage in angiogenesis is ...
Title: Vascular Endothelial Growth Factor (VEGF) in the Pathogenesis of Diabetic Nephropathy of Type 1 Diabetes Mellitus. VOLUME: 12 ISSUE: 1. Author(s):Maria Mironidou-Tzouveleki, Stergios Tsartsalis and Constantinos Tomos. Affiliation:A Laboratory of Pharmacology, Medical School, Aristotle University of Thessaloniki, Komninon 4, 57010, Filyro, Greece.. Keywords:Nephropathy, pathogenesis, type 1 diabetes mellitus, VEGF, Diabetic nephropathy, Vascular endothelial growth factor, VEGFRs, hypertrophy, PlGF, angiogenesis, Rac, sFlt-1, glomerular endotheliosis, proteinuria, hypertension, glomerulosclerosis, glomerular filtration rate (GFR), Denys-Drash syndrome, nephritic syndrome, renal hypoxia, HIF, ERK, ROS, AGEs, albuminuria, microalbuminuria, CTAD, ELISA, Ranibizumab, Pegaptanib, Aflibercept, VEGF Trap-Eye, angiotensin converting enzyme (ACE). Abstract: Diabetic nephropathy (DN) is a common complication of diabetes mellitus and is the primary cause of endstage renal disease in the Western ...
Angiogenesis, the growth of blood vessel from the existing vasculature, depends on the expression of various growth factors but Vascular Endothelial Growth factor (VEGF) ranks as the key inducer and the central mediator that promotes vascular permeability.
Vascular endothelial growth factor (VEGF) is definitely 1 of the many essential angiogenic growth factors for tumor angiogenesis. growth angiogenesis in MMP-2 suppressed growth areas was associated with decreased co-localization of MMP-2 and integrin-V3. In overview, these data offer fresh information into the systems root MMP-2-mediated VEGF appearance in lung growth angiogenesis. and versions. Our results reveal that MMP-2 transcriptional inactivation decreased integrin-V3-mediated considerably, PI3E/AKT-induced VEGF appearance, which reduced tumor cell-induced angiogenesis ultimately. Components AND Strategies Cells and Reagents A549 and L1299 lung adenocarcinoma cells had been cultured in RPMI 1640 (ATCC Manassas, Veterans administration) supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, California), 50 devices/mL penicillin, and 50 g/mL streptomycin (Existence Systems, Inc., Frederick, MD). For human being microvascular skin endothelial cells (HMEC-1), glutamine, EGF buy ...
This study will look for the presence in blood of a substance called vascular endothelial growth factor (VEGF) in patients with uveitis (eye inflammation). It will also look for this substance in eye fluid samples taken from patients with uveitis who are undergoing eye surgery.. Some patients with uveitis experience some vision loss during an inflammatory attack because of swelling (edema) in a particular area of the retina called the macula, which is involved in visual acuity. It may be that VEGF is involved in the development of macular edema.. Patients with uveitis who participate in this study will have about 10 cc (2 teaspoons) of blood drawn to be examined for VEGF. They will also undergo a procedure called fluorescein angiography to look at the blood vessels of the eye. A dye called sodium fluorescein is injected into the blood stream through a vein. After the dye reaches the blood vessels of the eye, photographs are taken of the retina.. In addition, patients with uveitis who are ...
Yasuhara, T., Shingo, T., Kobayashi, K., Takeuchi, A., Yano, A., Muraoka, K., Matsui, T., Miyoshi, Y., Hamada, H. and Date, I. (2004), Neuroprotective effects of vascular endothelial growth factor (VEGF) upon dopaminergic neurons in a rat model of Parkinsons disease. European Journal of Neuroscience, 19: 1494-1504. doi: 10.1111/j.1460-9568.2004.03254.x ...
Prognostic significance of plasma vascular endothelial growth factor levels in patients with hormone-refractory prostate cancer treated on Cancer and Leukemia Group B 9480.
TY - JOUR. T1 - Anthrax lethal toxin inhibits growth of and vascular endothelial growth factor release from endothelial cells expressing the human herpes virus 8 viral G protein-coupled receptor. AU - Depeille, Philippe. AU - Young, John J.. AU - Boguslawski, Elissa A.. AU - Berghuis, Bree D.. AU - Kort, Eric J.. AU - Resau, James H.. AU - Frankel, Arthur E.. AU - Duesbery, Nicholas S.. PY - 2007/10/1. Y1 - 2007/10/1. N2 - Purpose: In this study, we tested the hypothesis that inhibition of mitogen-activated protein kinase kinases (MKK) inhibits tumor growth by acting on angiogenic signaling and by extension may form the basis of an effective strategy for treatment of Kaposis sarcoma. Experimental Design: Murine endothelial cells expressing the human herpes virus 8 G protein-coupled receptor (vGPCR-SVEC) were treated with anthrax lethal toxin (LeTx). LeTx is a binary toxin ordinarily secreted by Bacillus anthracis and is composed of two proteins: protective antigen (the binding moiety) and ...
Title:Targeting Vascular Endothelial Growth Factor in Neuroblastoma. VOLUME: 2 ISSUE: 1. Author(s):Danielle Hsu, Jason M. Shohet and Eugene S. Kim. Affiliation:Division of Pediatric Surgery, Texas Childrens Hospital, CCC Building, Suite 1210, 6701 Fannin St, Houston, TX 77030, USA.. Keywords:Vascular endothelial growth factor, angiogenesis, clinical trials, neuroblastoma.. Abstract:Angiogenesis is critical to tumor growth and metastasis and is dependent on growth factors, such as vascular endothelial growth factor (VEGF). The most characterized angiogenic factor, VEGF is an endothelial cell mitogen and permeability factor that has been found to be overexpressed in almost all human cancers. In a number of tumor model systems, antagonism of the VEGF pathway results in inhibition of angiogenesis and tumor growth. Specifically, VEGF inhibition has been shown to suppress tumor growth, decrease microvasculature, and induce apoptosis of endothelial cells. This close relationship between hypoxia, ...
Angiogenesis, the formation of blood vessels, is essential for preparing a closed circulatory system in the body, and for supplying oxygen and nutrition to tissues. Major diseases such as cancer, rheumatoid arthritis, and atherosclerosis include pathological angiogenesis in their malignant processes, suggesting anti-angiogenic therapy to be a new strategy for suppression of diseases. However, until the 1970s, the molecular basis of angiogenesis was largely unknown. In recent decades, extensive studies have revealed a variety of angiogenic factors and their receptors, including vascular endothelial growth factor (VEGF)-VEGFRs, Angiopoietin-Tie, Ephrin-EphRs and Delta-Notch to be the major regulators of angiogenesis in vertebrates. VEGF and its receptors play a central role in physiological as well as pathological angiogenesis, and functional inhibitors of VEGF and VEGFRs such as anti-VEGF neutralizing antibody and small molecules that block the tyrosine kinase activity of VEGFRs have recently been
In recent years, vascular inflammation, marked by activated monocytes and endothelium, has been proven to play a significant role in the pathogenesis of vasoocclusive events (VOEs) in sickle cell disease (SCD). Vascular endothelial growth factor ( VEGF), an endothelial cell mitogen, has been shown to contribute to the increased endothelial cell adhesivity by increasing the expression of cell adhesion molecules ICAM-1 ( intercellular adhesion molecule-1) and VCAM-1 ( vascular cell adhesion molecule-1) on the endothelium. We have investigated VEGF alterations in 37 patients with SCD during VOEs and/or steady state. VEGF levels ( mean +/- SEM) were found to be significantly elevated during VOEs ( 703.1 +/- 119.0 pg/ml) when compared with those at steady state (258.0 +/- 57.8 pg/ml) and healthy controls (196.6 +/- 21.9 pg/ml) ( p< 0.001). However, we did not find a difference between VEGF concentrations in sickle patients at steady state and the normal subjects ( p> 0.05). We suggest that ...
TY - JOUR. T1 - Vascular endothelial growth factor is increased during early stage of diabetic nephropathy in type II diabetic rats. AU - Cha, Dae-Ryong. AU - Kang, Young Sun. AU - Han, Sang Youb. AU - Jee, Yi Hwa. AU - Han, Kum Hyun. AU - Han, Jee Young. AU - Kim, Young Sik. AU - Kim, Nan Hee. PY - 2004/10/1. Y1 - 2004/10/1. N2 - Vascular endothelial growth factor (VEGF) has been implicated in the pathogenesis of diabetic nephropathy. We investigated serial changes of VEGF in the kidney and assessed whether glomerular and urinary VEGF levels are related to the severity of diabetic nephropathy. Furthermore, we examined the relationship between urinary VEGF levels and the urinary albumin excretion (UAE) rate in Otsuka-Long-Evans-Tokushima-Fatty (OLETF) rats. Glomerular VEGF mRNA expression and protein synthesis were evaluated by the reverse transcription-polymerase chain reaction, immunohistochemical staining and in situ hybridization. Urinary levels of VEGF were determined by enzyme-linked ...
Vascular endothelial growth factor (VEGF) is a potent vascular permeability factor and a mediator of brain edema. To assess the role of vascular endothelial growth factor in eosinophilic meningitis, vascular endothelial growth factor was measured in the cerebrospinal fluid (CSF) and blood of 9 patients with eosinophilic meningitis in a cohort study. VEGFCSF was detected in 8 (90%) of 9 eosinophilic meningitis patients (range, 45-2190 pg/mL) at presentation. The mean VEGFCSF at presentation, 1 week, and 2 weeks after admission was 568 pg/mL, 751 pg/mL, and 1031 pg/mL, respectively. There was an association between VEGFCSF, CSF protein, white cell count, and eosinophil counts. The VEGFSERUM fluctuated during the 6-month follow-up period. These results indicate that vascular endothelial growth factor may be associated with blood-brain barrier disruption in patients with eosinophilic meningitis.
Vitreous levels of vascular endothelial growth factor in eyes with anterior hyaloidal fibrovascular proliferation Takaki Kobayashi, Shigeki Machida, Takamitsu Fujiwara, Tadashi Ishibe, Daijiro KurosakaDepartment of Ophthalmology, Iwate Medical University School of Medicine, Iwate, JapanPurpose: To determine the intravitreal levels of vascular endothelial growth factor (VEGF) in eyes with anterior hyaloidal fibrovascular proliferation (AHFVP).Methods: Three eyes of three patients who underwent vitrectomy for proliferative diabetic retinopathy (PDR) and subsequently developed an AHFVP (AHFVP group) were studied. We measured the level of VEGF in vitreous samples collected at the primary and following operations by enzyme-linked immunosorbent assay. The vitreous levels of VEGF in 25 eyes of 22 patients with PDR were also studied as controls (PDR group).Results: The averaged VEGF level in the samples collected at the primary surgery was 1.98 ± 2.23 ng/mL in the PDR group, and it was 9.07, 1.94, and 8
TY - JOUR. T1 - Vascular endothelial growth factor regulates excitatory synaptic transmission in hippocampal neurons. AU - Huang, Yu Fei. AU - Yang, Chih Hao. AU - Chiou, Chiuan Shiou. AU - Huang, Chiung Chun. AU - Hsu, Kuei Sen. PY - 2010. Y1 - 2010. N2 - Vascular endothelial growth factor (VEGF), in addition to its essential role in the processes of vascularization and angiogenesis, exerts direct effects on neural cells in the central nervous system. There is abundant evidence indicating that VEGF protects neurons against cell death induced by a variety of insults, including hypoxia/ischemia and seizures. Recent work has demonstrated the expression of VEGF and its receptors in neurons and has revealed that VEGF can act as a neurotrophic factor to regulate neurogenesis and mediate the effects of enriched environment and antidepressants on hippocampal plasticity. Current studies from our laboratory and those of others have found that VEGF can activate divergent signaling components to regulate ...
TY - JOUR. T1 - Vascular endothelial growth factor regulation of Weibel-Palade-body exocytosis. AU - Matsushita, Kenji. AU - Yamakuchi, Munekazu. AU - Morrell, Craig N.. AU - Ozaki, Michitaka. AU - ORourke, Brian. AU - Irani, Kaikobad. AU - Lowenstein, Charles J.. PY - 2005/1/1. Y1 - 2005/1/1. N2 - Vascular endothelial growth factor (VEGF) not only regulates angiogenesis, vascular permeability, and vasodilation but also promotes vascular inflammation. However, the molecular basis for the proinflammatory effects of VEGF is not understood. We now show that VEGF activates endothelial cell exocytosis of Weibel-Palade bodies, releasing vasoactive substances capable of causing vascular thrombosis and inflammation. VEGF triggers endothelial exocytosis in part through calcium and phospholipase C-γ (PLC-γ) signal transduction. However, VEGF also modulates endothelial cell exocytosis by activating endothelial nitric oxide synthase (eNOS) production of nitric oxide (NO), which nitrosylates ...
TY - JOUR. T1 - The Relationship of Vascular Endothelial Growth Factor and Coagulation Factor (Fibrin and Fibrinogen) Expression in Clear Cell Renal Cell Carcinoma. AU - Verheul, Henk M.W.. AU - van Erp, Karen. AU - Homs, Marjolein Y.V.. AU - Yoon, G. S.. AU - van Der Groep, Petra. AU - Rogers, Craig. AU - Hansel, Donna E.. AU - Netto, George J.. AU - Pili, Roberto. PY - 2010/3/1. Y1 - 2010/3/1. N2 - Objectives: To investigate the relationship between angiogenesis and coagulation markers in tumor tissues of primary renal cell carcinoma (RCC). Tumors stimulate angiogenesis and activate the coagulation cascade. The importance of the interplay between these pathways for RCC is unknown. Methods: In all, 69 clear cell RCC specimens were analyzed by immunohistochemical staining applied to tissue microarrays. The expression of vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1α, fibrinogen and fibrin, and microvessel density were visually analyzed. Finally, staining patterns were ...
TY - JOUR. T1 - Vascular endothelial growth factor as a biomarker for the early detection of cancer using a whole cell-based biosensor. AU - May, Kimberly M L. AU - Vogt, Adam. AU - Bachas, Leonidas G. AU - Anderson, Kimberly W.. PY - 2005/6. Y1 - 2005/6. N2 - Vascular endothelial growth factor (VEGF) is a cytokine and endothelial cell (EC) mitogen that has been studied for its role in angiogenesis of malignant tumors. Elevated quantities of VEGF in the serum and plasma of patients have been correlated with the presence of cancer and metastasis. Since VEGF induces hyperpermeability of EC monolayers, this protein can be detected in vitro with a whole cell-based biosensor. This biosensor consists of a confluent monolayer of human umbilical vein endothelial cells (HUVECs) attached to a cellulose triacetate (CTA) membrane of an ion-selective electrode (ISE). Previous studies regarding this biosensor have shown that when the biosensor was exposed to a model toxin, such as histamine, the response of ...
Up-regulation of vascular endothelial growth factor (VEGF) expression is a major event leading to neovascularization in malignant gliomas. Hypoxia is believed to be the crucial environmental stimulus for this up-regulation. To critically assess this hypothesis, we asked whether the mechanisms defined previously for hypoxia-induced VEGF expression in vitro are similarly involved and sufficient for up-regulation of VEGF gene expression in vivo, using a lacZ reporter gene under the control of VEGF regulatory sequences in an experimental glioma model. Inclusion of the binding site for hypoxia-inducible factor 1 (HIF 1) in the 5′ regulatory sequences used in the hybrid gene produced weak β-galactosidase staining in a special tumor cell subtype, the so-called perinecrotic palisading (PNP) cells that flank necrotic regions within the tumor. Deletion of the HIF 1 binding site abolished reporter gene expression in the PNP cells, indicating that transcriptional activation of VEGF expression in gliomas ...
TY - JOUR. T1 - Increased vascular endothelial growth factor transcription in residual hepatocellular carcinoma after open versus laparoscopic hepatectomy in a small animal model. AU - Perry, Kyle A.. AU - Enestvedt, Charles. AU - Hosack, Luke W.. AU - Pham, Thai H.. AU - Diggs, Brian S.. AU - Teh, Swee. AU - Orloff, Susan. AU - Winn, Shelly. AU - Hunter, John. AU - Sheppard, Brett. PY - 2010/5. Y1 - 2010/5. N2 - Background Vascular endothelial growth factor (VEGF) is overexpressed in hepatocellular carcinoma (HCC), and findings have shown that its upregulation in these tumors has an impact on tumor growth. The authors hypothesized that compared with open liver resection, laparoscopic hepatectomy would result in a decreased local angiogenic response in residual tumor cells. Methods Right- and left-lobe hepatomas were induced in Buffalo rats via laparoscopically guided subcapsular injection of Morris hepatoma cells. After 1 week, the animals were randomized to laparoscopic or open left lateral ...
The purpose of this study is to evaluate the activity of RO4929097 in renal cell carcinoma patients that have failed therapy with VEGF/VEGFR directed ag
98. Fraenzer, J.-Th., Wachs, F.P., Licht, K., Gleich, O., Strutz, J. (2006) Charakterisierung von Vascular Endothelial Growth Factor (VEGF) und seines Rezeptors Flk-1 in Zellkulturen aus dem Innenohr neugeborener Mäuse. 9. Jahrestagung der Deutschen Gesellschaft für Audiologie. p139. 99. Fraenzer, J.-Th., Wachs, F.P., Licht, K., Gleich, O., Strutz, J. (2006) Charakterisierung von Vascular Endothelial Growth Factor (VEGF) und seines Rezeptors Flk-1 in Zellkulturen aus dem Innenohr neugeborener Mäuse. Vortragskurzfassung 9. Jahrestagung der Deutschen Gesellschaft für Audiologie, Tagungs-CD ISBN 3-9809869-5-0. 100. Gleich, O., Fraenzer, J.-Th., Strutz, J., (2007) Preliminary data evaluating the effect of Gabapentin on auditory temporal resolution characterized by a gap detection task in gerbils. Abstr. 30th ARO Midwinter Res. Mtg. 316-317. 101. Gleich, O., Thurnbauer, K., Strutz, J., (2007) Differentielle altersbedingte Veränderungen in den Unterkernen des Nucleus cochlearis der ...
Edman degradation analyses and co-migration of synthetic peptides with EGFR-derived tryptic phosphopeptides identify these sites as Tyr845 and Tyr1101. Equilibrium population dynamics when mating is by mutual choice based on age. The silver grains do not 100mg viagra without a doctor prescription appear localized at postsynaptic level. The patient was discharged from hospital 18 days after treatment with NIHSS score 5. Maximal endothelial tissue plasminogen activator release is not impaired in patients with acute coronary syndromes before heparin treatment.. Temporal sequence of cell wall disassembly events in generic cialis developing fruits. Elderly trauma in a resource-poor area in sub-Saharan Africa is associated with a significant increase in hospital admissions and mortality. Other environmental changes may be needed to facilitate more thorough support. The directional transport of the phytohormone auxin represents a key, plant-specific mechanism for polarization and patterning in complex ...
Vascular endothelial growth factor (VEGF) functions as a regulator of neovascularization in malignant cells. VEGF as a mitogen is thought to alter renal cell carcinoma formation and tumor progression. We investigated the expression of the VEGF gene in order to evaluate its clinical significance in renal cell carcinoma. Tissue samples from 198 patients with renal cell carcinoma were examined with an immunohistochemical stain for the expression of the VEGF gene. The expression rate was compared to 34 normal renal cortical samples obtained from renal surgery from noncancer patients. There were significant differences between normal renal cortex (0%) and cancer tissue (54.5%) in positive staining of VEGF protein (P|0.001). With the progression of tumor grade, the positive rate of VEGF gene expression significantly increased. The expression rate of the VEGF gene in the advanced group, such as with lymph node involvement or vein invasion, was greater than that in the locally confined group (P|0.001). The
Previous work showed that connexin 43 (Cx43) reduced the expression of hypoxic-induced factor-1α (HIF-1α) in astrocytes. HIF-1α is a master transcription factor for angiogenesis in tumor. Angiogenesis is essential for tumor progression. Here, we investigated the role of Cx43 in vascular endothelial growth factor (VEGF) production and angiogenesis in murine tumor. In the study, mouse B16F10 and 4T1 cells were overexpressed or knockdown with Cx43. The expression profiles as well as activity of the treated cells were examined. Furthermore, reduced Cx43 expression in B16F10 and 4T1 cells causes increased expression of VEGF and enhanced the proliferation of endothelial cells. On the contrary, the expression of VEGF and the proliferation of endothelial were increased in the conditioned medium of Cx43-knockdown tumor cells. We subcutaneously transplanted Cx43-overexpressing B16F10 cells into mice to evaluate the roles of Cx43 in the tumor angiogenesis. Both tumor size and the number of vessels growing in
Tubulin-binding vascular-disrupting agents (VDA) are currently in clinical trials for cancer therapy but the factors that influence tumor susceptibility to these agents are poorly understood. We evaluated the consequences of modifying tumor vascular morphology and function on vascular and therapeutic response to combretastatin-A4 3-O-phosphate (CA-4-P), which was chosen as a model VDA. Mouse fibrosarcoma cell lines that are capable of expressing all vascular endothelial growth factor (VEGF) isoforms (control) or only single isoforms of VEGF (VEGF120, VEGF164, or VEGF188) were developed under endogenous VEGF promoter control. Once tumors were established, VEGF isoform expression did not affect growth or blood flow rate. However, VEGF188 was uniquely associated with tumor vascular maturity, resistance to hemorrhage, and resistance to CA-4-P. Pericyte staining was much greater in VEGF188 and control tumors than in VEGF120 and VEGF164 tumors. Vascular volume was highest in VEGF120 and control tumors ...
1. Klein R, Peto T, Bird A, Vannewkirk MR. The epidemiology of age-related macular degeneration. American journal of ophthalmology. 2004;137:486-95 2. Ambati J, Ambati BK, Yoo SH, Ianchulev S, Adamis AP. Age-related macular degeneration: etiology, pathogenesis, and therapeutic strategies. Survey of ophthalmology. 2003;48:257-93 3. van Wijngaarden P, Qureshi SH. Inhibitors of vascular endothelial growth factor (VEGF) in the management of neovascular age-related macular degeneration: a review of current practice. Clin Exp Optom. 2008;91:427-37 4. Ferrara N. Vascular endothelial growth factor and age-related macular degeneration: from basic science to therapy. Nat Med. 2010;16:1107-11 5. Kovach JL, Schwartz SG, Flynn HW Jr, Scott IU. Anti-VEGF Treatment Strategies for Wet AMD. J Ophthalmol. 2012;2012:786870 6. Ferrara N, Adamis AP. Ten years of anti-vascular endothelial growth factor therapy. Nat Rev Drug Discov. 2016;15:385-403 7. Park YG, Rhu HW, Kang S, Roh YJ. New Approach of Anti-VEGF Agents ...
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced ...
Angiogenesis is essential in physiological processes including ovulation, implantation and pregnancy. One of the most potent regulators is the cytokine vascular endothelial growth factor (VEGF). We provide evidence for a novel pregnancy-associated soluble variant of the VEGF receptor Flt-1. VEGF ranged from undetectable to 157.3 pg/ml (mean 49.9 pg/ml, SD 48.4 pg/ml) in plasma samples from normal volunteers (n = 10), but was undetectable in plasma from pregnant women (n = 12) and amniotic fluid (n = 10). Recoveries of spiked VEGF were poor in pregnancy-related samples, indicating the presence of VEGF-binding activity which was confirmed using biosensor and chromatographic techniques. Partial purification and protein sequencing indicated a novel soluble form of Flt-1 with a subunit size of 150 kDa. Normally present as a multimeric structure of approximately 400-550 kDa, complexes of 600-700 kDa were formed following binding of multiple VEGF molecules. Reverse transcriptase polymerase chain ...
Purpose This study was aimed to identify the expression pattern of vascular endothelial growth factor (VEGF) in non-small cell lung cancer (NSCLC) and to explore its potential correlation with the progression of NSCLC. Methods Gene expression profile GSE39345 was downloaded from the Gene Expression Omnibus database. Twenty healthy controls and 32 NSCLC samples before chemotherapy were analyzed to identify the differentially expressed genes (DEGs). Then pathway enrichment analysis of the DEGs was performed and protein-protein interaction networks were constructed. Particularly, VEGF genes and the VEGF signaling pathway were analyzed. The sub-network was constructed followed by functional enrichment analysis. Results Total 1666 up-regulated and 1542 down-regulated DEGs were identified. The down-regulated DEGs were mainly enriched in the pathways associated with cancer. VEGFA and VEGFB were found to be the initiating factor of VEGF signaling pathway. In addition, in the epidermal growth factor receptor
Steroid Responsive Meningitis-Arteritis (SRMA) is a common cause of inflammation of the canine central nervous system (CNS). To investigate if transforming growth factor beta 1 (TGF-β1), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) are involved in the production of excessive immunoglobulin A (IgA), the induction of acute phase proteins and in the development of a systemic necrotizing vasculitis, characteristic of SRMA, these three signalling proteins were evaluated. Cerebrospinal fluid (CSF) and serum samples of dogs during the acute phase of SRMA (SRMA) were tested for IL-6, VEGF and TGF- β1. Results were compared to those of dogs affected with SRMA during treatment (SRMA Th) and during relapse (SRMA R), to dogs with other meningoencephalomyelitides (ME), with miscellaneous non-inflammatory diseases of the CNS (CNS-Mix), with idiopathic epilepsy (IE), with systemic inflammatory diseases (Syst. Infl.) and with healthy dogs (Healthy). Concentrations of IL-6 and VEGF in CSF were
Increasing evidence suggests that cerebral vascular dysfunction is associated with the early stages of Alzheimers disease (AD). Vascular endothelial growth factor (VEGF) is one of the key players involved in the development and maintenance of the vasculature. Here, we hypothesized that VEGF levels in cerebrospinal fluid (CSF) may be altered in AD patients with vascular involvement, characterized by the presence of microbleeds (MB), and in vascular dementia (VaD) patients compared to controls. VEGF levels were determined by electrochemilumiscence Meso Scale Discovery (MULTI-SPOT Assay System) in CSF from age-matched groups of controls with subjective cognitive decline (n = 21), AD without MB (n = 25), AD with MB (n = 25), and VaD (n = 21) patients. The average level of VEGF in the different groups was 2.8 ± 1 pg/ml CSF. Adjusted for age and gender, no significant differences were detected between groups (p | 0.5). However, we detected a significant correlation between the concentration of VEGF in the
Context:Multiple genes and proteins have been shown to be important in treatment and prognosis of breast cancer, including the estrogen and progesterone receptors and Her-2 neu. Vascular endothelial growth factor (VEGF) subtypes have been shown to be associated with lympho-vascular invasion, lymph node metastases and prognosis in multiple types of cancer, including colon cancer, gastric cancer and breast cancer. The goal of this project was to observe and quantify the protein expression of VEGF subtypes in human breast cancer, then correlate this with known clinicopathologic information. Design:Ninety grade three (high grade) invasive ductal carcinomas received over a four year period (1997-2001) were selected from our files. Immunohistochemistry was performed on formalin fixed paraffin embedded tissue of both primary tumor and lymph node metastasis when applicable using VEGF-A, VEGF-C, VEGF-D and VEGF-R (the VEGF receptor) antibodies. The staining was graded from zero (no expression) to 3+ (high
Vascular Endothelial Growth Factor (VEGF), A Major Regulator Of Angiogenesis, Binds To Two Receptor Tyrosine Kinases, KDR/Flk-1 And Flt-1. We Now Describe The Purification And The Expression Cloning From Tumor Cells Of A Third VEGF Receptor, One That
Tumor-induced angiogenesis is of major interest for oncology research. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor characterized so far. VEGF blockade was shown to be sufficient for angiogenesis inhibition and subsequent tumor regression in several preclinical tumor models. Bevacizumab was the first treatment targeting specifically tumor-induced angiogenesis through VEGF blockade to be approved by the Food and Drugs Administration (FDA) for cancer treatment. However, after very promising results in preclinical evaluations, VEGF blockade did not show the expected success in patients. Some tumors became resistant to VEGF blockade. Several factors have been accounted responsible, the over-expression of other angiogenic factors, the noxious influence of VEFG blockade on normal tissues, the selection of hypoxia resistant neoplastic cells, the recruitment of hematopoietic progenitor cells and finally the transient nature of angiogenesis inhibition by VEGF blockade. ...
Vascular endothelial growth factor (VEGF) induces angiogenesis by stimulating endothelial cell proliferation and migration, primarily through the receptor tyrosine kinase VEGF receptor2 (Flk1/KDR). Reactive oxygen species (ROS) derived from NAD(P)H oxidase are critically important in many aspects of vascular cell regulation, and both the small GTPase Rac1 and gp91phox are critical components of the endothelial NAD(P)H oxidase complex. A role of NAD(P)H oxidase in VEGF-induced angiogenesis, however, has not been defined. In the present study, electron spin resonance spectroscopy is utilized to demonstrate that VEGF stimulates O2·− production, which is inhibited by the NAD(P)H oxidase inhibitor, diphenylene iodonium, as well as by overexpression of dominant-negative Rac1 (N17Rac1) and transfection of gp91phox antisense oligonucleotides in human umbilical vein endothelial cells (ECs). Antioxidants, including N-acetylcysteine (NAC), various NAD(P)H oxidase inhibitors, and N17Rac1 significantly ...
In the field of skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio skin substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered skin. Three-dimensional (3D) Gel-C6S-HA scaffolds were prepared by freeze-drying. Vascular endothelial growth factor (VEGF) 165 gene-modified rat HFSCs (rHFSCs) were inoculated into the scaffolds and cultured for 7 days. Two bilateral full-thickness skin defects were created on the back of 18 Sprague-Dawley rats. Rats were randomly divided into four groups: Group A, HFSCs transduced with VEGF165 seeded onto Gel-C6S-HA scaffolds; Group B, HFSCs transduced with empty vector seeded onto Gel-C6S-HA scaffolds; Group C, Gel-C6S-HA scaffold only; Group D, Vaseline gauze dressing. These compositions were implanted onto the defects and harvested at 7, 14 and 21 days. Wound healing was assessed
Purpose: : To evaluate the efficacy and safety of multiple intravitreal injection of recombinant vascular endothelial growth factor receptor-antibody fusion protein (KH902) for diabetic macular edema (DME). Results: : twenty patients (Group A: 10, Group B: 10), including 9 males and 11 females, with mean age of 62.9 years had been enrolled. 16 patients had completed 3 months of core treatment (8 patients in A and B group each). Comparison of improvement in BCVA, CRT and CRV between baseline and after-core treatment showed increment of 7.85 letters in BCVA (58.00+12.39 vs 65.85+10.96), decrease of 94.23mm in CRT (436.46+94.23mm vs 345.29+49.92mm) and decrease of 0.07 mm3 in CRV (0.34+0.08mm3 vs 0.27+0.04mm3). Further analysis indicated that group A gained increment of 5.83 letters in BCVA (57.00+5.06 vs 62.83+6.05), decrease of 95.33mm and 0.08 mm3 in CRT (424.67+106.28mm vs 329.33 +47.81mm) and CRV (0.34+0.08mm3 vs 0.26+0.04mm3), respectively, and that, for B group, increment of 9.57letters in ...
TY - JOUR. T1 - Combination of three angiogenic growth factors has synergistic effects on sprouting of endothelial cell/mesenchymal stem cell-based spheroids in a 3D matrix. AU - Kim, Sook Kyoung. AU - Lee, Jaeyeon. AU - Song, Myeongjin. AU - Kim, Mirim. AU - Hwang, Soon Jung. AU - Jang, Hwanseok. AU - Park, Yongdoo. PY - 2015. Y1 - 2015. N2 - Combinations of angiogenic growth factors have been shown to have synergistic effects on angiogenesis and natural wound healing in various animal models. Each growth factor has unique roles during angiogenesis; vascular endothelial growth factor (VEGF) plays a key role during the initial step of angiogenesis, whereas PDGF functions in the maturation of blood vessels. We used a combination of three angiogenic growth factors to increase angiogenesis in vitro and in vivo. We chose VEGF as a basic factor and added platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) to induce angiogenesis in three in vitro and in vivo models: 3D ...
TY - JOUR. T1 - Capsaicin regulates vascular endothelial cell growth factor expression by modulation of hypoxia inducing factor-1α in human malignant melanoma cells. AU - Patel, Prabhudas S.. AU - Yang, Shillin. AU - Li, Aihua. AU - Varney, Michelle L.. AU - Singh, Rakesh K. PY - 2002/10/9. Y1 - 2002/10/9. N2 - Purpose: Capsaicin (8-Methyl-N-Vanillyl-6-nonenamide), a known natural dietary chemopreventive agent, inhibits malignant melanoma cell proliferation. In the present study, we examined the effect of capsaicin on constitutive and induced vascular endothelial cell growth factor (VEGF) expression in malignant melanoma cells. Results: Capsaicin treatment resulted in enhanced VEGF protein secretion in malignant melanoma cells independent of IL-1β and TNF-α. The observed up-regulation of VEGF production by capsaicin was concentration- and duration-dependent and was inversely associated with inhibition of melanoma cell proliferation. We observed an increase in hypoxia-inducible factor ...
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TY - JOUR. T1 - Fiber type-specific differential expression of angiogenic factors in response to chronic hindlimb ischemia. AU - Cherwek, D. Hunter. AU - Hopkins, M. Benjamin. AU - Thompson, Michael J.. AU - Annex, Brian H.. AU - Taylor, Doris A.. PY - 2000/10/4. Y1 - 2000/10/4. N2 - Alterations in endogenous levels of the angiogenic proteins basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were assessed in rabbit hindlimb muscles subjected to 1, 5, or 21 days of ischemia. In the glycolytic [tibialis anterior (TA)] and the oxidative [soleus (SOL)] muscles from the ischemic and contralateral (control) hindlimb, bFGF and VEGF protein expression was determined by ELISA and immunoblot analysis. Total VEGF protein expression was greater in oxidative than in glycolytic muscles after 5 days of hindlimb ischemia. In SOL muscle, total VEGF detected by ELISA in ischemic limbs was increased to 137, 300, and 220% of control at 1, 5, and 21 days, respectively. However, in ...
To examine whether the lack of sufficient neoangiogenesis in systemic sclerosis (SSc) is caused by a decrease in angiogenic factors and/or an increase in angiostatic factors, the potent proangiogenic molecules vascular endothelial growth factor (VEGF) and basic fibroblast growth factor, and the angiostatic factor endostatin were determined in patients with SSc and in healthy controls. Forty-three patients with established SSc and nine patients with pre-SSc were included in the study. Serum levels of VEGF, basic fibroblast growth factor and endostatin were measured by ELISA. Age-matched and sex-matched healthy volunteers were used as controls. Highly significant differences were found in serum levels of VEGF between SSc patients and healthy controls, whereas no differences could be detected for endostatin and basic fibroblast growth factor. Significantly higher levels of VEGF were detected in patients with Scl-70 autoantibodies and in patients with diffuse SSc. Patients with pre-SSc and short ...
Purpose: Age-related macular degeneration (AMD) is the leading cause of blindness in developed countries. Controversy exists regarding the safety of agents that inhibit vascular endothelial growth factor (VEGF). In this study we performed different assays to compare in vitro cytotoxicity of bevacizumab (B), ranibizumab (R), and aflibercept (A) at clinical doses on retinal pigment epithelia (RPE) cell line (ARPE19).. Methods: Cellular toxicity was assessed through MTT cell viability and BrdU proliferation assays after single or repeated doses of the anti-VEGF drugs. Immunofluorescence (IF) for zonula ocludens (ZO-1) was performed. Intracellular accumulation was determined by IF (antihuman Cy3) one and five days after treatment with B, R and A. IF was also used to determine the activity of caspase-3 after single or repeated doses of B, R and A. The effect of anti-VEGF agents on cell phagocytosis of fluorescent latex beads was also assessed. Finally, RPE permeability 24, 48 and 72 hours after ...
Vascular endothelial growth factor A (VEGF-A) is a protein that in humans is encoded by the VEGFA gene. This gene is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGF-A shows prominent activity with vascular endothelial cells, primarily through its interactions with the VEGFR1 and -R2 receptors found in prominently on the endothelial cell membrane. Although, it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, ...
The well-established role of vascular endothelial growth factor (VEGF) in carcinogenesis and tumor angiogenesis has led to the development of agents that target this pathway. Anti-VEGF agents the VEGF monoclonal antibody bevacizumab, and the small molecule VEGF receptor tyrosine kinase inhibitors. Angiogenic factors play a key role in the maintenance of lung integrity and normal endothelial function. Endothelial dysfunction has been implicated in hypertension, proteinuria and retinopathy. One of the major issues of anti-VEGF agents is its long-term toxicity especially taking into account the lack of adequate knowledge in this area and the possibility of prolonged periods of therapy in non-progressing patients. Hypertension and proteinuria are commonly seen in patients treated with anti-VEGF agents. In addition, we have also observed in a relatively high frequency of pulmonary air-filled lesions in patients with malignancy in the lung treated with an anti-VEGF agent. Objectives of this ...
Neovascularization is essential for tumour growth and is mediated by physiological substances produced by tumours. Vascular endothelial growth factor (VEGF) is one such potent angiogenic factor. Human gliomas, the most important class of intrinsic brain tumours, express VEGF both in vivo and in vitro. Factors involved in the control of VEGF production by glioma cells are not well known. In this study, we investigated the role of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and platelet derived growth factors (PDGF) on VEGF production by four different glioma cell lines in vitro. With the exception of PDGF A/A and B/B in one cell line, all growth factors differentially stimulated VEGF production in all cell lines investigated. These data suggest that VEGF production in human glioma may be regulated by other growth factors which are also known to be expressed in such tumours ...